Chinese Journal of Analytical Chemistry最新文献

{"title":"Chemical characterization, multivariate analysis and in vitro bioactivity evaluation of the roots of Fraxinus mandshurica","authors":"Jianjin GUO ,&nbsp;Lu BAI ,&nbsp;Chi-Tang HO ,&nbsp;Sen GUO ,&nbsp;Naisheng BAI","doi":"10.1016/j.cjac.2023.100303","DOIUrl":"10.1016/j.cjac.2023.100303","url":null,"abstract":"<div><p><em>Fraxinus mandshurica</em> is widely used in folk medicine to treat various diseases and has significant pharmacological effects. To make full use of this resource, its roots were studied. Twelve lignans were first obtained by natural product extraction, selective separation and identification, and were identified and quantified by high performance liquid chromatography-photodiode array (HPLC-DAD). The results of quantitative analysis showed that the highest lignin content in <em>F. mandshurica</em> roots was compound <strong>9</strong> with 102.02 mg/g, while the overall lignin content in Shanxi was low. The results of methodological validation showed that the RSD values of intra-day precision ranged from 0.35% to 1.38%, while the RSD values of inter-day precision were within 1.97%; the RSD values of reproducibility experiments ranged from 0.35% to 1.96%, while the RSD values of stability experiments were less than 2.97%; the spiked recovey of samples were within 102.84%, and the RSD values ranged from 0.39% to 1.77%, which fully confirmed the high precision and good reproducibility of the analytical method. In addition, fingerprinting and heatmap were established for 40 batches of samples from 8 origins. Next, multivariate analysis, including principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA), was performed to investigate the differences in lignin content in the roots of <em>F. mandshurica</em> from different origins, and four lignins that could be used to distinguish <em>F. mandshurica</em> from different origins were screened. Finally, 12 lignans were evaluated for enzyme activity inhibition with α-glucosidase, tyrosinase and acetylcholinesterase, and the results showed that all 12 lignans exhibited enzyme activity inhibition to different degrees, which have potential applications in anti-diabetic, whitening and light spot and anti-Alzheimer's disease, and become a new source of natural enzyme activity inhibitors. This study established an identification and evaluation method applicable to plants of different origins, which provides a certain theoretical basis for the comprehensive exploitation of <em>F. mandshurica</em>.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"51 9","pages":"Article 100303"},"PeriodicalIF":1.2,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204023000774/pdfft?md5=12ad8c25a512647518469d569e215a8f&pid=1-s2.0-S1872204023000774-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73693479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enrichment, analysis, identification and mechanism of antioxidant components in Toona sinensis","authors":"Yunxiao Wang ,&nbsp;Dongyu Gu ,&nbsp;Chang Liu ,&nbsp;Shanshan Tang ,&nbsp;Shuai Wang ,&nbsp;Yi Wang ,&nbsp;Yi Yang","doi":"10.1016/j.cjac.2022.100198","DOIUrl":"https://doi.org/10.1016/j.cjac.2022.100198","url":null,"abstract":"<div><p>The tender leaves of <em>Toona sinensis</em> not only taste delicious, but also have a wide range of biological activities, including strong antioxidant activity. In this study, a method based on liquid-liquid-refining extraction and bioassay-guided separation were established and developed for enrichment, analysis and identification of antioxidant components in <em>T. sinensis</em>. Two main antioxidants, 1,2,3,4,6-penta-O-galloyl-β-D-glucose (PGG) and ethyl gallate (EG) with strong antioxidant capacity were responsible for the antioxidant activity of <em>T. sinensis</em>. The EC₅₀ of EG scavenging ABTS·⁺ and DPPH· were 22.53 ± 0.25 µM and 38.43 ± 0.66 µM, and the EC₅₀ of PGG were 13.72 ± 0.17 µM and 17.33 ± 0.21 µM. The thermodynamic parameters of various pathways indicated that single electron transfer followed by proton transfer (SET-PT) was the main antioxidant mechanism of PGG and EG. Furthermore, the results of frontier molecular orbital theory and reaction kinetics showed that PGG had low energy gap and reaction energy barrier, which was the fundamental reason why its antioxidant activity was better than EG.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"51 9","pages":"Article 100198"},"PeriodicalIF":1.2,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"1874954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the plasma metabolomics of Schisandra chinensis polysaccharide against ulcerative colitis and its correlation with gut microbes metabolism","authors":"Guangji ZHANG ,&nbsp;Jiuba ZHANG ,&nbsp;Shuai YAN ,&nbsp;Min HAO ,&nbsp;Chenghao FEI ,&nbsp;De JI ,&nbsp;Chunqin MAO ,&nbsp;Huangjin TONG ,&nbsp;Tulin LU ,&nbsp;Lianlin SU","doi":"10.1016/j.cjac.2023.100244","DOIUrl":"https://doi.org/10.1016/j.cjac.2023.100244","url":null,"abstract":"<div><p>In our previous study, the efficacy of <em>Schisandra chinensis</em> polysaccharide (SCP) against ulcerative colitis (UC) has been evaluated. At the same time, the mechanism of action was studied from the structure and composition of intestinal flora and the metabolism of short chain fatty acids (SCFAs). Metabolomics method was used to study the mechanism of SCP against UC which needed further analysis, and research results were carried out to conduct correlation analysis in order to further and systematically reveal the mechanism of SCP in the intervention of UC based on previous studies. In the study of metabolomics, 32 potential biomarkers including Sphinganine, PG (18:0/16:0), PE (P-16:0/16:0), LysoPC (18:1(9Z)/0:0), LysoPE (0:0/18:4(6Z,9Z,12Z,15Z)), Vitamin D2 3-glucuronide, etc. were identified in plasma, which were assigned to glycerol phospholipid metabolism, sphingolipid metabolism, ether lipid metabolism, and linoleic acid metabolism. These pathways are associated with diseases such as intestinal dysfunction, inflammatory syndromes, and neurological dysfunction. In addition, SCP was able to significantly improve the imbalance of gut microbiota, which was closely related to SCFAs. Based on the above research, we can draw a conclusion that SCP has pharmacological effects on UC, and its mechanism may be related to the gut microbes, plasma biomarkers and short-chain fatty acids.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"51 9","pages":"Article 100244"},"PeriodicalIF":1.2,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"1894803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorescence and surface enhanced Raman scattering dual-model PCR for the detection of DNA barcoding of Asarum heterotropoides","authors":"Chunhao WEI,&nbsp;Aihong WU,&nbsp;Lingling GUO,&nbsp;Liqiang LIU,&nbsp;Chuanlai XU,&nbsp;Hua KUANG,&nbsp;Xinxin XU","doi":"10.1016/j.cjac.2023.100304","DOIUrl":"10.1016/j.cjac.2023.100304","url":null,"abstract":"<div><p>Asarum plants containing many functional substances are widely used in traditional prescriptions and tonics. DNA barcoding is a relatively short specific gene segment in the genome which can be used for species identification purposes. The present work selected RNA transcriber type II intron shear enzyme gene (matK) as the target and developed a novel fluorescence and surface enhanced Raman scattering (SERS) dual-model PCR for the detection of <em>Asarum heterotropoides</em> (AH) in plants. The dual-model method achieved specific AH DNA detection with a limit of detection (LOD) of 10⁻³ ng/µL for the fluorescence signal and 10⁻⁴ ng/µL for the SERS signal in the range of 10⁻⁴−10 ng/µL. Agarose gel electrophoresis results and qPCR results showed the novel method was highly specific for AH detection and only the AH was successfully amplified.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"51 9","pages":"Article 100304"},"PeriodicalIF":1.2,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204023000786/pdfft?md5=f75c2129821405a2c0004102d1aa101f&pid=1-s2.0-S1872204023000786-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83850557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and application of an underwater mass spectrometer for in situ detection of deep-sea dissolved gases","authors":"Han WANG ,&nbsp;Lei SHAO ,&nbsp;Chenguang YANG ,&nbsp;Jian ZHANG ,&nbsp;Changjie LIU ,&nbsp;Zhangxu WU ,&nbsp;Haiyun SONG ,&nbsp;Chilai CHEN","doi":"10.1016/j.cjac.2023.100299","DOIUrl":"https://doi.org/10.1016/j.cjac.2023.100299","url":null,"abstract":"<div><p>The rapid and accurate analysis of dissolved gases in the deep sea has great significance for studying the geological profile of the Earth, searching for oil and gas resources, and studying global climate change. In this research, we developed a deep-sea mass spectrometer for in situ dissolved gases detection. The deep-sea mass spectrometer uses 24 V DC power and has an average power consumption of less than 60 W during operation and less than 10 W during standby. Its physical size is <em>φ</em>240 mm×1300 mm, with a core mass of 14.2 kg. The detectable mass range is 1–200 Da, with a mass resolution of less than 1 Da and the scanning time for a single mass is less than 15 ms. The detection limits for dissolved N₂, O₂, Ar, and CO₂ under atmospheric pressure are 0.021 μg/L, 0.068 μg/L, 0.017 μg/L, and 0.014 μg/L. The deep-sea mass spectrometer operated continuously and stably for more than 8 h under a simulated water depth of 5800 m, and it also achieved continuous online detection for 25.8 h in the sea area of −1388 m depth in the South China Sea, obtaining dissolved gas concentration-time and depth-concentration curves. This research provides key technological support for deep-sea exploration and information reference for other deep-sea detection technologies.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"51 9","pages":"Article 100299"},"PeriodicalIF":1.2,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"1874953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-target screening and identification of the significant quality markers in the wild and cultivated Cordyceps sinensis using OPLS-DA and feature-based molecular networking","authors":"Jing WANG ,&nbsp;Qinyu XIAO ,&nbsp;Hongbo HUANG ,&nbsp;Dan WU ,&nbsp;Guangfeng ZENG ,&nbsp;Wenrui CHEN ,&nbsp;Yiwen TAO ,&nbsp;Bo DING","doi":"10.1016/j.cjac.2023.100302","DOIUrl":"10.1016/j.cjac.2023.100302","url":null,"abstract":"<div><p><em>Cordyceps sinensis</em> is a rare traditional Chinese herbal material. The cultivated <em>cordyceps sinensis</em> instead of the wild is a potential important development trend. Assessing the consistency of the wild and cultivated <em>cordyceps sinensis</em> is a very significant attention. The method of non-target screening and mining of the significant quality markers in the wild and cultivated <em>cordyceps sinensis</em> samples was established by liquid chromatography-quadrupole time-of-flight high resolution mass spectrometry (LC-Q-TOF-MS) combined with feature-based molecular networking (FBMN) and the model of orthogonal partial least square discriminant analysis model (OPLS-DA). Forty-seven training samples, including thirty-three wild <em>cordyceps sinensis</em> samples and fourteen cultivated <em>cordyceps sinensis</em> samples, were firstly used to build the OPLS-DA original model based on the 6827 feature <em>m/z</em> peaks. The 1144 feature <em>m/z</em> peaks of dimensionality reduction were built the OPLS-DA optimized model, which were acquired by the variable projected importance (VIP) of the OPLS-DA original model. Twenty nine significant markers were mined by using the S-plot of the OPLS-DA optimized model. Moreover, 17 of 29 significant markers were identified by the non-target screening of FBMN, including eight wild markers and nine cultivated markers. Finally, an overall correct rate of 95.5% (twenty two test samples) was obtained for classification of the wild and cultivated <em>cordyceps sinensis</em> samples based on the twenty nine significant markers. It is indicated that the significant quality markers of <em>cordyceps sinensis</em> could be mined and identified based on the non-target screening of FBMN coupled with OPLS-DA.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"51 9","pages":"Article 100302"},"PeriodicalIF":1.2,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204023000762/pdfft?md5=434fb0d0484c86543923679f83f5ff9d&pid=1-s2.0-S1872204023000762-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91074050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and identification of active compounds of GanZhiRong granule based on liquid chromatography-mass spectrometry and biomolecular networks","authors":"Si-Tong CHEN ,&nbsp;Zhuang XIONG ,&nbsp;Chenxu JING ,&nbsp;Ran XIA ,&nbsp;Xue QU ,&nbsp;Tie-Jun LIU ,&nbsp;Yang-Yang LIU","doi":"10.1016/j.cjac.2023.100294","DOIUrl":"https://doi.org/10.1016/j.cjac.2023.100294","url":null,"abstract":"<div><h3>Objective</h3><p>To investigate the mechanism of GanZhiRong granule(GZR)in the treatment of type 2 diabetes mellitus (T2DM).</p></div><div><h3>Methods</h3><p>Network pharmacology was used to explore the target and signal pathway of GZR in T2DM. Cell experiments were conducted to observe the inhibitory effects of GZR on oxaloacetic acid on pyruvate glycoisogenesis, triglyceride and glucose release, and the inhibitory effects of GZR on palmitic acid (PA) induced apoptosis of HepG2 cells. The main components in GZR were identified and compared by liquid chromatography-mass spectrometry.</p></div><div><h3>Results</h3><p>Network pharmacological methods were used to explore the targets and signaling pathways of T2DM treated with GZR, and it was found that CA4, CA7, CA2, AKR1B1 and other targets were closely related to T2DM treated with GZR. Gene Ontology (GO) and Kyoto Encyclopedia Genes and Genomes (KEGG) pathway enrichment analysis indicated that GZR may directly regulate the development of T2DM through lipid atherosclerosis and apoptosis. <em>In vitro</em> results showed that GZR has a certain protective effect on PA-induced damage of HepG2 cells in a dose-dependent manner. In addition, GZR can effectively inhibit the increase of triglyceride and glucose contents in PA-induced HepG2 cells, decrease the contents of pyruvate and oxaloacetic acid, and significantly increase the content of acetyl-coA. The main components were identified and compared. 32 components were identified, including salvianolic acid B, D-hydrothreose, proanthocyanidin, shikimic acid, salvianolic acid A, salvianolic acid C, 7 glycosides of calycoflavone, 3′-methoxy puerarin, 3′-hydroxypuerarin, apigenin-7-o-β-D.</p></div><div><h3>Conclusion</h3><p>The treatment of T2DM with GZR is related to lipid metabolism and cell apoptosis.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"51 9","pages":"Article 100294"},"PeriodicalIF":1.2,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3338877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-time detection of the transport of water cluster across cytomembrane with piezoelectric sensor","authors":"Peihui Yang,&nbsp;Yeyan Zhu,&nbsp;Yan Hao","doi":"10.1016/j.cjac.2023.100307","DOIUrl":"https://doi.org/10.1016/j.cjac.2023.100307","url":null,"abstract":"<div><p>Acquiring insight into the transport of water clusters across cytomembrane is vital and essential for elucidating cell functions and water channels. Herein, A highly sensitive living cell analysis platform was developed for monitoring the transport dynamics of water clusters through water channels by combining the piezoelectric transducer and the hypotonicity-caused cell volume regulation. The various drinking water was used as the hypotonic perfusate to stimulate vascular endothelial cells and the temporal frequency response to cells was obtained to analyze the transport rate of the influx and efflux of water clusters which is related to water structure. The sensor is capable to distinguish the transport dynamics of different water clusters in five drinking water which the transport rates of water influx of cells are 5.1 ± 0.40, 6.6 ± 0.96, 7.4 ± 0.46, 8.1 ± 0.86, and 8.4 ± 0.80 Hz min⁻¹ (<em>n</em> = 3), respectively, showed that they are ranked in the increasing order: M-water, C-water, S-water, RO-water, <span>d</span>-water. Moreover, inhibitor of HgCl₂ was used to block water channels to reveal the water transport via water channels. Conductivity and infrared spectroscopy assays further confirmed the differences of transport dynamics between these drinking water due to the structure or size of water clusters caused by the mineral substance or the electromagnetic field. Therefore, this work provides a novel, simple and effective method for monitoring the transport of different water cluster across cytomembrane.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"51 12","pages":"Article 100307"},"PeriodicalIF":1.2,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71725984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly selective detection of formaldehyde and its analogs in clams based on unique and specific conjugation reactions via ultraviolet-visible absorptions","authors":"Yuru Min ,&nbsp;Donglei Fu ,&nbsp;Honglei Liu ,&nbsp;Chenyao Yuan ,&nbsp;Lei Wang ,&nbsp;Mengyu Cao ,&nbsp;Aowei Sun ,&nbsp;Wenrong Yang ,&nbsp;Jingquan Liu","doi":"10.1016/j.cjac.2023.100287","DOIUrl":"https://doi.org/10.1016/j.cjac.2023.100287","url":null,"abstract":"<div><p>Although formaldehyde has been widely used in industry, the excessive or residual formaldehyde has been proven to cause irreversible harm to human beings due to its high toxicity. To solve this problem, we studied oxalyldihydrazide as an analytical reagent to determine formaldehyde by ultraviolet and visible (UV-vis) spectrophotometry. In this study, hydrazine compounds with –NH–NH₂ structures can react with formaldehyde to form hydrazone (including –NH–N=CH–), water is the only by-product. Therefore, oxalyldihydrazide can not only react with formaldehyde but also sense its analogs such as acetaldehyde, acetone, and benzaldehyde in solution, and detect them by UV-vis spectrophotometry at 220 nm, 226 nm, 262 nm, and 257 nm respectively. Among them, the limit of detection (LOD) of formaldehyde is calculated to be 0.03 ppm (LOQ = 0.03 ppm) with the relative standard deviation (RSD) of 1.58%. Meanwhile, the above-mentioned method can be used for detecting formaldehyde in clams with excellent selectivity and sensitivity.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"51 8","pages":"Article 100287"},"PeriodicalIF":1.2,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"1553843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of unsaturated lipids with high structural specificity by coupling epoxidation with tandem mass spectrometry","authors":"Wenbo CAO,&nbsp;Jun TAN","doi":"10.1016/j.cjac.2023.100290","DOIUrl":"https://doi.org/10.1016/j.cjac.2023.100290","url":null,"abstract":"<div><p>Comprehensive characterization of lipid structures is of significance, as they play a key role in numerous biological processes. Mass spectrometry (MS) emerges as a preferred tool for lipid analysis, providing good performance in sensitivity and accuracy. However, characterization of unsaturated lipids still has certain difficulties due to structural diversity. In this work, combining epoxidation and tandem MS was developed for unsaturated lipids analysis with high structural specificity. By using the proposed method, it could simultaneously produce high-abundance diagnostic ions specific to lipid C=C locations and <em>sn</em>-positions, facilitating lipid isomers identification. Determining C=C location and <em>sn</em>-position isomers of lipid in a single spectrum can be achieved, which is valuable for discovery of great structural diversity. The capability of this approach in characterizing unsaturated lipids at high structural level has been illustrated with different standards including a lipid extract from complex biological sample. The relative quantitation capability of C=C location and <em>sn</em>-position isomers was also demonstrated based on diagnostic ion intensities.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"51 8","pages":"Article 100290"},"PeriodicalIF":1.2,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"1875766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}