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Assessment of the current hydrochemical state of some fishponds of Kyiv region 基辅地区一些鱼塘的水化学现状评估
Ribogospodars''ka Nauka Ukraini Pub Date : 2023-09-29 DOI: 10.15407/fsu2023.03.020
L. Dragan, N. Mikhailenko, T. Bersan
{"title":"Assessment of the current hydrochemical state of some fishponds of Kyiv region","authors":"L. Dragan, N. Mikhailenko, T. Bersan","doi":"10.15407/fsu2023.03.020","DOIUrl":"https://doi.org/10.15407/fsu2023.03.020","url":null,"abstract":"Purpose. To assess the quality of the hydrochemical regime of fishponds, the territory of which suffered massive missile strikes or was directly a combat zone or close to the front line. Methodology. Chemical parameters of water including the content of ammonium nitrogen, nitrites, nitrates, phosphates and total iron were measured by the photometric method, while chloride concentration of chlorides by the argentometric titration method. The hydrogen indicator (pH) was determined electrometrically, and sulfate content and mineralization by the gravimetric method. All the above-mentioned methods are generally accepted in the comprehensive assessment of the ecological state of fishponds. Findings. The total amount of mineral salts dissolved in water was studied and analyzed in laboratory conditions, namely: the concentrations of calcium, magnesium, chlorine, bicarbonate ions and sulfate ions. The determined permanganate oxidizability in the investigated ponds exceeded the normative values by 72, 49, 31, and 42%, respectively. The concentration of nitrates ranged from 19.96 to 6.34 mg N/dm3, nitrites from 0.33 to 0.11 mgN/dm3, which indicates the intensity of decomposition of organic substances and indicates a certain degree of contamination of ponds. The amount of total iron in the studied ponds ranged from 1.98 to 1.17 mgFe/dm3, respectively. A slight increase in mineral phosphorus in ponds No. 1 and No. 2 was established by 31 and 26%, respectively, which can probably be related to both the decomposition of zooplankton and the input of pollutants from the outside from the surrounding areas. The obtained results of the laboratory analysis of the chemical composition of water samples as well as their comparison with the normative values for water bodies used for aquaculture provide a basis for assessing the current hydrochemical state of the investigated ponds of Kyiv region Originality. For the first time, a hydrochemical analysis of fishponds in Kyiv Region was conducted, the territory of which suffered massive missile strikes or was directly a combat zone or close to the front line. Practical value. The obtained results will contribute to a better understanding of the current ecological state of fishponds in Kyiv region. Keywords: water body, chemical analysis of water, toxic substances, ecological condition.","PeriodicalId":30821,"journal":{"name":"Ribogospodars''ka Nauka Ukraini","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135293937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Black carp (Mylopharyngodon piceus Richardson, 1846). Thematic bibliography 黑鱼(Mylopharyngodon piceus Richardson, 1846)。专题书目
Ribogospodars''ka Nauka Ukraini Pub Date : 2023-09-29 DOI: 10.15407/fsu2023.03.157
Ir. Hrytsynyak, T. Shvets
{"title":"Black carp (Mylopharyngodon piceus Richardson, 1846). Thematic bibliography","authors":"Ir. Hrytsynyak, T. Shvets","doi":"10.15407/fsu2023.03.157","DOIUrl":"https://doi.org/10.15407/fsu2023.03.157","url":null,"abstract":"Purpose. Forming a thematic bibliographic list of English-language publications on molecular-genetic, immunological, physiological-biochemical, as well as ecological and individual biological features of black carp (Mylopharyngodon piceus L.). Methods. The complete and selective methods were applied in the process of the systematic search. The bibliographic core have been consisted of English-language scientific publications from resources available to users of the Institute of Fisheries NAAS Scientific Library. Results. There was composed the thematic list of publications with a total quantity of 89 sources covering the time interval from 1995 to 2023, and highlighting the biological and ecological characteristics of a representative of the Cyprinids, the black carp, as well as considering the issue of its physiological, biochemical, genetic, and immunological features. The literary sources are arranged in alphabetical order by author or title, and described according to DSTU 8302:2015 “Information and documentation. Bibliographic reference. General principles and rules of composition”, with the amendments (code UKND 01.140.40), as well as in accordance with the requirements of APA style — international standard of references. Practical value. The list may be useful for scientists, practitioners, students, whose area of interests includes issues of biological research of Cyprinids, in particular black carp (Mylopharyngodon piceus L.). Key words: black carp, immunology, genetic indicators, biochemical indicators.","PeriodicalId":30821,"journal":{"name":"Ribogospodars''ka Nauka Ukraini","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135294005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Wintering of the eastern subtropical freshwater shrimp (Macrobrachium nipponense de Haan, 1849) in the conditions of the Dnister River basin 东亚热带淡水对虾(Macrobrachium nipponense de Haan, 1849)在德尼斯特河流域条件下的越冬
Ribogospodars''ka Nauka Ukraini Pub Date : 2023-09-29 DOI: 10.15407/fsu2023.03.003
P. Shekk, Yu. Astafurov
{"title":"Wintering of the eastern subtropical freshwater shrimp (Macrobrachium nipponense de Haan, 1849) in the conditions of the Dnister River basin","authors":"P. Shekk, Yu. Astafurov","doi":"10.15407/fsu2023.03.003","DOIUrl":"https://doi.org/10.15407/fsu2023.03.003","url":null,"abstract":"Purpose. To study the characteristics of wintering of Macrobrachium nipponense in natural conditions of the Lower Dniester basin and experimental assessment of the effect of physical condition, soil composition and type of shelter on the survival of wintering shrimps. Methodology. The results of field and experimental studies conducted in 2018–2020 were used in the study. The locations and conditions of mass wintering of shrimps in natural conditions in the lower reaches of the Dniester River were studied. The abundance of wintering individuals was determined by the method of direct counting and their physical condition was analyzed. Shrimps were kept in a RAS at the Laboratory of Aquatic Bioresources and Aquaculture of Odesa State Ecological University, where environmental parameters were maintained as close as possible to the conditions of wintering under natural conditions. In chronic experiments, the selectivity and effect of the composition of natural substrates and the thickness of the soil bed on shrimp survival were investigated. The selection and effectiveness of the shrimp’s use of above-ground shelters of various natural and anthropogenic origin for shrimp wintering was assessed depending on their physical condition (presence or absence of injuries). Shrimp for experiments were caught in October - November in the lower reaches of the Dniester River and the Dniester estuary. The location of mass wintering of shrimps was recorded using a GPS navigator. Findings. It was established that shrimp M. nipponense in natural conditions of the Lower Dniester river winters in aboveground shelters (of natural and anthropogenic origin) or by burrowing into the soil. The shrimp’s choice of aboveground shelters for wintering in natural conditions depended on their physical condition. Injured individuals (lost claws and limbs) preferred easily accessible aboveground shelters (sheaves of grass, flooded snags). Healthy individuals wintered in cracks between plant roots and stones. A significant part of shrimps overwintered in aboveground shelters of anthropogenic origin. The most complex and reliable aboveground shelters of natural origin were occupied by the least damaged shrimp individuals. More than 59% of shrimps are buried in the soil (soil shelters) for wintering. Healthy individuals prefer sandy soils or soils that contain sand. The highest yield of healthy shrimp overwintering was observed on sandy or sandy-gravel soils with a thicker soil layer. The depth of burial of shrimp depended on soil composition. It was the maximum in soils consisting of sand, sand-gravel mixture and chernozem or chernozem with inclusions of gravel. The survival of wintering individuals was inversely dependent on the depth of burial. The maximum yield of uninjured individuals from wintering was observed when they were buried in the soil, the minimum — when using aboveground shelters. The share of surviving injured shrimp in soil shelters decreased in proportion to the se","PeriodicalId":30821,"journal":{"name":"Ribogospodars''ka Nauka Ukraini","volume":"2019 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135294401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Modern aspects of pikeperch (Sander lucioperca Linnaeus, 1758) aquaculture (a review) 鲈的现代方面(Sander lucioperca Linnaeus, 1758)水产养殖(综述)
Ribogospodars''ka Nauka Ukraini Pub Date : 2023-09-29 DOI: 10.15407/fsu2023.03.134
O. Polishchuk, M. Simon
{"title":"Modern aspects of pikeperch (Sander lucioperca Linnaeus, 1758) aquaculture (a review)","authors":"O. Polishchuk, M. Simon","doi":"10.15407/fsu2023.03.134","DOIUrl":"https://doi.org/10.15407/fsu2023.03.134","url":null,"abstract":"Purpose. To analyze data from special literature on the current state and basic methods of pikeperch (Sander lucioperca Linnaeus, 1758) aquaculture in Ukraine and other European countries. To determine the differences in technologies for growing this fish species depending on the specifics of the resource provision of aquaculture and regional pecularities of the functioning of fish farming enterprises in European countries. Findings. The review examines the general principles of aquaculture of pikeperch according to various technological schemes, including in the conditions of ponds and industrial-type farms. The peculiarities of the cultivation of different age groups of this fish species in the specific conditions of the development of aquaculture in Ukraine have been clarified. Some peculiarities of the application of treatment and preventive measures used in the process of its cultivation are described. The problems faced by Ukrainian producers of fish products during the organization of feeding of this percid fish are emphasized. Data from domestic and foreign information sources on productivity, methods of wintering and transportation of this member of the European ichthyofauna are summarized. The main prospects for the further development of pikeperch aquaculture in Ukraine and related problems, in particular in the field of technological support, have been determined. Practical Value. The data presented in the review can be used by practitioners of the fish industry and scientists in the field of fisheries in the process of developing pikeperch aquaculture in Ukraine. This will contribute to the improvement of the regulatory and technological base of pond fish farming and increase the efficiency of the development of industrial methods of aquaculture in the farms of different regions of the country. At the same time, the information presented in the review can be used for the training of fisheries specialists during the development of relevant training programs of educational institutions. Keywords: pikepersh(Sander lucioperca Linnaeus, 1758), biocontrol effect, pond aquaculture, industrial aquaculture, recirculation aquaculture system (RAS), reservoirs.","PeriodicalId":30821,"journal":{"name":"Ribogospodars''ka Nauka Ukraini","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135295431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of the genetic variability of pedigree stocks of amur carp (Cyprinus rubrofuscus Lacépède, 1803) 黑龙江鲤鱼(Cyprinus rubrofuscus lac, 1803)系谱种群遗传变异性的评价
Ribogospodars''ka Nauka Ukraini Pub Date : 2023-09-29 DOI: 10.15407/fsu2023.03.086
А. Mariutsa, I. Hrystyniak, Yu. Glushko, Т. Nahorniuk
{"title":"Evaluation of the genetic variability of pedigree stocks of amur carp (Cyprinus rubrofuscus Lacépède, 1803)","authors":"А. Mariutsa, I. Hrystyniak, Yu. Glushko, Т. Nahorniuk","doi":"10.15407/fsu2023.03.086","DOIUrl":"https://doi.org/10.15407/fsu2023.03.086","url":null,"abstract":"Purpose. To study the genetic peculiarities of pedigree tocksof Amur carp and evaluate its genetic variability by analyzing distribution of alleles and genotypes by the specific protein systems and cytogenetic parameters. Methodology. Analysis of polymorphism of protein systems was performed using electrophoresis in polyacrylamide gel. As protein markers to evaluate the genetic structure of Amur carp stocks, the separation of allelic and genotypic frequencies by loci, which encode a number of fish blood proteins, were used: transferrin (TF), albumin (ALB) and esterase (EST, 3.1.1.1). Cytogenetic analysis was performed using the micronucleus test and analysis of apoptosisfrequencies. Collection and processing of biological material of fish as well as statistical processing of the obtained data was performed using generally accepted methods. Findings. A comprehensive analysis of the genetic structure of Amur carp from three farms in Ukraine was carried out by protein systems and cytogenetic markers. The results of the study showed that all pedigree groups of Amur carp were characterized by a deviation towards the predominance of heterozygotes (Fis = from –0.084 to –0.344). A minimal imbalance was observed in the group of carp from the farm “Karpatskyi Vodogray” LLC (Fis = –0.084). The maximum violation of the genetic balance was found in carp from the farm of JSC “Sumyrybhosp” (Fis = –0.308) and in the group from the experimental farm “Veliky Lyubin” (Fis = –0.344). In general, the analysis of the protein systems of fish blood showed a high level of heterogeneity of pedigree stocks of Amur carp from different farms in Ukraine. The analysis of frequencies of cytogenetic parameters of Amur carp from three fish farms showed that fish from fish farms of JSC “Sumyrybhosp” and PFE “Dzherelo” were characterized by a lower frequency of erythrocytes with micronuclei (EMN) (3.3±0.3 ‰), (3.2±0.3 ‰), lymphocytes with micronuclei (LMN) (2.1±0, 2 ‰), (1.9±0.2 ‰), and apoptosis (4.2±0.3‰), (4,3±0,3‰) compared to the group from fish farm “Karpatskyi Vodogray” LLC, where these values were as follows: EMN (4.7±0.3 ‰), LMN (2.4±0.2‰), apoptosis (5.6±0.4‰). These results indicates a lower level of destabilization of chromosomal apparatus of Amur carp from the fish farm JSC “Sumyrybhosp” at the time of the study. Originality. A comprehensive evaluation of the level of variability of the genetic structure of pedigree stocks of Amur carp from different regions of Ukraine was performed for the first time. Practical Value. Pedigree socksof Amur carp have important practical value in selective breeding work with carps, including works for obtaining hybrid lines to increase the resistance of breeding material. Keywords: Amur carp, genetic structure, locus, alleles, genotype, heterozygosity, micronucleus test, cytogenetic analysis.","PeriodicalId":30821,"journal":{"name":"Ribogospodars''ka Nauka Ukraini","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135295367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biological peculiarities of artificial reproduction of sturgeons (Acipenseriformes) (a rеview) 中华鲟人工繁殖的生物学特性(概览)
Ribogospodars''ka Nauka Ukraini Pub Date : 2023-09-29 DOI: 10.15407/fsu2023.03.057
O. Kuzmenko, N. Vovk
{"title":"Biological peculiarities of artificial reproduction of sturgeons (Acipenseriformes) (a rеview)","authors":"O. Kuzmenko, N. Vovk","doi":"10.15407/fsu2023.03.057","DOIUrl":"https://doi.org/10.15407/fsu2023.03.057","url":null,"abstract":"Purpose. To analyze the scientific data of domestic and foreign authors regarding the peculiarities of artificial reproduction of sturgeonss (Acipenseriformes). To investigate the state of study of the phenomenon of polyspermic fertilization in sturgeons and the factors that induce it in the conditions of artificial reproduction and cultivation. Findings. A review analysis of the results of scientific studies by domestic and foreign authors was carried out on the pecularities of artificial reproduction of sturgeons and the phenomenon of polysperm fertilization of their eggs in controlled conditions of aquaculture. It is shown that information on the problems of sturgeon polyspermy and its induction by various factors was practically absent in the available professional literature in recent years. Since during the artificial reproduction of sturgeons, a significant number of embryos have atypical development, which leads to their death even before hatching, it has been suggested that atypical forms of cell division were caused by polyspermy. The conducted studies added to the knowledge about the peculiarities of the structure of the sturgeon egg envelope and the processes that occur during fertilization. Despite the fact that there is a natural mechanism to prevent the possibility of polyspermic fertilization, this phenomenon occurs quite often, causing an impairment of zygote fragmentation at the initial stages of embryogenesis, and therefore a decrease in the yield of larvae. Understaning of the mechanism of blocking polyspermy in sturgeons and the factors that induce it requires further studies. The list of scientific articles includes 61 sources, 58 in English. The published publications mainly cover the period of the last twenty years. Practical value. A review of data from scientific and professional sources on the specified topic will allow a comprehensive approach to the understanding of the peculiarities of artificial reproduction of sturgeons, the phenomenon of polyspermy and increasing the yield of larvae in sturgeon farming. Review material on this topic can be interesting and useful for scientists, practitioners, students whose interests are related to aquaculture and used in the educational process of training specialists in specialty 207 “Aquatic bioresources and aquaculture”. Keywords: aquaculture, sturgeons (Acipen­seriformes), eggs, egg envelope, micropyle, fertilization, cortical reaction, polyspermy, embryos.","PeriodicalId":30821,"journal":{"name":"Ribogospodars''ka Nauka Ukraini","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135294190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Perspectives of artificial reproduction of the vyrezub (Rutilus frisii frisii Nordman, 1840) in Ukraine (a rеview) 乌克兰vyrezub (Rutilus frisii frisii Nordman, 1840)人工繁殖的展望(全景图)
Ribogospodars''ka Nauka Ukraini Pub Date : 2023-09-29 DOI: 10.15407/fsu2023.03.034
V. Gurbyk, A. Mruk, A. Kucheruk
{"title":"Perspectives of artificial reproduction of the vyrezub (Rutilus frisii frisii Nordman, 1840) in Ukraine (a rеview)","authors":"V. Gurbyk, A. Mruk, A. Kucheruk","doi":"10.15407/fsu2023.03.034","DOIUrl":"https://doi.org/10.15407/fsu2023.03.034","url":null,"abstract":"Purpose. The main goal of the thematic review was the analysis and generalization of literary sources on the peculiarities of its biology, technological aspects of artificial reproduction and growing of vyrezub (Rutilus frisii frisii Nordman, 1840) for the restoration of its natural population in water bodies of Ukraine. To evaluate the perspectives of artificial reproduction and growing of vyrezub in aquaculture of Ukraine. Findings. As a result of human impact on river networks of Ukraine, the abundance of vyrezub significantly decreased, it has become a rare and at the same time an endangered species. According to retrospective data, vyrezub previously belonged to the widespread commercial species of the Dnipro and Southern Bug rivers. The biological features of this species have been analyzed, the observance of which can allow obtaining viable fish seeds in aquaculture conditions. The adaptive possibilities of vyrezub to artificial conditions of reproduction have been reviewed. Historical information on the reproduction of this species over the last century has been analyzed. On the territory of Ukraine, first attempts to artificially reproduce vyrezub were carried out by specialists of Ukrcherrybvod. According to the results of studies of Ukrainian scientists, several stages of artificial reproduction of vyrezub have been identified, namely: taking spawners from natural water bodies, obtaining eggs and sperm, incubation of eggs in incubation devices, obtaining 6 g fish seeds, release of viable juveniles into natural water bodies. However, the works on artificial reproduction of vyrezub were not successful, which was the reason for their complete cessation. In particular, this species is promising for growing in polyculture, as it does not create intense trophic competition with most objects of freshwater aquaculture, and as a result of the consumption of mollusks will reduce the likelihood of the spread of some invasive fish diseases. Practical value. The review of information sources on various aspects of the use of vyrezub in aquaculture will contribute to the development of a system of separate links of the technology of its cultivation for the needs of rearing in the conditions of aquaculture enterprises and stocking of internal water bodies with viable juveniles. Key words: vyrezub (Rutilus frisii frisii Nord­man, 1840), aquaculture, biocontrol effect, artificial reproduction, viable juveniles, stocking, restoration of population abundance.","PeriodicalId":30821,"journal":{"name":"Ribogospodars''ka Nauka Ukraini","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135295432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Peculiarities of ligulosis of bream (Abramis brama Linnaeus, 1758) and roach (Rutilus rutilus Linnaeus, 1758) in the Dnipro (Zaporizhzhiа) reservoir 第聂伯罗水库鲷鱼(Abramis brama Linnaeus, 1758)和蟑螂(Rutilus Rutilus Linnaeus, 1758)舌病的特点
Ribogospodars''ka Nauka Ukraini Pub Date : 2023-09-29 DOI: 10.15407/fsu2023.03.119
V. Sydorenko, O. Marenkov
{"title":"Peculiarities of ligulosis of bream (Abramis brama Linnaeus, 1758) and roach (Rutilus rutilus Linnaeus, 1758) in the Dnipro (Zaporizhzhiа) reservoir","authors":"V. Sydorenko, O. Marenkov","doi":"10.15407/fsu2023.03.119","DOIUrl":"https://doi.org/10.15407/fsu2023.03.119","url":null,"abstract":"Purpose. Study of the peculiarities of ligula infestation of roach and bream in the Dnipro (Zaporizhzhia) reservoir and the influence of Ligula intestinalis on the morphological and cytometric parameters of infected fish species. Methodology. The work presents the generalized results of studies conducted in 2020-2021 during planned test catches. Sampling was carried out along the shoreline of the Dnieper (Zaporizhzhia) reservoir in the lower part of the villages of Viiskove and Mykilske-na-Dnipri, Dnipropetrovsk region, in the summer-autumn period. The morphological study was carried out by carefully examining the surface of the scales and skin cover. Special attention was paid on fins, the amount of mucus, its consistency, color change, the presence of tumors, hemorrhages, ulcers, cysts, large ectoparasites, and the condition of eyes. The parasitological study was carried out by a complete anatomical dissection of the fish according to the classical method. Blood was collected at the catch site from the heart of the fish using a needle and a syringe into a dry test tube for the biochemical serum analysis. Blood was also collected for the preparation of smears and their subsequent microscopy. Blood smears were fixed with methyl alcohol and stained according to Romanovsky’s method with Giemsa’s standard solution. Blood smears were examined by microscopy at a 40x magnification. Blood smears of non-infected fish were used for comparison. Thirty fields of view were examined in each smear. Findings. In the course of the study, a decrease in the weight and condition factor of infected roach and bream was observed, and irreversible pathological changes in the internal organs caused by the mechanical pressure and migration of parasites were recorded. In addition, deviations from the normal values were found in the formed elements of blood. About 24.3% of erythrocytes in infected specimens of bream and roach were deformed: the cell membrane was not clear, erythrocytes had a slightly inflated shape. In some erythrocytes, the nucleus was faintly visible. Infected specimens had more juvenile forms of erythrocytes compared to non-infected fish. Originality. For the first time, a comparative morphological, cytometric analysis of blood during ligulosis infestation of infected specimens of bream and roach in the Dnipro (Zaporizhzhia) reservoir is presented. Practical value. The study of biological, clinical and epizootic peculiarities of parasitic helminths is of urgent interest for both science and fish farming. The obtained results can be implemented in the practical activity of fish farming and used in the development of measures to limit and prevent the development of parasitic diseases among the ichthyofauna of the Dnipro (Zaporizhzhia) reservoir. Key words: bream, roach, ligula, erythrocytes, albumins.","PeriodicalId":30821,"journal":{"name":"Ribogospodars''ka Nauka Ukraini","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135294004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Histological structure of gills of Prussian carp of the Samara River of the Dnipropetrovsk region 第聂伯罗彼得罗夫斯克地区萨马拉河普鲁士鲤鱼鳃的组织学结构
Ribogospodars''ka Nauka Ukraini Pub Date : 2023-09-29 DOI: 10.15407/fsu2023.03.102
K. Mashkova, T. Sharamok
{"title":"Histological structure of gills of Prussian carp of the Samara River of the Dnipropetrovsk region","authors":"K. Mashkova, T. Sharamok","doi":"10.15407/fsu2023.03.102","DOIUrl":"https://doi.org/10.15407/fsu2023.03.102","url":null,"abstract":"Purpose. To study the histological structure of the gill apparatus of the Prussian carp (Сarassius gibelio) from the point of view of the presence of pathologies with differentiation according to areas with different levels of anthropogenic pressure of the Samara River in the Dnipropetrovsk region. Methodology. The generalized sutdy results were obtained during 2019–2021. The study was conducted within three sampling sites with different types of human impact. The object of the study were age-6 Prussian carps of both sexes. Histological studies were performed in accordance with generally accepted methods. Gills were obtained by anatomical dissection from freshly caught fish. Photographs of histological specimens were taken using a digital camera “SciencelabT5005.17M” and an microscope “Ulab XY-B2TLED”. Measurements of the samples were performed using the “ScienceLabView7”. Statistical processing of the obtained data was carried out in MS Excel 2010. Findings. The conducted histological studies showed that pathological changes in the structure of the gill apparatus were observed in age-6 Prussian carps in all sampling sites of the Samara River. In particular, such pathologies as curvature of lamellae terminals, accumulation of mucus and hyperplasia of the epithelium of gill filaments, fusion of lamellae were found. Single cases of necrosis and twisting of lamellae threads were recorded. Most often, histopathological changes in the gill apparatus were observed in fish caught near the city Novomoskovsk. Organometric studies showed that there was no significant difference between the length and width of gill arches of Prussian carp from different sampling sites. The length and width of the lamellae of the gill apparatus was the largest in individuals sampled near the city Novomoskovsk, which may indicate a greater need for oxygen for the fish body in the specified area. The length of the respiratory lamellae was the lowest in Prissian carp sampled near the village of Novoselivka. The lowest width of the lamellae were found in fish from the village of Khashcheve. The obtained data give reason to conclude that the conditions of existence for Prussian carp are most favorable near the village of Novoselivka. Originality. For the first time the histological structure and pathology of the gills aparatus of Prussian carp in the conditions of the Samara River in the Dnipropetrovsk region was studied. Practical value. The obtained data on the histological changes of the gill apparatus of fish can be used to identify adaptive reactions, will allow assessing the impact of anthropogenic factors on hydrobionts, and will also provide the opportunity to monitor the morpho-physiological state of a specific population under the impact of pollutants of various origins. Key words: Prussian carp, gill apparatus, morphological and histological indicators.","PeriodicalId":30821,"journal":{"name":"Ribogospodars''ka Nauka Ukraini","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135295428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Efficiency of use of prebiotics based on manane oligosaccharides in carp feeding 甘露寡糖益生元在鲤鱼饲料中的利用效率
Ribogospodars''ka Nauka Ukraini Pub Date : 2021-09-30 DOI: 10.15407/fsu2021.03.060
О. Dobryanska
{"title":"Efficiency of use of prebiotics based on manane oligosaccharides in carp feeding","authors":"О. Dobryanska","doi":"10.15407/fsu2021.03.060","DOIUrl":"https://doi.org/10.15407/fsu2021.03.060","url":null,"abstract":"Purpose. Analysis of productive and economic indicators in the cultivation of age-1+ carp as a result of the use of prebiotics based on mannan oligosaccharides (MOS) in feeding. Methodology. Four ponds were used, two of which were experimental and two control ponds, which were stocked with age-1 scaly carp with an average weight of 76–77 g at a stocking density of 2200 specimens/ha. Fish of Experiment 1 and Control 1 were fed with extruded feed with a protein content of 23%, fish in Experiment 2 and Control 2 were fed with a grain mixture with a protein content of 19%. 0.05% prebiotic based on MOS \"Actigen\" was added to the diet of experimental groups of carp throughout the growing season. Optimal conditions for carp farming were ensured including control over oxygen, hydrochemical and hydrobiological regimes in ponds, which were measured according to the generally accepted methods in fish farming. Fishery and economic indicators of growing age-1+ were determined after harvesting fish in autumn. Findings. Major hydrochemical parameters in all ponds were within the normative values. The average seasonal biomass of forage organisms of zooplankton and zoobenthos during the growing season did not differ in the experimental ponds and amounted to 10.33-16.23 g/m3 and 1.23-2.96 g/m3. A positive effect of the use of prebiotics based on MOS in the feeding of age-1+ carp was established. When the prebiotic was given as a part of the extruded feed, the average weight of fish was higher by 11.5%, and fish productivity by 22.7% relative to the control group, while the cost of feed for cultivation was lower by 20%. When it was added to the grain mixture, these values were higher by 12.9%, 16.3% and 16.7%, respectively. The profit, as a result of the use of the prebiotic based on MOS in the composition of the extruded feed increased by 43.3% compared to Control 1, and by 19.1% compared to Control 2 when it was added to the grain mixture. Originality. For the first time, a comparative analysis of the effectiveness of the use of the prebiotic based on MOS in the composition of extruded feed and grain mixtures was performed taking into account productivity and economic indicators. Practical value. The obtained results indicate the feasibility and effectiveness of the use of prebiotics based on MOS in the feeding of age-1+ carp. Introduction of experimental additives to feeds of different composition helps to obtain additional growth and increase the economic component in the cultivation of carp to marketable condition. Key words: carp, prebiotic, feed, productivity, efficiency, profit hydrochemical and hydrobiological indicators.","PeriodicalId":30821,"journal":{"name":"Ribogospodars''ka Nauka Ukraini","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67113595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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