在哈萨克斯坦共和国境内流行的布鲁氏菌的特征

Sholpan A. Baramova, A. T. Daugalieva, A. Abutalip, B. Otarbayev, A. Daniyal, R. I. Akhatova
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摘要

如你们所知,在哈萨克斯坦共和国境内广泛传播的具有重大经济和社会意义的疾病之一是布鲁氏菌病,它在动物的一般传染性病理中占主导地位。布鲁氏菌病是人类最危险的人畜共患传染病之一。哈萨克斯坦共和国数年来利用细菌学方法和聚合酶链反应(PCR)对布鲁氏菌病病原鉴定进行的比较研究结果表明,在布鲁氏菌病的初步诊断中,使用上述方法来确定动物群的流行病学状况是不切实际的,因为这些检测的信息程度较低。PCR被推荐用于从病理材料中分离的布鲁氏菌培养物的鉴定和基因分型。由于对从哈萨克斯坦和边境国家(俄罗斯和吉尔吉斯斯坦)经济条件不利的布鲁氏菌病患者身上获得的动物生物材料进行诊断研究,作者确定了布鲁氏菌培养物,随后对其生物学和分子遗传特性进行了研究。对于分离的布鲁氏菌培养物的鉴定和基因分型,研究人员建议使用MLVA作为可靠地确定其基因型特征的最有效方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
CHARACTERISTICS OF BRUCELLA CIRCULATING ON THE TERRITORY OF THE REPUBLIC OF KAZAKHSTAN
as you know, one of the economically and socially significant diseases that are widespread in the territory of the Republic of Kazakhstan is brucellosis, which occupies a dominant place in the general infectious pathology of animals. Brucellosis is one of the most dangerous zoonotic infectious diseases for humans. The results of comparative studies on the identification of the causative agent of brucellosis in the Republic of Kazakhstan for several years using the bacteriological method and PCR indicate that the use of the above methods to determine the epizootological status of herds of animals in the primary diagnosis of brucellosis is impractical due to the low degree of informativeness of these tests. PCR is recommended for identification and genotyping of isolated brucella cultures from pathological material.         As a result of diagnostic studies of biomaterial obtained from animals from economically disadvantaged brucellosis subjects of Kazakhstan and border countries (Russia and Kyrgyzstan), the authors identified brucella cultures, which were subsequently subjected to the study of their biological and molecular genetic properties. For the identification and genotyping of isolated brucella cultures, researchers suggest using MLVA as the most effective method for reliably determining their genotypic characteristics.
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