Environment International最新文献

{"title":"Temperature extremes and human health in Cyprus: Investigating the impact of heat and cold waves","authors":"Fragkeskos Kekkou ,&nbsp;Theo Economou ,&nbsp;Georgia Lazoglou ,&nbsp;Christina Anagnostopoulou","doi":"10.1016/j.envint.2025.109451","DOIUrl":"10.1016/j.envint.2025.109451","url":null,"abstract":"<div><div>In Mediterranean countries like Cyprus, extreme temperatures and especially heatwaves during summer months are increasingly common, leading to heightened bio-climatic stress. While coldwaves are less frequent and understudied in this region, they still pose significant health risks. This study utilizes temperature data from ERA5–Land in conjunction with mortality and hospitalization data from Cyprus. Employing statistical machine learning methods such as Distributed Lag Models (DLMs) and Generalized Additive Models (GAMs), mortality and hospitalization risks for major health concerns in Cyprus, along with attributable deaths and hospital admissions associated with temperature fluctuations and extreme events over two seasons, were estimated. This study also examines temperature trends over the past four decades in Cyprus, noting a significant increase in both maximum and minimum temperatures as well as an increase in extreme high-temperature days and a decrease in extreme low-temperature days. For mortality, elevated risks were identified at very high temperatures, peaking at shorter lags with up to a 7.5% above-average risk, which further increased to 14.3% during heatwave events in the warm season. Conversely, mortality risks at very low temperatures during the cold season reached 4.2% particularly for longer lag days. Hospitalization risks were notably higher during periods of cold temperatures. Importantly, our findings confirm that cold-related mortality exceeds heat-related mortality in absolute terms. However, during heatwave days, the rate of increase in attributable deaths is five times higher compared to typical days in the warm season, whereas for cold days it is about twice as high as on non-cold days.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"199 ","pages":"Article 109451"},"PeriodicalIF":10.3,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143862691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic insights into the cardiovascular effects of acute heat exposure: A multi-omics analysis based on a randomized crossover trial","authors":"Xinlei Zhu ,&nbsp;Xihao Du ,&nbsp;Yixiang Zhu ,&nbsp;Lu Zhou ,&nbsp;Cong Liu ,&nbsp;Yue Niu ,&nbsp;Qingli Zhang ,&nbsp;Yixuan Jiang ,&nbsp;Haidong Kan ,&nbsp;Renjie Chen","doi":"10.1016/j.envint.2025.109495","DOIUrl":"10.1016/j.envint.2025.109495","url":null,"abstract":"<div><div>Heat exposure has been identified as a significant contributory factor in the development of cardiovascular diseases, but the biological mechanisms are not yet fully elucidated. We conducted a randomized crossover trial in healthy adults in Shanghai, China. Each subject was alternatively exposed to moderate temperature (22 °C) and elevated temperature (32 °C) in a chamber for 2 h in random order. Blood pressure (BP) and arterial stiffness were measured before and after each exposure session. Genome-wide DNA methylation, untargeted serum proteomics, metabolomics, and 15 targeted serum biomarkers were analyzed. Linear mixed-effects models were used to analyze the data. The pathway enrichment was performed at the ingenuity pathway analysis platform. The network-based xMWAS analysis was further conducted. A total of 30 participants (15 males and 15 females) completed the trial, with an average age of 21.7 ± 1.5 years. Heat exposure was associated with higher pulse wave velocity (4.4 %, 95 % CI: 0.2, 6.8), augmentation index normalized to 75 bpm heart rate (190.7 %, 95 % CI: 19.0, 362.3) and reflection magnitude (35.9 %, 95 % CI: 12.3, 59.5). Exposure to heat was significantly associated with changes in 2 biomarkers on systemic inflammation, 2 on oxidative stress, 1 on coagulation, 2 on lipid metabolism, and 2 on atherosclerotic alterations. Multi-omics analyses indicated heat-induced perturbations in pathways were mostly related to systemic inflammation, oxidative stress, coagulation, and lipid metabolism disorder. Acute heat exposure might impair cardiovascular function and promote multiple adverse biological processes, especially those related to atherosclerosis progression and increase of plaque instability.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"199 ","pages":"Article 109495"},"PeriodicalIF":10.3,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143862693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Effects of radiofrequency electromagnetic field (RF-EMF) exposure on male fertility: A systematic review of experimental studies on non-human mammals and human sperm in vitro” [Environ. Int. 185 (2024) 108509]","authors":"Eugenia Cordelli, Lucia Ardoino, Barbara Benassi, Claudia Consales, Patrizia Eleuteri, Carmela Marino, Maurizio Sciortino, Paola Villani, Martin H. Brinkworth, Guangdi Chen, James P. McNamee, Andrew W. Wood, Lea Belackova, Jos Verbeek, Francesca Pacchierotti","doi":"10.1016/j.envint.2025.109449","DOIUrl":"https://doi.org/10.1016/j.envint.2025.109449","url":null,"abstract":"The authors of the article with the title “Effects of radiofrequency electromagnetic field (RF-EMF) exposure on male fertility: A systematic review of experimental studies on non-human mammals and human sperm <em>in vitro</em>” regret to report a few inconsistencies detected in the article after its publication, due to errors that inadvertently occurred in the extraction of the original data and in the misfiling of one study for its RoB assessment before the elaboration of the forest plots.","PeriodicalId":308,"journal":{"name":"Environment International","volume":"33 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping chemicals across EU’s legal frameworks towards a ‘one substance, one assessment’ approach","authors":"Mathilda Andreassen, Christina Rudén, Marlene Ågerstrand","doi":"10.1016/j.envint.2025.109460","DOIUrl":"https://doi.org/10.1016/j.envint.2025.109460","url":null,"abstract":"Chemicals in the EU are mainly regulated based on their intended use. Each legal framework consists of requirements and guidance for hazard- and risk assessment, along with the associated decision processes e.g., registration or authorisation of chemicals for market access in the EU. As a single chemical may have multiple uses, it may be assessed under more than one framework, potentially leading to different assessment outcomes. To address this, the European Commission has introduced the ‘one substance, one assessment’ approach as part of the Chemicals Strategy for Sustainability. The aims of the approach include streamlining risk assessment processes and reducing duplication of work in assessing the same chemical. This study aimed to map the scope of chemicals subject to assessment in multiple legal frameworks and to illustrate the importance of coordination and communication in chemical assessment processes. This was achieved by identifying chemicals that are either registered or have received specific approval for the EU market, and analysing their presence in different legal frameworks. Our findings showed that almost one-tenth of the substances identified were listed under more than one framework. However, there was a notable lack of coherent chemical identifiers available to accurately identify chemicals across the frameworks. Additionally, we identified the presence of phthalates, bisphenols and PFAS in EU frameworks to illustrate how a group-based approach to chemical assessment could be applied across different legal frameworks.","PeriodicalId":308,"journal":{"name":"Environment International","volume":"2 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of nontarget screening and QSAR modeling to identify novel butachlor transformation products of high priority in soil and water environment","authors":"Feng Chen,&nbsp;Chi Wu ,&nbsp;Lan Zhang,&nbsp;Liangang Mao,&nbsp;Lizhen Zhu,&nbsp;Xingang Liu","doi":"10.1016/j.envint.2025.109491","DOIUrl":"10.1016/j.envint.2025.109491","url":null,"abstract":"<div><div>Butachlor (BTR), a widely used chloroacetanilide herbicide in rice paddies worldwide, has raised concerns due to limited data on its environmental transformation products (TPs) and their ecotoxicological effects. This study systematically investigates BTR’s degradation kinetics, mechanisms, pathways, and ecotoxicity in water and soil through batch tests, integrating Non-Target Screening (NTS) with Quantitative structure–activity relationship (QSAR) modeling. Hydrolysis half-lives (DT₅₀) ranged from 73.2 to 196 days (pH 4–9), while photolysis half-lives were 12.9–18.6 h, indicating enhanced degradation in alkaline and light-exposed conditions. In three representative soils, DT₅₀ values ranged from 22.3 to 38 days, with black soil exhibiting the most rapid degradation. Moreover, anaerobic conditions marginally accelerated degradation in both fluvo-aquic and red soils. Using UPLC-Q-TOF-MS, ten TPs were identified, including three novel ones, and four (TP191, TP225, TP277, TP293) were confirmed with synthetic standards. A probable transformation mechanism was suggested based on the identified TPs and Density Functional Theory (DFT) based calculations. Mechanistically, BTR degradation in soil primarily involves N-demethylation, N-debutoxylation and hydrolysis, whereas in water it proceeds through a more diverse array of pathways – including dechlorination, hydroxylation, dehydrogenation, N-debutoxylation, cyclization, hydrolysis, dehydration, and oxidation. Finally, Ecotoxicity modeling showed certain TPs, particularly TP277, had “extreme” acute toxicity to <em>Daphnia magna</em>, while TP277 and TP293 posed higher chronic toxicity risks to <em>Danio rerio</em> than the parent compound. These findings highlight the ecological risks of BTR TPs and advocate for their inclusion in environmental risk assessments to safeguard aquatic ecosystems.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"199 ","pages":"Article 109491"},"PeriodicalIF":10.3,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population-specific exposure risks from parabens and antimicrobials in cosmetics and personal care products: Insights from Korean usage patterns","authors":"Sori Mok ,&nbsp;Jae-Eun Lim ,&nbsp;Miyoung Lim ,&nbsp;Ji Young Park ,&nbsp;Kiyoung Lee ,&nbsp;Hyo-Bang Moon","doi":"10.1016/j.envint.2025.109476","DOIUrl":"10.1016/j.envint.2025.109476","url":null,"abstract":"<div><div>The use of cosmetics and personal care products (CPCPs) is a significant source of human exposure to endocrine disrupting chemicals (EDCs). However, few studies have investigated EDC exposure through CPCP use based on realistic exposure factors of diverse population groups. In this study, 13 parabens and two antimicrobial agents were measured in 261 CPCPs categorized as leave-on, rinse-off, and baby care products. Methylparaben and propylparaben were the most prevalent parabens. Triclocarban was frequently found in rinse-off products, while triclosan was rarely detected, likely due to regulatory measures. Population-specific exposure assessments were conducted using measured EDC concentrations and exposure factors derived from surveys of 1,001 adults, 322 teenagers, and 719 mother-infant pairs in Korea. Dermal exposure doses (DEDs) of parabens were estimated at 11.4, 25.8, 25.1 and 0.03 µg/kg/day for males, females, mothers, and infants, respectively. For antimicrobial agents, DEDs were 0.20, 0.28, and 0.98 µg/kg/day for males, females, and mothers, respectively. Females had higher DEDs of parabens than males in both adults and teenagers, and mothers had higher DEDs of triclocarban. These findings emphasized the need to consider population-specific characteristics in exposure assessments. High-exposure scenarios resulted in DEDs 4–5 times higher than general-exposure scenarios. Skin care products, sunscreens, body and hand lotions were major contributors to paraben exposure, highlighting the need for targeted management to mitigate exposure from these products. This study provides realistic exposure data that can serve as a valuable reference for managing CPCP ingredients, particularly parabens and antimicrobial agents.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"199 ","pages":"Article 109476"},"PeriodicalIF":10.3,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gestational exposure to particulate matter from urban wildfires is associated with changes in circulating oxylipins but not flame retardants 7 to 13 months post-exposure","authors":"Eunyoung Park, Qing Shen, Zhichao Zhang, Claire E. O’Brien, Amanda J. Goodrich, Elizabeth E. Angel, Irva Hertz-Picciotto, Daniel J. Tancredi, Sean Raffuse, Deborah H. Bennett, Rebecca J. Schmidt, Ameer Y. Taha","doi":"10.1016/j.envint.2025.109468","DOIUrl":"https://doi.org/10.1016/j.envint.2025.109468","url":null,"abstract":"Exposure to fine particulate matter (PM_2.5) from wildfire smoke has been linked to immune dysregulation underlying multiple health conditions, but data on the long-term effects of these exposures during gestation are lacking. Smoke PM_2.5 from wildfires occurring in urban areas is of particular concern because it can carry persistent chemicals within household furniture or soil, as well as polyaromatic hydrocarbons (PAHs) from combusted materials. The present study investigated the long-term associations between wildfire PM_2.5 and serum polybrominated diphenyl ethers (PBDEs), polychlorinated biphenyls (PCBs), PAHs and lipid mediators (i.e., oxylipins) involved in immune regulation in participants from the B-SAFE (Bio-Specimen Assessment of Fire Effects) study, which enrolled women pregnant during or shortly after the 2017 Tubbs Fire in California (n = 140). Serum samples were collected and assayed 7 to 13 months post-exposure, at which point 20 women were still pregnant and 120 women were postpartum. Adjusted linear regression models revealed a significant positive association between increasing PM_2.5 (μg/m³) exposure and serum concentrations of benzo[k]fluoranthene, a PAH (β = 0.866, <em>P</em> = 0.0403, [95 %CI: 0.0389, 1.69]). No associations were observed between PM_2.5 exposure and serum PBDEs, PCBs or other PAHs. Increased exposure to PM_2.5 was associated with lower serum concentrations of lipoxygenase (LOX)-derived free oxylipins and increased concentrations of LOX-derived oxylipins esterified to circulating lipids. These findings provide new evidence of long-term effects of gestational wildfire PM_2.5 exposure on the turnover of oxylipins involved in inflammation and vasoconstriction via the LOX pathway. Additional studies are warranted to better understand the impact of these changes on maternal and child health.","PeriodicalId":308,"journal":{"name":"Environment International","volume":"137 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial effects, and selection for AMR by non-antibiotic drugs in a wastewater bacterial community","authors":"April Hayes, Lihong Zhang, Edward Feil, Barbara Kasprzyk-Hordern, Jason Snape, William H Gaze, Aimee K Murray","doi":"10.1016/j.envint.2025.109490","DOIUrl":"https://doi.org/10.1016/j.envint.2025.109490","url":null,"abstract":"Antimicrobial resistance (AMR) is a major threat to human, animal, and crop health. AMR can be directly selected for by antibiotics, and indirectly co-selected for by biocides and metals, at environmentally relevant concentrations. Some evidence suggests that non-antibiotic drugs (NADs) can co-select for AMR, but previous work focused on exposing single model bacterial species to predominately high concentrations of NADs. There is a significant knowledge gap in understanding a range of NAD concentrations, (including lower µg/L concentrations found in the environment) on mixed bacterial communities containing a diverse mobile resistome. Here, we determined the antimicrobial effect and selective potential of diclofenac, metformin, and 17-β-estradiol, NADs that are commonly found environmental pollutants in a complex bacterial community using a combination of culture based, metagenome, and metratranscriptome approaches. We found that diclofenac, metformin, and 17-β-estradiol at 50µg/L, 26µg/L, and 24µg/L respectively, significantly reduced growth of a bacterial community although only 17-β-estradiol selected for an AMR marker using qPCR (from 7µg/L to 5400µg/L). Whole metagenome sequencing indicated that there was no clear selection by NADs for antibiotic resistance genes, or effects on community composition. Additionally, increases in relative abundance of some specific metal resistance genes (such as <em>arsB</em>) were observed after exposure to diclofenac, metformin, and 17-β-estradiol. These results indicate that environmentally relevant concentrations of NADs are likely to affect community growth, function, and potentially selection for specific metal resistance genes.","PeriodicalId":308,"journal":{"name":"Environment International","volume":"103 1","pages":""},"PeriodicalIF":11.8,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating inhalation risks and toxicological impacts of lithium-ion battery thermal runaway emissions","authors":"Maureen Meister ,&nbsp;Shaligram Sharma ,&nbsp;Xiaojia He ,&nbsp;Patrick S. Chepaitis ,&nbsp;Taryn Waddey ,&nbsp;Mark Wilson ,&nbsp;Vinay Premnath ,&nbsp;Judith Jeevarajan ,&nbsp;Marilyn Black ,&nbsp;Christa Wright","doi":"10.1016/j.envint.2025.109466","DOIUrl":"10.1016/j.envint.2025.109466","url":null,"abstract":"<div><div>The occurrence of thermal runaway (TR) events continues to rise as the need for lithium-ion batteries (LIB) for energy storage increases. However, the inhalation risks associated with LIB TR events remain widely unknown. The objective of this study was to evaluate the impact of LIB TR particulate emission exposures on primary small airway epithelial cells (SAEC). TR was triggered by subjecting lithium-ion cells to thermal abuse at different states of charge (SOC). Two different battery cathode chemistry compositions, namely, nickel manganese cobalt (NMC) or lithium iron phosphate (LFP) were evaluated. Aerosol monitoring and sampling instrumentation were employed followed by physicochemical particle characterization and inhalation dosimetry modeling. SAEC were treated with TR particulate emission extracts for 24 h and 7 days at doses representing a cumulative 1- and 5-year inhalation exposure. Following treatment, cellular viability, reactive oxygen species (ROS) production, and protein expression of DNA damage and epithelial mesenchymal transition (EMT) markers were assessed. TR particulate emissions consisted of ultrafine particles containing a variety of heavy metals. Cellular senescence was induced by NMC-derived TR extracts, but not LFP-derived TR extracts. SAEC treated with the 5-year dose of NMC-derived TR extract, induced significant ROS production. In cells treated with NMC-derived TR extract, regulators of DNA repair and cell cycle arrest were perturbed. Oxidative stress subsequently induced EMT, as SAEC treated with NMC-derived TR particulate emissions reduced E-cadherin expression and upregulated Fascin and Vimentin expression. This study reveals the respiratory implications of TR particulate emissions and the role of battery chemistry.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"199 ","pages":"Article 109466"},"PeriodicalIF":10.3,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive assessment of non-indigenous species requires the combination of multi-marker eDNA metabarcoding with classical taxonomic identification","authors":"Stefano Varrella ,&nbsp;Silvia Livi ,&nbsp;Cinzia Corinaldesi ,&nbsp;Luca Castriota ,&nbsp;Teresa Maggio ,&nbsp;Pietro Vivona ,&nbsp;Massimo Pindo ,&nbsp;Sebastiano Fava ,&nbsp;Roberto Danovaro ,&nbsp;Antonio Dell’Anno","doi":"10.1016/j.envint.2025.109489","DOIUrl":"10.1016/j.envint.2025.109489","url":null,"abstract":"<div><div>In marine environment, non-indigenous species (NIS) can alter natural habitats and cause biodiversity loss with important consequences for ecosystems and socio-economic activities. With more than 1000 NIS introduced over the last century, the Mediterranean Sea is one of the most threatened regions worldwide, requiring an early identification of newly entered alien species for a proper environmental management. Here, we carried out environmental-DNA (eDNA) metabarcoding analyses, using multiple molecular markers (i.e., <em>18S rRNA</em>, <em>COI</em>, and <em>rbcL</em>) and different genetic databases (i.e., NCBI, PR2, SILVA, MIDORI2, MGZDB, and BOLD), on seawater and sediment samples collected on a seasonal basis in three Mediterranean ports located in the North Adriatic, Ionian and Tyrrhenian Sea to identify marine species, and particularly NIS. The use of the multi-marker eDNA metabarcoding allowed the identification of a higher number of species compared to the morphological analyses (1484 vs. 752 species), with a minor portion of species shared by both approaches. Overall, only 4 NIS were consistently identified by both morphological and molecular approaches, whereas 27 and 17 NIS were exclusively detected by using eDNA metabarcoding and classical taxonomic analyses, respectively. The eDNA metabarcoding allowed also identifying the genetic signatures of 5 NIS never reported in the Italian waters. We conclude that eDNA metabarcoding can represent a highly sensitive tool for the early identification of NIS, but a comprehensive census of the NIS requires the combination of molecular and morphological approaches.</div></div>","PeriodicalId":308,"journal":{"name":"Environment International","volume":"199 ","pages":"Article 109489"},"PeriodicalIF":10.3,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143853860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}