Chinese Journal of Analytical Chemistry最新文献

{"title":"Bioactive compounds extracted from leaves of G. cyanocarpa using various solvents in chromatographic separation showed anti-cancer and anti-microbial potentiality in in silico approach","authors":"Mohammad Abdullah TAHER ,&nbsp;Aysha Akter LABONI ,&nbsp;Suriya Akter SHOMPA ,&nbsp;Md Mashiur RAHMAN ,&nbsp;Mohammad Mahmudul HASAN ,&nbsp;Hasin HASNAT ,&nbsp;Mala KHAN","doi":"10.1016/j.cjac.2023.100336","DOIUrl":"https://doi.org/10.1016/j.cjac.2023.100336","url":null,"abstract":"<div><p>The study was carried out to determine the possible bioactive compounds from Ethanol, Methanol, Petroleum ether, and Dichloromethane fractions of <em>Glycosmic cyanocarpa</em> (<em>G. cyanocarpa</em>). Analysis of these extracts was performed using a mass spectrometer detector installed with gas chromatography (GC–MS/MS) utilizing a method named electron impact ionization (EI). The mass spectrum of each extract was compared against the information incorporated in the library (NIST and Wiley) which provides the chemical structure with the name and molecular masses of the identified compounds. A total of 73 compounds (25 from ethanol, 19 from methanol and 5 from dichloromethane (DCM), and 24 from petroleum ether fractions) were identified from various fractions of the plants. Fourier Transform infrared spectroscopy (FTIR) analysis of the crude extracts was done to identify the functional groups of the plant derived compounds. The isolated compounds were subjected to comparison with the standard drugs towards the active binding sites of epidermal growth factor receptor (EGFR) and dihydrofolate reductase (DHFR) proteins for further evaluation of their cytotoxic and antimicrobial activity, respectively. Stigmasterol, beta-Sitosterol, Pyrazol-5-amine, 3-methyl-1,4-diphenyl-, 1,2-Benzenediol,4-(2-aminopropyl)-18,19-Secoyohimban-19-oic acid, and Phenol, 2,4-bis(1,1-dimethylethyl)-, phosphite (3:1) showed maximum binding affinity towards EGFR and 16,17,20,21-tetradehydro-16-(hydroxymethyl)-, methyl ester, (15 beta.,16<em>E</em>)-, Stigmasterol, beta-Sitosterol, Pyrazol-5-amine, 3-methyl-1,4-diphenyl- revealed highest binding affinity towards DHFR receptor. According to the current research, <em>G. cyanocarpa</em> may be a useful natural source for controlling antibacterial and anticancer activity. For thorough phytochemical screening and determining precise mechanisms of action, additional research is required.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"51 12","pages":"Article 100336"},"PeriodicalIF":1.2,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S187220402300110X/pdfft?md5=a6e8eef8679a48585e962f975bdb20ea&pid=1-s2.0-S187220402300110X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91641614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of bright blue fluorescent carbon dots and their application in highly sensitive chlorogenic acid detection","authors":"Cong-Jie PAN ,&nbsp;Xin-Xin DENG ,&nbsp;Mei-Cheng LU ,&nbsp;Xue-Zhen QIN","doi":"10.1016/j.cjac.2023.100334","DOIUrl":"https://doi.org/10.1016/j.cjac.2023.100334","url":null,"abstract":"<div><p>In this work, bright blue fluorescent carbon dots (CDs) were synthesized through a hydrothermal method by using <em>l</em>-phenylephrine as carbon source. Under exciting at 363 nm, the prepared CDs emitted bright blue fluorescence at 457 nm. The fabricated CDs showed outstanding salt tolerance, temperature stability and good resistance to photobleaching. Interestingly, the fluorescence of CDs could be distinctly quenched by chlorogenic acid (CGA). By using CDs as fluorescent probe, a novel fluorescence method for highly sensitive detection of CGA was established with a wide linear range of 0.1–220 μM and a limit of detection (LOD) as low as 33 nM. The response mechanism was also studied. The method has been successfully applied for the detection of CGA content in three samples of honeysuckle, coffee beans and Eucommia ulmoides leaves with satisfactory recoveries.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"51 12","pages":"Article 100334"},"PeriodicalIF":1.2,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204023001081/pdfft?md5=8fc45956bcb2853db4954bfdb0dd6b85&pid=1-s2.0-S1872204023001081-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90004149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study of Bazedoxifene, Exemestane, Fulvestrant, Raloxifene, Tryprostatin A, and Vorinostat compounds as potential inhibitors against breast cancer through molecular docking, and molecular dynamics simulation","authors":"Sanjeevi PANDIYAN ,&nbsp;Li WANG","doi":"10.1016/j.cjac.2023.100315","DOIUrl":"https://doi.org/10.1016/j.cjac.2023.100315","url":null,"abstract":"<div><p>Breast cancer is one of the most common cancers and topmost cause of mortality among women in both developed and developing countries. Currently available potent drugs for breast cancer exhibit adverse effects, which may be caused as a result why breast cancer-specific drugs are found to be ineffective for patients. In this study, we exploited the interaction of six potential drug compounds (Bazedoxifene, Exemestane, Fulvestrant, Raloxifene, Tryprostatin A, and Vorinostat) with three breast cancer associated proteins such as poly (ADP-ribose) polymerase-1; PARP1 (PDB ID: 5HA9) cyclin-dependent kinase 2; CDK2 (PDB ID: 6GUE), and phosphatidylinositol 3-kinases alpha; PI3Kα (PDB ID: 7K6O) using molecular docking studies. Docking results indicate that Raloxifene was shown to be the most potent inhibitor of 5HA9 protein with two hydrogen bond interactions and possesses best binding affinity of −12.3 kcal/mol. The compound Fulvestrant shows three hydrogen bond interactions and has the best binding affinity of −10.2 kcal/mol and exhibits to be the most potent inhibitor of 6GUE protein. Raloxifene indicated best binding affinity of −10.6 kcal/mol and showed to be the most potent inhibitor of 7K6O protein with two hydrogen bond interactions. Molecular dynamics simulations of 5HA9-Raloxifene, 6GUE-Fulvestrant, and 7K6O-Raloxifene were executed for 100 ns through which root mean square deviation (RMSD), root mean square fluctuation (RMSF), the number of hydrogen bonds, radius of gyration, and interaction energy was computed. The obtained results indicate that the compounds Raloxifene, and Fulvestrant can be useful for treatment of breast cancer.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"51 10","pages":"Article 100315"},"PeriodicalIF":1.2,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204023000890/pdfft?md5=6a133c76008e1ae6e280a00a5628b332&pid=1-s2.0-S1872204023000890-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90003341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urine metabolomics analysis of patients recovered of the Omicron variant of COVID-19 using ultrahigh-performance liquid chromatography with high-resolution mass spectrometry","authors":"Zhizhong TANG ,&nbsp;Yunpeng BAI ,&nbsp;Wang XU ,&nbsp;Changchun LAI ,&nbsp;Yirong WANG ,&nbsp;Yaocai LI ,&nbsp;Cuizhu HUANG ,&nbsp;Ying LI ,&nbsp;Xinyi JIANG ,&nbsp;Yingbang LI ,&nbsp;Xiyan CHEN ,&nbsp;Linhui HU ,&nbsp;Chunbo CHEN","doi":"10.1016/j.cjac.2023.100314","DOIUrl":"https://doi.org/10.1016/j.cjac.2023.100314","url":null,"abstract":"<div><h3>Background</h3><p>The novel coronavirus disease (COVID-19) outbreak has become a serious global public health crisis, particularly the newly emerged Omicron variant. Although most of the clinical symptoms of Omicron infection are mild, it spreads rapidly and the physiological changes associated with this variant are difficult to understand. This study investigates the possibility of treating renal metabolism in patients with COVID-19.</p></div><div><h3>Methods</h3><p>High-resolution mass spectrometry was used to detect non-targeted metabolomes in patients with COVID-19 (<em>n</em> = 8) and healthy subjects (<em>n</em> = 12). The main inclusion criteria were that the recovered omicron patients diagnosed as negative, met the discharge criteria and provided information consents. In this study, univariate and multivariate statistical methods were used to analyze the data between the two groups to screen different urinary metabolites associated with Omicron infection. Altered metabolic pathways related to omicron infection were also identified by examining the Kyoto Encyclopedia of Genes and Genomes database.</p></div><div><h3>Results</h3><p>The single-factor and multi-factor statistics showed that the changes in renal metabolism after treatment were significantly different from those in the control group. Approximately 3500 urinary metabolites were detected in patients recovered of the omicron variant of COVID-19 through bioinformatics methods with 296 metabolites in high confidence level. Different urinary metabolomes were also analysed to inform signal transduction pathways and prognosis prediction; some of these differential metabolites have important biological roles. Compared with the control group, the patients recovered of the omicron variant of COVID-19 exhibited dramatic changes in renal metabolism, including amino acid metabolism, ketone bodies and prolactin metabolic pathways.</p></div><div><h3>Conclusions</h3><p>Patients with the omicron variant of COVID-19 may have metabolic abnormalities in their urinary system during infection and treatment; thus, follow-up and observation should be strengthened. Thus, this study can provide a certain reference basis for further exploring the pathogenic mechanism and the metabolic indicators of COVID-19.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"51 10","pages":"Article 100314"},"PeriodicalIF":1.2,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204023000889/pdfft?md5=7f49dec850cec5b05d7fbf0e2dc05db1&pid=1-s2.0-S1872204023000889-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91639258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid method for accurate peak position extraction in high-field asymmetric ion mobility spectrometry","authors":"Jun HU ,&nbsp;Youjiang LIU ,&nbsp;Shaomin LIU ,&nbsp;Shan LI ,&nbsp;Han WANG ,&nbsp;Chilai CHEN","doi":"10.1016/j.cjac.2023.100305","DOIUrl":"10.1016/j.cjac.2023.100305","url":null,"abstract":"<div><p>High-field asymmetric waveform ion mobility spectrometry (FAIMS) enables precise identification of substances through fingerprint spectra obtained by multi-cycle scans at different separation voltages (DV) and nonlinear functions. To improve the scan speed of compensating voltage (CV) in multi-cycle scanning, the challenge arises in peak position shifts. This article proposes a method to accurately determine peak positions regardless of scan speed by exploiting the symmetric features of spectra obtained through positive and negative CV scans. The proposed method enables extremely fast scan speeds. A custom-built FAIMS system was used to verify the correlation between peak shifts and scan speed, the symmetry of spectra peaks under positive and negative CV scan modes, and the peak positions and solution errors of nonlinear functions by using benzene, styrene, acetone, toluene, m-xylene, and hydrogen sulfide as experimental samples. The results demonstrate the widespread occurrence of peak shifts, with peak deviations reaching as high as 2.49% even in slow scans of 0.75 V/s. As scan speed increases, peak position deviations gradually increase, with the maximum deviation reaching 46.83% at a scan speed of 30 V/s. By applying the proposed averaging method, peak positions of the six substances were obtained within the scan speed range of 30 to 0.75 V/s. Compared to traditional methods, the maximum peak position error using the averaging method was reduced by approximately 87.5%, and the spectrum acquisition time was reduced by 85%. The use of the averaging method reduced the calculation error of the alpha function by 88% and decreased the acquisition time by 80%. The research findings of this study offer a solution for the accurate determination of peak positions in FAIMS under fast scanning.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"51 10","pages":"Article 100305"},"PeriodicalIF":1.2,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204023000798/pdfft?md5=3f3871fb80c967afdb15eabb29548d7c&pid=1-s2.0-S1872204023000798-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80308384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of solid dispersion of Dushen decoction and establishment of its evaluation method","authors":"Feng-yuan SONG,&nbsp;Di YANG,&nbsp;Dong-yue ZHOU,&nbsp;Yu-lin DAI,&nbsp;Zi-feng PI,&nbsp;Fei ZHENG,&nbsp;Hao YUE","doi":"10.1016/j.cjac.2023.100259","DOIUrl":"https://doi.org/10.1016/j.cjac.2023.100259","url":null,"abstract":"<div><p>The preparation technology of Dushen decoction solid dispersion (SD) was determined by analyzing the preparation method, carrier type, and carrier ratio. The quality evaluation method was established by infrared analysis spectrometry and differential scanning calorimetry. By using poloxamer 188 as the carrier material and a 1:8 optimal loading ratio, the Dushen decoction SD prepared by the melting method had a better dissolution, and the preparation process was reproducible. The Dushen decoction SD was also highly uniformly dispersed in the carrier material (poloxamer 188), and the mixing mode of Dushen decoction and poloxamer 188 was physical rather than chemical. The dissolution rate of the Dushen decoction SD was nearly 16 times higher than that in the traditional dosage form of Dushen decoction. Therefore, by preparing new formulations of Dushen decoction and establishing quality evaluation methods, a scientific basis can be obtained for follow-up <em>in vivo</em> and preclinical investigation.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"51 10","pages":"Article 100259"},"PeriodicalIF":1.2,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204023000336/pdfft?md5=93d8e4934099973a8b12862f870e283d&pid=1-s2.0-S1872204023000336-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91639259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response surface methodology box-behnken design to optimise the hydrothermal synthesis of gadolinium nanoparticles","authors":"Santhy WYANTUTI ,&nbsp;Balqis FADHILATUNNISA ,&nbsp;Retna Putri FAUZIA ,&nbsp;Qi JIA ,&nbsp;Azmi Aulia RAHMANI ,&nbsp;IRKHAM ,&nbsp;Husein Hernadi BAHTI","doi":"10.1016/j.cjac.2023.100316","DOIUrl":"https://doi.org/10.1016/j.cjac.2023.100316","url":null,"abstract":"<div><p>Gadolinium (Gd) nanoparticles (NPs) are increasingly considered as a viable alternative to clinically employed Gd chelates in magnetic resonance imaging (MRI). The utilisation of these materials as contrast agents offers several advantages including lower toxicity, prolonged circulation time, and a sufficiently high Gd content, thereby enhancing disease imaging during MRI diagnosis. Therefore, this study synthesised Gd NPs using the hydrothermal method based on the response surface methodology Box-Behnken design (RSM-BBD) to determine the optimal conditions. In this experimental design, three independent variables, the mass of Gd₂O₃ (g), the synthesis temperature (°C) and time (h), were optimised to obtain sufficiently sized nanoparticles for further biomedical applications. In addition, polyethene glycol-6000 (PEG-6000) was used as a stabiliser to form uniformly sized nanoparticles. The optimal conditions were 0.4910 g of Gd₂O₃, a temperature of 180 °C, and a synthesis time of 7 h. Characterisation by scanning electron microscope-energy dispersive X-ray (SEM-EDX) and transmission electron microscope (TEM) demonstrated that the Gd NPs were spherical with a size range below 20 nm. Fourier transform infrared (FTIR) spectroscopy identified PEG molecules with low intensity on the Gd NPs and the obtained zeta potential value was +36.7±0.802 mV. The RSM-BBD analysis applied in this study facilitated the determination of the optimal synthesis conditions.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"51 10","pages":"Article 100316"},"PeriodicalIF":1.2,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204023000907/pdfft?md5=1bc12939589282fc65bf74b22fd71bf7&pid=1-s2.0-S1872204023000907-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90003343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A near-infrared phototheranostic probe for in vivo hyaluronidase detection and activatable imaging-guided synergistic therapy of triple negative breast cancer","authors":"Lihong LI ,&nbsp;Rongrong HU ,&nbsp;Xinyu ZHANG ,&nbsp;Jiaojiao LI ,&nbsp;Lei DING ,&nbsp;Wen LIU ,&nbsp;Haojiang WANG ,&nbsp;Bin WANG ,&nbsp;Chengwu ZHANG ,&nbsp;Lili YAN ,&nbsp;Lixia GUO ,&nbsp;Sufang MA ,&nbsp;Haipeng DIAO","doi":"10.1016/j.cjac.2023.100300","DOIUrl":"10.1016/j.cjac.2023.100300","url":null,"abstract":"<div><p>Activatable phototheranostic probes may become novel option for tumor diagnosis and treatment due to their abilities to accurately detect cancer and guide therapeutic intervention through imaging. In this study, a hyaluronidase (HAase)-responsive near-infrared phototheranostic probe (HA-ICG-PDA) has been developed by covalently coating indocyanine green (ICG)-decorated hyaluronic acid (HA) onto the surface of polydopamine (PDA) for the imaging and therapy of triple negative breast cancer (TNBC). The probe exhibits sensitive and selective fluorescence enhancement to HAase, enabling the detection of HAase and activatable near-infrared fluorescence imaging of HAase-overexpressed tumor cells. Moreover, the combination of ICG and PDA equips HA-ICG-PDA with outstanding photodynamic and enhanced photothermal effects upon 808 nm laser irradiation. Both in vitro and in vivo experiments have confirmed that HA-ICG-PDA is capable of realizing imaging diagnosis of TNBC through HAase-activated fluorescence signal. With the guidance of fluorescence imaging, the probe displays synergistic photothermal and photodynamic therapy effects against TNBC, with minimal damage to the normal tissues. Therefore, the proposed phototheranostic probe paves a new avenue for HAase detection, and subsequently, diagnosis and imaging-guided treatment of TNBC.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"51 9","pages":"Article 100300"},"PeriodicalIF":1.2,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204023000749/pdfft?md5=5ef316459467140a9f7e009ae8bb9763&pid=1-s2.0-S1872204023000749-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74548211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical characterization, multivariate analysis and in vitro bioactivity evaluation of the roots of Fraxinus mandshurica","authors":"Jianjin GUO ,&nbsp;Lu BAI ,&nbsp;Chi-Tang HO ,&nbsp;Sen GUO ,&nbsp;Naisheng BAI","doi":"10.1016/j.cjac.2023.100303","DOIUrl":"10.1016/j.cjac.2023.100303","url":null,"abstract":"<div><p><em>Fraxinus mandshurica</em> is widely used in folk medicine to treat various diseases and has significant pharmacological effects. To make full use of this resource, its roots were studied. Twelve lignans were first obtained by natural product extraction, selective separation and identification, and were identified and quantified by high performance liquid chromatography-photodiode array (HPLC-DAD). The results of quantitative analysis showed that the highest lignin content in <em>F. mandshurica</em> roots was compound <strong>9</strong> with 102.02 mg/g, while the overall lignin content in Shanxi was low. The results of methodological validation showed that the RSD values of intra-day precision ranged from 0.35% to 1.38%, while the RSD values of inter-day precision were within 1.97%; the RSD values of reproducibility experiments ranged from 0.35% to 1.96%, while the RSD values of stability experiments were less than 2.97%; the spiked recovey of samples were within 102.84%, and the RSD values ranged from 0.39% to 1.77%, which fully confirmed the high precision and good reproducibility of the analytical method. In addition, fingerprinting and heatmap were established for 40 batches of samples from 8 origins. Next, multivariate analysis, including principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA), was performed to investigate the differences in lignin content in the roots of <em>F. mandshurica</em> from different origins, and four lignins that could be used to distinguish <em>F. mandshurica</em> from different origins were screened. Finally, 12 lignans were evaluated for enzyme activity inhibition with α-glucosidase, tyrosinase and acetylcholinesterase, and the results showed that all 12 lignans exhibited enzyme activity inhibition to different degrees, which have potential applications in anti-diabetic, whitening and light spot and anti-Alzheimer's disease, and become a new source of natural enzyme activity inhibitors. This study established an identification and evaluation method applicable to plants of different origins, which provides a certain theoretical basis for the comprehensive exploitation of <em>F. mandshurica</em>.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"51 9","pages":"Article 100303"},"PeriodicalIF":1.2,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204023000774/pdfft?md5=12ad8c25a512647518469d569e215a8f&pid=1-s2.0-S1872204023000774-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73693479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enrichment, analysis, identification and mechanism of antioxidant components in Toona sinensis","authors":"Yunxiao Wang ,&nbsp;Dongyu Gu ,&nbsp;Chang Liu ,&nbsp;Shanshan Tang ,&nbsp;Shuai Wang ,&nbsp;Yi Wang ,&nbsp;Yi Yang","doi":"10.1016/j.cjac.2022.100198","DOIUrl":"https://doi.org/10.1016/j.cjac.2022.100198","url":null,"abstract":"<div><p>The tender leaves of <em>Toona sinensis</em> not only taste delicious, but also have a wide range of biological activities, including strong antioxidant activity. In this study, a method based on liquid-liquid-refining extraction and bioassay-guided separation were established and developed for enrichment, analysis and identification of antioxidant components in <em>T. sinensis</em>. Two main antioxidants, 1,2,3,4,6-penta-O-galloyl-β-D-glucose (PGG) and ethyl gallate (EG) with strong antioxidant capacity were responsible for the antioxidant activity of <em>T. sinensis</em>. The EC₅₀ of EG scavenging ABTS·⁺ and DPPH· were 22.53 ± 0.25 µM and 38.43 ± 0.66 µM, and the EC₅₀ of PGG were 13.72 ± 0.17 µM and 17.33 ± 0.21 µM. The thermodynamic parameters of various pathways indicated that single electron transfer followed by proton transfer (SET-PT) was the main antioxidant mechanism of PGG and EG. Furthermore, the results of frontier molecular orbital theory and reaction kinetics showed that PGG had low energy gap and reaction energy barrier, which was the fundamental reason why its antioxidant activity was better than EG.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"51 9","pages":"Article 100198"},"PeriodicalIF":1.2,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"1874954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}