Environmental Pollution最新文献

{"title":"N-acyl-homoserine-lactones as a critical factor for biofilm formation during the initial adhesion stage in drinking water distribution systems","authors":"Ruisi Chen, Ruotong Xu, Jiaxin Huang, Xiuneng Zhu, Yulin Tang, Yongji Zhang","doi":"10.1016/j.envpol.2024.125489","DOIUrl":"https://doi.org/10.1016/j.envpol.2024.125489","url":null,"abstract":"The N-acyl-homoserine-lactone (AHLs)-mediated quorum sensing (QS) system is crucial for the coordination of microbial behaviors within communities. However, the levels of AHLs in biofilms in drinking water distribution systems (DWDSs) and their impact on biofilm formation remain poorly understood. Herein, we simulated DWDSs via biofilm reactors to explore the presence and influence of AHLs during the initial stages of biofilm formation on pipe walls. Glass, polypropylene random copolymer (PP-R) and stainless steel (SS) were used as the coupon materials and the three parallel experimental groups were set up and named accordingly. The glass material is considered to form biofilms only minimally and is therefore used as a negative control. By day 30, the concentration of AHLs in biofilm phase in both PP-R group and SS group reached 1200-1800 ng/L. The predominant AHLs were C6-HSL, C8-HSL, and C10-HSL, with a significant positive correlation between AHLs and biofilm biomass. Metagenomic analysis revealed that microbes exhibiting significant differences among the three groups all demonstrated notable correlations with AHLs. Subsequent analysis of QS genes revealed that the genes associated with AHLs biosynthesis and QS receptors were more abundant in the PP-R and SS groups with biofilm formation. Additionally, we analyzed the abundance of genes related to cell motility, transmembrane transport, tricarboxylic acid cycle, and genetic information synthesis. The co-occurrence network indicates that these processes exhibit a strong correlation with QS genes. This study demonstrates the pivotal role of AHLs in microbial communication during the initial stages of biofilm formation in DWDSs and indicates that the regulatory pathways and mechanisms of AHLs may vary under different environmental conditions.","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"147 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142788584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential Impact of Wildfire and Rice Straw Ash on the Skin Microbiota of Rana dybowskii Adults and Tadpoles","authors":"Xin-zhou Long, Ming-da Xu, Wen-jing Dong, Xiu-mei Yang, Li-yong Cui, Qing Tong","doi":"10.1016/j.envpol.2024.125470","DOIUrl":"https://doi.org/10.1016/j.envpol.2024.125470","url":null,"abstract":"Global warming has intensified severe weather conditions and increased the frequency of wildfires, posing significant threats to ecosystems. Moreover, rice straw ash, a byproduct of human agricultural activities, represents an environmental stressor that can further impact these vulnerable ecosystems. These changes particularly impact sensitive species and microorganisms, yet limited research has explored the effects of wildfire ash and agricultural byproducts, such as rice straw ash, on amphibians. This study aims to investigate the effects of both wildfire and rice straw ashes on the skin microbiota of <em>Rana dybowskii</em> tadpoles and adult frogs, using ash aqueous extracts (AEAs). While alpha diversity showed significant variation among tadpoles, it remained stable in adult frogs. Beta diversity analyses revealed distinct microbiota compositions, especially between control and wildfire ash-treated tadpoles. Linear discriminant analysis (LDA) Effect Size (LEfSe) analysis indicated that different ash treatments led to the enrichment of specific microbiota, reflecting the complex effects of environmental changes on amphibian skin microbiota. Specific bacterial enrichments were associated with each treatment group, and phenotypic analysis highlighted bacterial traits, including Aerobic, Anaerobic, Potentially_Pathogenic, and Stress_Tolerant, providing insights into ecological adaptations. Therefore, contrasts wildfire and rice straw ash treatments distinctly influence amphibian skin microbiota and associated bacterial traits. Our findings emphasize the impact of agricultural and wildfire ash on amphibian skin microbiota, offering key ecological insights into the challenges posed by global environmental changes.","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"1 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142788581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ten years of monitoring pharmaceuticals and pesticides in the aquatic environment nearby a solid-waste treatment plant: historical data, trends and risk assessment","authors":"Claudia Simarro-Gimeno, Elena Pitarch, Fernando Albarrán, Andreu Rico, Félix Hernández","doi":"10.1016/j.envpol.2024.125496","DOIUrl":"https://doi.org/10.1016/j.envpol.2024.125496","url":null,"abstract":"The emission of contaminants of emerging concern (CECs) from wastewater treatment plants has been extensively studied; however, less attention has been paid to municipal solid waste treatment plants (MSWTPs), which can also be a potential source for CECs into surface water (SW) and groundwater (GW) ecosystems. In this work, the environmental impact of a MSWTP located in the province of Castelló, Spain, was studied along a period of ten years (from 2012 to 2022). A total of 173 water samples (including SW and GW) collected from the surrounding of this plant were monitored for 93 compounds (pharmaceuticals and pesticides) by using liquid chromatography coupled to tandem mass spectrometry with triple quadrupole. This study reveals the presence of several pharmaceuticals (e.g. primidone, gabapentin, azithromycin, clarithromycin, tramadol), particularly in GW samples collected near areas related to composting and storage of biostabilized material. The presence of antibiotic residues in GW raises concerns about the potential development of antimicrobial resistance. In addition, agricultural activities in the study area emerge as potential contributor to GW pollution by pesticides, as the MSWTP is located in an important agricultural area where citrus is the predominant crop. Some compounds that are currently prohibited for agricultural use (e.g. atrazine, simazine, chlorpyrifos) were also found, which highlights the importance of continuing their monitoring to assess their long-term environmental impacts. Several pesticide and pharmaceutical compounds exceeded the threshold values established by the EU groundwater directive. Therefore, a hazard assessment for GW ecosystems and for humans drinking contaminated GW resources was conducted. Our data indicated that some organophosphate insecticides (i.e., chlorpyrifos, carbofuran, pyridaphention) may pose high risks for groundwater crustaceans, while the risks for the human population were considered to be very low.","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"17 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142788582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidating the hydrochemistry and REE evolution of surface water and groundwater affected by acid mine drainage","authors":"Xiaoxin Shi, Yanyan Gao, Hui Qian, Jie Chen, Weiqing Li, Siqi Li, Yixin Liu","doi":"10.1016/j.envpol.2024.125495","DOIUrl":"https://doi.org/10.1016/j.envpol.2024.125495","url":null,"abstract":"The impact of pyrite mining on water quality is a global concern. This study investigates the impact of acid mine drainage (AMD) from an abandoned pyrite mine in the Qinling Mountains on surface and groundwater hydrochemistry and rare earth elements (REEs) evolution. A total of 54 water samples were collected in 2021, of which the Muzi River downstream of the mining area was repeated three times in three sampling periods. Hydrogeochemical methods and stable isotope techniques were used to analyze the impacts of AMD. Results showed that tailing water in comparison to groundwater and surface waters exhibits low pH with high concentrations of SO₄^2-, potentially toxic elements (PTEs), and REEs, and is characterized by normalized middle REE (MREE) enrichment. Groundwater is less influenced by AMD and shows HCO₃-Ca and HCO₃-Ca·Na types. AMD contaminates surface water to different degrees. Surface water received SO₄^2- input from AMD, exhibited SO₄-Ca, SO₄·HCO₃-Ca, and HCO₃·SO₄-Ca types within the mining area, and evolved from HCO₃·SO₄-Ca to HCO₃-Ca downstream as AMD influence diminishes. High concentrations of PTEs and REEs are presented in AMD and seepage near the slag heap, and decreased rapidly along the flow path, while SO₄^2- migrated over longer distances. The water in the study area primarily originates from atmospheric precipitation, with close relation among surface water, groundwater, and tailing water. Water-rock interactions and pyrite oxidation governed the hydrochemical composition, with sulfide oxidation facilitated the carbonatite-water reaction, which alleviated sulfide oxidation-induced acidification. The concentrations of PTEs are regulated by adsorption and precipitation, carbonate buffering, and dilution along the flow path. REEs are mainly controlled by pH, inorganic complexation, and secondary mineral adsorption. As the pH changes from acidic to neutral or weakly alkaline, REEs shift from sulfate-complex dominated to carbonate-complex dominated. These insights contribute to a better understanding of AMD impacts on surface and groundwater, providing a basis for the rational management of AMD.","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"60 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142788583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effects of carbon and heat under disturbance of human activities: Evidence from a resource-based city of China","authors":"Yaping ZHANG, Jianjun ZHANG, Wei CHEN, Sen LIANG, Kexin YI, Shidong LIU","doi":"10.1016/j.envpol.2024.125424","DOIUrl":"https://doi.org/10.1016/j.envpol.2024.125424","url":null,"abstract":"For resource-based cities, the rapid development of industrialization and urbanization has led to significant carbon emissions (CEs), accelerated the rise of urban land surface temperatures (LSTs) and hindered sustainable urban development. This study constructed a model to measure the carbon-heat relationship to clarify the complex relationship between LSTs and CEs in resource-based cities. The results show that:1) High-temperature areas are primarily concentrated around the urban center and large industrial zones, with average LSTs reaching a peak of 35.7°C in 2015, indicating severe temperature polarization; 2) CEs exhibited an overall upward trend with a diffusion effect, particularly pronounced in the urban center and industrial zones. Areas with extremely significant, strong significant, and generally significant growth in CEs accounted for 4.64%, 3.81%, and 81.35%, respectively, showing a concentrated increase in the urban center; 3) A positive correlation between CEs and LSTs of the city was identified, and the distribution of urban heat island and the high value area of CEs are concentrated and similar; 4) The synergistic effects between LSTs and CEs varied between urban center, suburban and peripheral areas, due to human activities. Areas with a high positive correlation between CEs and LSTs are concentrated in urban centers and peripheral areas, while for urban suburbs, the correlation is weak or even absent. To mitigate the negative effects of carbon-heat accumulation, urban centers should avoid high population concentrations, and the carbon sink potential of green spaces near industrial zones and peripheral areas should be fully utilized. These insights provide actionable strategies for sustainability of resource-based cities, particularly in the governance of urban thermal environments and the mitigation of CEs.","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"37 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142788755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-scale influences on Escherichia coli concentrations in shellfish: from catchment to estuary","authors":"Shelagh K. Malham, Helen Taft, Kata Farkas, Cai J.T. Ladd, Mathew Seymour, Peter E. Robins, Davey L. Jones, James E. McDonald, Lewis Le Vay, Laurence Jones","doi":"10.1016/j.envpol.2024.125476","DOIUrl":"https://doi.org/10.1016/j.envpol.2024.125476","url":null,"abstract":"Sustainability of bivalve shellfish farming relies on clean coastal waters, however, high levels of faecal indicator organisms (FIOs, e.g. <em>Escherichia coli</em>) in shellfish results in temporary closure of shellfish harvesting beds to protect human health, but with economic consequences for the shellfish industry. Active Management Systems which can predict FIO contamination may help reduce shellfishery closures. This study evaluated predictors of <em>E. coli</em> concentrations in two shellfish species, the blue mussel (<em>Mytilus edulis</em>) and the Pacific oyster (<em>Crassostrea gigas</em>), at different spatial and temporal scales, within 12 estuaries in England and Wales. We aimed to: (i) identify consistent catchment-scale or within-estuary predictors of elevated <em>E. coli</em> levels in shellfish, (ii) evaluate whether high river flows associated with rainfall events were a significant predictor of shellfish <em>E. coli</em> concentrations, and the time lag between these events and <em>E. coli</em> accumulation, and (iii) whether operation of Combined Sewer Overflows (CSO) is associated with higher <em>E. coli</em> concentrations in shellfish. A cross-catchment analysis gave a good predictive model for contamination management (R² = 0.514), with positive relationships between <em>E. coli</em> concentrations and river flow (p=0.001), turbidity (p=0.002) and nitrate (p=0.042). No effect was observed for catchment area, the number of point source discharges, or agricultural land use type. 64% of all shellfish beds showed a significant relationship between <em>E. coli</em> and river flow, with typical lag-times of 1-3 days. Detailed analysis of the Conwy estuary indicated that <em>E. coli</em> counts were consistently higher when the CSO had been active the previous week. In conclusion, we demonstrate that real-time river flow and water quality data may be used to predict potential risk of <em>E. coli</em> contamination in shellfish at the catchment level, however, further refinement (coupling to fine-scale hydrodynamic models) is needed to make accurate predictions for individual shellfish beds within estuaries.","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"10 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142788580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"p38 MAPK determines the sensitivity of the aquatic keystone species Moina macrocopa to toxic Microcystis: Insights into potential biomarker applications","authors":"Kai Lyu, Yuchen Fan, Xinyu Zhou, Yuzhou Hou, Zhou Yang","doi":"10.1016/j.envpol.2024.125458","DOIUrl":"https://doi.org/10.1016/j.envpol.2024.125458","url":null,"abstract":"Harmful cyanobacterial blooms, particularly those caused by <em>Microcystis</em> species, pose significant ecological threats to freshwater environments by negatively impacting zooplankton populations, essential components of aquatic food webs. Understanding the molecular mechanisms underlying zooplankton responses to these toxic blooms is crucial for assessing and mitigating these impacts. The mitogen-activated protein kinase (MAPK) pathway, known for its critical role in stress response signaling, offers a promising area of study to elucidate these mechanisms. However, the specific involvement of MAPK in zooplankton responses to cyanobacterial stress remains unclear. In this study, we identify and characterize the p38 MAPK gene (MmMAPK) from the zooplankton <em>Moina macrocopa</em>. The gene contains conserved structural elements typical of MAPKs, including a Thr-Gly-Tyr (TGY) motif and a substrate-binding site, Ala-Thr-Arg-Trp (ATRW), indicating its potential functional relevance in stress signaling pathways. Expression analysis reveals a significant upregulation of MmMAPK in <em>M. macrocopa</em> upon exposure to toxic <em>Microcystis</em>, suggesting its role in mediating the organism's stress response. Furthermore, RNA interference (RNAi) experiments demonstrate that knockdown of MmMAPK results in reduced survival and decreased body size, particularly under cyanobacterial stress, underscoring its importance in maintaining stress sensitivity. These findings provide new insights into the molecular mechanisms by which <em>M. macrocopa</em> responds to harmful algal blooms and highlight the potential of MmMAPK as a biomarker for ecological risk assessment and management of cyanobacterial pollution in freshwater ecosystems.","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"13 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"2,4-bisphenol S triggers physiological changes, oxidative stress and lipidome alterations in Gram-positive Enterococcus faecalis at environmental concentrations","authors":"Pengyu Chen, Baihui An, Yuxi Hu, Yuqiang Tao","doi":"10.1016/j.envpol.2024.125475","DOIUrl":"https://doi.org/10.1016/j.envpol.2024.125475","url":null,"abstract":"2,4-bisphenol S (2,4-BPS) was an emerging BPS analogue as color developers, widely found in the environment. Fish toxicities, cytotoxicity and antiestrogenic effects of 2,4-BPS have been documented at mg L^-1, while the toxicity of 2,4-BPS at environmental concentrations (from ng L^-1 to μg L^-1) were scarce. Bacteria are identified as important components of the ecosystem, while little is known regarding the ecotoxicity of 2,4-BPS on bacteria. <em>Enterococcus faecalis</em>, a good indicator of faecal contamination and anthropogenic pollution, was exposed to 0.5-50 nmol L^-1 2,4-BPS. 2,4-BPS resulted in significantly decreased growth but notably increased membrane permeability in <em>E. faecalis</em> compared with the control. Hormetic effects on the expression of genes involved in DNA replication and efflux were observed. Inhibition of biofilm formation and induction of oxidative stress were caused by 0.5, 5 and 50 nmol L^-1 2,4-BPS. Fatty acyls, glycerolipids and glycerophospholipids were differentially regulated by 2,4-BPS. Glycerolipid metabolism and glycine, serine and threonine metabolism were significantly altered by 0.5 nmol L^-1 2,4-BPS, compared with glycerophospholipid metabolism disturbed by 5 and 50 nmol L^-1 2,4-BPS, showing concentration-dependent responses. Trend analysis of differential lipids demonstrated that there were three significant clusters, all of which were enriched in glycerophospholipid metabolism. 2,4-BPS elicited the strongest lipidomic responses at 5 nmol L^-1. Our study provides evidence for 2,4-BPS-induced toxicity to <em>E. faecalis</em> at environmental concentrations and contributes to a comprehensive understanding of the interaction between 2,4-BPS and Gram-positive bacteria.","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"122 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-compartment impact of micropollutants and particularly antibiotics on bacterial communities using environmental DNA at river basin-level","authors":"Pedro A. Inostroza, Gerdhard L. Jessen, Feilong Li, Xiaowei Zhang, Werner Brack, Thomas Backhaus","doi":"10.1016/j.envpol.2024.125487","DOIUrl":"https://doi.org/10.1016/j.envpol.2024.125487","url":null,"abstract":"Bacterial communities respond to environmental conditions with diverse structural and functional changes depending on their compartment (water, biofilm or sediment), type of environmental stress, and type of pollution to which they are exposed. In this study, we combined amplicon sequencing of bacterial 16S rRNA genes from water, biofilm, and sediment samples collected in the anthropogenically impacted River Aconcagua basin (Central Chile, South America), in order to evaluate whether micropollutants alter bacterial community structure and functioning based on the type and degree of chemical pollution. Furthermore, we evaluated the potential of bacterial communities from differently polluted sites to degrade contaminants. Our results show a lower diversity at sites impacted by agriculture and urban areas, featuring high loads of micropollution with pesticides, pharmaceuticals and personal care products as well as industrial chemicals. Nutrients, antibiotic stress, and micropollutant loads explain most of the variability in the sediment and biofilm bacterial community, showing a significant increase of bacterial groups known for their capabilities to degrade various organic pollutants, such as <em>Nitrospira</em> and also selecting for taxa known for antibiotic resistance such as <em>Exiguobacterium</em> and <em>Planomicrobium</em>. Moreover, potential ecological functions linked to the biodegradation of toxic chemicals at the basin level revealed significant reductions in ecosystem-related services in sites affected by agriculture and wastewater treatment plant (WWTP) discharges across all investigated environmental compartments. Finally, we suggest transitioning from simple concentration-based assessments of environmental pollution to more meaningful toxic pressure values, measured environmental concentrations normalised by effect information, in order to comprehensively evaluate the role of micropollutants at the ecological (biodiversity) level.","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"144 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142782859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of short and long-term cadmium exposure on behaviour and cardiac function in Drosophila.","authors":"Jessica J. Wellings, Jamie M. Thorpe, Karen Yendole, Yutaka Matsubayashi, Paul S. Hartley","doi":"10.1016/j.envpol.2024.125481","DOIUrl":"https://doi.org/10.1016/j.envpol.2024.125481","url":null,"abstract":"Cadmium is a non-essential heavy metal and environmental pollutant that causes a range of pathologies across different species. In humans, cadmium exposure has recently been directly linked to heart disease. Understanding how long-term cadmium exposure affects cardiac physiology is therefore important. In this work we employed a tractable <em>Drosophila melanogaster</em> model to study the effects of cadmium exposure on behaviour, lifespan and cardiac physiology. Dietary experiments established that cadmium at 10 μM and 100 μM was tolerated for several weeks, whereas doses in the mM range caused lethality within days. It was estimated that 10 μM dietary exposure represented an approximately 60-fold excess of the maximum exposure recommended for humans. Although 10 μM cadmium had no impact on lifespan compared to the control diet, it did cause significant daytime hyperactivity. Direct exposure of the heart to cadmium caused reversible cardiac arrest and disrupted calcium signalling. Compared to controls, 10 μM dietary cadmium had no impact on the rate of cardiac ageing over a six-week period. The higher dose of 100 μM shortened the flies’ lifespan but it slowed the rate of cardiac ageing. The findings indicate that <em>Drosophila</em> can be used to model the direct effects of cadmium on cardiomyocyte function and also demonstrate the existence of cardioprotective pathways triggered by dietary cadmium exposure. The data also indicate that cadmium at doses that do not affect lifespan or heart function, do cause daytime hyperactivity. Identifying and the cardioprotective mechanisms and understanding the hyperactivity phenotype in <em>Drosophila</em> may yield important findings of applied relevance to insects in general, as well as humans exposed to cadmium.","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"8 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142782857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}