Biomacromolecules最新文献_第7页

A Pectin-Responsive Aggregation-Induced Emission Probe for Specific Staining of Plant Cell Walls. 用于植物细胞壁特异性染色的果胶响应聚集诱导发射探针。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-05-07 DOI: 10.1021/acs.biomac.5c00021

Xinling Liu, Shuang Shen, Liang Shao, Yan Zhang, Xianpeng Zhang, Xingqiang Lü, Li Xu, Guanying Li

引用次数: 0

Programmable Fabrics of Enzyme-Responsive Amphiphiles: A Multiscale Platform for Hierarchical Mesophase Transformations. 酶反应性两亲植物的可编程结构：分层中间相转化的多尺度平台。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-05-07 DOI: 10.1021/acs.biomac.4c01649

Nicole Edelstein-Pardo, Shira Kutchinsky, Amit Sitt, Roey J Amir

{"title":"Programmable Fabrics of Enzyme-Responsive Amphiphiles: A Multiscale Platform for Hierarchical Mesophase Transformations.","authors":"Nicole Edelstein-Pardo, Shira Kutchinsky, Amit Sitt, Roey J Amir","doi":"10.1021/acs.biomac.4c01649","DOIUrl":"https://doi.org/10.1021/acs.biomac.4c01649","url":null,"abstract":"Systems capable of undergoing a controlled cascade of mesophase transitions across hierarchical scales represent a novel class of dynamic materials. Here, we describe an electrospun polymeric fabric composed of enzyme-responsive di- and triblock copolymers that undergoes a hierarchical cascade of four distinct mesophases. Initially, on immersion in water, the macroscale fabric dissolves, forming nanoscale micelles. Enzymatic degradation of the diblock components triggers a transition into a triblock-based hydrogel. Finally, the enzymatic degradation of the hydrogel into hydrophilic polymers leads to complete dissolution. By adjusting the di- and triblock ratios, we can finely tune the fabric's dissolution rate. Moreover, the fibers can encapsulate hydrophobic agents, which are retained within the micelle and hydrogel phases, enabling their controlled release. This cascade of mesophase transitions, from a macroscopic solid to nanoscale assemblies, organized hydrogels, and eventual molecular dissolution, demonstrates sophisticated hierarchical control, unlocking new opportunities for biomedical applications of programmable materials.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural Basis of SARS-CoV-2 Nsp13-Derived Peptide-Mediated NK Cell Activation. SARS-CoV-2 nsp13衍生肽介导NK细胞活化的结构基础

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-05-07 DOI: 10.1021/acs.biomac.5c00168

Xiaole Xu, Song Luo, Jinxin Liu, Enhao Zhang, Houde Liang, Lili Duan

{"title":"Structural Basis of SARS-CoV-2 Nsp13-Derived Peptide-Mediated NK Cell Activation.","authors":"Xiaole Xu, Song Luo, Jinxin Liu, Enhao Zhang, Houde Liang, Lili Duan","doi":"10.1021/acs.biomac.5c00168","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00168","url":null,"abstract":"As pivotal effectors of antiviral immunity, natural killer (NK) cells are crucial for controlling the spread of COVID-19. The nonstructural protein 13 of SARS-CoV-2 can encode a viral peptide (Nsp13232-240) preventing human leukocyte antigen E (HLA-E) from recognizing inhibitory receptor NKG2A, thereby activating NK cells. The underlying molecular mechanisms of Nsp13232-240 remain unclear. Therefore, we compared the interaction discrepancy between the self-peptide and Nsp13232-240, theoretically predicting its source. Results indicate that electrostatic interaction energy provides the main source of binding, and its attenuation greatly promotes binding affinity differences. Nsp13232-240 disrupts the hydrogen bond network between CD94 and HLA-E, impacting the binding of hot-spot residues, including Q112CD94 and E161HLA-E. Moreover, Nsp13232-240 breaks the salt bridges formed by K217NKG2A and K199NKG2A with HLA-E. Conformational changes induced by Nsp13232-240 lead to diminished atomic contacts and an unstable binding pattern. These findings provide novel insights into the immunomodulatory role of Nsp13232-240 and may inform future NK cell-mediated strategies targeting SARS-CoV-2.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly Efficient Thiol-Michael Addition Post-Modification toward Potent Degradable Antibacterial Polyesters with Guanidine Moiety. 高效巯基加成后改性制备胍基可降解抗菌聚酯。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-05-07 DOI: 10.1021/acs.biomac.5c00461

Yilin Qian, Wei Li, Yang Cheng, Xiao-Tuan Zhang, Fu-Sheng Du, Zi-Chen Li

{"title":"Highly Efficient Thiol-Michael Addition Post-Modification toward Potent Degradable Antibacterial Polyesters with Guanidine Moiety.","authors":"Yilin Qian, Wei Li, Yang Cheng, Xiao-Tuan Zhang, Fu-Sheng Du, Zi-Chen Li","doi":"10.1021/acs.biomac.5c00461","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00461","url":null,"abstract":"We have previously synthesized poly(3-methylene-1,5-dioxepan-2-one) (PMDXO) that could be modified through the thiol-Michael addition reaction to afford versatile degradable polymers. Herein, we find that the γ-oxa in PMDXO exerts a dramatically accelerating effect on the thiol-Michael addition post-modification, which makes PMDXO a promising platform polymer for synthesizing guanidinium-functionalized aliphatic polyesters under mild and approximately stoichiometric conditions. The relationship between polymer structure and antibacterial performance was investigated. A promising cationic polyester, P20-2C, which shows extremely low hemolytic activity, moderate cytotoxicity, and broad-spectrum potent bactericidal capability against 214 clinically isolated ESKAPE strains, is obtained. The good biocompatibility and potent in vivo antibacterial efficacy of P20-2C have been demonstrated in mice using three bacterial infection models, including MDR E. coli-infected peritonitis and MRSA-infected subcutaneous abscess and skin wound. Finally, a multimodal bactericidal mechanism of membrane disruption plus reactive oxygen species upregulation is proposed for P20-2C against E. coli and S. aureus.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strong Association between Proanthocyanidins and Polysaccharides in the Cell Walls of Western Redcedar Bark. 西部红杉树皮细胞壁中原花青素与多糖的密切联系。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-05-06 DOI: 10.1021/acs.biomac.5c00271

Gio Ferson M Bautista, Oliver Musl, Michael L A E Easson, Lars H Kruse, Harley Gordon, Markus Bacher, Ivan Sumerskii, Aude A Watrelot, Jörg Bohlmann, Antje Potthast, Thomas Rosenau, Orlando J Rojas

{"title":"Strong Association between Proanthocyanidins and Polysaccharides in the Cell Walls of Western Redcedar Bark.","authors":"Gio Ferson M Bautista, Oliver Musl, Michael L A E Easson, Lars H Kruse, Harley Gordon, Markus Bacher, Ivan Sumerskii, Aude A Watrelot, Jörg Bohlmann, Antje Potthast, Thomas Rosenau, Orlando J Rojas","doi":"10.1021/acs.biomac.5c00271","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00271","url":null,"abstract":"The co-occurrence of polysaccharides and proanthocyanidins in the aqueous extracts of western redcedar (Thuja plicata Donn; WRC) bark limits their commercial utilization. To better understand their association, proanthocyanidins and polysaccharides were extracted with cold water (3.4% w/w bark) and isolated as an alcohol-insoluble residue (AIR, 1.0% w/w bark). The polysaccharide content (∼30% w/w AIR) was analyzed by acidic and enzymatic depolymerization, revealing the presence of pectins, xyloglucans, and xylans. NMR spectroscopy identified features, such as acetylation and methyl esterification. Thiolysis followed by HPLC-DAD revealed that proanthocyanidins (1.46% w/w AIR) exhibit a mean degree of polymerization of 5.3, a cis/trans ratio of 0.40, and a procyanidin/prodelphinidin ratio of 3.90. This study provides a detailed structural characterization of proanthocyanidins and polysaccharides in the AIR of WRC bark. The findings highlight their strong association, which may contribute to distinctive properties that warrant further exploration, particularly in efforts to valorize bark residues.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of Folate-Modified Carboxymethyl Chitosan-Sinomenine-Curcumin Nanopolymer for Targeted Treatment of Rheumatoid Arthritis. 叶酸修饰羧甲基壳聚糖-青藤素-姜黄素纳米聚合物靶向治疗类风湿性关节炎的研究。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-05-05 DOI: 10.1021/acs.biomac.4c01701

Jiamei Tang, Sihui Li, Yulu Wang, Minghao Yuan, Yan Wan, Xue Liang, Li Guo, Yiping Guo

{"title":"Study of Folate-Modified Carboxymethyl Chitosan-Sinomenine-Curcumin Nanopolymer for Targeted Treatment of Rheumatoid Arthritis.","authors":"Jiamei Tang, Sihui Li, Yulu Wang, Minghao Yuan, Yan Wan, Xue Liang, Li Guo, Yiping Guo","doi":"10.1021/acs.biomac.4c01701","DOIUrl":"https://doi.org/10.1021/acs.biomac.4c01701","url":null,"abstract":"Sinomenine hydrochloride (SH) has been clinically utilized for many years to treat rheumatoid arthritis (RA) in both oral and injectable forms. However, its low bioavailability, poor targeting, high dosage requirements, and side effects, present significant challenges. This study developed folic acid-carboxymethyl chitosan-modified sinomenine-curcumin nanopolymers (named SCNP) for the targeted treatment of RA, to reduce dosage and side effects. The design of SCNP employs folic acid (FA) as a targeting moiety, facilitating specific binding to the folate receptor (FR) on the surface of macrophages and enabling internalization into activated macrophages via endocytosis, thereby achieving targeted delivery to sites of inflammation. In a rat and cell model of RA, SCNP was found to decrease reactive oxygen species (ROS) and pro-inflammatory factors while increasing the anti-inflammatory factor IL-10 through the NF-κB/NLRP3 pathway. These findings indicate that SCNP has the potential to lower drug dosage, enhance therapeutic efficacy, and minimize side effects such as diarrhea and rash, thereby highlighting its promise as an inflammation-targeting nanopolymer.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light-Triggered Cross-Linking of Linear Poly(ethylene glycol) Hydrogels for Enhanced Antibacterial Delivery. 光触发交联线性聚乙二醇水凝胶增强抗菌递送。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-05-02 DOI: 10.1021/acs.biomac.5c00266

Siyuan Liu, Yuan Tian, Kaijie Zhao, Peiyu Zhang, Jia Li, Jiwei Cui

引用次数: 0

Lead Informed Artificial Intelligence Mining of Antitubercular Host Defense Peptides 抗结核宿主防御肽的人工智能先导挖掘

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-05-01 DOI: 10.1021/acs.biomac.5c0024410.1021/acs.biomac.5c00244

Diptomit Biswas, Sara Benson, Aidan Matunis, Gebremichal Gebretsadik, Adam Wertz, Ben J. StPierre, Nathan Schacht, Yue Yan, Hanna Y. Gebremichael, Pak Kin Wong, Anthony D. Baughn and Scott H. Medina*,

{"title":"Lead Informed Artificial Intelligence Mining of Antitubercular Host Defense Peptides","authors":"Diptomit Biswas, Sara Benson, Aidan Matunis, Gebremichal Gebretsadik, Adam Wertz, Ben J. StPierre, Nathan Schacht, Yue Yan, Hanna Y. Gebremichael, Pak Kin Wong, Anthony D. Baughn and Scott H. Medina*, ","doi":"10.1021/acs.biomac.5c0024410.1021/acs.biomac.5c00244","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00244https://doi.org/10.1021/acs.biomac.5c00244","url":null,"abstract":"Identifying host defense peptides (HDPs) that are effective against drug-resistant infections is challenging due to their vast sequence space. Artificial intelligence (AI)-guided design can accelerate HDP discovery, but it traditionally requires large data sets to operationalize. We report an AI workflow that utilizes limited data sets (∼100 peptides) to uncover potent, selective, and safe HDPs by informing selection through lead candidate mutational scanning. This approach, referred to as Lead Informed Machine Interrogation of Therapeutic Sequences (LIMITS), is applied against the exemplary pathogen Mycobacterium tuberculosis. Experimental validation of predicted sequences shows nearly an order of magnitude improvement in potency, selectivity, and safety, relative to the initial template. Post hoc analysis suggests sequence length may be a unique and underappreciated driver of antitubercular HDP activity. These results demonstrate that, with continued development, the LIMITS approach can identify selective HDPs under data-limited conditions and elucidate structure–function–performance relationships previously hidden in biologic complexity.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":"26 5","pages":"3167–3179 3167–3179"},"PeriodicalIF":5.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acs.biomac.5c00244","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143934208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Facile and Versatile Platform for Cytosolic Delivery of Proteins in Nanoshells of DNA or RNA: Packaging Options in Multiplexed Delivery 在DNA或RNA的纳米壳中递送蛋白质的一个简单和通用的细胞质递送平台：多路递送的包装选择

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-05-01 DOI: 10.1021/acs.biomac.5c0014910.1021/acs.biomac.5c00149

Pilar O’Neal, Kareem Washington, Bram Estes and Preethi L. Chandran*,

{"title":"A Facile and Versatile Platform for Cytosolic Delivery of Proteins in Nanoshells of DNA or RNA: Packaging Options in Multiplexed Delivery","authors":"Pilar O’Neal, Kareem Washington, Bram Estes and Preethi L. Chandran*, ","doi":"10.1021/acs.biomac.5c0014910.1021/acs.biomac.5c00149","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00149https://doi.org/10.1021/acs.biomac.5c00149","url":null,"abstract":"Polyethylenimine (PEI) polymers are used to compact DNA into nanoparticles for delivery into cells. We have shown that PEI-mannose polymers compact DNA into nanoshell-like particles, which can load proteins as well. Here we show that these DNA containers are uniquely versatile for scavenging proteins, irrespective of size, charge, and hydrophobicity from dilute solutions. The number of DNA containers for loading proteins can be controlled independently of the protein loading per container by changing the amounts of DNA and protein in solution. This provides control of the fraction of cells receiving the payload and the relative amounts of DNA and protein per cell. The proteins released inside cells retain enzymatic activity. The proposed technology provides a new way to approach protein delivery by hitchhiking proteins within a facile and well-established DNA-delivery mechanism and by utilizing sugar biophysics to load a wide range of proteins in a single-step process.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":"26 5","pages":"3084–3103 3084–3103"},"PeriodicalIF":5.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143934554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reverse Block Sequence in Self-Immolative Poly(benzyl ether)-Based Amphiphiles for Tailoring End Groups and Self-Assembly Behavior 自焚聚苯醚基两亲体中端基裁剪和自组装行为的反向嵌段序列

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-04-30 DOI: 10.1021/acs.biomac.4c0184510.1021/acs.biomac.4c01845

Ji Woo Kim, Tae-Il Kang, Eunpyo Choi and Hyungwoo Kim*,

引用次数: 0