Jurnal Kedokteran Hewan最新文献_第3页

PREVALENSI DAN FAKTOR RISIKO INFEKSI Toxocara cati PADA KUCING PELIHARAAN DI KOTA BOGOR (Prevalence and Risk Factors of Toxocara cati Infection in Bogor Pet Cats) 茂物宠物猫猫弓形虫感染的患病率及危险因素

Jurnal Kedokteran Hewan Pub Date : 2016-09-04 DOI: 10.21157/J.KED.HEWAN.V10I2.5044

M. Murniati, Etih Sudarnika, Y. Ridwan

引用次数: 3

PENENTUAN KONSENTRASI SODIUM DODECYL SULFATE DALAM PENGENCER RINGER LAKTAT-KUNING TELUR UNTUK PRESERVASI SEMEN AYAM PELUNG (Determination of Sodium Dodecyl Sulfate Concentration in Ringer Lactate-Egg Yolk Extender for Pelung Rooster Semen Preservation) 十二烷基硫酸钠在龙鸡精液保存用林格乳酸蛋清剂中的含量测定

Jurnal Kedokteran Hewan Pub Date : 2016-09-04 DOI: 10.21157/J.KED.HEWAN.V10I2.5091

Nu'man - Hidayat, C. Sumantri, R. Afnan

{"title":"PENENTUAN KONSENTRASI SODIUM DODECYL SULFATE DALAM PENGENCER RINGER LAKTAT-KUNING TELUR UNTUK PRESERVASI SEMEN AYAM PELUNG (Determination of Sodium Dodecyl Sulfate Concentration in Ringer Lactate-Egg Yolk Extender for Pelung Rooster Semen Preservation)","authors":"Nu'man - Hidayat, C. Sumantri, R. Afnan","doi":"10.21157/J.KED.HEWAN.V10I2.5091","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V10I2.5091","url":null,"abstract":"The objective of this experiment was to determine the addition of sodium dodecyl sulfate (SDS) to Ringer Lactate-Egg Yolk (RL-EY) extender on pelung chicken semen preservation. Semen was collected three times a week from three pelung chickens. Collected semen was evaluated macroscopically and microscopically. Only ejaculates of at least 70% sperm motility was then divided into three equal microtubes. Each of them diluted with RL-EY with ratio 90%:10%, then added with 0.00, 0.025, and 0.05% SDS respectively. The liquid semen then stored at 5 °C for 72 hours. Sperm motility and viability were observed every 12 hours. The addition of 0.025% SDS showed higher spermatozoa motility and viability (72.08±1.44% and 80.82±1.30%) which were significantly higher (P<0.05) than 0.00 and 0.05% SDS addition at 24 hours of storage. There was no differences on the spermatozoa motility and viability between 0.00 and 0.05% SDS addition. The decrease of spermatozoa motility and viability was observed in 0.025% SDS addition (4.17±0.56% and 4.65±0.59%, respectively) that significantly lower compared to 0% and 0.05% SDS addition at 24 hours of storage. In conclusion, 0.025% SDS addition in a Ringer Lactate -Egg Yolk extender maintained pelung spermatozoa motility and viability better than 0.00 and 5% SDS addition.","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"10 1","pages":"170-174"},"PeriodicalIF":0.0,"publicationDate":"2016-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67658960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

EFEK SITOTOKSIK HAARLEM OIL TERHADAP HL-60 CELL LINE DAN Steinernema feltiae (Cytotoxic Effect of Haarlem Oil on HL-60 Cell Line and Steinernema feltiae) 哈勒姆油对HL-60细胞株和麻氏斯坦纳氏菌的细胞毒作用

Jurnal Kedokteran Hewan Pub Date : 2016-09-03 DOI: 10.21157/J.KED.HEWAN.V10I2.5038

M. Bahi, C. Jacob, K. Khairan

引用次数: 4

The Activities of Benzopyran (Chromones) and Benzofuranones (Coumaranones) Derivatives against Steinernema feltiae 苯并吡喃(铬酮类)和苯并呋喃酮(香豆素类)衍生物对斯坦奈菌的活性研究

Jurnal Kedokteran Hewan Pub Date : 2016-03-15 DOI: 10.21157/J.KED.HEWAN.V10I1.3368

K. Khairan, M. Bahi, C. Jacob, R. Idroes

引用次数: 0

PEMBERIAN LIMBAH PERKEBUNAN SINGKONG YANG DISUPLEMENTASI SENG DAN KOBALT TERHADAP PERFORMA REPRODUKSI DOMBA BETINA (Utilization of Cassava Plantation Waste Supplemented with Zinc and Cobalton Reproduction Performance of Ewe) （添加锌和钴的木薯种植废弃物的利用对Ewe繁殖性能的影响）

Jurnal Kedokteran Hewan Pub Date : 2016-03-15 DOI: 10.21157/J.KED.HEWAN.V10I1.3379

I. Hernaman, Kundrat Hidajat, Atun Budiman, S. Nurachma

{"title":"PEMBERIAN LIMBAH PERKEBUNAN SINGKONG YANG DISUPLEMENTASI SENG DAN KOBALT TERHADAP PERFORMA REPRODUKSI DOMBA BETINA (Utilization of Cassava Plantation Waste Supplemented with Zinc and Cobalton Reproduction Performance of Ewe)","authors":"I. Hernaman, Kundrat Hidajat, Atun Budiman, S. Nurachma","doi":"10.21157/J.KED.HEWAN.V10I1.3379","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V10I1.3379","url":null,"abstract":"The study aimed to evaluate the utilization of cassava plantation waste suplemented with zinc and cobalt on ewe reproduction performance. Six pregnacy ewes were allocated at experimentrations, i.e. K1: control (50% fresh grass + 50% concentrate) and K2: 50% fresh grass + 50% cassava plantation waste + 30 ppm zinc + 5 ppm cobalt. The results showed that the concentration of zinc and cobalt in blood of ewe in group K1 and K1 were 7.03 vs 8.22 ppm and 0.48 vs 0.44 ppm, respectively, while in faeces were 147,72 vs 158.59 ppm and 8.3 vs 9.8 ppm. In addition, the concentration of alkaline phosphatase and progresterone in blood were 116 vs 196 u/l and 0.56 vs 0.59 mg/ml, respectively. The average of offsprings weight on 24 day postpartum in K1 and K2 was 3.48 vs 3.16kg. In conclusion, cassava plantation waste could be used as feed for pregnancy and lactating ewe. Supplementation of 30 ppm zinc is efectively increase the concentration of zinc and alkaline phosphatase in blood . Key words: cobalt, reproduction, cassava, zinc, ewe","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"29 1","pages":"91-94"},"PeriodicalIF":0.0,"publicationDate":"2016-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67658340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PENGARUH PEMBERIAN AKAR PASAK BUMI (Eurycoma longifolia Jack.) TERHADAP KERUSAKAN ORGAN HATI MENCIT BUNTING The Effect of Eurycoma longifolia Jack. Roots on Liver Damage of Pregnant Mice 长叶桔梗的药效。孕鼠肝脏损伤的根源

Jurnal Kedokteran Hewan Pub Date : 2016-03-15 DOI: 10.21157/J.KED.HEWAN.V10I1.3366

Ruqiah Ganda Putri Panjaitan, M. Masriani, Z. Zulfan

{"title":"PENGARUH PEMBERIAN AKAR PASAK BUMI (Eurycoma longifolia Jack.) TERHADAP KERUSAKAN ORGAN HATI MENCIT BUNTING The Effect of Eurycoma longifolia Jack. Roots on Liver Damage of Pregnant Mice","authors":"Ruqiah Ganda Putri Panjaitan, M. Masriani, Z. Zulfan","doi":"10.21157/J.KED.HEWAN.V10I1.3366","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V10I1.3366","url":null,"abstract":"This study aims to find out the eeffect of Eurycoma longifolia Jack. roots on the liver damage of pregnant mice. This research used Balb/c strain mice, 2-3 month, with the weight ranging of 27-33 gram. Samples were divided into three treatment groups consisted of three mice. Group I (KI) was negative control (placebo), only treated with 0.06 ml/20 g bw of distilled water, group II (KII) was administered with roots of Eurycoma longifolia Jack extract at dose of 14 mg/20 g bw, and group III (KIII, positive control) was administered with Schizandrae at the dose 0.06 mg/20 g bw. Treatment were done for 7 consecutive days in pregnancy period. On day 8 th , blood samples were collected to examine the level of alanine transaminase (AST) and aspartate transaminase (ALT) enzymes in serum and liver organ were collected to observe the histopathological changes. The average of ALT and AST enzymes level on K1, K2, and K3 were 18.34+0.28, 19.68+0.15, and 19.20+0.08 U/l; and 21.79+0.26, 23.42+0.41, and 22.23+0.52 U/l. The histopathological examination results showed that the administration of E. longifolia Jack roots extract and Schizandrae reveal the karyomegali and fatty change of hepatocytes. It is concluded that the administration of E. longifolia Jack roots extract at the dose of 14 mg/20 g bw could decrease the liver function of pregnant mice. Key words: Eurycoma longfolia Jack. root, pregnancy period, liver histopathological studies","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"10 1","pages":"28-31"},"PeriodicalIF":0.0,"publicationDate":"2016-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67657989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

POTENSI TRANSDIFERENSIASI SEL FIBROBLAS MENJADI SEL SARAF SECARA IN VITRO (Transdifferentiation Potency of Fibroblast Cell to Neuron Cell in Vitro) 成纤维细胞向神经细胞的体外转分化能力

Jurnal Kedokteran Hewan Pub Date : 2016-03-15 DOI: 10.21157/J.KED.HEWAN.V10I1.3370

E. M. Kaiin, I. Djuwita

{"title":"POTENSI TRANSDIFERENSIASI SEL FIBROBLAS MENJADI SEL SARAF SECARA IN VITRO (Transdifferentiation Potency of Fibroblast Cell to Neuron Cell in Vitro)","authors":"E. M. Kaiin, I. Djuwita","doi":"10.21157/J.KED.HEWAN.V10I1.3370","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V10I1.3370","url":null,"abstract":"The aim of this study was to examine the potency of fibroblast cells transdifferentiated to neuron cells in vitro. Newborn rat neuron conditioned medium (NBRN CM) was collected from neuron cells cultured with mDMEM without serum for 48 hours. Fibroblast cells were collected from fetal rat muscle treated with trypsin. Fibroblast cells were culture with 3 kind of culture medium: mDMEM + 0.01 mM β-mercaptoethanol; mDMEM + 50% NBRN-CM and mDMEM + 0.01 mM β-mercaptoethanol + 50% NBRN CM . As control, cells was cultured with mDMEM +10% newborn calf serum (NBCS). The addition of NBRN CM into culture medium resulted in 12.97% newborn cells in fibroblast culture medium passage I. Newborn rat neuron conditioned medium in fibroblast culture medium resulted 12.97% neuron cells at passage 1. The percentage was increased (14.60%) when β- mercaptoethanol added into medium. The same result was found at passage 3 (12.67%; 13.17%). It showed that fibroblast cells has potency to transdifferentiated into neuron cells when cultured with NBRN CM. Further research is needed to know the fibroblast transdifferentiation potency . Key words: fibroblast, transdifferentiation, conditioned medium, neuron cells, in vitro","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"10 1","pages":"49-53"},"PeriodicalIF":0.0,"publicationDate":"2016-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67657783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RESISTENSI ANTIBIOTIK DAN DETEKSI GEN PENGODE METHICILLIN RESISTANT Staphylococcus aureus (MRSA) ISOLAT BROILER DI WILAYAH YOGYAKARTA (Resistance of Antibiotics and Detection of Gene Encoding Methicillin Resistant Staphylococcus aureus (MRSA) Isolated from Broiler in Yogyakarta) 耐甲氧西林金黄色葡萄球菌(MRSA)分离株肉鸡DI WILAYAH YOGYAKARTA(日惹肉鸡耐甲氧西林金黄色葡萄球菌(MRSA)的耐药性及基因检测)

Jurnal Kedokteran Hewan Pub Date : 2016-03-15 DOI: 10.21157/J.KED.HEWAN.V10I1.3360

Khusnan Khusnan, Dwi Kusmanto, Mitra Slipranata

引用次数: 4

HUBUNGAN ANTARA AKTIVASI MAKROFAG DENGAN KADAR INTERLEUKIN-6 DAN ANTIBODI TERHADAP Salmonella typhi PADA MENCIT (Relationship between the Macrophage Activity with Interleukin-6 Levels and Titers of Antibodies against Salmonella typhi) HUBUNGAN ANTARA AKTIVASI MAKROFAG DENGAN KADAR白细胞介素-6和抗体TERHADAP伤寒沙门氏菌PADA MENCIT

Jurnal Kedokteran Hewan Pub Date : 2016-03-15 DOI: 10.21157/J.KED.HEWAN.V10I1.3359

I. Besung, N. Astawa, K. Suata, N. K. Suwiti

{"title":"HUBUNGAN ANTARA AKTIVASI MAKROFAG DENGAN KADAR INTERLEUKIN-6 DAN ANTIBODI TERHADAP Salmonella typhi PADA MENCIT (Relationship between the Macrophage Activity with Interleukin-6 Levels and Titers of Antibodies against Salmonella typhi)","authors":"I. Besung, N. Astawa, K. Suata, N. K. Suwiti","doi":"10.21157/J.KED.HEWAN.V10I1.3359","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V10I1.3359","url":null,"abstract":"This study aims to determine the relationships between macrophage activities and interleukin-6 (IL-6 levels), and titer antibody to Salmonella typhi (S. typhi). A total of 32 mice were infected intraperitoneally with 10 5 cells/ml of S. typhi. The activities of macrophages were observed a day after the infection, while the observations of the levels of IL-6 and titer antibody employing enzyme-linked immunosorbent assay (ELISA) method were conducted after one and two weeks of infection. The results showed that the average activities of macrophages after infected by S. typhi was 77.84±11.23 sel, and the levels of IL-6 and antibody titers were higher (159.55±90.11 pg and 56.87±23.32 IU respectively). The macrophage activities were significantly increased (P<0.01) with increasing the level of IL-6 to Y= -0.317x 2 + 0.0033x 2 +494.09 with the relatedness (r)= 0.89 and the antibody titers to Y= -0.020x 2 + 0.00031x + 29.083 with the relatedness (r)= 0.95 . Key words: macrophage activity, IL-6, antibody titers, Salmonella typhi","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"10 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2016-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67657838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

MEASUREMENT OF SERUM TESTOSTERONE IN KACANG GOAT BYUSING ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TECHNIQUE: THE IMPORTANCE OF KIT VALIDATION (Pengukuran Testosteron Serum Kambing Kacang dengan Teknik Enzyme-Linked Immunosorbent Assay (ELISA): Pentingnya Validasi Kit) 酶联免疫吸附法(ELISA)测定卡苍山羊血清睾酮:试剂盒验证的重要性(企鹅睾酮血清Kambing卡苍山登干Teknik酶联免疫吸附法(ELISA): Pentingnya验证试剂盒)

Jurnal Kedokteran Hewan Pub Date : 2016-03-15 DOI: 10.21157/J.KED.HEWAN.V10I1.3367

Gholib G, S. Wahyuni, Okta Hilda Kadar, M. Adam, T. M. Lubis, Azhar A, M. Akmal, T. Siregar, T. Armansyah, T. P. Nugraha

{"title":"MEASUREMENT OF SERUM TESTOSTERONE IN KACANG GOAT BYUSING ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TECHNIQUE: THE IMPORTANCE OF KIT VALIDATION (Pengukuran Testosteron Serum Kambing Kacang dengan Teknik Enzyme-Linked Immunosorbent Assay (ELISA): Pentingnya Validasi Kit)","authors":"Gholib G, S. Wahyuni, Okta Hilda Kadar, M. Adam, T. M. Lubis, Azhar A, M. Akmal, T. Siregar, T. Armansyah, T. P. Nugraha","doi":"10.21157/J.KED.HEWAN.V10I1.3367","DOIUrl":"https://doi.org/10.21157/J.KED.HEWAN.V10I1.3367","url":null,"abstract":"This study was conducted to validate a commercial testosterone enzyme-linked immunosorbent assay (ELISA) kits (DRG EIA-1559) in analytic and biological manner for measuring serum testosterone concentrations in kacang goats. This study used 18 healthy kacang goats, six bucks (>2 years), six kids ( 2 years). Blood samples were collected from jugular vein and prepared as serum. Two validation tests were performed, an analytical validation comprises a parallelism, accuracy, precision and sensitivity and a biological validation by comparing testosterone concentration from bucks, kids, and does. Testosterone concentrations were measured using ELISA technique. Data of analytical validation were analyzed descriptively and test of equality of slope was performed to see the parallelism between samples and standard curves. Analysis of variance (ANOVA) was used for biological validation data. Results of parallelism showed that sample curve was parallel to the standard curve. Accuracy, precision (% CV of intra-and inter-assay) and sensitivity of the assay were 99.65±4.27%, <10%, <15% and 0.083 ng/ml, respectively. Results of biological validation showed that the assay used were accurately measured testosterone which testosterone concentrations in bucks were significantly higher compared to kids and does (P<0.05). In conclusion, a commercial testosterone ELISA kits (DRG EIA-1559) is a reliable assay for measuring serum testosterone concentration in kacang goats. Key words: analytical and biological validations, ELISA, testosterone, kacang goat","PeriodicalId":30999,"journal":{"name":"Jurnal Kedokteran Hewan","volume":"10 1","pages":"32-36"},"PeriodicalIF":0.0,"publicationDate":"2016-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67658032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14