Biosafety and Biotechnology最新文献

STUDY OF THE EPIZOOTIC SITUATION OF HORSE WASHING IN THE ALMATY REGION 阿拉木图地区洗马流行情况调查

Biosafety and Biotechnology Pub Date : 2023-01-26 DOI: 10.58318/2957-5702-2022-12-44-55

B. Yespembetov, M. Sarmykova, A. Sambetbaev, E. B. Serikbay, N. Syrym, N. Zinina, A. M. Anarbekova, K. K. Tileukhanov

{"title":"STUDY OF THE EPIZOOTIC SITUATION OF HORSE WASHING IN THE ALMATY REGION","authors":"B. Yespembetov, M. Sarmykova, A. Sambetbaev, E. B. Serikbay, N. Syrym, N. Zinina, A. M. Anarbekova, K. K. Tileukhanov","doi":"10.58318/2957-5702-2022-12-44-55","DOIUrl":"https://doi.org/10.58318/2957-5702-2022-12-44-55","url":null,"abstract":"This article presents the results of statistical data as of July 1, compared with the same date last year, the number of horses as a whole increased by 8.5%, amounting to 3372.5 thousand heads as of July 1. However, during the winter months (January and February), the number of horses in Kazakhstan decreased by 1.3%. In absolute terms, the number of horses in January decreased by 20.2 thousand heads, and in February by another 16.6 thousand heads. In recent years, the country has seen an increase in the incidence of mytomy not only among foals, but also among adult livestock. In order to conduct epizootological monitoring of washing of horses, according to the calendar plan of the project IRN 08855635, from 2020-2022 scientific expeditions were organized on the territory of the Almaty region of the republic with the sampling of pathological material from sick animals. As a result of the purposeful organization and conduct of expedition trips, the necessary epidemiological data were collected and the analysis of ongoing veterinary and sanitary measures was carried out. As a result of this stage, samples were collected in a total of 112 samples of biomaterial from sick washed foals during sowing, it was possible to isolate the culture of the microbe only in 3 cases. This circumstance is due to the fact that the modified forms of mytaceous streptococcus causing atypical cases of the disease are very difficult to cultivate. According to the results of the study, bacterial isolates were identified as the species Streptococcus equi.","PeriodicalId":307725,"journal":{"name":"Biosafety and Biotechnology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129158504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ANTHRAX AND THE RISKS OF THE DISEASE IN THE REPUBLIC OF KAZAKHSTAN 炭疽及其在哈萨克斯坦共和国的风险

Biosafety and Biotechnology Pub Date : 2023-01-26 DOI: 10.58318/2957-5702-2022-11-23-31

T. S. Chukayeva

引用次数: 0

ROLE OF CORONAVIRUS IN ACUTE RESPIRATORY VIRAL INFECTIONS 冠状病毒在急性呼吸道病毒感染中的作用

Biosafety and Biotechnology Pub Date : 2023-01-26 DOI: 10.58318/2957-5702-2022-11-46-50

R. Nurgaziev, E. D. Krutskaya

引用次数: 0

SELECTION OF SPECIFIC PRIMERS FOR PCR IN BOVINE LEUKEMIA VIRUS 牛白血病病毒PCR特异性引物的选择

Biosafety and Biotechnology Pub Date : 2023-01-26 DOI: 10.58318/2957-5702-2022-12-24-35

U. Z. Kuzhebayeva, I. Beishova, V. Ulyanov, Т. V. Ulyanova, Alexandr Kovalchuk, N. Ginayatov, А. Z. Sidarova

{"title":"SELECTION OF SPECIFIC PRIMERS FOR PCR IN BOVINE LEUKEMIA VIRUS","authors":"U. Z. Kuzhebayeva, I. Beishova, V. Ulyanov, Т. V. Ulyanova, Alexandr Kovalchuk, N. Ginayatov, А. Z. Sidarova","doi":"10.58318/2957-5702-2022-12-24-35","DOIUrl":"https://doi.org/10.58318/2957-5702-2022-12-24-35","url":null,"abstract":"the polymerase chain reaction (PCR) method is widely used to solve various problems. PCR is widely used for the detection of bacterial and viral pathogens. Primers are a very important component of PCR, since the specificity of amplification depends primarily on them. They are necessary for the enzyme to work and are specific to the fragment of interest. Based on the results of the selection of nucleotide sequences of the genome or individual fragments of the virus RNA from the international database on the NCBI website, a large number of sequences for the bovine leukemia virus were identified, which are stored in gene banks and updated daily with new data. The construction of primers in compliance with the necessary parameters is carried out using various computer programs, the main of which are MUSCLE, UGENE V.36.0, Primer-BLAST, Oligo Analyzer and others. The designed primers were then synthesized on the Expedite 8909 oligonucleotide synthesizer, according to the instructions attached to the device. As a result of our experiments, we selected and synthesized specific synthetic oligonucleotides env (g51)_1 and env (g51)_2, for setting up PCR for bovine leukemia.","PeriodicalId":307725,"journal":{"name":"Biosafety and Biotechnology","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128574234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CULTURAL PROPERTIES OF THE SOUTH AFRICAN VARIANT OF OMICRON VIRUS SARS-CoV-2 OF CORONAVIRUS INFECTION COVID-19 冠状病毒感染COVID-19的南非组粒病毒变体SARS-CoV-2的文化特性

Biosafety and Biotechnology Pub Date : 2023-01-26 DOI: 10.58318/2957-5702-2022-12-36-43

B. Myrzakhmetova, K. B. Bisenbayeva, M. S. Kaukarbayeva, Y. Burashev, L. Kutumbetov

引用次数: 0

SELECTION OF HIGHLY PRODUCTIVE GENOTYPES OF SUGAR SORGHUM (SORGHUM BICOLOR L) FOR FURTHER USE IN PHYTOREMEDIATION OF SOILS FROM HEAVY METALS 糖高粱高产基因型的选择及其在土壤重金属修复中的进一步应用

Biosafety and Biotechnology Pub Date : 2023-01-26 DOI: 10.58318/2957-5702-2022-11-32-38

A. Sagimbayeva

{"title":"SELECTION OF HIGHLY PRODUCTIVE GENOTYPES OF SUGAR SORGHUM (SORGHUM BICOLOR L) FOR FURTHER USE IN PHYTOREMEDIATION OF SOILS FROM HEAVY METALS","authors":"A. Sagimbayeva","doi":"10.58318/2957-5702-2022-11-32-38","DOIUrl":"https://doi.org/10.58318/2957-5702-2022-11-32-38","url":null,"abstract":"at the moment, soil pollution is the main environmental problem, which leads to a great need for the restoration of contaminated soils using the most appropriate and effective cleaning methods. Conventional remediation and bioremediation of contaminated sites usually involves the physical removal of pollutants or biological exposure with the help of microorganisms. Basic physical recovery strategies are expensive, non-specific, and often render the soil unsuitable for further use by disrupting the microenvironment. Due to these concerns, there has been increased interest in environmentally friendly and sustainable approaches, such as phytostabilization, phytoremediation and phytofiltration for cleaning contaminated sites. In this article, special attention is paid to the selection of highly productive genotypes of sugar sorghum for further use in phytoremediation of soils from heavy metals. Removal of heavy metals from the environment with the help of plants in the modern world is a very effective and highly profitable method. In this regard, the identification and study of promising plants is the basis of successful biotechnology. From this point of view, sorghum crops are attractive. They have properties such as resistance to extreme drought, heat and salt resistance","PeriodicalId":307725,"journal":{"name":"Biosafety and Biotechnology","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128789742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

APPROVAL OF THE TEST-SYSTEM FOR THE INDICATION AND IDENTIFICATION OF ASPERGILLUS FLAVUS BY THE METHOD OF POLYMERASE CHAIN REACTION IN THE “REAL TIME” MODE 批准采用聚合酶链反应法“实时”模式对黄曲霉进行指示和鉴定的测试系统

Biosafety and Biotechnology Pub Date : 2023-01-26 DOI: 10.58318/2957-5702-2022-12-67-73

N. Feoktistova, E. Suldina, A. Mastilenko, A. Lomakin

{"title":"APPROVAL OF THE TEST-SYSTEM FOR THE INDICATION AND IDENTIFICATION OF ASPERGILLUS FLAVUS BY THE METHOD OF POLYMERASE CHAIN REACTION IN THE “REAL TIME” MODE","authors":"N. Feoktistova, E. Suldina, A. Mastilenko, A. Lomakin","doi":"10.58318/2957-5702-2022-12-67-73","DOIUrl":"https://doi.org/10.58318/2957-5702-2022-12-67-73","url":null,"abstract":"the article presents the results of studies on approbation of a test system for the indication and identification of microscopic fungi Aspergillus flavus by the polymerase chain reaction method with real-time detection. Using software Multiple Sequence Alignment Viewer 1.22.1 and UGENA 44.0. The test system for A. flavus includes specific primers: forward primer (f) 5’-3’ GGGCCCGCAGCAAGAATAC, reverse primer (r) 3’-5’ ACGAGTTGTCACCTTCCCGAGA; fluorescent dye: HEX, probe - CGGTTCGCTTTGGTCATCGT, quencher BHQ2. Reaction protocol: preliminary denaturation - 95 0C - 5 minutes (1 cycle); denaturation - 95 0C - 5 sec, annealing - 60 0C - 15 sec (50 cycles). Probe: AGCATAGGCTGATGCTCGTAGGC, fluorescent dye - ROX, quencher - BHQ-2. The sensitivity of the test system is 1000 cells. The optimal concentration of primers was set equal to 9 pM of each primer per reaction. The optimal probe concentration is 0.4 pM. The results obtained indicate that the use of dichotomous keys does not allow the most accurate differentiation of phytopathogenic fungi A. flavus. A new approach to the identification of isolates confirmed the belonging of 15 isolated strains to the species A. flavus out of 20 isolated from corn samples with signs that manifest themselves as root rot and wilting, and initially typed as Aspergillus based on the study of cultural and morphological properties. The study was carried out according to the thematic plan-task of the Ministry of agriculture of the Russian Federation, the registration number of the INIS RTD 122030200367-8.","PeriodicalId":307725,"journal":{"name":"Biosafety and Biotechnology","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123363669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SUBTROPIC PERSIMMON FRUIT – A SOURCE OF ANTIOXIDANTS 亚热带柿子——抗氧化剂的来源

Biosafety and Biotechnology Pub Date : 2023-01-26 DOI: 10.58318/2957-5702-2022-12-6-23

Ye. Fokina

引用次数: 0

BIOLOGICAL PROPERTIES OF CL. PERFRINGENS STRAINS 氯离子的生物学特性。PERFRINGENS菌株

Biosafety and Biotechnology Pub Date : 2023-01-26 DOI: 10.58318/2957-5702-2022-11-39-45

Т. A. Toleukhan, Z. Kirkimbayeva, А. Z. Zhylkaidar

引用次数: 0

GEOGRAPHICAL DISTRIBUTION OF ANTHRAX FOCI IN THE CENTRAL-EASTERN REGIONS OF TAJIKISTAN 塔吉克斯坦中东部地区炭疽疫源地的地理分布

Biosafety and Biotechnology Pub Date : 2023-01-25 DOI: 10.58318/2957-5702-2022-11-6-12

A. Muminov, Shuhrat N. Jumaev, M. Asrorzoda

引用次数: 0