ACS Materials Au最新文献

{"title":"Platelet Responses to Urethane Dimethacrylate-Based Bone Cements Containing Monocalcium Phosphate/ε-Polylysine: Role of ε-Polylysine in <i>In Vitro</i> Wound Healing Induced by Platelet-Derived Growth Factor-BB.","authors":"Phatchanat Klaihmon, Piyarat Sungkhaphan, Boonlom Thavornyutikarn, Setthawut Kitpakornsanti, Praphasri Septham, Anne Young, Chanchao Lorthongpanich, Wanida Janvikul, Weerachai Singhatanadgit","doi":"10.1021/acsmaterialsau.4c00143","DOIUrl":"https://doi.org/10.1021/acsmaterialsau.4c00143","url":null,"abstract":"<p><p>Platelets play a pivotal role in initiating bone fracture healing. However, the interaction between platelets and bone cements used for fracture repair remains relatively unexplored. This study investigated the platelet response to recently developed urethane dimethacrylate-based bone cements containing 8% (w/w) monocalcium phosphate monohydrate (MCPM) and/or 5% (w/w) ε-polylysine (PLS). All experimental bone cements achieved final monomer conversions of 75-78%, compared with the 86% conversion of the commercial PMMA bone cement Kyphon. The MCPM and PLS microparticles, varying in size, were dispersed within the glass-filler-incorporated polymer matrix. In contrast to Kyphon, all experimental cements exhibited significantly smoother and more hydrophilic surfaces. Bone cements incorporating PLS, with or without MCPM, effectively activated platelets by inducing cellular adhesion, aggregation, and extracellular-signal-regulated kinase (ERK) activation, comparable to Kyphon. Flow cytometry analysis demonstrated a statistically significant increase in CD62P-positive platelets following exposure to PLS-incorporated bone cements and exogenously administered PLS in a concentration-dependent manner, but not with Kyphon. A wound healing assay revealed a 2-fold enhancement in wound closure within 24 h and exceeding 85% at 48 h by bone cements containing PLS, with or without MCPM, and Kyphon. Notably, platelet-derived growth factor BB (PDGF-BB) secretion was significantly elevated, specifically after platelet exposure to PLS-incorporated bone cements, a phenomenon not observed with Kyphon. Interestingly, PDGF-BB neutralization attenuated wound closure induced by the PLS-incorporated bone cements. In conclusion, the urethane dimethacrylate-based bone cements containing PLS demonstrated a significant enhancement in platelet activation and PDGF-BB secretion, which, at least partly, enhanced <i>in vitro</i> wound closure. The results suggest that PDGF-BB plays a crucial role in the PLS-mediated enhancement of wound healing in these bone cements.</p>","PeriodicalId":29798,"journal":{"name":"ACS Materials Au","volume":"5 2","pages":"339-352"},"PeriodicalIF":5.7,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907285/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Platelet Responses to Urethane Dimethacrylate-Based Bone Cements Containing Monocalcium Phosphate/ε-Polylysine: Role of ε-Polylysine in In Vitro Wound Healing Induced by Platelet-Derived Growth Factor-BB","authors":"Phatchanat Klaihmon,&nbsp;Piyarat Sungkhaphan,&nbsp;Boonlom Thavornyutikarn,&nbsp;Setthawut Kitpakornsanti,&nbsp;Praphasri Septham,&nbsp;Anne Young,&nbsp;Chanchao Lorthongpanich,&nbsp;Wanida Janvikul* and Weerachai Singhatanadgit*,&nbsp;","doi":"10.1021/acsmaterialsau.4c0014310.1021/acsmaterialsau.4c00143","DOIUrl":"https://doi.org/10.1021/acsmaterialsau.4c00143https://doi.org/10.1021/acsmaterialsau.4c00143","url":null,"abstract":"<p >Platelets play a pivotal role in initiating bone fracture healing. However, the interaction between platelets and bone cements used for fracture repair remains relatively unexplored. This study investigated the platelet response to recently developed urethane dimethacrylate-based bone cements containing 8% (w/w) monocalcium phosphate monohydrate (MCPM) and/or 5% (w/w) ε-polylysine (PLS). All experimental bone cements achieved final monomer conversions of 75–78%, compared with the 86% conversion of the commercial PMMA bone cement Kyphon. The MCPM and PLS microparticles, varying in size, were dispersed within the glass-filler-incorporated polymer matrix. In contrast to Kyphon, all experimental cements exhibited significantly smoother and more hydrophilic surfaces. Bone cements incorporating PLS, with or without MCPM, effectively activated platelets by inducing cellular adhesion, aggregation, and extracellular-signal-regulated kinase (ERK) activation, comparable to Kyphon. Flow cytometry analysis demonstrated a statistically significant increase in CD62P-positive platelets following exposure to PLS-incorporated bone cements and exogenously administered PLS in a concentration-dependent manner, but not with Kyphon. A wound healing assay revealed a 2-fold enhancement in wound closure within 24 h and exceeding 85% at 48 h by bone cements containing PLS, with or without MCPM, and Kyphon. Notably, platelet-derived growth factor BB (PDGF-BB) secretion was significantly elevated, specifically after platelet exposure to PLS-incorporated bone cements, a phenomenon not observed with Kyphon. Interestingly, PDGF-BB neutralization attenuated wound closure induced by the PLS-incorporated bone cements. In conclusion, the urethane dimethacrylate-based bone cements containing PLS demonstrated a significant enhancement in platelet activation and PDGF-BB secretion, which, at least partly, enhanced <i>in vitro</i> wound closure. The results suggest that PDGF-BB plays a crucial role in the PLS-mediated enhancement of wound healing in these bone cements.</p>","PeriodicalId":29798,"journal":{"name":"ACS Materials Au","volume":"5 2","pages":"339–352 339–352"},"PeriodicalIF":5.7,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmaterialsau.4c00143","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143591245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Biocompatible, UV and NIR Excitable Nanoparticles with Multiwavelength Emission and Enhanced Colloidal Stability","authors":"Egle Ezerskyte,&nbsp;Greta Butkiene,&nbsp;Arturas Katelnikovas and Vaidas Klimkevicius*,&nbsp;","doi":"10.1021/acsmaterialsau.4c0015110.1021/acsmaterialsau.4c00151","DOIUrl":"https://doi.org/10.1021/acsmaterialsau.4c00151https://doi.org/10.1021/acsmaterialsau.4c00151","url":null,"abstract":"<p >The development of functional nanoprobes for biomedical applications is highly important in the field of modern nanotechnology. Due to strict requirements, such as the ability to be excited using irradiation, which allows deep tissue penetration, nonblinking behavior, and good optical and colloidal stability, the choice of nanoparticles is limited, and their synthesis is challenging. Among all of the functional nanoprobes for biomedical purposes, upconverting nanoparticles, especially those with more complex architectures (e.g., core–shell or core–shell–shell), are the most promising candidates. This study demonstrates advanced synthetic routes for constructing biocompatible nanoprobes with tunable optical properties and colloidal stability. The core–shell–shell architecture of the nanoprobes allows excitation from at least four sources, such as 272 and 394 nm of near-ultraviolet (near-UV) irradiation and 980 and 808 nm near-infrared (NIR) lasers. Furthermore, Gd-matrix-based nanoprobes doped with lanthanide ions (Nd³⁺, Yb³⁺, Tm³⁺, and Eu³⁺) are known for their paramagnetic properties for magnetic resonance imaging (MRI) imaging as well as upconversion luminescence with diverse emission bands across the entire visible spectrum. This feature is highly desirable for photodynamic therapy applications, as the upconversion emission of the proposed nanoprobes could overlap with the absorption band of commonly used photosensitizers and could potentially result in an efficient energy transfer process and enhanced generation of reactive oxygen species or singlet oxygen.</p>","PeriodicalId":29798,"journal":{"name":"ACS Materials Au","volume":"5 2","pages":"353–364 353–364"},"PeriodicalIF":5.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmaterialsau.4c00151","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143591386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Luminescent Oxygen Sensor with Self-Sterilization Properties Based on Platinum(II)octaethylporphyrin in Polymeric Nanofibers","authors":"Pavel Ludačka,&nbsp;Vojtěch Liška,&nbsp;Jan Sýkora,&nbsp;Pavel Kubát and Jiří Mosinger*,&nbsp;","doi":"10.1021/acsmaterialsau.4c0013710.1021/acsmaterialsau.4c00137","DOIUrl":"https://doi.org/10.1021/acsmaterialsau.4c00137https://doi.org/10.1021/acsmaterialsau.4c00137","url":null,"abstract":"<p >Optical sensors based on the quenching of the luminescence of platinum(II)octaethylporphyrin (PtOEP) encapsulated in nanofiber polymeric membranes were prepared by electrospinning. The samples were characterized using scanning electron microscopy, confocal luminescence microscopy, absorption spectroscopy, and steady-state and time-resolved luminescence techniques. The properties of the sensors were changed by the selection of different polymeric membranes using polycaprolactone, polystyrene, polyurethane Tecophilic, and poly(vinylidene fluoride-<i>co</i>-hexafluoropropylene) polymers. Among them, biodegradable and biocompatible sensors prepared from polycaprolactone with a high oxygen diffusion coefficient exhibited a fast response time (0.37 s), recovery time (0.58 s), high sensitivity (maximum <i>I</i>_<i>0</i><i>/I</i> ratio = 52), reversible luminescent response, and linear Stern–Volmer quenching over the whole range of oxygen contents in both the gas atmosphere and aqueous media. Moreover, the proposed sensors exhibited high antibacterial properties, resulting in self-sterilization character of the membrane surface due to the photogeneration of singlet oxygen. This dual character can find application in the biomedical field, where both properties (oxygen sensing and self-sterilization) can be acquired from the same material.</p>","PeriodicalId":29798,"journal":{"name":"ACS Materials Au","volume":"5 2","pages":"331–338 331–338"},"PeriodicalIF":5.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmaterialsau.4c00137","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143591387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Luminescent Oxygen Sensor with Self-Sterilization Properties Based on Platinum(II)octaethylporphyrin in Polymeric Nanofibers.","authors":"Pavel Ludačka, Vojtěch Liška, Jan Sýkora, Pavel Kubát, Jiří Mosinger","doi":"10.1021/acsmaterialsau.4c00137","DOIUrl":"https://doi.org/10.1021/acsmaterialsau.4c00137","url":null,"abstract":"<p><p>Optical sensors based on the quenching of the luminescence of platinum(II)octaethylporphyrin (PtOEP) encapsulated in nanofiber polymeric membranes were prepared by electrospinning. The samples were characterized using scanning electron microscopy, confocal luminescence microscopy, absorption spectroscopy, and steady-state and time-resolved luminescence techniques. The properties of the sensors were changed by the selection of different polymeric membranes using polycaprolactone, polystyrene, polyurethane Tecophilic, and poly(vinylidene fluoride-<i>co</i>-hexafluoropropylene) polymers. Among them, biodegradable and biocompatible sensors prepared from polycaprolactone with a high oxygen diffusion coefficient exhibited a fast response time (0.37 s), recovery time (0.58 s), high sensitivity (maximum <i>I</i> _<i>0</i> <i>/I</i> ratio = 52), reversible luminescent response, and linear Stern-Volmer quenching over the whole range of oxygen contents in both the gas atmosphere and aqueous media. Moreover, the proposed sensors exhibited high antibacterial properties, resulting in self-sterilization character of the membrane surface due to the photogeneration of singlet oxygen. This dual character can find application in the biomedical field, where both properties (oxygen sensing and self-sterilization) can be acquired from the same material.</p>","PeriodicalId":29798,"journal":{"name":"ACS Materials Au","volume":"5 2","pages":"331-338"},"PeriodicalIF":5.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Biocompatible, UV and NIR Excitable Nanoparticles with Multiwavelength Emission and Enhanced Colloidal Stability.","authors":"Egle Ezerskyte, Greta Butkiene, Arturas Katelnikovas, Vaidas Klimkevicius","doi":"10.1021/acsmaterialsau.4c00151","DOIUrl":"https://doi.org/10.1021/acsmaterialsau.4c00151","url":null,"abstract":"<p><p>The development of functional nanoprobes for biomedical applications is highly important in the field of modern nanotechnology. Due to strict requirements, such as the ability to be excited using irradiation, which allows deep tissue penetration, nonblinking behavior, and good optical and colloidal stability, the choice of nanoparticles is limited, and their synthesis is challenging. Among all of the functional nanoprobes for biomedical purposes, upconverting nanoparticles, especially those with more complex architectures (e.g., core-shell or core-shell-shell), are the most promising candidates. This study demonstrates advanced synthetic routes for constructing biocompatible nanoprobes with tunable optical properties and colloidal stability. The core-shell-shell architecture of the nanoprobes allows excitation from at least four sources, such as 272 and 394 nm of near-ultraviolet (near-UV) irradiation and 980 and 808 nm near-infrared (NIR) lasers. Furthermore, Gd-matrix-based nanoprobes doped with lanthanide ions (Nd³⁺, Yb³⁺, Tm³⁺, and Eu³⁺) are known for their paramagnetic properties for magnetic resonance imaging (MRI) imaging as well as upconversion luminescence with diverse emission bands across the entire visible spectrum. This feature is highly desirable for photodynamic therapy applications, as the upconversion emission of the proposed nanoprobes could overlap with the absorption band of commonly used photosensitizers and could potentially result in an efficient energy transfer process and enhanced generation of reactive oxygen species or singlet oxygen.</p>","PeriodicalId":29798,"journal":{"name":"ACS Materials Au","volume":"5 2","pages":"353-364"},"PeriodicalIF":5.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Essential Role of Well-Defined Materials in Assessing Electrocatalyst Structure and Function","authors":"Anthony Shoji Hall*,&nbsp;","doi":"10.1021/acsmaterialsau.4c0010510.1021/acsmaterialsau.4c00105","DOIUrl":"https://doi.org/10.1021/acsmaterialsau.4c00105https://doi.org/10.1021/acsmaterialsau.4c00105","url":null,"abstract":"<p >Advancements in heterogeneous electrocatalysis are essential for the transition to a sustainable future. However, current catalysts often face limitations in activity, selectivity, stability, and cost-effectiveness, highlighting the need for a detailed understanding of catalyst behavior to construct rational design strategies. This perspective focuses on the role of well-defined materials, particularly ordered intermetallics, in enhancing catalytic performance. Ordered intermetallic materials enable precise control over geometric and electronic properties, which are critical for tailoring reactivity. Their structured atomic configurations not only allow for rational control of catalytic activity but also integrate seamlessly with computational models, facilitating the design of improved materials. Additionally, we examine future research directions for intermetallics in electrocatalysis, identifying opportunities for significant breakthroughs in the field.</p>","PeriodicalId":29798,"journal":{"name":"ACS Materials Au","volume":"5 2","pages":"231–238 231–238"},"PeriodicalIF":5.7,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmaterialsau.4c00105","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143590372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hallmarks of Polymersome Characterization.","authors":"Simon Matoori","doi":"10.1021/acsmaterialsau.4c00107","DOIUrl":"https://doi.org/10.1021/acsmaterialsau.4c00107","url":null,"abstract":"<p><p>Polymersomes have the potential to become the next generation of vesicular drug delivery systems. Their high chemical versatility and in certain cases higher membrane stability than liposomes raised the high hopes for polymersomes as a drug carrier, but the clinical translation has been slow. To jump-start translation, there is a need for meticulous characterization and reporting of key parameters of polymersome formulations. Regulatory authorities have provided valuable insights on critical quality attributes of liposomes in their guidance document on liposomal nanosimilars. Inspired by this guidance document, this Perspective proposes necessary characterization of polymersomes (hallmarks) regarding their chemical composition, physicochemical properties, drug release profile, stability, stimuli responsiveness, and pharmacokinetics and biodistribution.</p>","PeriodicalId":29798,"journal":{"name":"ACS Materials Au","volume":"5 2","pages":"223-230"},"PeriodicalIF":5.7,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143650628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hallmarks of Polymersome Characterization","authors":"Simon Matoori*,&nbsp;","doi":"10.1021/acsmaterialsau.4c0010710.1021/acsmaterialsau.4c00107","DOIUrl":"https://doi.org/10.1021/acsmaterialsau.4c00107https://doi.org/10.1021/acsmaterialsau.4c00107","url":null,"abstract":"<p >Polymersomes have the potential to become the next generation of vesicular drug delivery systems. Their high chemical versatility and in certain cases higher membrane stability than liposomes raised the high hopes for polymersomes as a drug carrier, but the clinical translation has been slow. To jump-start translation, there is a need for meticulous characterization and reporting of key parameters of polymersome formulations. Regulatory authorities have provided valuable insights on critical quality attributes of liposomes in their guidance document on liposomal nanosimilars. Inspired by this guidance document, this Perspective proposes necessary characterization of polymersomes (hallmarks) regarding their chemical composition, physicochemical properties, drug release profile, stability, stimuli responsiveness, and pharmacokinetics and biodistribution.</p>","PeriodicalId":29798,"journal":{"name":"ACS Materials Au","volume":"5 2","pages":"223–230 223–230"},"PeriodicalIF":5.7,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsmaterialsau.4c00107","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143590373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Essential Role of Well-Defined Materials in Assessing Electrocatalyst Structure and Function.","authors":"Anthony Shoji Hall","doi":"10.1021/acsmaterialsau.4c00105","DOIUrl":"https://doi.org/10.1021/acsmaterialsau.4c00105","url":null,"abstract":"<p><p>Advancements in heterogeneous electrocatalysis are essential for the transition to a sustainable future. However, current catalysts often face limitations in activity, selectivity, stability, and cost-effectiveness, highlighting the need for a detailed understanding of catalyst behavior to construct rational design strategies. This perspective focuses on the role of well-defined materials, particularly ordered intermetallics, in enhancing catalytic performance. Ordered intermetallic materials enable precise control over geometric and electronic properties, which are critical for tailoring reactivity. Their structured atomic configurations not only allow for rational control of catalytic activity but also integrate seamlessly with computational models, facilitating the design of improved materials. Additionally, we examine future research directions for intermetallics in electrocatalysis, identifying opportunities for significant breakthroughs in the field.</p>","PeriodicalId":29798,"journal":{"name":"ACS Materials Au","volume":"5 2","pages":"231-238"},"PeriodicalIF":5.7,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11907288/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143651098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}