Molecular Horticulture最新文献_第6页

Dissecting G-protein signaling pathways in the fruit pathogen Penicillium expansum: implications for pathogenesis and patulin production. 解剖果实病原菌扩张青霉的g蛋白信号通路：对发病机制和展霉素产生的影响。

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Molecular Horticulture Pub Date : 2026-04-08 DOI: 10.1186/s43897-025-00211-w

Yong Chen, Dongying Xu, Mengyang Xing, Tong Chen, Boqiang Li, Shiping Tian

{"title":"Dissecting G-protein signaling pathways in the fruit pathogen Penicillium expansum: implications for pathogenesis and patulin production.","authors":"Yong Chen, Dongying Xu, Mengyang Xing, Tong Chen, Boqiang Li, Shiping Tian","doi":"10.1186/s43897-025-00211-w","DOIUrl":"10.1186/s43897-025-00211-w","url":null,"abstract":"Penicillium expansum causes blue mold in various fruits and produces patulin, a mycotoxin of significant economic and food safety concern. Fungal infection relies on sensing and adapting to environmental cues via G-protein signaling pathways. Despite its importance, the understanding of how these pathways contribute to the infection process and patulin biosynthesis of P. expansum remains limited. Here, we characterized five G protein subunits (PeGαⅠ, PeGαⅡ, PeGαⅢ, PeGβ, and PeGγ), all highly expressed during hyphal growth and fruit infection. Deletion of these G protein genes leads to various defects in hyphal growth, virulence, and patulin production. Notably, ΔPeGαⅠ, ΔPeGβ, and ΔPeGγ mutants showed severe defects, with minimal disease symptoms in early infection stages and at least a 50% decrease in patulin production. These mutants displayed straight hyphal growth, reduced branching, and significantly decreased vitality. RNA sequencing analysis revealed that this abnormal growth is governed by pathways associated with fungal hyphal polarity. Furthermore, PeGαⅠ and PeGαⅢ are crucial for cAMP-PKA signaling, while PeGβ and PeGγ modulate MAPK signaling, both pathways influencing virulence and patulin production. Together, these findings provide evidence that G proteins regulate virulence and patulin production through the regulation of downstream pathways and their broader effects on fungal hyphal polarity.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13059215/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147634490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Haplotype-resolved genome assembly provides insights into the unique floral scent of Rosa rugosa originated in China. 单倍型解析基因组组装提供了对原产于中国的玫瑰独特花香的见解。

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Molecular Horticulture Pub Date : 2026-04-07 DOI: 10.1186/s43897-025-00210-x

Xi Cheng, Xin Geng, Dan Gao, Hongli Wang, Guoliang Wang, Dongliang Chen, Kang Gao, Tianyi Wang, Chengzhi Jiao, Beibei Jiang, Conglin Huang, Fei Shen

{"title":"Haplotype-resolved genome assembly provides insights into the unique floral scent of Rosa rugosa originated in China.","authors":"Xi Cheng, Xin Geng, Dan Gao, Hongli Wang, Guoliang Wang, Dongliang Chen, Kang Gao, Tianyi Wang, Chengzhi Jiao, Beibei Jiang, Conglin Huang, Fei Shen","doi":"10.1186/s43897-025-00210-x","DOIUrl":"10.1186/s43897-025-00210-x","url":null,"abstract":"Rosa rugosa is an important aromatic plant and produces flowers that are used in medicine, food, plant essential oils, hydrosols, and other aromatic products. 2-phenylethanol (2-PE) is the main effective substance in the volatile organic compounds of rose fragrance, and its synthesis mechanism in R. rugosa needs to be investigated. We herein present a haplotype-resolved genome of R. rugosa cv. Hanxiang (HX). Pathways mediating the synthesis of scent-related metabolites were deciphered in depth. Allelic imbalances reveal the distinct roles of different haplotyping in shaping the key trait including fragrance during the flower development stages. We deciphered the population structure and genetic composition of rose. Selected and mutated genes in groups of separated aromas including but not limited to one rate-limiting enzymes, primary amine oxidases, is responsible for the high level of 2-PE biosynthesis in R. rugosa, which could provide new genetic resources for enhancing aroma in other species.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13055005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147628790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MaHSFA2c modulates high temperature-inhibited chlorophyll breakdown in banana fruit by directly inducing the transcription of the E3 ligase gene MaBAH1. MaHSFA2c通过直接诱导E3连接酶基因MaBAH1的转录调节香蕉果实中高温抑制的叶绿素分解。

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Molecular Horticulture Pub Date : 2026-04-03 DOI: 10.1186/s43897-025-00209-4

Wei Wei, Meng-Xue Liu, Jia Si, Qi Luo, Yumei Zhang, Jian-Fei Kuang, Jian-Ye Chen, Wang-Jin Lu, Wei Shan

{"title":"MaHSFA2c modulates high temperature-inhibited chlorophyll breakdown in banana fruit by directly inducing the transcription of the E3 ligase gene MaBAH1.","authors":"Wei Wei, Meng-Xue Liu, Jia Si, Qi Luo, Yumei Zhang, Jian-Fei Kuang, Jian-Ye Chen, Wang-Jin Lu, Wei Shan","doi":"10.1186/s43897-025-00209-4","DOIUrl":"10.1186/s43897-025-00209-4","url":null,"abstract":"Bananas (Musa acuminata, AAA group) ripening at temperatures higher than 24 °C prevent yellow skin development, resulting in green ripening, and significantly affecting the commercial potential of the fruit. Our recent study discovered that high temperature (HT) triggers the E3 ubiquitin ligase MaBAH1 to degrade MaMYB60, thereby suppressing MaMYB60's transactivation of chlorophyll catabolic genes. However, the mechanism by which HT stimulates MaBAH1 expression remains unknown. In this study, a heat shock transcription factor, termed MaHSFA2c, was identified as an upstream regulator of MaBAH1. MaHSFA2c gene expression and protein levels were up-regulated in green-ripened fruit at 30 °C compared to yellow-ripened fruit at 20 °C. MaHSFA2c directly activated the transcription of MaBAH1. Importantly, silencing MaHSFA2c in banana fruit suppressed MaBAH1 expression, reducing MaBAH1-mediated MaMYB60 degradation. This promoted MaMYB60's activation of chlorophyll degradation, thereby weakening green-ripening at HT. In the context of banana fruit, we have demonstrated the existence of a dynamic regulatory module consisting of MaHSFA2c and MaBAH1 that manages chlorophyll catabolism inhibition under HT. These findings enhance our understanding of the transcriptional regulation involved in the loss of fruit quality due to temperature stress and are expected to aid in developing strategies to manage banana green-ripening.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13047763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147610213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fine-mapping reveals BcNAC153 act as a negative regulator controlling high temperature tolerance in Brassica rapa. 精细图谱显示，BcNAC153在油菜耐高温性中起负调控作用。

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Molecular Horticulture Pub Date : 2026-04-02 DOI: 10.1186/s43897-025-00208-5

Gaofeng Liu, Entong Li, Qian Hu, Yaliang Xu, Changwei Zhang, Ying Li, Zixin Zhang, Xilin Hou

{"title":"Fine-mapping reveals BcNAC153 act as a negative regulator controlling high temperature tolerance in Brassica rapa.","authors":"Gaofeng Liu, Entong Li, Qian Hu, Yaliang Xu, Changwei Zhang, Ying Li, Zixin Zhang, Xilin Hou","doi":"10.1186/s43897-025-00208-5","DOIUrl":"10.1186/s43897-025-00208-5","url":null,"abstract":"High temperature stress severely reduces yield and quality in cool-climate adapted NHCC vegetable crops. It is crucial to identify high temperature response regulating genes and analyze the molecular mechanisms of NHCC in order to develop heat-resistant varieties. Here, a whole genome resequencing was conducted on NHCC001, NHCC002, and 312 F2 populations for QTLs mapping. A high-density bin-map containing 3608 bin markers was constructed covering 1156.88 cM, with an average adjacent interval of 0.32 cM. A major QTL for high temperature stress tolerance was identified in A01 chromosome, explaining 6.101% of phenotype variation. And BcNAC153, a NAC transcription factor, was captured and validated as a critical negative regulator for high temperature tolerance. The promoter activity of BcNAC153 was found to be regulated by high temperature, and BcMYB44 can inhibit the transcription of BcNAC153 by binding to the promoter of BcNAC153. In addition, this study reveals that BcCRK1 facilitates the ubiquitin-mediated degradation of BcNAC153, which affects the expression of downstream genes related to chlorophyll degradation, PCD, and ROS accumulation. Overall, this study sheds light on the molecular mechanisms underlying the response of NHCC to high temperature stress, and provides valuable insights for developing heat-resistant vegetable varieties.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13045127/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147595313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a low-penetrance mitosis instead of meiosis system in tomato. 番茄低外显率有丝分裂替代减数分裂系统的建立。

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Molecular Horticulture Pub Date : 2026-04-01 DOI: 10.1186/s43897-025-00207-6

Tong Zhao, Yi Zhang, Jiayao Wu, Iqbal Hussain, Weiqiang Li, Fan Ping, Kaiwen Liu, Changtian Pan, Xiaolin Yu

引用次数: 0

The VaATG6-VaBI-1 module coordinates ER-associated autophagy and ROS homeostasis for cold tolerance in grapevine. vatg6 - vabi -1模块在葡萄抗寒性中协调er相关的自噬和ROS稳态。

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Molecular Horticulture Pub Date : 2026-03-10 DOI: 10.1186/s43897-025-00206-7

Qiaoling Zheng, Fang Ma, Chang Liu, Qinhan Yu, Ningbo Zhang, Weirong Xu

{"title":"The VaATG6-VaBI-1 module coordinates ER-associated autophagy and ROS homeostasis for cold tolerance in grapevine.","authors":"Qiaoling Zheng, Fang Ma, Chang Liu, Qinhan Yu, Ningbo Zhang, Weirong Xu","doi":"10.1186/s43897-025-00206-7","DOIUrl":"10.1186/s43897-025-00206-7","url":null,"abstract":"Cold stress is a major constraint on plant productivity, especially in perennial crops like grapevine (Vitis spp.), where it disrupts cellular homeostasis and induces oxidative damage. Here, we identify VaATG6, a key regulator of autophagy, and its interaction with VaBI-1, an ER-localized modulator, as central components of the cold stress response in Vitis amurensis. Cold exposure rapidly induced VaATG6 transcription and triggered its dynamic relocalization from the nucleus to the ER and autophagosomes, enhancing autophagic flux, reducing reactive oxygen species (ROS) accumulation, and improving cold tolerance. In contrast, VaBI-1 acted as a modulator, fine-tuned autophagy and ROS levels through its interaction with VaATG6. Notably, cold stress strengthened the VaATG6-VaBI-1 interaction, linking a coordinated mechanism that integrated autophagy with ER homeostasis. Overexpression of VaATG6 in cold-sensitive V. vinifera enhanced antioxidant defenses and promoted soluble sugar accumulation, significantly improving cold tolerance. Conversely, VaATG6 loss-of-function mutants exhibited impaired autophagy, elevated ROS accumulation, and increased cold sensitivity. These findings establish the VaATG6-VaBI-1 regulatory module as a core mechanism for cold-induced autophagy and oxidative stress adaptation. Our results highlight VaATG6 overexpression and VaBI-1 modulation as promising genetic strategies to enhance cold tolerance in perennial crops.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12973883/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147391390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic insights into selective signals and local adaptation of a litchi subspecies. 对荔枝亚种的选择信号和局部适应的基因组见解。

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Molecular Horticulture Pub Date : 2026-03-09 DOI: 10.1186/s43897-025-00205-8

Pengfei Wang, Xueren Cao, Hui Zhang, Huanling Li, Huiyun Zhang, Songgang Li, Jiwang Hong, Jian Zheng, Xinping Luo, Chengjie Chen, Lei Zhang, Jiabao Wang

引用次数: 0

Golden leaf formation of Populus nigra is associated with the up-regulation of Stay-Green expression. 黑杨金叶形成与Stay-Green基因表达上调有关。

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Molecular Horticulture Pub Date : 2026-03-06 DOI: 10.1186/s43897-025-00204-9

Wanting Fu, Zimeng Li, Xiaoou Zhai, Haizhen Zhang, Shuang Feng, Aimin Zhou

引用次数: 0

Seizing the key nodes in glabridin biosynthesis network: identification of enzymes for producing licorice hairy roots with high levels of glabridin. 光甘草定生物合成网络关键节点的把握：甘草毛状根高产光甘草定酶的鉴定。

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Molecular Horticulture Pub Date : 2026-03-05 DOI: 10.1186/s43897-025-00202-x

Ningxin Jiang, Xiaoling Ma, Yangxu Wu, Xiaoyi Wei, Yuping Li, Xinyan Guo, Hongxia Wang, Wei Sun, Ling Yuan, Ying Wang, Yongqing Li

{"title":"Seizing the key nodes in glabridin biosynthesis network: identification of enzymes for producing licorice hairy roots with high levels of glabridin.","authors":"Ningxin Jiang, Xiaoling Ma, Yangxu Wu, Xiaoyi Wei, Yuping Li, Xinyan Guo, Hongxia Wang, Wei Sun, Ling Yuan, Ying Wang, Yongqing Li","doi":"10.1186/s43897-025-00202-x","DOIUrl":"10.1186/s43897-025-00202-x","url":null,"abstract":"Glabridin holds significant value in the pharmaceutical and cosmetics industry. Due to the challenges associated with chemical synthesis, Glycyrrhiza glabra (licorice) remains the only source of glabridin. However, its naturally low content is insufficient to meet the increasing market demand. In this study, several inducers of glabridin accumulation-alkali stress, salt-alkali stress, hydroxylamine, 5-azacytidine, and methyl jasmonate (MeJA)-were identified. Using integrated multi-omics analyses and in vitro enzyme assays, two interconnected biosynthetic routes were elucidated: an OMT/ODMT-dependent pathway involving methylation-demethylation cycles and an OMT/ODMT-independent route. Five enzymes, GgIFR (isoflavone reductase), GgTHIS1/2 (7,2',4'-trihydroxyisoflavanol synthases), GgPTS (pterocarpan synthase), GgPTR1/4 (pterocarpan reductases), and GgODMT (O-demethyltransferase), were functionally validated. Using an optimized Agrobacterium rhizogenes-mediated transformation system, we generated transgenic hairy root lines overexpressing these enzymes were generated. GgIFR-overexpressing lines achieved a 44-fold increase in glabridin content (0.507 mg/g DW), comparable to levels in 4-year-old wild roots. This study not only elucidates the complex biosynthetic network of glabridin biosynthesis but also establishes a scalable and sustainable hairy root platform for its industrial production via synthetic biology.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12961851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147356653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The mechanism of dynamic equilibrium of ascorbate redox status mediated by PbrDHAR5 during scald development in pear fruit. PbrDHAR5介导梨果实烫伤发育过程中抗坏血酸氧化还原状态动态平衡的机制

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Molecular Horticulture Pub Date : 2026-03-04 DOI: 10.1186/s43897-025-00203-w

Xu Zhang, Junpeng Niu, Yanmin Du, Lin Guo, Lichao Chen, Min Ma, Xin Qiao, Weiqi Luo, Chunlu Qian, Guodong Wang, Wenhui Wang, Zhen Zhang, Xinli Geng, Qiuqin Zhang, Lanqing Li, Libin Wang, Shaoling Zhang

{"title":"The mechanism of dynamic equilibrium of ascorbate redox status mediated by PbrDHAR5 during scald development in pear fruit.","authors":"Xu Zhang, Junpeng Niu, Yanmin Du, Lin Guo, Lichao Chen, Min Ma, Xin Qiao, Weiqi Luo, Chunlu Qian, Guodong Wang, Wenhui Wang, Zhen Zhang, Xinli Geng, Qiuqin Zhang, Lanqing Li, Libin Wang, Shaoling Zhang","doi":"10.1186/s43897-025-00203-w","DOIUrl":"10.1186/s43897-025-00203-w","url":null,"abstract":"Ascorbate (AsA) redox status participated in the scald development of Pyrus bretschneideri Rehd. fruit as a cellular redox sensor. By a conjoint analysis of metabolites, enzyme activities and gene expression profiles in AsA-GSH cycle of the chilled pear, PbrDHAR5 was characterized as the candidate gene involved in this process. PbrDHAR5, located in cytosol and nucleus, catalyzed DHA reduction into AsA in vitro and in vivo, elevating AsA redox status and thus fruit chilling tolerance; moreover, the catalytic Cys20 residue in PbrDHAR5 played critical role in this reaction. After analyzing the expression profiles of the differentially expressed TFs, PbrWRKY83 demonstrated higher correlation with PbrDHAR5 than others. PbrWRKY83, located in nucleus, could interact with the only two W-box elements in PbrDHAR5 promoter as monomer and then activate its expression, leading to the improvement of AsA redox status and thus fruit chilling tolerance. In a further study, we explored that the H2O2-mediated S-sulfenylation of Cys20 residue in PbrDHAR5 accumulated upon scald development, suppressed its activity, and thus caused the decrement of AsA redox status. Taken together, our results implied that the H2O2-mediated S-sulfenylation of PbrDHAR5 attenuates the role of PbrWRKY83-PbrDHAR5 module, which positively regulates AsA redox status during scald development in pear.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12958699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147349117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0