第聂伯罗水库鲷鱼(Abramis brama Linnaeus, 1758)和蟑螂(Rutilus Rutilus Linnaeus, 1758)舌病的特点

V. Sydorenko, O. Marenkov
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引用次数: 0

摘要

目的。第聂伯罗水库蟑螂和鲷鱼舌虫侵染特点及肠舌虫对侵染鱼类形态和细胞参数影响的研究。方法。这项工作介绍了2020-2021年计划测试捕捞期间进行的研究的总体结果。夏秋季节,在第聂伯罗彼得罗夫斯克地区Viiskove村和Mykilske-na-Dnipri村下游沿第聂伯河水库岸线取样。形态学研究是通过仔细检查鳞片表面和皮肤覆盖物进行的。特别注意的是鳍、粘液的数量、粘稠度、颜色变化、肿瘤、出血、溃疡、囊肿、大的体外寄生虫的存在以及眼睛的状况。寄生虫学研究是根据经典方法对鱼进行完整的解剖。在捕获部位用针和注射器从鱼的心脏采集血液,放入干试管中进行生化血清分析。还采集血液用于涂片的制备和随后的显微镜检查。血液涂片用甲醇固定,用吉姆萨标准溶液按罗曼诺夫斯基法染色。用40倍放大镜检查血液涂片。未感染鱼的血涂片用于比较。每个涂片检查30个视野。发现。在研究过程中,观察到感染的蟑螂和鲷鱼的体重和条件因子下降,并记录了由寄生虫的机械压力和迁移引起的内脏不可逆的病理变化。此外,在血液的形成元素中发现与正常值的偏差。感染的鲷鱼和蟑螂标本中约有24.3%的红细胞变形:细胞膜不清,红细胞略有膨大。在一些红细胞中,细胞核隐约可见。与未感染的鱼相比,感染的鱼有更多的幼红细胞。创意。首次对第聂伯罗水库感染的鲷鱼和蟑螂进行了口舌病侵染期间的血液形态、细胞分析比较。实用价值。寄生蠕虫的生物学、临床和兽疫学特性的研究对科学和养鱼都具有迫切的意义。所得结果可应用于实际养鱼活动,并可用于制定限制和预防第聂伯罗水库鱼系动物寄生虫病发生的措施。关键词:鲤鱼,蟑螂,舌鱼,红细胞,白蛋白。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Peculiarities of ligulosis of bream (Abramis brama Linnaeus, 1758) and roach (Rutilus rutilus Linnaeus, 1758) in the Dnipro (Zaporizhzhiа) reservoir
Purpose. Study of the peculiarities of ligula infestation of roach and bream in the Dnipro (Zaporizhzhia) reservoir and the influence of Ligula intestinalis on the morphological and cytometric parameters of infected fish species. Methodology. The work presents the generalized results of studies conducted in 2020-2021 during planned test catches. Sampling was carried out along the shoreline of the Dnieper (Zaporizhzhia) reservoir in the lower part of the villages of Viiskove and Mykilske-na-Dnipri, Dnipropetrovsk region, in the summer-autumn period. The morphological study was carried out by carefully examining the surface of the scales and skin cover. Special attention was paid on fins, the amount of mucus, its consistency, color change, the presence of tumors, hemorrhages, ulcers, cysts, large ectoparasites, and the condition of eyes. The parasitological study was carried out by a complete anatomical dissection of the fish according to the classical method. Blood was collected at the catch site from the heart of the fish using a needle and a syringe into a dry test tube for the biochemical serum analysis. Blood was also collected for the preparation of smears and their subsequent microscopy. Blood smears were fixed with methyl alcohol and stained according to Romanovsky’s method with Giemsa’s standard solution. Blood smears were examined by microscopy at a 40x magnification. Blood smears of non-infected fish were used for comparison. Thirty fields of view were examined in each smear. Findings. In the course of the study, a decrease in the weight and condition factor of infected roach and bream was observed, and irreversible pathological changes in the internal organs caused by the mechanical pressure and migration of parasites were recorded. In addition, deviations from the normal values were found in the formed elements of blood. About 24.3% of erythrocytes in infected specimens of bream and roach were deformed: the cell membrane was not clear, erythrocytes had a slightly inflated shape. In some erythrocytes, the nucleus was faintly visible. Infected specimens had more juvenile forms of erythrocytes compared to non-infected fish. Originality. For the first time, a comparative morphological, cytometric analysis of blood during ligulosis infestation of infected specimens of bream and roach in the Dnipro (Zaporizhzhia) reservoir is presented. Practical value. The study of biological, clinical and epizootic peculiarities of parasitic helminths is of urgent interest for both science and fish farming. The obtained results can be implemented in the practical activity of fish farming and used in the development of measures to limit and prevent the development of parasitic diseases among the ichthyofauna of the Dnipro (Zaporizhzhia) reservoir. Key words: bream, roach, ligula, erythrocytes, albumins.
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