Biomacromolecules最新文献_第6页

Antibody-Recruiting Surfaces Using Adaptive Multicomponent Supramolecular Copolymers. 使用自适应多组分超分子共聚物的抗体招募表面。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-05-12 Epub Date: 2025-04-09 DOI: 10.1021/acs.biomac.5c00043

Marle E J Vleugels, Esmee de Korver, Simone I S Hendrikse, Sinan Kardas, Shikha Dhiman, Bas F M de Waal, Sandra M C Schoenmakers, Stefan Wijker, Bruno G De Geest, Mathieu Surin, Anja R A Palmans, E W Meijer

{"title":"Antibody-Recruiting Surfaces Using Adaptive Multicomponent Supramolecular Copolymers.","authors":"Marle E J Vleugels, Esmee de Korver, Simone I S Hendrikse, Sinan Kardas, Shikha Dhiman, Bas F M de Waal, Sandra M C Schoenmakers, Stefan Wijker, Bruno G De Geest, Mathieu Surin, Anja R A Palmans, E W Meijer","doi":"10.1021/acs.biomac.5c00043","DOIUrl":"10.1021/acs.biomac.5c00043","url":null,"abstract":"Multicomponent structures that mediate the clustering of antibodies on cancer cell surfaces are an attractive strategy to unleash innate immune killing mechanisms. However, covalent multifunctional scaffolds that combine cell surface anchoring and antibody binding can be challenging to synthesize and lack adaptability. Here, we present a dynamic multicomponent supramolecular system displaying both antibody- and cell surface-binding motifs, without covalent linkage between them. Supramolecular monomers based on benzene-1,3,5-tricarboxamide (BTA-(OH)3) were functionalized with benzoxaborole (Ba) for surface anchoring (BTA-Ba) or dinitrophenyl (DNP) for antibody binding (BTA-DNP1/3). The multicomponent fibers comprising BTA-(OH)3, BTA-Ba, and BTA-DNP1/3 recruited anti-DNP antibodies to sialic acid-functionalized supported lipid bilayers, indicating that both Ba and DNP remained accessible for binding. Dynamic exchange was demonstrated in a cell-mimicking environment, highlighting the adaptivity of these supramolecular polymers. Despite the complexity of a ternary system, the adaptivity of supramolecular polymers gives the individual components the possibility to act in concert, mimicking natural systems.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":"26 5","pages":"2971-2985"},"PeriodicalIF":5.5,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12076489/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Poly(2-(diethylamino)ethyl methacrylate)-Functionalized Carbon Nanodots as Theranostic Platforms for siRNA Delivery and Survivin Silencing in Triple-Negative Breast Cancer. 聚（2-（二乙胺）甲基丙烯酸乙酯）功能化碳纳米点作为三阴性乳腺癌siRNA递送和Survivin沉默的治疗平台

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-05-10 DOI: 10.1021/acs.biomac.5c00267

Paola Varvarà, Gennara Cavallaro, Nicolò Mauro

{"title":"Poly(2-(diethylamino)ethyl methacrylate)-Functionalized Carbon Nanodots as Theranostic Platforms for siRNA Delivery and Survivin Silencing in Triple-Negative Breast Cancer.","authors":"Paola Varvarà, Gennara Cavallaro, Nicolò Mauro","doi":"10.1021/acs.biomac.5c00267","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00267","url":null,"abstract":"This study describes the development of carbon nanodot (CDs)-based theranostic nanocarriers that integrate gene silencing with fluorescence imaging. Nitrogen- and sulfur-doped CDs were functionalized through controlled radical surface polymerization of 2-(diethylamino)ethyl methacrylate (DEAEMA), yielding self-tracking, cationic siRNA carriers CDs-pDEAEMA. The functionalization of CDs enhanced their fluorescence, broadening the emission spectrum toward the biologically transparent window. Fluorescent CDs-pDEAEMA effectively bound siRNA, remaining stable under physiological conditions, while in vitro studies proved their hemocompatibility and cytocompatibility on human dermal fibroblasts. Moreover, the ability to deliver BIRC5 siRNA was demonstrated in MDA-MB-231, successfully transfecting triple-negative breast cancer cells and resulting in an 80% reduction in the anti-apoptotic protein survivin. Furthermore, uptake studies demonstrated that the theranostic CDs are efficiently internalized in tumor cells and are clearly detectable by fluorescence imaging in the red region. These findings highlight the potential of CDs-pDEAEMA as an advanced theranostic tool for real-time tracking of siRNA therapy of breast cancer.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Replacing PEG-Lipid with Amphiphilic Polycarbonates in mRNA-Loaded Lipid Nanoparticles: Impact of Polycarbonate Structure on Physicochemical and Transfection Properties. 用两亲性聚碳酸酯取代含mrna的脂质纳米颗粒中的peg -脂质：聚碳酸酯结构对理化和转染性能的影响。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-05-10 DOI: 10.1021/acs.biomac.5c00088

Dao Thi Hong Le, Chuan Yang, Yue Zhang, Gui Zhao, Melgious J Y Ang, Ki Hyun Bae, James H P Hui, James L Hedrick, Yi Yan Yang

{"title":"Replacing PEG-Lipid with Amphiphilic Polycarbonates in mRNA-Loaded Lipid Nanoparticles: Impact of Polycarbonate Structure on Physicochemical and Transfection Properties.","authors":"Dao Thi Hong Le, Chuan Yang, Yue Zhang, Gui Zhao, Melgious J Y Ang, Ki Hyun Bae, James H P Hui, James L Hedrick, Yi Yan Yang","doi":"10.1021/acs.biomac.5c00088","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00088","url":null,"abstract":"Since the remarkable breakthrough of COVID-19 mRNA vaccines, lipid nanoparticles (LNPs) have gained substantial attention as the most cutting-edge clinical formulations for mRNA delivery. PEGylated lipid (PEG-lipid) has been regarded as an essential constituent of LNPs that helps to prolong their systemic circulation by preventing particle aggregation in the blood and sequestration by the mononuclear phagocyte system. Herein, we synthesized a series of mRNA-loaded nanoparticles by replacing ALC-0159 (a PEG-lipid used in the Comirnaty formulation) with amphiphilic PEG-polycarbonate diblock copolymers (PC-HNPs). Interestingly, variations of polycarbonate block length and structure significantly influenced mRNA encapsulation efficiency, transfection potency, colloidal stability, and PEG shedding rate of PC-HNPs. In vivo and ex vivo bioluminescence imaging revealed that upon subcutaneous administration in mice, the leading candidate PC3-HNP achieved lymph node accumulation comparable to that of the conventional ALC-0159-based LNP formulation while avoiding undesirable liver accumulation. Our findings may provide valuable information for the construction of next-generation nanocarriers for effective mRNA delivery.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-Dynamic Covalently Cross-Linked Polyglycerol Hydrogels for Tumor Spheroid Culture. 肿瘤球体培养用双动态共价交联聚甘油水凝胶。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-05-10 DOI: 10.1021/acs.biomac.4c01744

Jun Feng, Polina Ponomareva, Kunpeng Liu, Chuanxiong Nie, Rui Chen, Rainer Haag

引用次数: 0

Applying Computational Protein Design to Engineer Affibodies for Affinity-controlled Delivery of Vascular Endothelial Growth Factor and Platelet-Derived Growth Factor. 应用计算蛋白设计来工程修饰血管内皮生长因子和血小板衍生生长因子的亲和控制递送。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-05-09 DOI: 10.1021/acs.biomac.5c00097

Justin E Svendsen, Madeleine R Ford, Chandler L Asnes, Simon C Oh, Jonathan Dorogin, Karly M Fear, Johnathan R O'Hara-Smith, Lauren O Chisholm, Sophia R Phillips, Michael J Harms, Parisa Hosseinzadeh, Marian H Hettiaratchi

{"title":"Applying Computational Protein Design to Engineer Affibodies for Affinity-controlled Delivery of Vascular Endothelial Growth Factor and Platelet-Derived Growth Factor.","authors":"Justin E Svendsen, Madeleine R Ford, Chandler L Asnes, Simon C Oh, Jonathan Dorogin, Karly M Fear, Johnathan R O'Hara-Smith, Lauren O Chisholm, Sophia R Phillips, Michael J Harms, Parisa Hosseinzadeh, Marian H Hettiaratchi","doi":"10.1021/acs.biomac.5c00097","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00097","url":null,"abstract":"Vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF) play coordinated roles in angiogenesis. However, current biomaterial delivery vehicles for these proteins have a limited ability to precisely control the kinetics of protein release, preventing systematic exploration of their temporal effects. Here, we combined yeast surface display and computational protein design to engineer eight VEGF-specific and PDGF-specific protein binders called affibodies with a broad range of affinities for controlled protein release. Soluble affibodies modulated protein bioactivity as evidenced by changes in VEGF-induced endothelial cell proliferation and luminescent output of a PDGF-responsive cell line. Affibody-conjugated hydrogels enabled tunable protein release over 7 days. VEGF and PDGF released from affibody-conjugated hydrogels exhibited higher bioactivity than proteins released from hydrogels without affibodies, suggesting that these engineered affinity interactions could prolong protein bioactivity. This work underscores the power of computational protein design to enhance biomaterial functionality, creating a platform for tunable protein delivery.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis, Thermal and Mechanical Properties of Nonisocyanate Thermoplastic Polyhydroxyurethane Nanocomposites with Cellulose Nanocrystals and Chitin Nanocrystals. 纤维素纳米晶和几丁质纳米晶非异氰酸酯热塑性聚氨酯纳米复合材料的合成、热性能和力学性能

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-05-09 DOI: 10.1021/acs.biomac.5c00113

Pavithra M Wijeratne, Connie Ocando, Bruno Grignard, Lars A Berglund, Jean-Marie Raquez, Qi Zhou

{"title":"Synthesis, Thermal and Mechanical Properties of Nonisocyanate Thermoplastic Polyhydroxyurethane Nanocomposites with Cellulose Nanocrystals and Chitin Nanocrystals.","authors":"Pavithra M Wijeratne, Connie Ocando, Bruno Grignard, Lars A Berglund, Jean-Marie Raquez, Qi Zhou","doi":"10.1021/acs.biomac.5c00113","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00113","url":null,"abstract":"Incorporating biobased nanofillers including cellulose nanocrystals (CNCs) and chitin nanocrystals (ChNCs) into nonisocyanate polyurethane (NIPU) offers a multifunctional approach to improving mechanical and thermal properties while promoting sustainability and green chemistry. Nanocomposites of segmented thermoplastic polyhydroxyurethane (PHU) from vanillyl alcohol bis(cyclocarbonate) (VABC), poly(tetramethylene oxide) diamine (PTMODA), and bis(aminomethyl) norbornane (NORB) reinforced with a low amount of CNCs and partially deacetylated ChNCs were prepared and characterized. Fourier transform infrared spectroscopy, atomic force microscopy, and small-angle X-ray scattering revealed that partially deacetylated ChNCs were covalently grafted to the PHU through aminolysis of carbonate end groups in the hard segment, while CNCs were mixed with the PHU without interfacial covalent bonding. Consequently, the PHU/ChNC nanocomposites showed nanophase separation with smaller hard domains compared to neat PHU, while the PHU/CNC nanocomposites exhibited a phase-mixed system with broader interface regions. Dynamic mechanical analysis and tensile tests further revealed that the PHU/ChNC nanocomposites demonstrated a 49-fold increase in Young's modulus, a 20-fold increase in ultimate tensile strength, and a three-order-of-magnitude enhancement in storage modulus in the rubbery state compared to the PHU/CNC nanocomposites, highlighting the profound influence of interfacial covalent linkages in enhancing the thermal mechanical performance of segmented PHU.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis and Protective Mechanisms of Fluorescent Copolymers against Multi-Band UV-Induced Photodamage in HCE-T Cells. 荧光共聚物的合成及其对多波段紫外线诱导HCE-T细胞光损伤的保护机制

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-05-09 DOI: 10.1021/acs.biomac.4c01789

Wenqian Chen, Zi Ye, Lixiong Gao, Yuhang Jiang, Yue Wei, Xuejun Chen, Liqin Li, Tengfei Mao, Zhaohui Li

引用次数: 0

Highlighting the Influence between Physical and Chemical Cross-Linking in Dynamic Hydrogels for Two-Photon Micropatterning. 强调动态水凝胶中物理和化学交联对双光子微图的影响。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-05-08 DOI: 10.1021/acs.biomac.5c00062

Antonella Fantoni, Alice Salvadori, Aleksandr Ovsianikov, Robert Liska, Stefan Baudis

{"title":"Highlighting the Influence between Physical and Chemical Cross-Linking in Dynamic Hydrogels for Two-Photon Micropatterning.","authors":"Antonella Fantoni, Alice Salvadori, Aleksandr Ovsianikov, Robert Liska, Stefan Baudis","doi":"10.1021/acs.biomac.5c00062","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00062","url":null,"abstract":"Photolabile hydrogels have gained tremendous interest for a wide range of applications in materials and life sciences. Usually, photodegradability is introduced via chromophores and labile bonds, making such materials intrinsically light sensitive. In recent years, disulfide bonds have emerged as an innovative alternative, as they can be selectively cleaved in the presence of (photo)generated radicals. However, such materials suffer from limited network stability and high swelling as a result of thiol-disulfide metathesis reactions. Herein, we present two strategies to counteract such phenomena by network stabilization either via physical or chemical incorporation of (un)modified gelatin macromers to norbornene-modified poly(vinyl alcohol) networks. Photolabile behavior was introduced by a simple disulfide-containing dithiol cross-linker. Tunable material properties were investigated by means of in situ photorheology, in vitro swelling, and degradation experiments. Finally, we demonstrate an innovative method for localized disulfide cleavage via two-photon micropatterning.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular Adipose Matrix Hydrogel Laden with Adipose-Derived Stem Cell Modulates Macrophage Polarization for Enhanced Full-Thickness Skin Wound Repair. 载脂肪干细胞的细胞外脂肪基质水凝胶调节巨噬细胞极化促进全层皮肤伤口修复。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-05-08 DOI: 10.1021/acs.biomac.5c00194

Tao He, Qiuwen Tan, Yizhou Huang, Jun Chen, Jie Tan, Chen Zhou, Li Xu, Rong Nie, Qingyi Zhang, Peng Liang, Qing Lv, Hui-Qi Xie

{"title":"Extracellular Adipose Matrix Hydrogel Laden with Adipose-Derived Stem Cell Modulates Macrophage Polarization for Enhanced Full-Thickness Skin Wound Repair.","authors":"Tao He, Qiuwen Tan, Yizhou Huang, Jun Chen, Jie Tan, Chen Zhou, Li Xu, Rong Nie, Qingyi Zhang, Peng Liang, Qing Lv, Hui-Qi Xie","doi":"10.1021/acs.biomac.5c00194","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00194","url":null,"abstract":"Adipose-derived stem cells (ADSC) represent a promising approach for wound healing, while the limited survival rate has restricted their application. To address this, we equipped a hydrogel from acellular porcine adipose tissue (HAPA) with ADSC to fabricate the HAPA + ADSC composite hydrogel. In addition to serving as a carrier for stem cell delivery, the bioactive components of the HAPA hydrogel support immune regulation and tissue repair. In this study, we demonstrated that the HAPA + ADSC composite could effectively modulate macrophage polarization, promote angiogenesis, and regulate extracellular matrix (ECM) deposition and remodeling, thereby substantially accelerating wound healing. Additionally, transcriptomic sequencing analysis indicated that the HAPA + ADSC composite upregulated Nfkbia and Nfkbie to inhibit the nuclear transcription of RelA-p50 heterodimer so that macrophages polarization toward an M1 phenotype is suppressed. The combined effects of ADSC and HAPA hydrogel make it a promising candidate for functional skin wound healing.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifunctional Plasticized Hyaluronic-Acid-Based Nanogel Dressing for Accelerating Diabetic and Nondiabetic Wounds. 多功能增塑透明质酸纳米凝胶敷料加速糖尿病和非糖尿病伤口。

IF 5.5 2区化学

Biomacromolecules Pub Date : 2025-05-08 DOI: 10.1021/acs.biomac.5c00126

Aalaa K Moussa, Heba A Abd El-Rahman, Riham R Mohamed, Demiana H Hanna

{"title":"Multifunctional Plasticized Hyaluronic-Acid-Based Nanogel Dressing for Accelerating Diabetic and Nondiabetic Wounds.","authors":"Aalaa K Moussa, Heba A Abd El-Rahman, Riham R Mohamed, Demiana H Hanna","doi":"10.1021/acs.biomac.5c00126","DOIUrl":"https://doi.org/10.1021/acs.biomac.5c00126","url":null,"abstract":"Diabetic ulcers are associated with oxidative stress, inflammation, decreased synthesis of pro-healing mediators, and impaired vascularization, which convert the wound from acute to chronic and delay healing. An extended duration of wound healing raises the possibility of complications such as infection, sepsis, and even amputation. The objective of this study is the synthesis of a plasticized cross-linked hyaluronic acid (HA)-grafted poly(acrylamide-co-itaconic acid) nanogel as a nontoxic adhesive, swellable, antibacterial wound dressing with good mechanical properties to protect the wound from pathogens and accelerate the healing process, in addition to decreasing oxidative stress and inflammatory cytokines while increasing anti-inflammatory cytokines and angiogenesis. Nanogel H3 with a ratio (AM/IA) (3:1) showed excellent adhesion with good mechanical properties, biocompatibility, swelling, antioxidant, and antibacterial efficiencies. It showed great wound closure in vitro and in vivo with downregulation of inflammatory cytokines, upregulation of anti-inflammatory cytokines, and enhanced angiogenesis in vivo on diabetic and nondiabetic wounds.","PeriodicalId":30,"journal":{"name":"Biomacromolecules","volume":" ","pages":""},"PeriodicalIF":5.5,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0