Environmental Pollution最新文献

{"title":"Transfer of prosulfocarb and boscalid residues from maize leaves to soil and their effects on soil microorganisms","authors":"Philipp Mäder ,&nbsp;Anja Listl ,&nbsp;Zuzana Hochmanová ,&nbsp;Wolfgang Armbruster ,&nbsp;Paula Harkes ,&nbsp;Christian Poll ,&nbsp;Ellen Kandeler","doi":"10.1016/j.envpol.2025.126862","DOIUrl":"10.1016/j.envpol.2025.126862","url":null,"abstract":"<div><div>Plant materials that have been in contact with pesticides can be incorporated into the soil, posing a potential risk to non-target soil organisms and, hence, soil functions. This study investigated effects of two pesticides applied to maize leaves on the soil microbial community, activity and function. The herbicide prosulfocarb (PSC) and the fungicide boscalid (BSC) were applied alone or in combination to fresh or aged maize leaves, which were incorporated into soil. During a 56-day incubation we quantified pesticide residues in soil and maize leaves as well as maize-derived C incorporation into different microbial fractions (CO₂, extractable organic carbon, microbial biomass and main microbial groups). Prosulfocarb residues on maize and in soil decreased to below 5 % after 56 days. However, BSC residues were transferred from maize into the soil, as indicated by an increase in BSC residues in soil of around 15 %. Prosulfocarb initially inhibited the synthesis of soil bacterial phospholipids by 25–45 %, which was accompanied by a decrease in the incorporation of maize-derived C into microbial biomass by 68–70 %. Following this, microorganisms shifted their nutrient acquisition strategy towards carbon and phosphorus, which led to increased utilization of easily available maize-derived C. Boscalid transiently inhibited the growth of soil fungi, reduced soil respiration, and mineralization of maize. In the future, pesticide accumulation through transfer from plant material into soils and the mode of action dependent effects on soil microorganisms need to be considered for risk assessment.</div></div>","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"383 ","pages":"Article 126862"},"PeriodicalIF":7.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal Bioaccumulation and Biomagnification of Typical Organochlorine Pesticides in the Pearl River Estuary, South China","authors":"Xutong Zhang, Yiye Jiang, Yanhong Zeng, Jian Guo, Ling Long, Zijian Pan, Yankuan Tian, Yanting Zhang, Xiaojun Luo, Pingan Peng, Bixian Mai","doi":"10.1016/j.envpol.2025.126855","DOIUrl":"https://doi.org/10.1016/j.envpol.2025.126855","url":null,"abstract":"As a typical organochlorine pesticide, dichlorodiphenyltrichloroethane (DDT) and its metabolites (dichlorodiphenyldichloroethane (DDD), dichlorodiphenylmethane (DDM), dichlorodiphenyldichloroethylene (DDE), and bis(p-chlorophenyl)-chloroethylene (DDMU)) (collectively referred to as DDXs), have been widely detected in the Pearl River Estuary (PRE). However, seasonal variations in land runoff and the transformation of DDXs complicate their bioaccumulation characteristics in the PRE. The present study analyzed the sediment and aquatic organisms from both dry and wet seasons to investigate the seasonal characteristics of bioaccumulation of DDXs in the PRE. The median concentration of DDXs in sediments was significantly higher during the wet season (0.60 ng/g dw) than that in the dry season (0.25 ng/g dw), and the median concentration of DDXs in aquatic organisms was significantly higher during the dry season (518 ng/g lw) than that in the wet season (189 ng/g lw). Based on δ¹³C and δ¹⁵N values, the aquatic food web is composed of terrestrial and marine food chains. Using an improved trophic position (TP) quantification model (TP_{bulk-correction}), significantly different correlation trends of DDXs in the food chain during the dry season were observed: along with the increasing of TPs, the abundance of DDT and DDM+DDMU decreased, while the abundance of DDD+DDE increased, which likely due to the DDX biotransformation. Additionally, this study found that elevated terrestrial nutrient input and the maternal-offspring transfer of DDXs could conceal the DDX bioaccumulation process during the wet season. Compared to the traditional TP quantification model (TP_bulk), the TP_{bulk-correction} model, which differentiates terrestrial from marine sources, can effectively reveal the significant correlation between the DDX composition and TPs. This emphasizes the need to integrate environmental conditions and biological processes for a comprehensive analysis of the biological behavior of pollutants.","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"10 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144684697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Herbicide formulations impair in vitro performance of hazel pollen: natural is not safer.","authors":"Davide Gerna, Fabio Candotto Carniel, Louise Colville, Johanna Wagner, Daniela Festi","doi":"10.1016/j.envpol.2025.126870","DOIUrl":"https://doi.org/10.1016/j.envpol.2025.126870","url":null,"abstract":"The progamic phase from pollination to fertilisation is a critical stage of plant life cycle that is vulnerable to chemical exposure and requires controlled production of reactive oxygen species (ROS). Herbicide formulations are integral to agriculture, horticulture, and forestry, though surprisingly little is known about pollen exposure to commercial herbicide formulations, especially for non-target (i.e., non-weed) plants. Here, we compared the effects of a synthetic formulation of glyphosate (Roundup) and a natural formulation of pelargonic acid (Herbistop) on the <em>in vitro</em> performance of hazel (<em>Corylus avellana</em> L.) pollen. At equal pH values, Herbistop (half-maximal inhibitory concentration [IC50] 10.7-11.2 mg acid equivalents [a.e.] L^-1) restricted pollen tube elongation at concentrations one order of magnitude lower than Roundup (IC50 332.0-410.0 mg a.e. L^-1) and in a narrower range. Pollen staining with triphenyl tetrazolium chloride confirmed the toxicity of both formulations, with Herbistop compromising viability more severely. At respective IC50 values, both herbicide formulations curtailed the production of the ROS hydrogen peroxide during pollen tube elongation, impairing viability, and slowing down <em>in vitro</em> germination. Overall, our <em>in vitro</em> evidence shows that the natural formulation, Herbistop, was more hazardous for hazel pollen performance than the synthetic formulation, RoundUp. This highlights the common misconception that usage of natural herbicide formulations, rather than synthetic, is safer for non-target plants, other non-target organisms, and the environment. We propose that pollen performance is an additional toxicological endpoint of all herbicide formulations that requires special surveillance in gardens, woodlands, and agroecosystems.","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"25 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of earthworm coelomic fluid inhibits multidrug-resistant bacteria and blocks resistance transmission","authors":"Lihan Lin, Jingwen Luo, Zhan Li, Hongan Guo, Tao Liu, Xiaojie Sun, Meiyan Xing","doi":"10.1016/j.envpol.2025.126817","DOIUrl":"https://doi.org/10.1016/j.envpol.2025.126817","url":null,"abstract":"Antibiotic resistance is a growing global health crisis, especially the spread of multi-drug resistance. In this study, the inhibitory effects of earthworm coelomic fluid (ECF) on multidrug-resistant bacteria (MRB) were investigated during employing vermicomposting to treat excess sludge generated from wastewater treatment. The results demonstrated that the ECF was able to inhibit, even completely decompose the MRB. Notably, when the ECF concentration reached 1.0 mg/mL, the intracellular reactive oxygen species (ROS) level increased by 46.7%, while cell viability decreased by 55.2% compared to the control, demonstrating that ECF exerts strong antibacterial activity by inducing oxidative stress and disrupting cellular homeostasis. Furthermore, ECF effectively degraded the DNA of MRB, with removal rates of aphA, KanR, and tetA reaching 51.8%, 42.3%, and 35.0%, respectively, indicating its ability to eliminate resistance genes and hinder their potential transfer. Additionally, the upregulation of genes involved in signaling, DNA replication and repair, and energy metabolism pathways suggests a systemic stress response in MRB, further supporting the broad-spectrum inhibitory effects of ECF on bacterial viability and resistance maintenance.. Taken together, these findings may open a door to naturally and ecologically combat antibiotic resistance in pollutants control in wastewater treatment.","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"106 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144684701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microplastics transport and impact on nitrogen cycling and N2O emissions in estuaries","authors":"Muhammad Ayaz ,&nbsp;Yoong-Sin Oon ,&nbsp;Yoong-Ling Oon ,&nbsp;Kifayatullah Khan ,&nbsp;Min Deng ,&nbsp;Lu Li ,&nbsp;Kang Song ,&nbsp;Xiaoyan Jiang ,&nbsp;Zhiwei Xia","doi":"10.1016/j.envpol.2025.126869","DOIUrl":"10.1016/j.envpol.2025.126869","url":null,"abstract":"<div><div>Microplastic pollution in estuarine ecosystems disrupts nitrogen cycling and enhances nitrous oxide (N₂O) emissions, reinforcing the role of estuaries as greenhouse gas (GHG) hotspots. This review integrates mechanisms that modulate microplastic-induced disruptions to nitrogen cycling processes and transform estuarine biogeochemistry. It elucidates key mechanistic pathways whereby microplastic dynamics influence microbial nitrogen transformations and alter GHG fluxes. Microplastics affect nitrogen cycling through multiple mechanisms, including adsorption of nitrogenous compounds, restructuring of microbial communities, and modulation of enzymatic processes that control nitrogen transformations. Within plastisphere biofilms, microplastics foster microbial interactions that promote incomplete denitrification and nitrifier-driven N₂O production, intensifying N₂O fluxes from estuarine sediments and waters. The review synthesizes recent findings on microplastic degradation, genetic drift, and horizontal gene transfer, which may further reshape nitrogen cycling capacity over time. Recent advancements in microplastic characterization, including aptamer-based sensors, flow cytometry, and improved extraction methods, enhance the ability to quantify and trace microplastic impacts in estuarine environments. This review proposes an integrative conceptual model for microplastic-mediated amplification of N₂O emissions in estuaries and identifies critical research and policy directions. Addressing microplastic-induced disruptions of nitrogen cycling and GHG dynamics will require integrated mitigation strategies, targeted regulatory interventions, and interdisciplinary research to support sustainable estuarine management.</div></div>","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"383 ","pages":"Article 126869"},"PeriodicalIF":7.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144684700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reversal of neurodevelopmental toxicity induced by combined exposure to the neonicotinoid insecticides acetamiprid and imidacloprid in zebrafish through targeting DRP-1: insights into mitophagy and apoptosis mechanisms","authors":"Yuzhi Zhao, Shaozhuo Wang, Siyue Tan, Gaoyuan Wang, Haojie Zhou, Chengyu Geng, Chunjin Li, Yuewen He, Yifan Shi, Zhongxiu Deng, Siyu Chen, Qitong Yuan, Sirui Wang, Yuxi Yang, Xinyan Jiang, Wenqing He, Shou-Lin Wang, Huibin Dong, Chao Wang","doi":"10.1016/j.envpol.2025.126877","DOIUrl":"https://doi.org/10.1016/j.envpol.2025.126877","url":null,"abstract":"Neonicotinoid pesticides, including acetamiprid (ACE) and imidacloprid (IMI), are widely used due to their high efficacy and broad-spectrum activity. Despite IMI prohibition in certain countries by 2023, its residual effects, particularly in conjunction with ACE, which is frequently detected at elevated concentrations in rivers and surface waters, remain inadequately explored. In this study, the combined neurodevelopmental toxicity of ACE and IMI in zebrafish was investigated, with emphasis on mitochondrial dynamics and potential intervention strategies. We developed a 120-h acute exposure model using zebrafish embryos and a long-term chronic exposure model involving six months of environmentally relevant doses in adult zebrafish. Various parameters were systematically assessed using these models, including general toxicity, larval behavioral characteristics, outcomes of the novel tank diving test and light-dark test in adults, neurodevelopmental status, associated gene expression levels, and mitochondrial function. Furthermore, exposure experiments were conducted using transgenic zebrafish larvae <em>Tg</em> (<em>huc:eGFP</em>) and <em>Tg</em> (<em>hb9:eGFP</em>) to elucidate specific effects on the nervous system. Human neuroblastoma SK-N-SH cells were used to evaluate apoptosis, oxidative stress, ATP levels, mitochondrial membrane potential, and calcium ion concentrations. Protein markers associated with apoptosis (Bax, BCL-2, and Cleaved Caspase-3) and mitophagy (LC3A/B, P62, Parkin, and Pink-1) were analyzed. Additionally, DRP-1, Bax, and CytC levels were quantified in both mitochondrial and cytoplasmic fractions. The DRP-1 inhibitor Mdivi-1 was used to substantiate the role of mitochondrial dynamics. The results revealed a synergistic neurotoxic effect resulting from the combined exposure to ACE and IMI, which was characterized by impaired neural development, behavioral abnormalities, and mitochondrial dysfunction. Treatment with Mdivi-1 ameliorated these effects, reducing neurotoxicity in zebrafish. This study elucidates the synergistic neurodevelopmental toxicity of ACE and IMI, underscores the pivotal role of mitochondrial pathways, and provides insights into potential mitigation strategies for neonicotinoid-induced neurotoxicity.","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"16 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144684698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioaccumulation of Trace Elements in White Storks (Ciconia ciconia): Effects of Age, Health, and Anthropogenic Exposure","authors":"Rocío Fernández-Valeriano, Natalia Pastor Tiburón, Fernando González, Norberto Ruiz-Suárez, Manuel Zumbado, Beatriz Martín-Cruz, Ángel Rodríguez-Hernández, Andrea Acosta-Dacal, Luis Alberto Henríquez-Hernández, Octavio P. Luzardo","doi":"10.1016/j.envpol.2025.126852","DOIUrl":"https://doi.org/10.1016/j.envpol.2025.126852","url":null,"abstract":"White storks (<em>Ciconia ciconia</em>) are recognized as effective bioindicators of environmental contamination due to their wide distribution and trophic flexibility. In this study, we analyzed blood concentrations of 47 essential, toxic, and potentially toxic elements in 189 white storks from central Spain, assessing the influence of age, health status, and anthropogenic pressure on metal accumulation. Birds were grouped into chicks, fledglings, and adults. Statistical comparisons were performed using non-parametric tests and general linear models (GLMs), depending on data distribution. Our findings indicate that age significantly affects metal accumulation, with fledglings exhibiting higher concentrations of lead (Pb, p = 0.0024), arsenic (As, p = 0.0012), cadmium (Cd, p = 0.0476), and manganese (Mn, p = 0.0467) compared to adults, suggesting increased exposure through parental feeding and trophic transfer. Health status was also a critical determinant: sick individuals showed significantly elevated levels of Cd (p &lt; 0.0001), Pb (p &lt; 0.0001), and As (p = 0.0166), supporting the role of metal toxicity in avian morbidity. In terms of anthropogenic exposure, storks sampled within 30 km of landfills exhibited significantly higher concentrations of As (p = 0.0002), Cd (p = 0.0118), and Hg (p = 0.0412). Individuals with foreign materials in the digestive tract also showed increased Pb (p = 0.0007) and Cd (p = 0.0008) levels. Conversely, no significant differences were found between individuals from areas of high versus low human population density. These results highlight the impact of environmental pollution on metal bioaccumulation in white storks and demonstrate the influence of landfill proximity and trophic exposure on contaminant burdens. Given their role as a sentinel species, our findings underscore the need for stricter waste management policies and continued biomonitoring efforts to mitigate toxic metal exposure in wildlife.","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"24 1","pages":""},"PeriodicalIF":8.9,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prioritization of legacy and emerging halogenated organic contaminants in humpback dolphins from northern South China Sea","authors":"Zhenhui Xie ,&nbsp;Qiang Xie ,&nbsp;Bin Sun ,&nbsp;Yanqing Xie ,&nbsp;Fei Liu ,&nbsp;Yanling Gan ,&nbsp;Jiaxue Wu ,&nbsp;Xiyang Zhang ,&nbsp;Yuping Wu","doi":"10.1016/j.envpol.2025.126864","DOIUrl":"10.1016/j.envpol.2025.126864","url":null,"abstract":"<div><div>Marine mammals are prone to accumulating elevated levels of halogenated organic contaminants (HOCs) due to their long lifespans, high trophic positions, and substantial lipid stores. However, which HOCs are of greatest concern in these apex predators remain largely unknown. Here, we constructed a toxicological prioritization index (ToxPi) model that integrates detection frequency, persistence, bioaccumulation, and endocrine-disrupting potential to evaluate priority HOCs in Indo-Pacific humpback dolphins (<em>Sousa chinensis</em>, n = 70) from the northern South China Sea (NSCS) between 2008 and 2018. Our findings revealed that legacy HOCs—particularly dichlorodiphenyltrichloroethanes (DDTs) and hexachlorocyclohexanes (HCHs)—ranked as the highest priority HOCs, highlighting their long-lasting health risk to the dolphins despite the global prohibition of these chemicals. Notably, although alternative halogenated flame retardants (AHFRs), as emerging contaminants, constituted the least proportion (0.03 %) among the HOCs analyzed, their ToxPi scores (0.38–0.44) were just below those of DDTs (0.46–0.53) and HCHs (0.48–0.50). This suggests that AHFRs may not be suitable substitutes for traditional flame retardants, and their potential ecological risks deserve further attention. Significant correlations were observed between hormones (thyroid hormones and testosterone) and high-scoring congeners within each HOC category. It is further supported that these priority HOCs may cause endocrine disorders in humpback dolphins through the actual measurement data. Overall, this study identified a priority list of HOCs in the NSCS humpback dolphins, emphasizing the need to monitor both legacy and emerging HOCs in marine mammals, without favoring one over the other.</div></div>","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"383 ","pages":"Article 126864"},"PeriodicalIF":7.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microplastics in the Euphrates: Assessing contamination in water, sediment, and commercial fish species","authors":"Abdullah Altunışık ,&nbsp;Hale Tatlı ,&nbsp;Necmettin Doğan ,&nbsp;Göksal Sezen ,&nbsp;Arif Parmaksız","doi":"10.1016/j.envpol.2025.126863","DOIUrl":"10.1016/j.envpol.2025.126863","url":null,"abstract":"<div><div>Microplastics (MPs) are increasingly recognized as emerging pollutants in freshwater ecosystems, with implications for ecological integrity and food safety. This study presents the first integrated assessment of MP contamination in the Euphrates River (Türkiye), focusing on water, sediment, and six commercially important fish species. We hypothesized that MP abundance in fish would vary according to species-specific feeding behavior and habitat use. To test this, sampling was conducted at 23 sites encompassing urban, agricultural, and industrial zones. MPs were identified and quantified using stereomicroscopy and Fourier-transform infrared spectroscopy. MPs were detected in 52.2 % of water samples (0–6.6 MP/m³) and 43.5 % of sediment samples (0–15 MP/kg), with fibers and polyethylene terephthalate were the dominant forms. Among fish species (<em>Capoeta trutta</em>, <em>Carasobarbus luteus</em>, <em>Cyprinus carpio</em>, <em>Cyprinion macrostomus</em>, <em>Chondrostoma regium</em>, and <em>Carassius gibelio</em>), MP abundance ranged from 0.2 to 1.76 MPs/individual. Contrary to our initial hypothesis, MP loads did not significantly differ among species or locations. Moreover, statistical analyses revealed no significant associations between MP ingestion and fish traits such as age, length, or weight. However, relatively high MP levels were observed in <em>C. trutta</em>, possibly reflecting its benthopelagic habitat and omnivorous diet. These findings point to a diffuse and widespread pattern of MP contamination, likely driven by multiple anthropogenic sources and hydrodynamic factors. The study underscores the need for targeted pollution control strategies and highlights the ecological vulnerability of the Euphrates River.</div></div>","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"383 ","pages":"Article 126863"},"PeriodicalIF":7.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Steroid hormones and their combinations affect circadian rhythm pathway in F0 Gambusia affinis using transcriptome sequencing: validation by life-long exposure of F1 offspring and molecular dynamics","authors":"Guo-Yong Huang ,&nbsp;Gui-Zhen Fang ,&nbsp;Shi-Quan Zou ,&nbsp;Guang-Guo Ying","doi":"10.1016/j.envpol.2025.126866","DOIUrl":"10.1016/j.envpol.2025.126866","url":null,"abstract":"<div><div>Estrogens and androgens are widely distributed in aquatic environments, yet the molecular mechanisms underlying their potential interactive effects in fish remain poorly understood. This study assessed the hepatic effects of 17α-ethinylestradiol (EE2), 17α-methyltestosterone (MT) at low (MTL) and high (MTH) concentrations, and their mixtures (EE2+MTL and EE2+MTH) in two generations of <em>Gambusia affinis</em>. RNA sequencing revealed treatment-dependent transcriptional changes in the liver of F0 female, with more unique than overlapping differentially expressed genes across treatments. Both individual and combined EE2 and MT exposure disrupted circadian rhythm-related pathways in F0 female. Moreover, long-life exposure of F1 offspring to EE2, MT, and their mixtures significantly altered the transcriptional levels of circadian rhythm-related genes (e.g., <em>nr1d1</em>) in the liver. The precise tertiary structure of <em>G. affinis</em> NR1D1 was constructed, and molecular docking demonstrated that EE2, MT, and their complexes bind to NR1D1. Molecular dynamics simulations revealed that the binding free energies of EE2 and MT complexes with NR1D1 were lower than those of EE2 or MT alone. These findings suggest that both hormones and their mixtures have significant potential to disrupt circadian rhythm in <em>G. affinis</em>.</div></div>","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"383 ","pages":"Article 126866"},"PeriodicalIF":7.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144677713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}