B. Myrzakhmetova, K. B. Bisenbayeva, M. S. Kaukarbayeva, Y. Burashev, L. Kutumbetov
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引用次数: 0
摘要
本文介绍了冠状病毒感染COVID-19的SARS-CoV-2病毒遗传变异组粒的培养特性研究数据。最允许这种病毒变异繁殖的生物系统是Vero、MA-104、MARC-145、PK-15和SPEV细胞培养。在所研究的细胞系中培养SARS-CoV-2病毒遗传变异Omicron的生产条件为:细胞播种浓度20万个/cm3，感染倍数- 0.01 TCD50/细胞，病毒培养周期-2天，收集病毒前细胞病变面积单层- 75-80%，病毒生物活性范围为4.14±0.31 ~ 6.66±0.22 lg TCD50/ cm3。
CULTURAL PROPERTIES OF THE SOUTH AFRICAN VARIANT OF OMICRON VIRUS SARS-CoV-2 OF CORONAVIRUS INFECTION COVID-19
this paper presents data on the study of the cultural properties of the genetic variant Omicron of the SARS-CoV-2 virus of the coronavirus infection COVID-19. The most permissive biological systems for the reproduction of this virus variant are Vero, MA-104, MARC-145, PK-15, and SPEV cell cultures. The productive conditions for cultivating the genetic variant Omicron of the SARS-CoV-2 virus in the studied cell lines turned out to be the following parameters: seeding concentration of cells 200 thousand/cm3, multiplicity of infection – 0.01 TCD50/cell, virus cultivation period – 2 days, cell lesion area monolayer before collecting the virus – 75-80%, the biological activity of the virus in the range from 4.14±0.31 to 6.66±0.22 lg TCD50/cm 3.
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