RESISTENSI ANTIBIOTIK DAN DETEKSI GEN PENGODE METHICILLIN RESISTANT Staphylococcus aureus (MRSA) ISOLAT BROILER DI WILAYAH YOGYAKARTA (Resistance of Antibiotics and Detection of Gene Encoding Methicillin Resistant Staphylococcus aureus (MRSA) Isolated from Broiler in Yogyakarta)

Jurnal Kedokteran Hewan Pub Date : 2016-03-15 DOI:10.21157/J.KED.HEWAN.V10I1.3360

Khusnan Khusnan, Dwi Kusmanto, Mitra Slipranata

引用次数: 4

Abstract

The aims of the research were to evaluate resistance of Staphylococcus aureus against several antibiotics and to detect the gene encoding of methicillin resistant Staphylococcus aureus (MRSA) in broiler. Twenty three Staphylococcus aureus isolated from infected broiler in Yogyakarta were used. Genotypic identifications were done based on the occurrence of gene nuc and 23SrRNA. The resistance assay to several antibiotics revealed that Staphylococcus aureus were resistant to penicillin 78% (18/23), doxycyclin 56% (15/23), gentamicin 26% (6/23), tetracyclin 22% (5/23), erythromycin 13% (3/23), and methicillin 9% (2/23). Detection of gene encoding MRSA on 23 isolats of S. aureus showed that 8 (34.8%) isolates contain gene mecA. Key words: Staphylococcus aureus, broiler, resistant, methicillin, mecA

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耐甲氧西林金黄色葡萄球菌(MRSA)分离株肉鸡DI WILAYAH YOGYAKARTA(日惹肉鸡耐甲氧西林金黄色葡萄球菌(MRSA)的耐药性及基因检测)

本研究的目的是评价金黄色葡萄球菌对几种抗生素的耐药性，并检测肉仔鸡耐甲氧西林金黄色葡萄球菌(MRSA)的基因编码。采用从日惹市感染肉鸡中分离的23株金黄色葡萄球菌。根据基因nuc和23SrRNA的出现情况进行基因型鉴定。结果表明，金黄色葡萄球菌对青霉素的耐药率为78%(18/23)，对强力霉素的耐药率为56%(15/23)，对庆大霉素的耐药率为26%(6/23)，对四环素的耐药率为22%(5/23)，对红霉素的耐药率为13%(3/23)，对甲氧西林的耐药率为9%(2/23)。对23株金黄色葡萄球菌进行MRSA编码基因检测，8株(34.8%)含有mecA基因。关键词:金黄色葡萄球菌，肉鸡，耐药，甲氧西林，mecA

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Jurnal Kedokteran Hewan

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24 weeks