Molecular Horticulture最新文献_第5页

ClBCM: an EMS mutated gene regulating chlorophyll synthesis and adaptation to diverse stresses in watermelon. ClBCM：一个调控西瓜叶绿素合成和适应不同胁迫的EMS突变基因。

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Molecular Horticulture Pub Date : 2026-06-01 DOI: 10.1186/s43897-025-00223-6

Taohong Fang, Zhen Zhuang, Mingxia Zhao, Xiao Chu, Jingsheng Tan, Xinyang Xu, Rui Cheng, Yi Zhang, Xu Wang, Yunbi Xu, Xingping Zhang, Yun Deng

引用次数: 0

Chromosome-level genome assembly of Tamarindus indica provides new insights into the evolution of triterpenes and tartaric acid biosynthetic pathway. 柽柳染色体水平的基因组组装为三萜和酒石酸生物合成途径的进化提供了新的见解。

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Molecular Horticulture Pub Date : 2026-05-13 DOI: 10.1186/s43897-025-00222-7

Mitali Singh, Manohar S Bisht, Abhijith M G, Shruti Mahajan, Vineet K Sharma

{"title":"Chromosome-level genome assembly of Tamarindus indica provides new insights into the evolution of triterpenes and tartaric acid biosynthetic pathway.","authors":"Mitali Singh, Manohar S Bisht, Abhijith M G, Shruti Mahajan, Vineet K Sharma","doi":"10.1186/s43897-025-00222-7","DOIUrl":"10.1186/s43897-025-00222-7","url":null,"abstract":"Tamarindus indica is the sole member of the genus Tamarindus of the Leguminosae family. It is a multipurpose horticultural plant, with every part of the plant finding importance in food, medicine, and other industries. To gain an understanding of genome structure and evolution, we reported the first high-quality genome assembly of T. indica anchored to 12 chromosomes with an N50 of 56.6 Mb. Supported by comprehensive transcriptome data, we reported 48,867 protein-coding genes. Through phylogenetic and evolutionary analysis, we uncovered an independent whole-genome duplication event in T. indica and highlighted the expression divergence of segmentally duplicated genes and their role in the better adaptivity of the plant. Further, we observed a high expansion of the terpene cyclase mutase (oxidosqualene cyclase, OSCs) gene family and identified nine OSCs and their putative functions in T. indica. In addition, by employing integrated genomic, transcriptomic, and metabolomic analysis, we identified the putative L-Idonate dehydrogenase (L-IDH) gene in T. indica and provided evidence about its possible role in the accumulation of high tartaric acid content in this plant. Our study thus provides an important resource for future genetic and biotechnological studies to understand essential pathways and assist breeding programs for trait enhancements.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13169614/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147933978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PsGA2ox2 is a novel target of miR159 involved in endodormancy regulation in tree peony (Paeonia suffruticosa). PsGA2ox2是miR159参与牡丹内休眠调控的新靶点。

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Molecular Horticulture Pub Date : 2026-05-12 DOI: 10.1186/s43897-025-00220-9

Wenyu Gai, Xinyu Wang, Yongli Zhao, Tao Zhang, Yanchao Yuan, Chunying Liu, Shupeng Gai, Yuxi Zhang

{"title":"PsGA2ox2 is a novel target of miR159 involved in endodormancy regulation in tree peony (Paeonia suffruticosa).","authors":"Wenyu Gai, Xinyu Wang, Yongli Zhao, Tao Zhang, Yanchao Yuan, Chunying Liu, Shupeng Gai, Yuxi Zhang","doi":"10.1186/s43897-025-00220-9","DOIUrl":"10.1186/s43897-025-00220-9","url":null,"abstract":"In deciduous woody plants, buds enter endodormancy in late autumn, and endodormancy release (EDR) is a essential for regrowth. In tree peony, endodormancy is a major obstacle to forcing culture, an important component of its industry. Both chilling accumulation and gibberellin acid (GA) application can contribute to break dormancy and facilitate bud sprouting and flowering under appropriate conditions. PsmiR159b is one of the most abundant miRNAs in dormant buds, targeting PsMYB65 to inhibit bud EDR. Here, PsGA2ox2 was identified as a novel target of PsmiR159b according to expression data and genetic analysis. PsGA2ox2 expression was up-regulated by prolonged chilling, following with an increase of gibberellic acid 34 (GA34), a product of deactivated GA4 by GA2-oxidase. Silencing of PsGA2ox2 accelerated budbreak, accompanied by the increase of GA1 and GA4, downregulation of PsABI5, PsSVP, and upregulation of PsEBB1, PsEBB3, and PsCYCD. The content of GA4 was downregulated in STTM159b buds, whereas increased in MIR159b-OE buds. In addition, the ectopic expression of PsGA2ox2 in Arabidopsis dramatically delayed seed germination and inhibited plant growth. These findings indicated a dual-target regulatory role for PsmiR159b in regulating GA homeostasis, and also revealed a PsmiR159b-PsGA2ox2 module that influenced EDR in tree peony through bioactive GA level regulation.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13162426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147875991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic control of tomato fruit cracking through SlPMEI27-dependent pectin modification. 依赖slpmei27的果胶改性对番茄果实开裂的遗传控制

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Molecular Horticulture Pub Date : 2026-05-11 DOI: 10.1186/s43897-025-00219-2

Fanyue Meng, Haonan Qi, Zhen Ding, Peiwen Wang, Xiuling Chen, Yang Liu, Chao Gong, Mozhen Cheng, Aoxue Wang

{"title":"Genetic control of tomato fruit cracking through SlPMEI27-dependent pectin modification.","authors":"Fanyue Meng, Haonan Qi, Zhen Ding, Peiwen Wang, Xiuling Chen, Yang Liu, Chao Gong, Mozhen Cheng, Aoxue Wang","doi":"10.1186/s43897-025-00219-2","DOIUrl":"10.1186/s43897-025-00219-2","url":null,"abstract":"Fruit cracking severely affects tomato quality and yield. By utilizing genetic transformation, knockout of the SlPMEI27 gene can enhance the activity of pectin methylesterases (PMEs). Here, by conducting an immunofluorescence analysis, we observed that the pectin methylation rate decreased by approximately 46% (versus approximately 70% in the overexpression line), while calcium-mediated pectin cross-linking enhancement increased fruit hardness by 1.8-fold. In contrast, SlPMEI27 overexpression inhibits PME activity, resulting in highly methyl-esterified pectin. Transmission electron microscopy analysis demonstrated that SlPMEI27 overexpression significantly reduced cell-to-cell adhesion and increased intercellular space expansion. These factors contribute significantly to susceptibility to cracking. Additional evidence, such as enzyme activity and the expression levels of related genes, shows that the interaction between SlPMEI27 and SlGAUT7 regulates pectin modification and biosynthesis. Transcriptomic analysis revealed that SLPMEI27 is associated with altered expression of downstream cell wall hydrolases, such as pectate/pectin lyases (PL), polygalacturonases (PG), and β-galactosidases (β-Gal), as well as ripening-related transcription factors (bHLH, ERF, and MYB). This study demonstrates that SlPMEI27 regulates cracking by directly modulating pectin methylesterification and cell wall mechanical properties. These findings establish pectin methylesterification status as a crucial determinant of cell wall integrity during fruit development, offering a theoretical foundation for breeding crack-resistant tomatoes.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13159324/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147869554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cold storage delays peach fruit softening via m⁶A reader PpYTHDFE1 liquid-liquid phase separation-mediated degradation of cell wall-loosening transcript PpEXP3. 低温贮藏通过m6A读取器PpYTHDFE1液-液相分离介导的细胞壁疏松转录物PpEXP3降解延缓桃果果软化。

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Molecular Horticulture Pub Date : 2026-05-09 DOI: 10.1186/s43897-025-00218-3

Hanqing Wang, Hanxiao Bian, Xubin Wu, Kunsong Chen, Donald Grierson, Bo Zhang

{"title":"Cold storage delays peach fruit softening via m6A reader PpYTHDFE1 liquid-liquid phase separation-mediated degradation of cell wall-loosening transcript PpEXP3.","authors":"Hanqing Wang, Hanxiao Bian, Xubin Wu, Kunsong Chen, Donald Grierson, Bo Zhang","doi":"10.1186/s43897-025-00218-3","DOIUrl":"10.1186/s43897-025-00218-3","url":null,"abstract":"N6-methyladenosine (m6A), the most prevalent epitranscriptomic modification in plant mRNAs, functions as a critical regulatory layer governing environmental response. Refrigeration reduces transcripts of cell wall enzymes to delay softening, however whether m6A participated in this progress remains unclear. Here, we identified a cold-inducible m6A reader PpYTHDFE1 as a regulator that stabilizes peach (Prunus persica L.) fruit firmness. Epitranscriptomic analysis revealed that firmness maintenance correlates with reduced levels of m6A-modified transcripts such as expansin PpEXP3, whose overexpressing accelerates fruit softening. Both in vitro RNA-EMSA and in vivo RIP-qPCR assays demonstrate that PpYTHDFE1 recognizes m6A-modified PpEXP3 transcripts. Transcription inhibition assays further confirm that PpYTHDFE1 accelerates the degradation of PpEXP3 through RNA-protein interaction rather than transcriptional modulation. Mechanistically, PpYTHDFE1 participates in liquid-liquid phase separation as evidenced by fluorescence recovery after photobleaching (FRAP) assays, facilitating PpEXP3 degradation. Furthermore, overexpression of PpYTHDFE1 decreases PpEXP3 mRNA levels, while silencing PpYTHDFE1 leads to PpEXP3 transcript accumulation. A significantly negative correlation was observed between PpYTHDFE1 transcripts and firmness during fruit cold storage. Fruit firmness is increased by 44% in peach and 45% in tomato upon PpYTHDFE1 overexpressing. Our findings provide molecular insights into RNA modification-based strategies to reduce postharvest losses and develop cold-resilient horticultural crops.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13156859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147856728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Convergent roles of BcGUN4.1, BcSG1, BcCHLH, and BcTPR4 in regulating the leaf greenness of non-heading Chinese cabbage. BcGUN4.1、BcSG1、BcCHLH和BcTPR4在无头大白菜叶片绿度调控中的趋同作用

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Molecular Horticulture Pub Date : 2026-05-08 DOI: 10.1186/s43897-025-00216-5

Aimei Bai, Li Gong, Xinya Wang, Huanhuan Xu, Zhenzhi Zhu, Changwei Zhang, Tongkun Liu, Xilin Hou, Ying Li

{"title":"Convergent roles of BcGUN4.1, BcSG1, BcCHLH, and BcTPR4 in regulating the leaf greenness of non-heading Chinese cabbage.","authors":"Aimei Bai, Li Gong, Xinya Wang, Huanhuan Xu, Zhenzhi Zhu, Changwei Zhang, Tongkun Liu, Xilin Hou, Ying Li","doi":"10.1186/s43897-025-00216-5","DOIUrl":"10.1186/s43897-025-00216-5","url":null,"abstract":"Non-heading Chinese cabbage (NHCC) extensively cultivated as a leafy vegetable in China, displays diverse leaf coloration, with green being an essential trait. Leaf greenness, determined by chlorophyll and carotenoid contents, is a crucial visual characteristic, and understanding its genetic and molecular mechanisms is essential for breeding. This study identified two highly homologous BcGUN4 genes (BcGUN4.1 and BcGUN4.2) in NHCC. BcGUN4.1 exhibited localization to chloroplasts and cell membranes, positively regulating pigment accumulation. Similarly, BcSG1 and BcCHLH were detected in chloroplasts and cell membranes, co-localizing with BcGUN4.1. Notably, BcGUN4.1 interacted with BcSG1 and BcCHLH in chloroplasts, while BcSG1 functioned as a positive regulator of pigmentation. Moreover, BcGUN4.1, BcSG1, and BcCHLH interacted with BcTPR4, and BcSG1 directly bound to BcCHLH, forming a tetrameric complex in chloroplasts. BcTPR4 contributed to pigment accumulation. Additionally, BcSG1 and BcTPR4 enhanced BcGUN4.1-BcCHLH complex activity. These findings established that BcGUN4.1, BcSG1, BcCHLH, and BcTPR4 cooperatively regulate leaf greenness in NHCC, offering novel insights into the molecular mechanisms underlying this trait.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13154631/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LncRNA7503 decreases peach (Prunus persica) branch number and angle by inducing pre-miR395a degradation and reducing bioactive BR content. LncRNA7503通过诱导pre-miR395a降解，降低生物活性BR含量，降低桃树枝数和枝角。

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Molecular Horticulture Pub Date : 2026-05-07 DOI: 10.1186/s43897-025-00215-6

Xiaobei Wang, Hefang Xie, Wan Pei, Ruixian Shen, Yajia Zhang, Junjie Zhang, Xiaodong Lian, Haipeng Zhang, Xianbo Zheng, Nan Hou, Lei Wang, Jun Cheng, Wei Wang, Langlang Zhang, Xia Ye, Jidong Li, Jiancan Feng, Bin Tan

{"title":"LncRNA7503 decreases peach (Prunus persica) branch number and angle by inducing pre-miR395a degradation and reducing bioactive BR content.","authors":"Xiaobei Wang, Hefang Xie, Wan Pei, Ruixian Shen, Yajia Zhang, Junjie Zhang, Xiaodong Lian, Haipeng Zhang, Xianbo Zheng, Nan Hou, Lei Wang, Jun Cheng, Wei Wang, Langlang Zhang, Xia Ye, Jidong Li, Jiancan Feng, Bin Tan","doi":"10.1186/s43897-025-00215-6","DOIUrl":"10.1186/s43897-025-00215-6","url":null,"abstract":"Peach (Prunus persica L.) branch number and angle significantly influence orchard management efficiency, fruit yield, and quality. However, few reports exist on genes regulating both traits characteristics. This study utilized miRNA sequencing to identify PpCYP734A14, one of four BR pathway-related genes targeted by differentially expressed miRNAs, as a participant in BR metabolism with elevated expression in pillar-type peach cultivar Zhaoshouhong (ZSH). Transient expression analyses confirmed that miR395a-3p targets PpCYP734A14. The overexpression of pre-miR395a led to significant reduction in PpCYP734A14 mRNA levels and increased bioactive BR content, resulting in enhanced branching and branch angle. Conversely, miR395a-3p silencing produced opposite effects. LncRNA-miRNA-mRNA network analysis revealed that differentially expressed lncRNA7503 reduced mature miR395a-3p levels by promoting the posttranscriptional degradation of pre-miR395a, subsequently increasing PpCYP734A14 expression. These findings implicate PpCYP734A14 in the regulation of both branch number and angle through a lncRNA7503-miR395a-3p mechanism. This pathway presents valuable molecular tools for genetic improvement of tree architecture and facilitates labor-saving cultivation approaches.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13151148/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epitranscriptomic profiling of cytosine N4 acetylation (ac⁴C) in Solanum lycopersicum and dynamic changes under heat stress condition. 番茄茄胞嘧啶N4乙酰化（ac4C）的表转录组学分析及热胁迫下的动态变化

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Molecular Horticulture Pub Date : 2026-05-06 DOI: 10.1186/s43897-025-00214-7

Wanhong Zhang, Yubing Jiao, Yanxiao Bu, Lili Shen, Yingwen Wang, Ying Li, Binna Lv, Qi Huang, Yansong Xiao, Tianbo Liu, Jinguang Yang

{"title":"Epitranscriptomic profiling of cytosine N4 acetylation (ac4C) in Solanum lycopersicum and dynamic changes under heat stress condition.","authors":"Wanhong Zhang, Yubing Jiao, Yanxiao Bu, Lili Shen, Yingwen Wang, Ying Li, Binna Lv, Qi Huang, Yansong Xiao, Tianbo Liu, Jinguang Yang","doi":"10.1186/s43897-025-00214-7","DOIUrl":"10.1186/s43897-025-00214-7","url":null,"abstract":"N4-acetylcytidine (ac4C), which is an evolutionarily conserved RNA modification in eukaryotes, functions as a critical epitranscriptomic regulator that enhances mRNA stability and translation efficiency. In Arabidopsis thaliana and Oryza sativa, ac4C has been characterized as a modulator of transcriptome homeostasis that functions by regulating translational efficiency and maintaining RNA structure; however, the distribution patterns and biological functions of ac4C in other plant species remain largely unexplored. A transcriptome-wide map of the ac4C of Solanum lycopersicum revealed conserved features of plant ac4C modification, with preferential enrichment in mRNA translation initiation and termination regions as well as high conservation of modification motifs and functional categories of target genes across species. Notably, heat stress triggered global ac4C hyperacetylation, and hypermodified genes were enriched in transcripts encoding photosynthesis and thermotolerance-associated regulators. Multiomics integration further revealed that hyperacetylated and upregulated genes were significantly enriched in stress-response pathways, accompanied by elevated transcription levels. Silencing of SLNAT10 resulted in reduced stability of highly acetylated transcripts under heat stress conditions. These findings reveal that RNA acetylation is synchronized with posttranscriptional regulation that promotes transcriptional reprogramming to establish heat tolerance, providing a mechanism for the epitranscriptomic control of environmental adaptation in plants.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13147876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147843550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carotenoid aggregates negatively impact chlorophyll levels and disrupt chloroplast development in peaches. 类胡萝卜素聚集体对桃的叶绿素水平产生负面影响，破坏叶绿体发育。

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Molecular Horticulture Pub Date : 2026-04-10 DOI: 10.1186/s43897-025-00213-8

Pengfei Wang, Xulei Zhao, Lipeng Liu, Caizhen Gao, Kaijie Zhu, Jiahui Wang, Yan Han, Xueying Zhang, Haijiang Chen, Li Li, Hongbo Cao

{"title":"Carotenoid aggregates negatively impact chlorophyll levels and disrupt chloroplast development in peaches.","authors":"Pengfei Wang, Xulei Zhao, Lipeng Liu, Caizhen Gao, Kaijie Zhu, Jiahui Wang, Yan Han, Xueying Zhang, Haijiang Chen, Li Li, Hongbo Cao","doi":"10.1186/s43897-025-00213-8","DOIUrl":"10.1186/s43897-025-00213-8","url":null,"abstract":"Peach (Prunus persica L. Batsch) is among the most economically important fruit tree crops. Carotenoids in peach fruit have been intensively studied because of their relationship with fruit color and nutritional value. High carotenoid accumulation is generally a desirable trait in fruits and is associated with their aggregation in plastids. However, the understanding of the functions of carotenoid aggregates is still limited, especially with respect to their effects on chromoplast differentiation. The present study revealed that carotenoid aggregates have significant biological effects on peach. Lycopene and β-carotene aggregates promoted chlorophyll degradation and thylakoid membrane remodeling, leading to the differentiation of chloroplasts into chromoplasts in peach fruit and callus. However, the phytoene and phytofluene did not produce this effect in peach fruit. Interestingly, during lycopene and β-carotene aggregation, the stay-green-like (SGRL) gene was significantly activated, and further confirmed that it was an important factor affecting peach chlorophyll degradation and carotenoid accumulation. Furthermore, molecular dynamics analysis confirmed that lycopene and β-carotene had a greater effect on membrane thickness and rigidity than phytoene. This study contributes to a deeper understanding of peach carotenoid aggregates and their biological effects, confirming the effectiveness of the transgenic peach callus system.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13067727/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147646800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-quality genome assembly of Aglaia odorata reveals evolution, terpenes diversity and abundance in Meliaceae. 高质量的香缕草基因组组装揭示了香缕草科植物萜类的进化、多样性和丰度。

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Molecular Horticulture Pub Date : 2026-04-09 DOI: 10.1186/s43897-025-00212-9

Zhiyu Chen, Xingyu Yang, Tianyu Yang, Xin Yin, Danni Yang, Xuefei Yang, Yunqiang Yang, Yongping Yang

{"title":"High-quality genome assembly of Aglaia odorata reveals evolution, terpenes diversity and abundance in Meliaceae.","authors":"Zhiyu Chen, Xingyu Yang, Tianyu Yang, Xin Yin, Danni Yang, Xuefei Yang, Yunqiang Yang, Yongping Yang","doi":"10.1186/s43897-025-00212-9","DOIUrl":"10.1186/s43897-025-00212-9","url":null,"abstract":"Meliaceae species are known for producing diverse metabolites, yet the role of whole-genome duplication (WGD) in shaping this diversity remains unclear. In this study, we analyzed volatile metabolites in Azadirachta indica, Toona ciliata and Aglaia odorata, finding terpenes to be most abundant in A. odorata. A high-quality genome assembly of A. odorata revealed lineage-specific whole-genome triplication (WGT), the first reported in Meliaceae. Comparative genomics indicated extensive chromosomal rearrangements, biased gene retention, gene expression, and long terminal repeat (LTR) insertions during the evolution of subgenomes in Meliaceae, with A. odorata showing subgenome dominance. Despite a reduction in terpene synthase (TPS) gene copies, the specifically highly expression of AodTPS25, AodTPS21, AodTPS24 and AodTPS26 in A. odorata could be crucial for its terpene abundance and diversity through transcriptomics and enzyme assays. Moreover, the ethylene response factor AodERF27 was found to activate AodTPS26, linking transcriptional regulation to terpene diversity. These findings uncovered the genomic and molecular mechanisms in Meliaceae.","PeriodicalId":29970,"journal":{"name":"Molecular Horticulture","volume":"6 1","pages":""},"PeriodicalIF":10.0,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13063475/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147640069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0