National Journal of Laboratory Medicine最新文献

{"title":"Prevalence of Anaemia and Thrombocytopenia in Pregnant Females in Amreli District, Gujarat","authors":"P. Kagathara, Abhishek Godhani, Keval Rajeshkumar Pandya","doi":"10.7860/njlm/2022/49637.2565","DOIUrl":"https://doi.org/10.7860/njlm/2022/49637.2565","url":null,"abstract":"Introduction: Anaemia in pregnancy is a major public health problem in India. It leads to high maternal morbidity and mortality, low birth weight and high infant mortality. Thrombocytopenia can also affect the pregnancy state and along with preeclampsia, there is a high risk of complications to both mother and baby. So, it should be aimed to keep in normal range. With early intervention, maternal morbidity and mortality as well as infant mortality can be reduced. Aim: To assess the prevalence of anaemia and thrombocytopenia in pregnant females. Materials and Methods: This retrospective study was conducted to evaluate the prevalence of anaemia and thrombocytopenia in pregnant females. Blood samples of 500 pregnant females of Shantabaa Medical College and General Hospital, Gujarat, India were evaluated to assess the frequency of anaemia and thrombocytopenia using haematology analyser. It was hospital-based study done for six months, from August 2020 to January 2021. All these females were divided into mild (10-10.9 gm/dL), moderate (7-9.9 gm/dL) and severe degree (below 7 gm/dL) anaemia according to haemoglobin values. For thrombocytopenia, all females were divided into mild (100-150×109 /L), moderate (50-100×109 /L) and severe (below 50×109 /L) category according to platelet level. Results: Total 500 pregnant females were included in this study. Out of these, total 351 (70.2%) females were anaemic and 26 females (5.2%) were thrombocytopenic. Most of the anaemic pregnant females were from 26 to 32 years age group (47%) followed by 18 to 25 year age group (34%) and 33 to 40 year age group (19%). Conclusion: It is important to diagnose the effects of anaemia and thrombocytopenia in pregnant females and their offsprings. Anaemia in pregnancy is a challenging health problem in India, particularly in rural area. Early correction of anaemia and thrombocytopenia can reduce maternal morbidity and mortality","PeriodicalId":31115,"journal":{"name":"National Journal of Laboratory Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71270440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of CO-RADS Score with Inflammatory Markers in COVID-19 Patients: A Retrospective Analysis at a Designated COVID Centre of Kolhapur, India","authors":"Sapna S Deshpande, Namrata B Mestri, C. Patil","doi":"10.7860/njlm/2022/51293.2598","DOIUrl":"https://doi.org/10.7860/njlm/2022/51293.2598","url":null,"abstract":"Introduction: Coronavirus Disease-2019 (COVID-19) Reporting and Data System (CO-RADS) score in Computed Tomography (CT) scan of the chest is a priority investigation in early identification of the disease. Although, a CT scan gives a clear parenchymal picture of the condition, it has its disadvantages of the cost and specificity in the prognosis of COVID-19. In addition, clinical studies have revealed its decreased utility in assessing the severity of the disease. Aim: To understand the correlation of the CO-RADS score with various inflammatory markers and explore the changes in CO- RADS score with the severity of COVID-19 infection. Materials and Methods: The present study is a retrospective observational study in a designated COVID-19 centre in Kolhapur, India. The radiological and pathological records of 64 cases for two months (September to October 2020) were reviewed and charted. The present study included all the cases over 18 years. The authors excluded pregnant women, patients with tuberculosis, interstitial lung disease, and pulmonary malignancy with a view of interference with the radiological presentation of COVID- 19. Along with demographics, biomarkers like Total Leucocyte Count (TLC), C-Reactive Protein (CRP), Lactate Dehydrogenase (LDH), D-Dimer, Interleukin-6 (IL-6), Procalcitonin (PCT), and serum ferritin were retrospectively reviewed and documented. The CO-RADS score, as reported by an expert radiologist, was noted down. Pearson’s correlation coefficient was used to correlate the CO-RADS score with various inflammatory markers. Results: The authors found significant high positive correlation of CT score with LDH (r=0.754; p-value <0.001), moderate positive correlation with IL-6 (r=0.503; p-value <0.001), low positive correlation with CRP (r=0.477; p-value <0.001) and PCT (r=0.461; p-value <0.001). The correlation between the CT score with serum ferritin (r=0.284; p-value=0.023), total leukocyte count (r=0.260; p-value=0.038) and D-dimer (r=0.242; p-value 0.050) was negligible. Conclusion: The CO-RADS CT score is associated with the severity of COVID-19 disease and with mortality. The CO-RADS score showed a high positive correlation with LDH values of the present study. The LDH seems to be a promising marker and has to be further evaluated in assessing early COVID-19 infection with a multi-centric and more extensive sample size approach.","PeriodicalId":31115,"journal":{"name":"National Journal of Laboratory Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71270491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Calcium and Phosphate Ionic Product as an Early Marker of Vascular Calcification to Predict Cardiac Risk in Chronic Kidney Disease: A Case-control Study","authors":"M. Deepthi, M. Sirsikar, A. Shailaja, Shrabani Mohanthy","doi":"10.7860/njlm/2022/52862.2622","DOIUrl":"https://doi.org/10.7860/njlm/2022/52862.2622","url":null,"abstract":"Introduction: Cardiovascular disease is the most leading cause of death in patients with Chronic Kidney Disease (CKD) and Vascular Calcification (CV) is one of the strongest predictors of cardiovascular risk. CV is a process characterised by thickening and loss of elasticity of muscular arteries walls which occurs in two distinct sites, the intimal associated with atherosclerotic plaques and medial calcification is characterised by vascular stiffening and arteriosclerosis with adverse clinical outcomes leading to cardiovascular mortality. Disturbed mineral metabolism such as increased serum phosphorus and ionic product may be one such risk factor and is emerging as a principle modifier of CV in the CKD subjects. Aim: To determine serum phosphorus and calcium in CKD patients and compare with calcium phosphorus ionic product as an early independent marker of CV in CKD to predict cardiac mortality. Materials and Methods: This was duration based case-control study conducted in Department of Nephrology, Vydehi Institute of Medical Sciences & Research Centre, Karnataka, India. Fifty cases of CKD presented in stage 3,4 and 5 and 50 healthy individuals between the age group 21-78 years were included as controls. Serum levels of Calcium, Phosphorous were analysed in autoanalyser, Ca x P ionic product was calculated, estimated Glomerular Filtration Rate (eGFR) was calculated by Modification of Diet in Renal Disease (MDRD) formula. All measured variables were compared with eGFR and compared between cases and controls. The results were presented as a mean±Standard Deviation (SD) and p-value <0.05 was considered as significant. Results: Mean age of cases were 47.74±11.01 years and 45.66±11.46 years were of controls. Clinically, confirmed CKD was found more in male patients 31 (62) compared to female 19 (38). eGFR (mL/min) cases (14.12±10.72) and (102.97±27.46) in control, Serum Creatinine (mg/dl) were 7.04±5.34 mg/dL in cases which was significantly more compared to control (0.84±0.20 mg/ dL). Serum Calcium (Ca) (mg/dL) 8.11±1.09 mg/dL in cases less compared with control 9.31±0.42 mg/dL, indicating hypocalcaemia. Serum Phosphrous (P) 5.2932±1.83 mg/dL) in CKD suggesting hyperphosphatemia compared to control (3.27±0.54 mg/dL). Calcium Phosphorus Ionic Product was 46.9108±14.77 in cases, elevated in CKD as compared to control (30.46±5.03). Statistically, significant result was found between serum phosphorus and calcium phosphorus ionic product (p<0.05), well compared with eGFR of stage 3, 4 and 5 CKD patients. Conclusion: Hyperphosphatemia and elevated calcium- phosphate ionic product is an early independent marker of calcification to predict cardiovascular risk in late stages of CKD.","PeriodicalId":31115,"journal":{"name":"National Journal of Laboratory Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71270611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histomorphological Study of Ovarian Lesions with Emphasis on Rare Entities- A Descriptive Study","authors":"S. Surapaneni, Vandana Gangadharan, Harika Pentakota, Krishna Kumari Sala","doi":"10.7860/njlm/2022/53606.2657","DOIUrl":"https://doi.org/10.7860/njlm/2022/53606.2657","url":null,"abstract":"Introduction: The ovaries are bilateral organs on either side of the uterus. Non neoplastic and neoplastic lesions occur in all age groups and can present with similar clinical and radiological features. Histopathology remains the gold standard for diagnosing and categorising these lesions. Aim: To study the prevalence of ovarian lesions in relation to age and clinical findings. Neoplastic lesions will be histologically classified as per World Health Organisation guidelines. Findings in this study will be compared with that in other studies. Materials and Methods: This is an observational study of all ovarian lesions undertaken in the Department of pathology in NRI Institute of Medical Sciences, Sangivalasa, Vishakhapatnam, Andhra Pradesh, India, from June 2018 to November 2021. Specimens were received as ovarian masses alone, or in combination with hysterectomy with either unilateral or bilateral salpingo- oophorectomy. Relevant clinical information was obtained from the records. The tissues were processed by using an automatic tissue processor, paraffin blocks were made and sections cut were stained with Haematoxylin and Eosin(H&E) and examined under the microscope. The lesions were categorised as non neoplastic and neoplastic. The neoplastic lesions were classified according to the latest 2020 World Health Organisation (WHO) guidelines. Incidence of various lesions was expressed in percentage. Results: A total of 119 specimens were studied. Some of the specimens had bilateral ovaries and the second ovary had a different nonneoplastic lesion in 18 cases. 80 (60.60%) were nonneoplastic lesions and 52 (39.39%) were neoplastic lesions. 5 cases of torsion could not be classified into neoplastic or non-neoplastic due to lack of viable histological features. Non neoplastic lesions were more common in the 31-50 years age group. Benign neoplasms were common in 41-50 years age group. Malignant tumours were more common in the perimenopausal and postmenopausal age group. The most common non neoplastic lesion were 39 cases (48.75%) of follicular cyst followed by 26 cases (32.5%) of corpus luteal cyst, 13 cases (16.25%) of cystic follicles and 2 cases (2.5%) of endometriotic lesions. Of the neoplastic lesions, 47 cases (90.38%) were benign, 2 cases (3.84%) were borderline and 3 cases (5.76%) were malignant tumours. Surface epithelial tumours were 42 cases (80.76%), followed by 6 cases (11.53%) of germ cell tumours and 4 cases (7.69%) of sex cord stromal tumours. Conclusion: Non neoplastic lesions and neoplastic lesions present with similar clinical and radiologic picture and must be differentiated histopathologically. Non neoplastic lesions were more common in the present study. Among the neoplasms, benign tumours were more common and surface epithelial tumours were more common than any other category. Torsion ovary, Seromucinous cystadenoma, atypical endometrioid tumor and granulosa cell tumor were some rare entities that were encountered in the study. Cla","PeriodicalId":31115,"journal":{"name":"National Journal of Laboratory Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71270696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathological Spectrum of Endoscopic Gastrointestinal Biopsies: An Experience from a Tertiary Care Centre, Uttarakhand, India","authors":"N. Bahal, A. Malviya, S. Ahuja","doi":"10.7860/njlm/2022/52480.2635","DOIUrl":"https://doi.org/10.7860/njlm/2022/52480.2635","url":null,"abstract":"Introduction: The Gastrointestinal Tract (GIT) is the site of numerous pathological lesions from inflammatory to malignant. Endoscopic biopsy plays an important role in detection of early- stage cancers and precursor lesions. It also aids in determining the extent of disease, monitoring response to therapy and for early detection of complications. However, histopathological evaluation serves as gold standard for diagnosis and aids the clinician in deciding further management. Aim: To determine the histopathological spectrum of gastrointestinal lesions and assess the frequency of benign and malignant tumours in GIT in a tertiary care center in North India. Materials and Methods: It was a retrospective study in which all endoscopic gastrointestinal biopsies received in Department of Pathology at Shri Guru Ram Rai Institute of Medical and Health Sciences from January 2015 to December 2017 were retrieved. The lesions from oropharynx, oesophagus, stomach, small intestine, large intestine, rectum and anal canal were categorised as neoplastic (further divided as benign and malignant) and non neoplastic. The results were tabulated and expressed as frequencies and percentages. Results: Of the 867 cases, 582 were males and 285 were females with a male to female ratio of 2.1:1. The most common age group was sixth decade with a mean age of 53.8 years. Out of a total of 7782 specimens received, 867 (11.1%) were endoscopic gastrointestinal biopsies. Among all the biopsies evaluated, 116 (13.4%) were from oropharynx, 55 (6.3%) oesophageal, 97 (11.2%) from stomach and Gastro-Oesophageal (GE) junction, 138 (15.9%) small intestinal and 461 (53.2%) from colorectum and anal canal. Out of 867 biopsies, 670 (77.3%) were classified as non neoplastic and 197 (22.7%) were neoplastic. Incidence of malignancy was highest in gastric (29%) and oropharyngeal (28.4%) biopsies while it was least in small intestinal biopsies (4.3%). Conclusion: In the current study, squamous cell carcinoma and ulcerative colitis were the most prevalent neoplastic and non neoplastic lesions, respectively. It is advisable to interpret and correlate endoscopic findings with histopathology to arrive at a final diagnosis and aid the clinician for further management.","PeriodicalId":31115,"journal":{"name":"National Journal of Laboratory Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71270925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Vancomycin MICs by Broth Microdilution Method, E-Test and Vitek 2C among MRSA Isolates in Tertiary Care Centre, Hyderabad, India","authors":".. Bushra, K. Padmaja, S. Sudhaharan, V. Teja","doi":"10.7860/njlm/2022/53225.2627","DOIUrl":"https://doi.org/10.7860/njlm/2022/53225.2627","url":null,"abstract":"Introduction: The most important pathogen isolated from Skin and Soft Tissue Infections (SSTIs) is a Gram positive organism, Staphylococcus aureus (S.aureus). Wide range of emerging Methicillin Resistant Staphylococcus aureus (MRSA) infections is leading to global threat causing Community Acquired-MRSA (CA-MRSA) or Hospital Acquired MRSA (HA-MRSA). Minimum Inhibitory Concentration (MIC) is done to ensure that antibiotics are chosen efficiently in the clinical settings by Vitek 2, Epsilometer- test (E-test) and Broth Microdilution (BMD) method. Aim: To determine the clinical spectrum of MRSA and comparison of Vancomycin MICs obtained by E-test, Vitek 2C and BMD method. Materials and Methods: This was a cross-sectional study that was conducted at Nizam’s Institute of Medical Sciences, Hyderabad, Telangana, India during the period of November 1st 2019 to December 31st, 2020 from Inpatient Department (IPD) and Outpatient Department (OPD). Among 464 SSTIs, 132 were S.aureus, out of which 38 isolates were MRSA. Identification and sensitivity of the isolates such as pus, wound swabs, and tissues were identified through Vitek 2 compact system. Statistical analysis of the demographic and clinical characteristics was represented as frequency and percentages. Results: A total of 464 SSTIs, S.aureus were 132 of which MRSA were 38 (29%) and Methicillin sensitive Staphylococcus aureus (MSSA) were 94 (71%) with male predominance of 29 (76.3%) with MRSA. Most of the patients were in the age group of 21- 30 years (26%). Amongst the total 38 patients analysed,25 were from IPD and 13 were from OPD. A 23/38 wound swabs (60%) 12/38 of pus (28%) and 3/38 (7%) were tissues. The predominant risk factor observed was Surgical Site Infections (SSI) in 19 cases (50%) followed by prior antibiotic therapy in 17 (44%) cases. The median duration of hospitalisation was 31.5 days. Vancomycin susceptibility by all three methods with an MIC range of 0.5-2μg/ ml by all three methods, except for one isolate where the MIC was >32ug/ml by Vitek 2C and 8 ug/mL by E-test, which was sensitive by BMD with an MIC of 0.25 μg/mL. Conclusion: Implementing infection control practices and controlling the risk factors will help in management of MRSA infections. Drug resistance to Glycopeptides can be avoided by regular screening of vancomycin creeps by different susceptibility methods in order to avoid treatment failures.","PeriodicalId":31115,"journal":{"name":"National Journal of Laboratory Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71271003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Importance of Serosal Fluid Cytology as an Aid to Primary Diagnosis: A Descriptive Cross-sectional Study","authors":"Sheetal Maheshkumar Sale, V. Mane, Poornima Kadam","doi":"10.7860/njlm/2022/55027.2613","DOIUrl":"https://doi.org/10.7860/njlm/2022/55027.2613","url":null,"abstract":"Introduction: Cytological study of fluids is an inexpensive, simple procedure and has significant utility in diagnosing neoplastic and non neoplastic lesions. The cytological examination of fluids in combination with physical examination helps identify aetiologic agents, follow the natural process of the disease and monitor the response to the treatment. Aim: To determine the diagnostic utility of serosal fluid cytology and analyse the incidence of neoplastic and non neoplastic lesions using serous fluid cytology. Materials and Methods: This was a descriptive cross-sectional study comprising 375 cases conducted in a tertiary care hospital, Sangli, Maharashtra, India, from August to July 2021. Purposive sampling was used to recruit the participants. All the patients with pleural effusions, ascites or in whom Cerebral Spinal Fluid (CSF), pericardial and synovial fluids examination was indicated were included. The provisional diagnosis was obtained from case sheets, including relevant clinical information. Smears were prepared from freshly tapped specimens without adding anticoagulants and were processed by routine, conventional smear technique. The data were analysed using the SPSS version 22.0 for Windows. Numerical variables were reported as frequency and percentage. The chi-square test was used wherever necessary, and the p-value less than 0.05 were considered significant. Results: The peritoneal fluid was the most common fluid collected in the present study, followed by pleural fluid and CSF. The malignancy rate in the present study was 19 (10.4%) of peritoneal fluid, 6 (5.9%) for pleural fluid, and 2 (4.1%) for CSF. Adenocarcinoma was the most common malignancy found in present study. Conclusion: Adenocarcinoma was the most common malignancy found in this study, which was in concordance with the research conducted earlier, where gold standard investigations confirmed the findings. In the peritoneal fluid, most of the patients had cirrhosis and tuberculosis. In pleural fluid and cerebrospinal fluid, most of them had tuberculosis and chronic inflammatory conditions, respectively. Previous researchers confirmed similar findings in their studies. It is seen that malignant and benign conditions like tuberculosis can be diagnosed well with effusion cytology.","PeriodicalId":31115,"journal":{"name":"National Journal of Laboratory Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71271153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histomorphological Spectrum of Endometrial Tissue in Abnormal Uterine Bleeding","authors":"Jyoti Brahmaiah, Lalitha Sree Kuppareddy, Vijay Kumar Papireddygari, C. Begum","doi":"10.7860/njlm/2022/57254.2674","DOIUrl":"https://doi.org/10.7860/njlm/2022/57254.2674","url":null,"abstract":"Introduction: Abnormal Uterine Bleeding (AUB) is a very common presenting complaint in patients visiting the gynaecologists all over the world. Though common, AUB can cause tremendous physical as well as emotional distress to the patient. Aim: To study the histomorphological pattern of endometrium in patients with AUB. Materials and Methods: This study was a retrospective study conducted during a six month period from October 2021 to March 2022 in the Department of Pathology at Apollo Institute of Medical Sciences and Research, Chittoor, Andhra Pradesh, India, on 210 endometrial tissue samples of women who presented with AUB. Specimens that were received during a one year period between March 2019 and February 2020 were processed routinely and stained using Haematoxylin and Eosin stain. Detailed microscopic evaluation was done and eleven different histopathological diagnoses were made. The data collected was entered into Microsoft Excel sheet and percentages were analysed manually. Results: Maximum number of cases of AUB were seen in the 41-50 years age group. The most common histopathological pattern in this study was proliferative phase seen in 67 cases (31.90%) followed by endometrial hyperplasia which was seen in 42 cases (20.00%). The other important patterns included pregnancy related complications, 32 (15.24%), secretory phase, 24 (11.43%), disordered proliferative endometrium, 14 (6.67%), chronic endometritis 11 (5.24%) and hormonal/pill endometrium, 8 (3.81%). Endometrial malignancy was diagnosed only in one case (0.48%). Conclusion: A wide variety of histopathological patterns were found in endometrial samples in AUB across different age groups. Histopathological examination of endometrium is an important tool in the diagnosis and management of AUB.","PeriodicalId":31115,"journal":{"name":"National Journal of Laboratory Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71271262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coagulase Negative Staphylococcus Species Isolated from Blood Culture: A Pathogen or a Contaminant?","authors":"Pranjali Sanjay Bhosle, V. Thakar, M. Modak","doi":"10.7860/njlm/2022/55619.2671","DOIUrl":"https://doi.org/10.7860/njlm/2022/55619.2671","url":null,"abstract":"Introduction: Coagulase negative staphylococci (CoNS) are the most frequent blood culture isolates and an important cause of nosocomial blood stream infections especially in catheterised patients. CoNS are also the most common contaminants of blood cultures and are proven to be especially problematic. These uncertainties may result in over diagnosis and indirect overuse of antimicrobials especially vancomycin. Aim: To determine the clinical significance of CoNS isolated from blood culture of patients admitted in a tertiary care hospital. Materials and Methods: A prospective cross-sectional study was conducted in Bharati Hospital Research Center, Pune, Maharashtra, India, from August 2019 to July 2020 (One year). Patients whose paired/multiple blood culture samples showed pure growth of CoNS were included in the study. CoNS were identified by Vitek 2 system and antimicrobial susceptibility was reported. Clinical history of all patients was taken who showed pure growth of CoNS in either one or multiple sites of the automated blood culture bottles. Isolated CoNS were considered as pathogen only if clinical and laboratory parameters are fulfilled. Chi-square test was used to find out statistical significance of isolated pathogenic CoNS. Results: A total 147 CoNS isolated from blood cultures of suspected patients of sepsis were included in study. About 23 (15.6%) CoNS were isolated from both the sites. Remaining 124 (84.4%) CoNS were isolated from single site. CoNS were considered as pathogen in all 23 cases based on clinical and laboratory criteria. The most frequent isolated CoNS was S. haemolyticus 7 (30.4%), followed by S. epidermidis 5 (21.7%), S. hominis 3 (13.1%) , S. lugdunensis 3 (13.1%), S. scuiri 2 (8.7%), S. xylosus 1 (4.3%), S. caprae 1 (4.3%), S. capitis 1 (4.3%). Methicillin resistance was observed in 15 (65.2%) CoNS strains. Conclusion: Patients suffering from CoNS from their blood stream should be carefully evaluated clinically. Proper blood collection techniques need to be followed to avoid contamination of blood culture samples and to avoid diagnostic dilemma.","PeriodicalId":31115,"journal":{"name":"National Journal of Laboratory Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71271351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Clinico-pathological Study of Papulonodular Lesions of Skin in a Rural Hospital Setup","authors":".. Rubina, R. Parthibhan, R. Sujitha, Savita Dongapure","doi":"10.7860/njlm/2022/58125.2669","DOIUrl":"https://doi.org/10.7860/njlm/2022/58125.2669","url":null,"abstract":"Introduction: Skin disorders are a common cause of morbidity in our country, High prevelance of dermatological lesions is seen in tropical countries. The spectrum of lesions varies significantly depending on the geographical region, so accurate diagnosis is of utmost importance as treatment is varied for different skin disorders presenting with similar clinical findings. Aim: To study the spectrum of papulonodular lesions of skin and to evaluate concordance between the clinical and histopathological diagnosis of papulonodular skin lesions. Materials and Methods: This prospective cross-sectional study was conducted in Department of Pathology at MVJ Medical College and Research Hospital, Hoskote, Karnataka, India, from October 2017 to September 2019. The study included 100 skin biopsies that had clinically presented as papules, nodules and as papulonodular lesions. Based on the histopathological findings the lesions were grouped according to aetiology and the final histopathological diagnosis was compared with the clinical diagnosis offered. The slides were stained with Haematoxylin and Eosin, examined under a microscope and findings were noted. Special stains on the tissue sections like Zeihl-Neelson and Fite Faraco were used when required. The qualitative characteristics presented using frequency and percentages, quantitative variables presented using Mean±SD. Results: In the 100 biopsies studied 77 cases were papular, 20 cases were nodular and 3 cases were both papular and nodular. The lesions were common in males (54%) with 67% being in the 21-50 years age group. Lesions were categorised into five aetiological groups based on histology i.e, non infectious papulosquamous (25%), eczematous (23%, Inflammatory (20%), Infectious (11%) and lastly neoplastic (21%). A clinico- pathological association of 79% was observed with differences mainly observed in the eczematous group and tumours. Conclusion: This study highlights the various common skin disorders that can present as papulonodular lesions and the significance of histopathological examination and clinico- pathological association for early diagnosis and management of skin lesions.","PeriodicalId":31115,"journal":{"name":"National Journal of Laboratory Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71271365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}