Biosafety and Biotechnology最新文献_第2页

COMPARISON OF THE RESULTS OF CULTURING CELLS AND VIRUSES ON THE NEW SEPLIFE® LX-MC-DEX1 MICROCARRIER WITH EXISTING MICROCARRIERS 新型seplife®lx-mc-dex1微载体与现有微载体培养细胞和病毒的结果比较

Biosafety and Biotechnology Pub Date : 2023-01-25 DOI: 10.58318/2957-5702-2022-11-13-22

Sh. S. Turyskeldi, Z. Sametova, A. K. Usembay, Y. Bulatov

引用次数: 0

REAL-TIME POLYMERASE CHAIN REACTION USING SYBR GREEN I FLUORESCENT DYE TO DIAGNOSE CANINE DISTEMPER VIRUS sybr绿I荧光染料实时聚合酶链反应诊断犬瘟热病毒

Biosafety and Biotechnology Pub Date : 2022-11-27 DOI: 10.58318/2957-5702-2022-9-6-13

M. K. Isakeev, Zh. Ch. Orozov, M. A. Ahmedjanov, S. A. Dzheenbaeva

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Microbiological control of water quality for the production of biological p 水质微生物控制对生物磷生产的影响

Biosafety and Biotechnology Pub Date : 2022-11-27 DOI: 10.58318/2957-5702-2022-12-56-66

S. Moldagulova, E. Kalimolda, S. Sadikaliyeva, G. Tokkarina, E. Voronina, B. A. Espembetov, А. S. Nurpeisova, М. Kasenov, K. Shorayeva

{"title":"Microbiological control of water quality for the production of biological p","authors":"S. Moldagulova, E. Kalimolda, S. Sadikaliyeva, G. Tokkarina, E. Voronina, B. A. Espembetov, А. S. Nurpeisova, М. Kasenov, K. Shorayeva","doi":"10.58318/2957-5702-2022-12-56-66","DOIUrl":"https://doi.org/10.58318/2957-5702-2022-12-56-66","url":null,"abstract":"one of the main criteria for the biological safety of immunobiological preparations is their sterility. This article presents the results of the evaluation of two methods of direct seeding and membrane filtration. The results of sterility control of the inactivated vaccine against Covid-19 «QazCovid-in®, series № 0400721, № 0410721, № 0420721 are also presented. Evaluation of the sterility of the tested samples of the three vaccine series showed that after incubation, the nutrient medium remained clean both in direct seeding samples and membrane filtration samples, as well as accounting and evaluation of the obtained research data in accordance with the State Pharmacopoeia of the Republic of Kazakhstan. To assess the sensitivity of two methods for determining sterility, samples of immunobiological preparations were experimentally infected with cultures of test strains of St. aureus, C. albicans and C. sporogenes. As a result, the methods of membrane filtration and direct seeding showed the same sensitivity when detecting yeast and anaerobic organisms in all studied concentrations of test strains (10, 1, 0.1 CFU/ml). And for the detection of aerobic microorganisms, the membrane filtration method turned out to be more sensitive compared to the direct seeding method, which is proved by positive results in all samples of test strains with membrane filtration (3/3 in all concentrations) and negative results when setting the direct seeding method (in concentrations of 1 and 0.1 CFU/ml). Thus, the purpose of this study was to evaluate these two methods used to determine the sterility of immunobiological preparations using two methods: direct seeding and membrane filtration","PeriodicalId":307725,"journal":{"name":"Biosafety and Biotechnology","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121555237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

APPROBATION TRIALS OF A VACCINE AGAINST NEWCASTLE DISEASE FROM THE LA SOTA STRAIN 从la sota菌株中获得的抗新城疫疫苗的批准试验

Biosafety and Biotechnology Pub Date : 2022-11-27 DOI: 10.58318/2957-5702-2022-9-34-43

Zhailaubay Kydyrbayev, N. N. Asanjanova, S. Ryskeldinova, E. M. Kozhamkulov, E. K. Kulbekov, E. T. Myrzakhmetov, Zh. A. Tursynova, N. Z. Akmyrzaev

{"title":"APPROBATION TRIALS OF A VACCINE AGAINST NEWCASTLE DISEASE FROM THE LA SOTA STRAIN","authors":"Zhailaubay Kydyrbayev, N. N. Asanjanova, S. Ryskeldinova, E. M. Kozhamkulov, E. K. Kulbekov, E. T. Myrzakhmetov, Zh. A. Tursynova, N. Z. Akmyrzaev","doi":"10.58318/2957-5702-2022-9-34-43","DOIUrl":"https://doi.org/10.58318/2957-5702-2022-9-34-43","url":null,"abstract":"the paper presents the results of an approbation test of the physical and biological properties of an experimental series of a vaccine against Newcastle disease from the La-Sota strain in the conditions of the National Reference Center for Veterinary Medicine of the Committee for Veterinary Control and Supervision of the Ministry of Agriculture of the Republic of Kazakhstan (CVC and SMA RK) and the Research Institute of Biological Problems security (RIBSP). The aim of the study was to test a vaccine against Newcastle disease from the La-Sota strain. When performing the work, general virological and immunological research methods were used. The results of approbation studies showed that the vaccine was free of contaminants and its biological activity was 9,03 ± 0,14 lg EID50/cm3. The vaccine is harmless to chickens of 40 days of age. The vaccine is immunogenic for chickens. The average titers of antibodies to the Newcastle disease virus on the 16th day after vaccination is 1:127,2 in the hemagglutination inhibition test (HITA). The vaccine meets all the required requirements in terms of its physico-biological properties and is suitable for specific prophylaxis of Newcastle disease. Approbation tests of the physical, biological properties of the vaccine were completed with positive results and protocols, research acts were signed by members of the Commission of the CVC and SMA RK. It is recommended to use the vaccine on a large scale in the conditions of poultry farms, private farmsteads for the specific prevention of Newcastle disease.","PeriodicalId":307725,"journal":{"name":"Biosafety and Biotechnology","volume":"82 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114621917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

THE RESULTS OF LABORATORY STUDIES TO DETERMINE THE CAUSE OF THE DEATH OF RABBITS 实验室研究的结果确定了兔子的死亡原因

Biosafety and Biotechnology Pub Date : 2022-11-27 DOI: 10.58318/2957-5702-2022-9-59-67

M. Akhmedzhanov, E. D. Krutskaya, M. K. Isakeev

引用次数: 0

DISTRIBUTION OF TOXOCAROSIS OF DOGS AND CATS IN TAJIKISTAN 塔吉克斯坦狗和猫的弓形虫病分布

Biosafety and Biotechnology Pub Date : 2022-11-27 DOI: 10.58318/2957-5702-2022-9-53-58

Sh. Sh. Razikov, M. U. Assoeva

引用次数: 0

SCALING OF VERO CELL CULTURE FOR THE PRODUCTION OF BIOLOGICAL PRODUCTS 生物制品生产中细胞培养的规模化

Biosafety and Biotechnology Pub Date : 2022-11-27 DOI: 10.58318/2957-5702-2022-9-44-52

B. A. Seidakhmetova, G. A. Zhapparova, L. G. Marakhovskaya, A. A. Terebay, A. Nakhanov

{"title":"SCALING OF VERO CELL CULTURE FOR THE PRODUCTION OF BIOLOGICAL PRODUCTS","authors":"B. A. Seidakhmetova, G. A. Zhapparova, L. G. Marakhovskaya, A. A. Terebay, A. Nakhanov","doi":"10.58318/2957-5702-2022-9-44-52","DOIUrl":"https://doi.org/10.58318/2957-5702-2022-9-44-52","url":null,"abstract":"vaccine production is considered the most effective way to prevent and control the spread of infectious diseases. The transplantable Vero cell line is now widely used for vaccine production.The aim of this study was to investigate the optimal parameters for growing Vero cell cultures in cell factories for large-scale vaccine production. For the scaling process, the seed concentration of cells was determined. The results of the studies showed that at a cell inoculum concentration of 2.0x105 cells/ml, a monolayer is formed in 1-2 days and is the most optimal for cultivating most viruses. Multilayer cell culture systems for industrial scales provide a one-stop production solution. The convenient and cost-effective format of cell factories saves space, time and labor and reduces the risk of contamination.In this paper, Vero cell culture is scaled up in cell factories because this cell culture method is necessary to create cost-effective workflows in large volumes. The study showed that the inoculum concentration, the cell proliferation index, and the ratio of the dispersing mixture for cell removal are comparable to routinely used T-vials.","PeriodicalId":307725,"journal":{"name":"Biosafety and Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125515919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CAMELPOX: BRIEF SCIENTIFIC OVERVIEW 骆驼痘:简要的科学概述

Biosafety and Biotechnology Pub Date : 2022-11-27 DOI: 10.58318/2957-5702-2022-9-6-18

A. Omurtay, M. Tuyskanova, K. D. Zhugunisov

引用次数: 0

CHARACTERISTICS OF BRUCELLA CIRCULATING ON THE TERRITORY OF THE REPUBLIC OF KAZAKHSTAN 在哈萨克斯坦共和国境内流行的布鲁氏菌的特征

Biosafety and Biotechnology Pub Date : 2022-11-27 DOI: 10.58318/2957-5702-2022-9-24-33

Sholpan A. Baramova, A. T. Daugalieva, A. Abutalip, B. Otarbayev, A. Daniyal, R. I. Akhatova

{"title":"CHARACTERISTICS OF BRUCELLA CIRCULATING ON THE TERRITORY OF THE REPUBLIC OF KAZAKHSTAN","authors":"Sholpan A. Baramova, A. T. Daugalieva, A. Abutalip, B. Otarbayev, A. Daniyal, R. I. Akhatova","doi":"10.58318/2957-5702-2022-9-24-33","DOIUrl":"https://doi.org/10.58318/2957-5702-2022-9-24-33","url":null,"abstract":"as you know, one of the economically and socially significant diseases that are widespread in the territory of the Republic of Kazakhstan is brucellosis, which occupies a dominant place in the general infectious pathology of animals. Brucellosis is one of the most dangerous zoonotic infectious diseases for humans. The results of comparative studies on the identification of the causative agent of brucellosis in the Republic of Kazakhstan for several years using the bacteriological method and PCR indicate that the use of the above methods to determine the epizootological status of herds of animals in the primary diagnosis of brucellosis is impractical due to the low degree of informativeness of these tests. PCR is recommended for identification and genotyping of isolated brucella cultures from pathological material. As a result of diagnostic studies of biomaterial obtained from animals from economically disadvantaged brucellosis subjects of Kazakhstan and border countries (Russia and Kyrgyzstan), the authors identified brucella cultures, which were subsequently subjected to the study of their biological and molecular genetic properties. For the identification and genotyping of isolated brucella cultures, researchers suggest using MLVA as the most effective method for reliably determining their genotypic characteristics.","PeriodicalId":307725,"journal":{"name":"Biosafety and Biotechnology","volume":"63 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126648027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sequencing and phylogenetic analysis of the ORF3a gene variant B.1.1.7 SARS-CoV-2 virus SARS-CoV-2病毒ORF3a基因变异B.1.1.7的测序和系统发育分析

Biosafety and Biotechnology Pub Date : 2022-06-27 DOI: 10.58318/2957-5702-2022-9-44-54

B. Usserbayev, N. S. Кozhabergenov, A. Melisbek, M. Shirinbekov, A. I. Tagayev, Y. Burashev

{"title":"Sequencing and phylogenetic analysis of the ORF3a gene variant B.1.1.7 SARS-CoV-2 virus","authors":"B. Usserbayev, N. S. Кozhabergenov, A. Melisbek, M. Shirinbekov, A. I. Tagayev, Y. Burashev","doi":"10.58318/2957-5702-2022-9-44-54","DOIUrl":"https://doi.org/10.58318/2957-5702-2022-9-44-54","url":null,"abstract":"The problem of \"new\" infectious diseases has now acquired particular relevance for national and international health systems. One of these infections is the SARS-CoV-2 coronavirus. Due to the rapid spread of a new type of coronavirus in the world, molecular genetic studies of this virus are of great importance. This will help to understand the nature of the virus and develop antiviral drugs to prevent the disease. The aim is to identify a missense mutation in the ORF3 gene of the studied coronavirus strain SARS-CoV-2/human/KAZ/Britain. This paper presents the results of the development and sequencing of the ORF3 gene of the coronavirus strain SARS-Cov-2/human/KAZ/Britain. For the development and sequencing by the Sanger method of the ORF3 gene, 4 pairs of primers were developed, with an overlap of 100-150 bp. The changes in the ORF3 gene compared to other strains whose data were obtained from the GISAID database were analyzed. As a result, the obtained amino acid sequences of the ORF3a gene of variant B.1.1.7 were compared with the reference strain HCoV-19/Wuhan/Hu-1/2019, where one mutation was determined at position 149: W (tryptophan) → L (leucine). The phylogenetic affiliation of the studied virus strain was also determined, where the genetic distance between the studied strains of the SARS-CoV-2 virus was determined. Thus, the greatest similarity with the studied strain were: HCoV-19/Wuhan/Hu-1/2019, HCoV-19/England/MILK-9E05B3/2020, HCoV-19/Japan/IC-0446/2020, HCoV-19/Germany/NI-IOV-MHH15/2020, and the strain HCoV-19/Argentina/PAIS-F0418/2021 significantly distanced itself from the SARS-CoV-2/human/KAZ/Britain strain.","PeriodicalId":307725,"journal":{"name":"Biosafety and Biotechnology","volume":"409 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114938557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0