Environmental Pollution最新文献

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Association of organophosphate flame retardants exposure with liver function and the contrasting mediating roles of inflammatory and oxidative stress pathways 有机磷阻燃剂暴露与肝功能的关系以及炎症和氧化应激途径的对比中介作用
IF 7.6 2区 环境科学与生态学
Environmental Pollution Pub Date : 2025-04-23 DOI: 10.1016/j.envpol.2025.126300
Yingyi Dai , Qin Li , Yongxian Li , Si Zhou , Xiaotong Li , Chongshan Guo , Jun Yuan , Lei Tan
{"title":"Association of organophosphate flame retardants exposure with liver function and the contrasting mediating roles of inflammatory and oxidative stress pathways","authors":"Yingyi Dai ,&nbsp;Qin Li ,&nbsp;Yongxian Li ,&nbsp;Si Zhou ,&nbsp;Xiaotong Li ,&nbsp;Chongshan Guo ,&nbsp;Jun Yuan ,&nbsp;Lei Tan","doi":"10.1016/j.envpol.2025.126300","DOIUrl":"10.1016/j.envpol.2025.126300","url":null,"abstract":"<div><div>Organophosphate flame retardants (OPFRs) have been widely used as alternatives to traditional halogenated flame retardants, resulting in their pervasive environmental presence and significant human exposure. While animal studies using high doses of individual OPFRs have demonstrated hepatotoxicity, evidence of hepatic effects from chronic and low-dose exposure to OPFRs in humans is scarce, and the underlying mechanisms remain largely unknown. In this study, we evaluated the association between urinary metabolites of OPFRs (mOPFRs) and liver function biomarkers in a population aged 1–79 years from the Guangzhou Human Biomonitoring Program. Urinary concentrations of diphenyl phosphate exhibited a significant decreasing trend with increasing age across four age groups (≤12, 13–17, 17–59, ≥60 years), while bis(1-chloro-2-propyl) phosphate concentrations showed a significant positive association with age. Urinary mOPFRs were positively associated with alanine aminotransferase, gamma-glutamyl transferase, and aspartate aminotransferase, and negatively associated with indirect bilirubin levels. Urinary mOPFRs were significantly correlated with total bilirubin, which was consistent with that observed in the National Health and Nutrition Examination Survey database, while other associations were inconsistent. Moreover, we evaluated the roles of inflammatory and oxidative stress markers in the association between urinary mOPFRs and liver function biomarkers by mediation analysis. The results demonstrated that the mediation effects of inflammatory markers were consistent with the total and direct effects of mOPFRs on liver function biomarkers, whereas the mediation effects of oxidative stress markers were discrepant. Inflammation likely exacerbates the adverse effects of OPFRs on liver function, while oxidative stress may play a protective role. We further searched the comparative toxicogenomics database for genes associated with OPFRs and liver function biomarkers to understand the potential regulatory mechanisms.</div></div>","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"375 ","pages":"Article 126300"},"PeriodicalIF":7.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143862815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Synergistic effects of surfactant biostimulation and indigenous fungal bioaugmentation for enhanced bioremediation of PAH-contaminated soils 表面活性剂生物刺激和本地真菌生物增强对多环芳烃污染土壤生物修复的协同效应
IF 7.6 2区 环境科学与生态学
Environmental Pollution Pub Date : 2025-04-23 DOI: 10.1016/j.envpol.2025.126304
Yeliang Dai , Xixi Cai , Shuang Wang , Chunxia Zhao , Xiyu Wang , Xiumin Yang , Xuan Zhao , Xianghui Cheng , Jibing Li , Chunling Luo , Gan Zhang
{"title":"Synergistic effects of surfactant biostimulation and indigenous fungal bioaugmentation for enhanced bioremediation of PAH-contaminated soils","authors":"Yeliang Dai ,&nbsp;Xixi Cai ,&nbsp;Shuang Wang ,&nbsp;Chunxia Zhao ,&nbsp;Xiyu Wang ,&nbsp;Xiumin Yang ,&nbsp;Xuan Zhao ,&nbsp;Xianghui Cheng ,&nbsp;Jibing Li ,&nbsp;Chunling Luo ,&nbsp;Gan Zhang","doi":"10.1016/j.envpol.2025.126304","DOIUrl":"10.1016/j.envpol.2025.126304","url":null,"abstract":"<div><div>Surfactant biostimulation and autochthonous fungal bioaugmentation have emerged as promising strategies for the bioremediation of soils contaminated with polycyclic aromatic hydrocarbons (PAHs). However, the mechanisms driving their combined effects remain poorly understood. This study investigates the degradation mechanisms associated with bioaugmentation using the indigenous fungus <em>Aspergillus fumigatus</em> LJD-29 and surfactant Tween 80. By employing stable-isotope probing and high-throughput sequencing, we comprehensively assessed these processes. In our study, the results demonstrate that both <em>Aspergillus fumigatus</em> LJD-29 and Tween 80 significantly enhanced the degradation efficiency of phenanthrene and modified the microbial community composition, particularly among active degraders. Extracellular enzymes were identified as key players in the phenanthrene transformation process. Tween 80 improved the bioavailability of phenanthrene, stimulating the growth of native PAH degraders, with <em>Pseudonocardia</em> emerging as a prominent genus. Although the combined surfactant-fungal treatment did not substantially increase terminal degradation efficiency due to limitations in phenanthrene bioavailability, it accelerated the degradation rate. Additionally, Tween 80 helped restore the microbial community structure disrupted by fungal bioaugmentation. These findings provide valuable insights into the mechanisms of surfactant biostimulation and indigenous fungal bioaugmentation, highlighting the potential of this integrated bioremediation strategy for managing PAH-contaminated soils.</div></div>","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"375 ","pages":"Article 126304"},"PeriodicalIF":7.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143862822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Precision management of Cd-contaminated paddy fields with high geochemical backgrounds in karst regions: integrating Bayesian decision tree and spatial zoning 喀斯特地区高地球化学背景镉污染稻田的精准管理:贝叶斯决策树与空间区划相结合
IF 7.6 2区 环境科学与生态学
Environmental Pollution Pub Date : 2025-04-22 DOI: 10.1016/j.envpol.2025.126282
Zhenyi Jia , Shenglu Zhou , Xuefeng Xie , Mingxing Xu , Qiuping Luo , Ting Zhu , Shaohua Wu
{"title":"Precision management of Cd-contaminated paddy fields with high geochemical backgrounds in karst regions: integrating Bayesian decision tree and spatial zoning","authors":"Zhenyi Jia ,&nbsp;Shenglu Zhou ,&nbsp;Xuefeng Xie ,&nbsp;Mingxing Xu ,&nbsp;Qiuping Luo ,&nbsp;Ting Zhu ,&nbsp;Shaohua Wu","doi":"10.1016/j.envpol.2025.126282","DOIUrl":"10.1016/j.envpol.2025.126282","url":null,"abstract":"<div><div>Cadmium (Cd) contamination in paddy fields with high geochemical backgrounds in karst regions poses significant challenges. This study aims to explore practical technologies applicable to different risk causal zones throughout karstic Cd-polluted paddy fields, by integrating a Bayesian decision tree-based risk warning model with spatial zoning. In the study area, 92.1 % of soil and 30.1 % of rice samples exceeded Cd limits, yet the synergy between elevated Cd levels in soil and rice was weak. The model achieved an accuracy of 93.4 % in predicting Cd risk in rice and generated 13 risk classification rules for evaluating Cd risk levels in paddy fields. Notably, the identified risk zones were primarily concentrated in areas with relatively low total Cd concentrations in the soil. Regional management framework, including passivation techniques, phytoremediation, agronomic regulation, was proposed according to 7 causal rules across the fields. Each zone was associated with a customized method for rice safe production, subsequently developing a spatially precise, differentiated solution. This study offers new insights into managing Cd-contaminated paddy fields in karst regions.</div></div>","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"375 ","pages":"Article 126282"},"PeriodicalIF":7.6,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigating the impact of fecal contamination on antibiotic resistance genes in urban environments using host-associated molecular indicators 利用宿主相关分子指标研究城市环境中粪便污染对抗生素耐药基因的影响
IF 7.6 2区 环境科学与生态学
Environmental Pollution Pub Date : 2025-04-22 DOI: 10.1016/j.envpol.2025.126296
Yujing Duan , Hui She , Lingna Jing , Lianrui Duan , Jinxiu Zheng , Ying Shao , Yuxin Che , Yu Shi , Chao Guo , Wenhui Zhao , Tao Yang , Lijun Yang
{"title":"Investigating the impact of fecal contamination on antibiotic resistance genes in urban environments using host-associated molecular indicators","authors":"Yujing Duan ,&nbsp;Hui She ,&nbsp;Lingna Jing ,&nbsp;Lianrui Duan ,&nbsp;Jinxiu Zheng ,&nbsp;Ying Shao ,&nbsp;Yuxin Che ,&nbsp;Yu Shi ,&nbsp;Chao Guo ,&nbsp;Wenhui Zhao ,&nbsp;Tao Yang ,&nbsp;Lijun Yang","doi":"10.1016/j.envpol.2025.126296","DOIUrl":"10.1016/j.envpol.2025.126296","url":null,"abstract":"<div><div>Antibiotic resistome of gut microbiota can be transmitted into the urban ecosystems via fecal pollution, potentially leading to a public health crisis. It is essential to determine the primary sources of fecal contamination and accurately evaluate the health risks for the propagation of antibiotic resistance genes (ARGs) from the intestinal microbiota. In this research, the occurrence, sources and potential hosts of ARGs in urban environmental samples collected from a wastewater treatment plant (WWTP), natural water bodies, tap water and farmland soil were comprehensively investigated. Host-associated fecal indicators for general warm-blooded animals (BacGeneral), humans (crAssphage), and other animals (bovines and swine) were employed for reliable microbial source tracking (MST). Results showed that the fecal indicator BacGeneral was detected in 84.00 % of collected environmental samples, indicating the widespread fecal contamination in local water and farmland. The WWTP was the reservoir and main source of fecal contamination in local environment, harboring the highest total abundances of ARGs (3.85 ± 2.72 ARGs/16S rRNA) and mobile genetic elements (MGEs) (0.32 ± 0.12 MGEs/16S rRNA) from multiple animals and humans. Although the swine-associated indicator was undetected, fecal contamination from both bovines and humans was prevalent in collected samples, with detection rates of pollution indicators at 52.00 % for bovine and 28.00 % for human sources. The co-occurrence of ARGs, fecal indicators and MGEs was analyzed, and significant correlation (<em>P</em> &lt; 0.01) between total ARG abundance and fecal indicator (BacGeneral) in contaminated environments demonstrated that fecal pollution exhibited a great influence on overall resistome in local environment. This research offers a comprehensive understanding of the sources and dissemination of ARGs in feces-polluted urban environments, providing data for the monitoring and prevention of ARG pollution.</div></div>","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"375 ","pages":"Article 126296"},"PeriodicalIF":7.6,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143862751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Integrating stable isotopes and hydrological models to track nitrogen sources and transport pathways in plateau watersheds: a case study in Southwest China 整合稳定同位素和水文模型追踪高原流域氮源和运移路径——以西南地区为例
IF 7.6 2区 环境科学与生态学
Environmental Pollution Pub Date : 2025-04-22 DOI: 10.1016/j.envpol.2025.126292
Qiyu Xu , Tiezhu Yan , Shufang Guo , Limei Zhai , Qiuliang Lei , Tianpeng Zhang , Xinzhong Du , Hongbin Liu
{"title":"Integrating stable isotopes and hydrological models to track nitrogen sources and transport pathways in plateau watersheds: a case study in Southwest China","authors":"Qiyu Xu ,&nbsp;Tiezhu Yan ,&nbsp;Shufang Guo ,&nbsp;Limei Zhai ,&nbsp;Qiuliang Lei ,&nbsp;Tianpeng Zhang ,&nbsp;Xinzhong Du ,&nbsp;Hongbin Liu","doi":"10.1016/j.envpol.2025.126292","DOIUrl":"10.1016/j.envpol.2025.126292","url":null,"abstract":"<div><div>Exogenous nitrogen inputs from agriculture and anthropogenic activities have dramatically altered the material cycling processes in the Plateau watershed, leading to a range of water pollution issues. Effective management of nitrogen pollution in water bodies is predicated on clarifying N export loads under different pathways in the watershed, as well as the contributions of different sources. Here, this study proposes an integrated framework that introduces multiple stable isotope techniques (δD-H<sub>2</sub>O, δ<sup>18</sup>O-H<sub>2</sub>O, δ<sup>15</sup>N-NO<sub>3</sub><sup>-</sup> and δ<sup>18</sup>O-NO<sub>3</sub><sup>-</sup>) based on the coupled Eckhardt's digital baseflow filter (ECK) and load estimation model (LOADEST). The integrated approach was applied for the first time in a typical plateau watershed in southwest China. Results showed that baseflow as the Fengyu River watershed (FRW) major hydrologic pathway, provides 69.6 % of the mean annual stream flow and 59.1 % of the mean annual NO<sub>3</sub><sup>−</sup>-N load. Furthermore, the FRW average annual TN and NO<sub>3</sub><sup>−</sup>-N export is 94.0 t and 55.1 t, respectively. The NO<sub>3</sub><sup>−</sup>-N was the primary form of N pollutant, with its average concentration in groundwater being 7 times that in river water. In groundwater, manure and sewage (M&amp;S) and soil nitrogen (SN) contribution rates to NO<sub>3</sub><sup>−</sup>-N 53.6 %, and 37.2 %, respectively. While the river water shows low M&amp;S (26.8 %) and high SN (61.5 %) characteristics. It can be seen that the baseflow is a key pathway for coupled water-nitrogen export from plateau agricultural watersheds. Blocking the migration of nitrogen-containing pollutants to groundwater is an important measure to control the degradation of the water environment in plateau watersheds from the root cause.</div></div>","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"374 ","pages":"Article 126292"},"PeriodicalIF":7.6,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Heavy metals prediction system in groundwater using online sensor and machine learning for water management: the case of typical industrial park 基于在线传感器和机器学习的地下水重金属预测系统——以典型工业园区为例
IF 7.6 2区 环境科学与生态学
Environmental Pollution Pub Date : 2025-04-22 DOI: 10.1016/j.envpol.2025.126270
Junfan Zhang , Yuzhi Xuan , Jingjing Lei , Liping Bai , Guobang Zhou , Yuelong Mao , Peinian Gong , Menghuan Zhang , Dajian Pan
{"title":"Heavy metals prediction system in groundwater using online sensor and machine learning for water management: the case of typical industrial park","authors":"Junfan Zhang ,&nbsp;Yuzhi Xuan ,&nbsp;Jingjing Lei ,&nbsp;Liping Bai ,&nbsp;Guobang Zhou ,&nbsp;Yuelong Mao ,&nbsp;Peinian Gong ,&nbsp;Menghuan Zhang ,&nbsp;Dajian Pan","doi":"10.1016/j.envpol.2025.126270","DOIUrl":"10.1016/j.envpol.2025.126270","url":null,"abstract":"<div><div>With the expansion of human industrial activities, heavy metal contamination in groundwater environments has become increasingly severe. Environmental management agencies invest significant financial resources into groundwater monitoring, primarily due to its inherent invisibility. Automatic monitoring is a new way to monitor groundwater, the existing sensors often can only achieve simple indicators, and it is difficult to achieve complex indicators such as heavy metals. This study integrated pH and conductivity online monitoring probes with machine learning algorithms to develop a real-time, automated heavy metal prediction system for groundwater. The predictive performance demonstrated that the highest R<sup>2</sup> values for chromium (Cr), nickel (Ni), and copper (Cu) were 0.73, 0.78, and 0.87, respectively, with mean absolute errors of 11.9, 0.83, and 1.02 μg/L. While random forest and extreme gradient boosting (XGB) models demonstrate greater robustness. To enhance the practicality and management significance of the prediction system, interval prediction is employed. Uncertainty assessment results indicate that the performance order of prediction intervals across different models is XGB &gt; Random Forest &gt; Multiple Linear Regression (MLR) &gt; Backpropagation neural network (BP). We proposed that Groundwater risk is acceptable when the prediction interval of pollutants falls below regional screening levels. The integration of automated sensors with machine learning algorithms can offer advanced recommendations for long-term environmental monitoring.</div></div>","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"374 ","pages":"Article 126270"},"PeriodicalIF":7.6,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Synergistic enhancement of hydrophobic n-hexane biodegradation in a biotrickling filter: Role of hydrophilic VOCs in mass transfer and extracellular polymeric secretion 生物滴滤中疏水正己烷生物降解的协同增强:亲水VOCs在传质和细胞外聚合物分泌中的作用
IF 7.6 2区 环境科学与生态学
Environmental Pollution Pub Date : 2025-04-22 DOI: 10.1016/j.envpol.2025.126297
Zeyu Wang , Lingxiu Liu , Hongye Fan , Dongyun Ye , Dzmitry Hrynsphan , Savitskaya Tatsiana , Xiaole Weng , Jun Chen
{"title":"Synergistic enhancement of hydrophobic n-hexane biodegradation in a biotrickling filter: Role of hydrophilic VOCs in mass transfer and extracellular polymeric secretion","authors":"Zeyu Wang ,&nbsp;Lingxiu Liu ,&nbsp;Hongye Fan ,&nbsp;Dongyun Ye ,&nbsp;Dzmitry Hrynsphan ,&nbsp;Savitskaya Tatsiana ,&nbsp;Xiaole Weng ,&nbsp;Jun Chen","doi":"10.1016/j.envpol.2025.126297","DOIUrl":"10.1016/j.envpol.2025.126297","url":null,"abstract":"<div><div>Volatile organic compounds (VOCs) emitted from the pharmaceutical industry, such as <em>n</em>-hexane, dichloromethane (DCM), and <em>N</em>, <em>N</em>-dimethylacetamide (DMAC), pose significant environmental and health risks. This study evaluates the performance of a bamboo charcoal-based bio-trickling filter (BTF) in removing these VOCs, comparing two systems: BTF-a, treating only <em>n</em>-hexane, and BTF-b, treating a mixture of <em>n</em>-hexane, DCM, and DMAC. After 26 days of operation at an <em>n</em>-hexane concentration of 100 mg m<sup>−3</sup>, BTF-b achieved a removal efficiency (RE) of 87.25 % for <em>n</em>-hexane, significantly higher than the 62 % in BTF-a, showcasing the synergistic effects of multi-component VOCs. As the DMAC concentration increased from 100 mg L<sup>−1</sup> to 500 mg L<sup>−1</sup>, the RE of <em>n</em>-hexane improved from 65.93 % to 82.08 %, with a significant enhancement in the mass transfer coefficient (K<sub>L</sub>a) from 1.75 × 10<sup>−6</sup> to 6.34 × 1<sup>−5</sup> s<sup>−1</sup>, demonstrating the positive promotional effect of DMAC on <em>n</em>-hexane biodegradation. Conversely, high DCM concentrations (750 mg m<sup>−3</sup>) reduced the RE of <em>n</em>-hexane to 58.57 % due to substrate competition. The extracellular proteins (PN) content rose from 16.76 mg g<sup>−1</sup> to 18.73 mg g<sup>−1</sup>. Microbial analysis after 220d operation revealed the enrichment of <em>Mycobacterium</em> (57.67 %) and <em>Hyphomicrobium</em> (5.38 %), key genera involved in VOCs biodegradation. Metabolic pathway analysis showed <em>n-</em>hexane and DMAC were transformed into intermediates, including hexanoic acid and acetic acid, demonstrating both pollutant removal and resource recovery potential. These findings highlight the synergistic role of hydrophilic VOCs, which are more hydrophilic than <em>n</em>-hexane, in promoting the biodegradation of hydrophobic pollutants, as well as the feasibility of BTF for treating multi-component VOCs.</div></div>","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"375 ","pages":"Article 126297"},"PeriodicalIF":7.6,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143862823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
6-plex Crystal Digital PCR® for comprehensive surveillance of respiratory and foodborne bacterial pathogens in wastewater 6-plex Crystal Digital PCR® 用于全面监测废水中的呼吸道和食源性细菌病原体
IF 7.6 2区 环境科学与生态学
Environmental Pollution Pub Date : 2025-04-22 DOI: 10.1016/j.envpol.2025.126298
Sadhana Shrestha, Bikash Malla, Eiji Haramoto
{"title":"6-plex Crystal Digital PCR® for comprehensive surveillance of respiratory and foodborne bacterial pathogens in wastewater","authors":"Sadhana Shrestha,&nbsp;Bikash Malla,&nbsp;Eiji Haramoto","doi":"10.1016/j.envpol.2025.126298","DOIUrl":"10.1016/j.envpol.2025.126298","url":null,"abstract":"<div><div>Bacterial wastewater surveillance (WS) is less explored area compared to viral WS despite high burden of bacterial respiratory and gastrointestinal infections worldwide. This study established a 6-plex Crystal Digital PCR® (cdPCR) system, to comprehensively monitor an acute respiratory pathogen - Group A <em>Streptococcus</em> (GAS) <em>pyogenes</em>, foodborne disease (FBD) pathogens - <em>Clostridium perfringens</em>, <em>Salmonella</em> spp., <em>Campylobacter jejuni,</em> and <em>Campylobacter coli</em>, and an indicator bacterium, <em>Escherichia coli</em> in wastewater. Fifty-two grab influent samples collected weekly from a wastewater treatment plant in Yamanashi Prefecture, Japan, between June 2023 and May 2024 were centrifuged, followed by DNA extraction and cdPCR. cdPCR was performed using the naica® system (Stilla Technologies). The 6-plex cdPCR assays showed strong performance. Among the 52 samples, 100 % of samples were positive for <em>C. perfringens</em>, 98 % for <em>Salmonella</em> spp., 56 % for <em>C. jejuni</em>, 25 % for <em>C. coli,</em> and 63 % for <em>S. pyogenes,</em> with concentrations ranging between 4.2 ± 0.3 to 7.5 ± 0.2 log<sub>10</sub> copies/L. The concentration of <em>C. perfringens</em> was significantly higher than that of other pathogens (<em>p</em> &lt; 0.05), indicating its dominance. <em>Salmonella</em> spp. had high detection rate, implying increased <em>Salmonella</em> infection in the population. Seasonal variation was not observed in any of FBD pathogens, except for the detection rate of <em>C. coli</em>. <em>S. pyogenes</em> concentrations were significantly higher in spring than in other seasons, agreeing with the trend of GAS pharyngitis cases in the catchment. In conclusion, the 6-plex cdPCR system is a valuable tool for comprehensive WS, offering significant implications for public health monitoring.</div></div>","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"375 ","pages":"Article 126298"},"PeriodicalIF":7.6,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143862821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fluorinated liquid-crystal monomers distribution in paired urine from mothers and infants 含氟液晶单体在母婴配对尿液中的分布
IF 7.6 2区 环境科学与生态学
Environmental Pollution Pub Date : 2025-04-21 DOI: 10.1016/j.envpol.2025.126283
Runhui Yang , Yanli Zhang , Qun Gao , Chenhui Sang , Yumin Niu , Shiyu Du , Bing Shao
{"title":"Fluorinated liquid-crystal monomers distribution in paired urine from mothers and infants","authors":"Runhui Yang ,&nbsp;Yanli Zhang ,&nbsp;Qun Gao ,&nbsp;Chenhui Sang ,&nbsp;Yumin Niu ,&nbsp;Shiyu Du ,&nbsp;Bing Shao","doi":"10.1016/j.envpol.2025.126283","DOIUrl":"10.1016/j.envpol.2025.126283","url":null,"abstract":"<div><div>Fluorinated liquid-crystal monomers (FLCMs) are widespread environmental contaminants with potential endocrine-disrupting effects. Infants are particularly vulnerable, yet their exposure remains unclear. This study analyzed FLCMs in urine samples from 190 paired mothers and infants in Beijing, detecting 34 and 35 FLCMs, respectively. Median creatinine-corrected concentrations were 1.83 μg/g (unadjusted concentrations: 1.28 ng/mL) for mothers and 3.28 μg/g (0.60 ng/mL) for infants. 1-butoxy-2,3-difluoro-4-(trans-4-propylcyclohexyl) benzene (BDPrB) and 1-ethyl-4-[(4-fluorophenyl) ethynyl] benzene (EFPEB) were identified as the primary detected contaminants. A significant positive correlation in urine concentrations between mothers and infants was observed only for 2′-Fluoro-4″-propyl-[1,1':4′,1″-terphenyl]-4-carbonitrile (FPTC) (r<sub>s</sub> = 0.23, <em>p</em> = 0.023). Certain FLCMs were associated with infant feeding patterns, maternal parity, and environmental exposure, including dust and cleaning frequency (<em>p</em> &lt; 0.05). The results of the study showed that the median estimated daily intakes (EDIs) of ∑FLCMs for mothers and infants were 526 and 425 ng/kg bw/day, respectively, with no significant difference between them (<em>p</em> &gt; 0.05). Further stratification of the data by sex revealed that for male infants, the EDI values for BDPrB and EFPEB were greater (<em>p</em> &lt; 0.05). These findings emphasize the need for greater research on the health effects of FLCMs on infants, particularly considering gender differences.</div></div>","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"375 ","pages":"Article 126283"},"PeriodicalIF":7.6,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Climate change, mercury pollution, and global ecology 气候变化、汞污染和全球生态学
IF 7.6 2区 环境科学与生态学
Environmental Pollution Pub Date : 2025-04-21 DOI: 10.1016/j.envpol.2025.126284
Michael S. Bank , Zoyne Pedrero Zayas , Vernon Somerset , Lynwill G. Martin , Milena Horvat
{"title":"Climate change, mercury pollution, and global ecology","authors":"Michael S. Bank ,&nbsp;Zoyne Pedrero Zayas ,&nbsp;Vernon Somerset ,&nbsp;Lynwill G. Martin ,&nbsp;Milena Horvat","doi":"10.1016/j.envpol.2025.126284","DOIUrl":"10.1016/j.envpol.2025.126284","url":null,"abstract":"","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"375 ","pages":"Article 126284"},"PeriodicalIF":7.6,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143857853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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