Environmental Research最新文献

{"title":"Decadal variability and anthropogenic influence on trace element dynamics in the Bay of Bengal: Evidence from high-resolution coral records","authors":"Md Habibur Rahman ,&nbsp;Tianran Chen ,&nbsp;Syeda Maksuda Yeasmin ,&nbsp;Yuyang Lin ,&nbsp;Jianxin Zhao ,&nbsp;Fabrice Papa ,&nbsp;Md Hafijur Rahaman Khan","doi":"10.1016/j.envres.2025.121828","DOIUrl":"10.1016/j.envres.2025.121828","url":null,"abstract":"<div><div>The Bay of Bengal (BoB) exhibits dynamic trace element (TE) chemistry shaped by a complex interplay of natural processes and anthropogenic influences. Despite its significance, the temporal variability of TEs in this region is poorly understood. This study investigates decadal (2011–2022) TE dynamics by analyzing trace element-to-calcium ratios (TEs/Ca: B/Ca, Mn/Ca, Fe/Ca, Ba/Ca, P/Ca, As/Ca, Zn/Ca, Cd/Ca, and Sn/Ca) and strontium-to-calcium (Sr/Ca) ratios in modern <em>Porites</em> corals, supplemented by data from a sub-fossil coral as a benchmark. The results reveal distinct seasonal cycles in B/Ca, Sr/Ca, and Ba/Ca, with B/Ca and Sr/Ca being predominantly controlled by sea surface temperature (SST), while Ba/Ca variability is influenced by terrestrial inputs associated with river discharge. B/Ca has been validated as a reliable SST proxy for riverine runoff-influenced BoB. Elevated Fe/Ca, Mn/Ca, and As/Ca ratios are associated with anthropogenic activities, such as the construction of significant infrastructure projects (e.g., Padma Multipurpose Bridge, Payra Sea Port, and Matarbari Deep Sea Port) and the establishment of the Rohingya refugee camp. Additionally, increases in P/Ca, Zn/Ca, and Cd/Ca reflect intensified fertilizer use, whereas rising Sn/Ca levels suggest inputs from antifouling agents, pesticides, and tourism. Principal component analysis combined with multiple linear regression (PCA-MLR) indicates that anthropogenic activities account for approximately 70 % of the TE variability. These findings underscore the significant role of human activities in altering the TE dynamics in the BoB, providing valuable insights for coastal management and pollution mitigation strategies in similarly impacted regions worldwide.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"279 ","pages":"Article 121828"},"PeriodicalIF":7.7,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex-specific associations between per- and polyfluoroalkyl substance exposure and epigenetic age: Findings from the National Health and Nutrition Examination survey 1999–2000","authors":"Dennis Khodasevich ,&nbsp;Anne K. Bozack ,&nbsp;Belinda L. Needham ,&nbsp;David H. Rehkopf ,&nbsp;Andres Cardenas","doi":"10.1016/j.envres.2025.121827","DOIUrl":"10.1016/j.envres.2025.121827","url":null,"abstract":"<div><div>Per-and polyfluoroalkyl substances (PFAS) are a pervasive family of synthetic compounds with a wide range of reported health effects. Epigenetic clocks, DNA methylation-based predictors of chronological and biological age, are promising biomarkers for characterizing biological aging in humans. The potential impact of PFAS exposure on epigenetic aging in the general US population remains unclear. In the 1999–2000 National Health and Nutrition Examination Survey (NHANES) cycle (N = 262), eleven PFASs were measured in serum and DNA methylation was measured in blood with the EPICv1 array. Seven epigenetic clocks and their respective epigenetic age acceleration (EAA) measures were calculated. Survey-design weighted generalized linear regression models were used to test adjusted associations between individual log₂-transformed PFAS concentrations and EAA stratified by sex. Among male participants, doubling of PFNA concentrations was associated with greater EAA across several clocks including the Horvath clock (beta = 1.48, 95 % CI: 0.35, 2.61), Skin&amp;Blood clock (beta = 1.27, 95 % CI: 0.21, 2.32), and PhenoAge (beta = 1.43, 95 % CI: 0.41, 2.44), and doubling of PFOS exposure was associated with greater Skin&amp;Blood EAA (beta = 1.14, 95 % CI: 0.04, 2.24). When considering cell-adjusted EAA measures, each of these associations among male participants remained significant, and PFOSA was associated with decreased PhenoAge EAA (beta = −0.84, 95 % CI: −1.49, −0.18) and GrimAge2 EAA (beta = −0.81, 95 % CI: −1.51, −0.11) among female participants. In summary, we found evidence of sex-specific associations between PFAS exposure and epigenetic aging in a sample of older adults representative of the general US population.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"279 ","pages":"Article 121827"},"PeriodicalIF":7.7,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of a real-time reverse transcription polymerase chain reaction for rapid detection of Escherichia coli in drinking water: an EU representative study","authors":"Livia Gómez ,&nbsp;João Brandão ,&nbsp;Anna Navarro ,&nbsp;Antonino Martines ,&nbsp;Isabella Sanseverino ,&nbsp;Elena Porcel-Rodríguez ,&nbsp;Dimitar Marinov ,&nbsp;Oksana Ableitner ,&nbsp;Franz Allerberger ,&nbsp;Adrie Atsma ,&nbsp;Rossella Briancesco ,&nbsp;Arijana Cenov ,&nbsp;Marianna Cíchová ,&nbsp;Anna Maria Coccia ,&nbsp;Simonetta Della Libera ,&nbsp;Katrien De Maeyer ,&nbsp;Hendrik Jan de Vries ,&nbsp;Goffe Elsinga ,&nbsp;Leo Heijnen ,&nbsp;Anna-Maria Hokajärvi ,&nbsp;Teresa Lettieri","doi":"10.1016/j.envres.2025.121786","DOIUrl":"10.1016/j.envres.2025.121786","url":null,"abstract":"<div><div>The microbiological quality of water intended for human consumption in the EU is regulated by the recast Drinking Water Directive 2020/2184 (DWD), which sets strict parametric values for intestinal enterococci and <em>Escherichia coli</em> (<em>E. coli</em>), allowing no more than zero colony-forming units per 100 mL. Detection and enumeration of <em>E. coli</em> typically rely on culture-based reference methods or the most probable number approach, which require 1–2 days to produce results—potentially delaying timely action during contamination events. In contrast, molecular techniques can deliver results within hours. The DWD permits the use of alternative methods if they are as reliable as the reference method and developing and validating such methods requires multiple laboratory trials in line with ISO standard 16140-2. Following this, we conducted a representative EU study to validate a molecular method based on real-time reverse transcription polymerase chain reaction for rapid <em>E. coli</em> detection in drinking water. In a concerted action, the first of its kind for drinking water, nineteen laboratories across ten Member States participated. To ensure consistency, drinking water was artificially contaminated with <em>E. coli</em>. The alternative method showed slightly lower sensitivity than the reference method (91.1 % vs. 97.2 %) but delivered much faster results, making it a valuable screening tool. It can support rapid decision-making during contamination events, reducing the risk of waterborne outbreaks and protecting public health. For reliable routine performance, appropriate training in this alternative method is strongly recommended.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"279 ","pages":"Article 121786"},"PeriodicalIF":7.7,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143936380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thermophilic degradation of sulfamethazine by Geobacillus sp. S-07: pathway and mechanism","authors":"Ting Pan ,&nbsp;Yan-Yan Zhou ,&nbsp;Xu Huang ,&nbsp;Jian-Xin Xu ,&nbsp;Xiao-Yu Guo ,&nbsp;Jian-Qiang Su ,&nbsp;Hu Li ,&nbsp;Xiao-Ru Yang","doi":"10.1016/j.envres.2025.121823","DOIUrl":"10.1016/j.envres.2025.121823","url":null,"abstract":"<div><div>Biodegradation is crucial for the removal and remediation of sulfonamide antibiotic (SA) contamination. Comprehensively understanding the thermophilic degradation mechanism is essential for the application of SA-biodegrading isolates in engineered systems, such as composting. In this study, we explored the thermophilic biodegradation mechanism of <em>Geobacillus</em> sp. S-07 on sulfamethazine (SMZ). Targeted metabolite analysis unveiled that strain S-07 effectively detoxifies SMZ by modifying the amino moiety and disassembling the sulfonamide bridge moiety. By integrating genomic and proteomic analysis, enzymes potentially involved in the SMZ biotransformation were further proposed, including an adenine deaminase, a dimethylsulfone monooxygenase, and a putative heme-containing peroxidase. Genomic analysis indicated that S-07 carries five antibiotic resistance genes, presenting a low mobility in horizontal transfer, implying its low resistance pollution risk in bioremediation application. This study offers novel insights into the thermophilic SA biodegradation mechanism, and provides biological resources for the development of thermophilic bioremediation technologies aimed at enhanced SA removal.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"279 ","pages":"Article 121823"},"PeriodicalIF":7.7,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early life phthalate exposure impacts gray matter and white matter volume in infants and young children.","authors":"Emily J Werder, Kun Lu, Chih-Wei Liu, Jake E Thistle, Julia E Rager, Gang Li, Zhengwang Wu, Tengfei Li, Li Wang, Dale P Sandler, John H Gilmore, Joseph Piven, Hongtu Zhu, Weili Lin, Stephanie M Engel","doi":"10.1016/j.envres.2025.121826","DOIUrl":"https://doi.org/10.1016/j.envres.2025.121826","url":null,"abstract":"<p><strong>Objective: </strong>Prenatal phthalate exposure is associated with adverse neurodevelopmental outcomes, yet data on impacts of early life exposure remains limited. We investigated phthalate and replacement plasticizer exposures from 2 weeks to 7 years of age in relation to brain anatomical attributes, using serial structural magnetic resonance imaging (sMRI).</p><p><strong>Material and methods: </strong>Children were enrolled after birth into the UNC Baby Connectome Project, a longitudinal neuroimaging study (North Carolina, USA; 2017-2020). Urine samples (n=406) were collected at each visit and analyzed for 17 phthalate and replacement plasticizer metabolites. Among 157 children contributing 369 sMRIs, we calculated metabolite-specific average exposures across each individual's urine samples and used linear mixed models to estimate longitudinal associations of log transformed, specific gravity-adjusted average metabolite concentrations with gray and white matter volume, and cortical volume, thickness, and surface area. We examined sex-specific differences in these associations.</p><p><strong>Results: </strong>Higher average metabolite concentration was associated with lower gray matter volume (MCPP: (-1.73 cm³, 95% CI: -3.36, -0.10) and higher white matter volume (∑DEHP: 2.28 cm³, 95% CI: 0.08, 4.48). Among boys (n=72, 140 sMRIs), MEP (-2.97 cm³, 95% CI: -5.85, -0.09) and MiBP (-2.40 cm³, 95% CI: -4.64, -0.15) were also associated with lower gray matter volume. Among females (n=85, 229 MRIs), higher ∑DINCH exposure was associated with higher white matter volume (2.27 cm³, 95% CI: 0.29, 4.25). We observed significant sex interactions for ∑DEHP with gray matter (p-interaction=0.03) and ∑DINCH with white matter volume (p-interaction=0.001).</p><p><strong>Conclusion: </strong>Early life phthalate/plasticizer exposure may differentially impact various brain region volumes in early childhood, with potential downstream consequences on functional development.</p>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":" ","pages":"121826"},"PeriodicalIF":7.7,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Per- and polyfluoroalkyl substances (PFAS) and microRNA: An epigenome-wide association study in firefighters","authors":"Melissa A. Furlong ,&nbsp;Tuo Liu ,&nbsp;Alesia Jung ,&nbsp;Shawn Beitel ,&nbsp;Jeff Hughes ,&nbsp;Randy Krause ,&nbsp;Judith M. Graber ,&nbsp;Miriam M. Calkins ,&nbsp;Antonia M. Calafat ,&nbsp;Julianne C. Botelho ,&nbsp;Matthew Huentelman ,&nbsp;John Gulotta ,&nbsp;Jaclyn M. Goodrich ,&nbsp;Jefferey L. Burgess","doi":"10.1016/j.envres.2025.121766","DOIUrl":"10.1016/j.envres.2025.121766","url":null,"abstract":"<div><div>The occupation of firefighting is classified as a Group 1 carcinogen. Increased cancer risk among firefighters may be partly attributable to increased occupational exposure to a range of chemicals, including per- and polyfluoroalkyl substances (PFAS). Some PFAS are known and suspect human carcinogens. Investigating epigenetic response to these PFAS exposures in firefighters may help to identify biological pathways of specific cancers, and previously unidentified health outcomes that are associated with PFAS. We therefore investigated the associations of serum PFAS concentrations with miRNA expression in firefighters. Serum samples collected from 303 firefighters from 6 sites across the USA were analyzed for 9 PFAS along with miRNA expression. Covariate-adjusted linear regression was used to estimate associations between log PFAS and miRNA expression, with false discovery rate (FDR) set to 0.05 for significance, and an exploratory cutoff of FDR q &lt; 0.20. Gene set enrichment analysis (GSEA) was performed using miRTarBase's miRWalk pathways. Age, race-ethnicity, BMI, fire department, and sex were controlled for in all models. At FDR&lt;0.05, the linear isomer of perfluorooctane sulfonic <em>acid (PFOS)</em> was inversely associated with miR-128-1-5p expression (Beta = −0.146, 95 % CI -0.216, −0.076). At a relaxed FDR of 0.20, we observed inverse associations for the sum of branched isomers of PFOS (Sm-PFOS) with 5 miRNAs (let-7d-5p, let-7a-5p, miR-423-5p, let-7b-5p, miR-629-5p). Several pathways were enriched for multiple PFAS, including those correlated with certain cancers, blood diseases, thyroid disorders, autoimmune disorders, and neurological outcomes. Some PFAS in firefighters were found to be associated with alteration of miRNA consistent with increased risk for a range of chronic diseases.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"279 ","pages":"Article 121766"},"PeriodicalIF":7.7,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of inhomogeneous bismuth-based heterojunctions with efficient interfacial charge transport: Bridging of BiOX","authors":"Yuanting Wu ,&nbsp;Lihui Guo ,&nbsp;Xuhua Liu ,&nbsp;Xiaoying Wang ,&nbsp;Jingyue Hu ,&nbsp;Yunlong Xue ,&nbsp;Ou Hai ,&nbsp;Hulin Liu ,&nbsp;Xinmeng Zhang ,&nbsp;Weizhi Tian ,&nbsp;Yongqiang Feng","doi":"10.1016/j.envres.2025.121821","DOIUrl":"10.1016/j.envres.2025.121821","url":null,"abstract":"<div><div>The inefficiency of photogenerated charge transfer between heterojunction interfaces imposes significant limitations on the activity of photocatalytic materials. In this paper, Bi₂WO₆-Bi₂O₂SiO₃-Bi₁₂SiO₂₀-BiOXY (X = Cl, Br, Y=I) (BSBI and BSCI) heterojunctions were successfully constructed by utilizing the structural similarity of Bi-based materials. The effects of different halide species, concentrations and volume ratios on their photocatalytic performance were investigated. The results showed that the optimized BSBI heterojunction obtained by multilayer substitution exhibited excellent photocatalytic activity, which achieved up to 99.3 % degradation of rhodamine B (Rh B, 10 mg/L) under 60 min of simulated sunlight, 85.4 % degradation of norfloxacin (NFX, 10 mg/L) within 120 min and 85.3 % degradation of tetracycline hydrochloride (TCH, 10 mg/L) within 120 min. The enhanced photocatalytic performance was attributed to the ion-exchange-induced multilayer replacement. The generated BiOX effectively solved the problem of insufficient charge transfer driving force caused by the minor potential difference and the large interfacial resistance at the heterogeneous interfaces of Bi₂O₂SiO₃-Bi₁₂SiO₂₀ and Bi₂WO₆ through the “bridging” effect, which significantly improved the carrier transport efficiency between the heterojunctions. This work provides a valuable methodology and basis for constructing bismuth-based multi-heterojunction interfaces with efficient charge transfer.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"279 ","pages":"Article 121821"},"PeriodicalIF":7.7,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Total nitrogen levels as a key constraint on soil organic carbon stocks across Australian agricultural soils.","authors":"Huirong Jing, Alexis Pang, Senani Karunaratne, Baobao Pan, Xia Liang, Dorin Gupta, Deli Chen","doi":"10.1016/j.envres.2025.121825","DOIUrl":"https://doi.org/10.1016/j.envres.2025.121825","url":null,"abstract":"<p><p>Understanding how pedoclimatic drivers regulate soil organic carbon (SOC) stock is crucial for gaining insights into terrestrial carbon-climate feedback and thus adaptation to climate change. However, current data-driven SOC predictive models often neglect to incorporate total nitrogen (TN) data, thereby constraining our understanding of carbon-nitrogen interactions and their influence on SOC storage mechanisms across large scales. Utilizing an interpretable machine learning technique, we investigate how key drivers (TN, climate, elevation, land use, pH, SiO₂) affect SOC stocks at different soil depths across Australian major agricultural production regions. Incorporating TN into data-based SOC predictive models enhanced the explained variation by approximately 11%. TN was identified as the predominant factor influencing SOC stocks, accounting for over 47% of observed variability across all depths and outweighing climate effects in subsurface soils. Furthermore, we identified depth-specific thresholds of TN levels that constrain SOC accumulation: 1.45 mg/g soil for 0∼10 cm, 0.80 mg/g soil for 10∼20 cm and 0.63 mg/g soil for 20∼30 cm. Projections of SOC stocks under different scenarios suggest that achieving these TN thresholds can promote SOC accumulation and help offset SOC losses associated with a 1°C increase in mean annual temperature. This study underscores TN levels as a key constraint on SOC stocks across Australian agricultural soils, and thus should be explicitly considered when predicting large-sale SOC dynamics and formulating soil carbon sequestration strategies.</p>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":" ","pages":"121825"},"PeriodicalIF":7.7,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complex resistivity dispersion for monitoring soil contaminated by selected organic pollutants","authors":"Diquan Li ,&nbsp;Weitian Wu ,&nbsp;Hanyu Zhu ,&nbsp;Weiliang Guo ,&nbsp;Yecheng Liu ,&nbsp;Ning Li","doi":"10.1016/j.envres.2025.121756","DOIUrl":"10.1016/j.envres.2025.121756","url":null,"abstract":"<div><div>With the rapid development of the global petrochemical industry and the widespread use of organic solvents, the problem of non-aqueous phase pollution of soil is becoming increasingly serious. Due to the significant differences in complex resistivity among various pollutants, studying the complex resistivity dispersion characteristics of different pollution types and degrees can help evaluate contaminated sites and is highly significant for pollution control. In this study, soil samples with different concentrations of trichloroethylene, xylene and gasoline were prepared, the complex resistivity dispersion curves of each group were measured, the electrical parameters were obtained by inversion. The effectiveness of the indoor experiment was verified by the field pollution delineation experiment. The results reveal the strong correlation between the degree of soil organic matter pollution, the type of pollution and the complex resistivity dispersion curve, and clarify the internal relationship between the type of pollution, the degree of pollution and the electrical parameters. Among them, the real part resistivity can effectively reflect the resistivity difference of contaminated soil, and the imaginary part resistivity can distinguish the characteristics of pollution degree and pore water composition. Based on theoretical analysis, sample determination and field test, this paper proves the effectiveness of using complex resistivity dispersion characteristics as an evaluation method for non-aqueous soil pollution, and provides a basis for complex resistivity method in the field of organic pollution monitoring and evaluation.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"279 ","pages":"Article 121756"},"PeriodicalIF":7.7,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143941551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and metabolic analysis of domesticated anaerobic bacteria for effective thiamethoxam bioremediation: Enzymatic, genetic, and pathway insights","authors":"Jun Tu ,&nbsp;Qihang Huang ,&nbsp;Qiuru Chen ,&nbsp;Yuhan Zhou ,&nbsp;Xiaohui Yi ,&nbsp;Yiquan Qiu ,&nbsp;Xinzhi Wang ,&nbsp;Xiaotong Bai ,&nbsp;Chao Zhang ,&nbsp;Mingzhi Huang","doi":"10.1016/j.envres.2025.121813","DOIUrl":"10.1016/j.envres.2025.121813","url":null,"abstract":"<div><div>The extensive use of neonicotinoid insecticides (NEOs), particularly thiamethoxam (THM), poses significant risks to both the ecological environment and human health, emerging as a critical environmental issue that requires immediate attention. In this study, anaerobic bacteria were successfully domesticated under THM stress. The results showed that anaerobic bacteria could effectively degrade THM within 7 days, with the degradation efficiency reaching 20.0–32.3 % under different conditions. Bacterial community analysis identified <em>Escherichia-Shigella</em>, <em>Acinetobacter</em>, <em>Delftia</em>, <em>Enterobacter</em>, and <em>Pseudomonas</em> as the dominant genera at the genus level. During the THM degradation process, a decline in bacterial urease activity was observed, accompanied by a rise in catalase (CAT) activity, while β-galactosidase activity remained stable. Functional gene analysis of the macro-transcriptome revealed significant upregulation of genes related to the citrate cycle (TCA), oxidative phosphorylation, protein translocation, nitrogen metabolism, and the bacterial secretion system. Density functional theory (DFT) calculations and LC-MS/MS analyses identified three potential degradation pathways for THM, with nine characterized intermediates. In conclusion, the degradation performance and mechanism of THM by anaerobic bacteria were investigated for the first time. This study provides a theoretical basis for the biological treatment and ecological remediation of neonicotinoid pesticide wastewater.</div></div>","PeriodicalId":312,"journal":{"name":"Environmental Research","volume":"279 ","pages":"Article 121813"},"PeriodicalIF":7.7,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}