Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry最新文献_第3页

BIOMARKERS OF OXIDANT STRESS IN BRONCHIAL ASTHMA 支气管哮喘中氧化应激的生物标志物

Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry Pub Date : 1900-01-01 DOI: 10.2298/JMB0604349C

V. Ćosić, I. Stanković, M. Rančić, L. Zvezdanović, S. Kundalić, V. Djordjević

{"title":"BIOMARKERS OF OXIDANT STRESS IN BRONCHIAL ASTHMA","authors":"V. Ćosić, I. Stanković, M. Rančić, L. Zvezdanović, S. Kundalić, V. Djordjević","doi":"10.2298/JMB0604349C","DOIUrl":"https://doi.org/10.2298/JMB0604349C","url":null,"abstract":"Summary: Oxidant stress is believed to contribute to both the initiation and development of bronchial asthma (BA). As such, the pathophysiology of BA is characterised by the large generation of reactive oxygen spe cies (ROS) and reactive nitrogen species (RNS), predominantly by inflammatory cells of asthmatic airways. These species play an important role in remodelling airways and in orchestrating the type of inflammatory response. Oxidants influence the specific balance of Th1/Th2 cytokines, and together with Th2-cytokines and Th2 induced cells can cause many of the features typical of asthma. They induce bronchoconstriction, mucus secretion, effects on airway vasculature, and increase airway responsiveness to several agonists. This review discusses the necessity of oxidant stress evaluation in BA using reliable biomarkers, which provide an appropriate tool for studying oxidant stress. It is desirable to determine prooxidants and antioxidant systemic and local parameters in lung specific media such as bronchoalveolar lavage (BAL), sputum, exhaled air and breath condensate. These airway biomarkers may be representative indicators which could show the processes occurring on the airway surface, the initial site of oxidation. Thus, the examination of biomarkers is essential for establishing the potential target for antioxidant supplementation that would be able to normalise the oxidant/ antioxidant imbalance.","PeriodicalId":287983,"journal":{"name":"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126539707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ALTERATION OF CIRCULATING INSULIN-LIKE GROWTH FACTORS IN PATIENTS INFECTED WITH BACTERIA HELICOBACTER PYLORI OR FRANCISELLA TULARENSIS 幽门螺杆菌或土拉弗朗西斯菌感染患者循环胰岛素样生长因子的改变

Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry Pub Date : 1900-01-01 DOI: 10.2298/JMH0204339B

I. Baričević, R. Nedic, Anna Nikolić-Judith, D. Marjanovic, E. Ristanovic, B. Lako

引用次数: 1

THE INFLUENCE OF ATOPY ON sICAM-1 SERUM LEVELS IN PATIENTS WITH ALLERGIC RHINITIS AND BRONCHIAL ASTHMA 特应性对变应性鼻炎和支气管哮喘患者血清中sICAM-1水平的影响

Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry Pub Date : 1900-01-01 DOI: 10.2298/JMH0404355P

A. Perić-Popadić, M. Bogic, Ž. Jovičić, S. Rašković, L. V. Tomic-Spiric, S. Kovačević, M. J. Bolpacic, J. Čolić

{"title":"THE INFLUENCE OF ATOPY ON sICAM-1 SERUM LEVELS IN PATIENTS WITH ALLERGIC RHINITIS AND BRONCHIAL ASTHMA","authors":"A. Perić-Popadić, M. Bogic, Ž. Jovičić, S. Rašković, L. V. Tomic-Spiric, S. Kovačević, M. J. Bolpacic, J. Čolić","doi":"10.2298/JMH0404355P","DOIUrl":"https://doi.org/10.2298/JMH0404355P","url":null,"abstract":"Summary: It has been shown that adhesive molecules are involved in inflammatory diseases of the lungs such as bronchial asthma. The purpose of the study was to measure and establish possible difference in serum levels of soluble ICAM-1 in 42 atopic patients (patients with allergic rhinitis and patients with bronchial asthma) in comparison with 28 patients without atopy (patients with asthma without rhinitis); whether there is a difference in sICAM-1 levels between groups of 26 patients with allergic rhinitis and asthma in comparison with group of 16 patients with allergic rhinitis only and also in comparison with 10 healthy controls. Results of the study have substantiated statistically significant difference in sICAM-1 levels between all groups of patients in comparison to healthy control, but no statistically significant difference in sICAM-1 levels between patients with and without atopy (Z=’1.738) or between patients with allergic rhinitis and bronchial asthma in comparison with group of patients with allergic rhinitis only (Z=0.00). ICAM-1 is an important marker of inflammation in patients with allergic rhinitis as well as in those with bronchial asthma. Atopic status does not influence differences in sICAM-1 levels. Although mean sICAM-1 levels were higher in patients with allergic rhinitis and bronchial asthma (312.71 ng/mL) in comparison with mean sICAM-1 levels in patients with allergic rhinitis only (279.69 ng/mL), no statistically significant difference was noted in sICAM-1 levels between these groups of subjects, i.e. asthma itself did not contribute to statistically significant increase of sICAM-1 levels.","PeriodicalId":287983,"journal":{"name":"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130905789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Diagnostic value of CSF proteins assay in differential diagnosis of CNS disease 脑脊液蛋白测定在中枢神经系统疾病鉴别诊断中的价值

Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry Pub Date : 1900-01-01 DOI: 10.2298/JMH0502107V

Dragana Vukosavljevic, L. Bumbaširević, A. Stefanović, M. Stojanov

引用次数: 0

THE EFFECT OF ZINC DIET ON DISTRIBUTION OF FATTY ACID IN BLOOD PLASMA CHYLOMICRONS 锌饮食对血浆乳糜微粒中脂肪酸分布的影响

Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry Pub Date : 1900-01-01 DOI: 10.2298/JMH0501015C

Aleksandra Cerović, I. Miletić, P. Blagojevic, S. Sobajic, M. Čolić, M. Vasiljevska, M. Poznanic

{"title":"THE EFFECT OF ZINC DIET ON DISTRIBUTION OF FATTY ACID IN BLOOD PLASMA CHYLOMICRONS","authors":"Aleksandra Cerović, I. Miletić, P. Blagojevic, S. Sobajic, M. Čolić, M. Vasiljevska, M. Poznanic","doi":"10.2298/JMH0501015C","DOIUrl":"https://doi.org/10.2298/JMH0501015C","url":null,"abstract":"Summary: The effect of dietary zinc on the distribution of fatty acids in blood plasma chylomicrons was studied. The experiment was performed on male Mongolian Gerbils, fed ad libitum for 3 weeks with standard diet, containing 8 or 38 mg of Zn per kg of food (low zinc diet group, LZ, and saturated zinc diet group, SZ, respectivelly). At the 21th day gerbils were given sunflower oil by gavage. After 2.5 hours, blood was collected from abdominal vein, and samples were pooled (five animals per one sample). Chylomicron fractions were isolated by ultracentrifugation and mass of dried chylomicrons was measured. Fatty acid composition was analyzed by gas-liquid chromatography. Significantly higher amount of chylomicrons in blood plasma of SZ than in LZ group was found. In chylomicrons, following fatty acids were found: 16:0, 16:1, 17:0, 17:1, 18:0, 18:1, 18:2, 18:3, 20:0, 21:0 and 20:4. The amount of individual fatty acids in chylomicrons in both groups was similar, except 20:4 where lower amount in SZ group was found. Zinc diet did not affect fatty acid distribution in chylomicrons of both groups. Animals fed with zinc saturated diet had higher amount of fatty acids in blood plasma. Observed results suggest that dietary zinc influences the quantity of fatty acids absorption but not its distribution in chylomicrons.","PeriodicalId":287983,"journal":{"name":"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127224757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

RAPID CHARACTERIZATION OF b-THALASSEMIA MUTATIONS BY REVERSE DOT BLOT AND ALLELE-SPECIFIC PCR ANALYSIS 利用反向点印迹和等位基因特异性PCR分析快速表征b-地中海贫血突变

Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry Pub Date : 1900-01-01 DOI: 10.2298/JMH0203283P

T. Pavlovic, J. Urosevic, T. Djureinovic, M. D. Janić, L. Krivokapić-Dokmanović

引用次数: 2

The use of biochemical markers for diagnosis of the acute coronary syndromes 生化指标在急性冠状动脉综合征诊断中的应用

Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry Pub Date : 1900-01-01 DOI: 10.2298/JMH0304289M

N. Majkić-Singh

{"title":"The use of biochemical markers for diagnosis of the acute coronary syndromes","authors":"N. Majkić-Singh","doi":"10.2298/JMH0304289M","DOIUrl":"https://doi.org/10.2298/JMH0304289M","url":null,"abstract":"It was recognized very early that the specificity of AST, LDH, and CK was not high for cardiac diseases, as injury to the liver and skeletal muscles and hemolysis produced falsely positive results. Thus the measurement of isoenzymes becomes important to improve the specificity of total enzyme measurements. Assays for CK and LD isoenzymes were initially developed using electrophoresis. Because this assay was time consuming and difficult to automate, imunooinhibition assays were soon developed. The development of immunoassay measurements enabled determination of the enzyme concentration which was not dependent on whether or not the enzyme was active. These \"mass assays\" also enabled the measurement of protein markers which are themselves not enzymes. This led to the investigation of many other markers for potential use in cardiac diseases. With changing clinical practices, cardiac markers are now needed to detect the presence of minor myocardial infarction in patients with unstable angina. Outcome studies have shown that patients with increased troponin are at high short-term risk for death and AMI. The success of new cardiac markers such as troponin is due to their high cardiac specificity and the existence of assays with low detection limits.","PeriodicalId":287983,"journal":{"name":"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122172640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The role of eosinophilic leukocytes in pathogenesis of bronchial asthma 嗜酸性白细胞在支气管哮喘发病机制中的作用

Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry Pub Date : 1900-01-01 DOI: 10.2298/JMH0404333V

D. Vučević, T. Radosavljević, G. Djordjević-Denić

引用次数: 1

Ecocute ecological disinfectant 生态消毒剂

Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry Pub Date : 1900-01-01 DOI: 10.2298/JMB0603263N

Ljiljana Nikolić-Bujanović, N. H. Popović, M. Simičić, M. Čekerevac, P. Rakin

引用次数: 5

LIPOPROTEIN METABOLISM ABNORMALITIES IN PATIENTS WITH CHRONIC RENAL INSUFFICIENCY 慢性肾功能不全患者的脂蛋白代谢异常

Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry Pub Date : 1900-01-01 DOI: 10.2298/JMH0204323J

Mirjana Jugovic, V. Spasojević-Kalimanovska, S. Spasić, Z. Nikolić

{"title":"LIPOPROTEIN METABOLISM ABNORMALITIES IN PATIENTS WITH CHRONIC RENAL INSUFFICIENCY","authors":"Mirjana Jugovic, V. Spasojević-Kalimanovska, S. Spasić, Z. Nikolić","doi":"10.2298/JMH0204323J","DOIUrl":"https://doi.org/10.2298/JMH0204323J","url":null,"abstract":"Summary: Patients with chronic renal insufficiency on hemodialysis develop lipoprotein abnormalities that may contribute to increased risk for atherosclerosis. The atherogenic risk for chronic renal insufficiency patients and dialysis treated patients was assessed by measuring total cholesterol, triglycerides, HDL-cholesterol, LDLcholesterol and calculating the ratio: TC/HDL-C and LDL-C/HDL-C. The examined group consisted of 18 cronic renal insufficiency patients and 60 patients on hemodialysis. The results were compared to a control group of 85 voluntary blood donors. Serum lipid parameters were examined by standard methods. All lipid parameters in hemodialysis patients were statistically different compared to the control group (p<0.05) while chronic renal insufficiency patients showed significant difference only in triglycerides and HDL-cholesterol. Hypertriglyceridemia was present in both examined groups of patients and HDL-cholesterol was lower within both groups. All calculated atherogenic ratios were higher for patients than the control group. Lipid parameters were compared between chronic renal insufficiency and hemodialysis patients but statistical significantly difference was obtained only for HDL-cholesterol (p<0.05). We conclude that increased values of triglycerides and lower HDL-cholesterol in chronic renal insufficiency patients contribute to high incidence of cardiovascular disease. Chronic renal insufficiency patients have impaired reverse cholesterol transport from peripheral cells to lipoproteins, decreased levels of HDL-cholesterol, hypertriglyceridemia prevalence of small, dense LDL and increased levels of potentially atherogenic remnant particles.","PeriodicalId":287983,"journal":{"name":"Jugoslovenska Medicinska Biohemija-yugoslav Medical Biochemistry","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126956926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1