Chinese Journal of Analytical Chemistry最新文献

{"title":"Heavy metals concentration and human health risk assessment in tobacco cigarette products from Tanzania","authors":"Amos Vincent NTARISA","doi":"10.1016/j.cjac.2024.100428","DOIUrl":"10.1016/j.cjac.2024.100428","url":null,"abstract":"<div><p>Cigarette smoke contains various harmful substances, including heavy metals, that pose significant health risks. Tobacco smoking is the leading cause of lung cancer, accounting for approximately 90 % of lung cancer-related deaths worldwide. However, limited information is available on the health hazards posed by heavy metals in cigarettes commonly consumed in Tanzania. This study aims to assess the potential health risks associated with heavy metal exposure from eight most commonly consumed cigarette products in Tanzania using atomic absorption spectrometry. The concentrations (mg kg⁻¹) of copper (Cu), zinc (Zn), cadmium (Cd), chromium (Cr), and nickel (Ni) ranged from 6.94 to 16.31, 0.6–1.93, 0.4–0.66, 0.69–2.86, and 0.11–2.69, respectively, with mean concentrations of 9.35, 0.92, 0.52, 1.82, and 1.24 mg kg⁻¹. The mean concentrations of Cu and Zn are within the permissible limits set by FAO/WHO while Cd, Cr, and Ni exceeded these limits, raising concerns. A high positive correlation is observed between Cu and Zn (<span><math><mrow><mi>r</mi><mo>=</mo><mn>0.988</mn></mrow></math></span>, <span><math><mrow><mi>p</mi><mo>=</mo><mn>0.01</mn></mrow></math></span>). The non-carcinogenic hazard quotient (HQ) for ingestion is below 1, indicating minimal risk, whereas HQ for inhalation is above 1, indicating considerable health risk. The carcinogenic risk assessment for ingestion is within acceptable limits for all metals. However, Cd, Cr, and Ni present higher risks than the acceptable limit set by USEPA (1E-06 to 1E-04), suggesting significant cancer risk associated with these metals. This study highlights the need for stringent regulation and monitoring of heavy metal content in cigarettes to mitigate health risks.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 8","pages":"Article 100428"},"PeriodicalIF":1.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204024000732/pdfft?md5=95879f6d5c96a12a44fcd53e7f751f61&pid=1-s2.0-S1872204024000732-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142058534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solid phase extraction coupled with gas chromatography-triple quadrupole mass spectrometry for the rapid and simultaneous determination of forty-nine parent and halogenated polycyclic aromatic hydrocarbons in human serum","authors":"Jiali HAN ,&nbsp;Yaxuan WANG ,&nbsp;Jun JIN ,&nbsp;Jicheng HU","doi":"10.1016/j.cjac.2024.100421","DOIUrl":"10.1016/j.cjac.2024.100421","url":null,"abstract":"<div><p>Halogenated polycyclic aromatic hydrocarbons (H-PAHs) are the emerging organic pollutants in environment, which are ubiquitous in the atmosphere, soil and water. Some H-PAH congeners have been proven to have dioxin-like toxicity. However, the research on the human internal exposure of H-PAHs is still limited. A simple and efficient analytical method is essential for the study of human internal exposure, so a method for the rapid and simultaneous determination of 16 polycyclic aromatic hydrocarbons (PAHs) and 33 H-PAHs in human serum was established in this study. After 1.0 mL serum was extracted with ethyl acetate/acetonitrile (1:4) solution containing 1 % formic acid, samples were purified by Captiva EMR-Lipid solid phase extraction column, and then determined by gas chromatography tandem triple quadrupole mass spectrometer (GC-MS/MS). The results showed that the recoveries of PAHs and H-PAHs ranged from 71.2 % to 119 % and 68.8 % to 121 %, respectively. When 1.0 mL of serum sample is used, the method detection limit of PAHs and H-PAHs is 0.001–0.314 ng/mL. The method is simple and efficient, and suitable for the simultaneous determination of trace PAHs and H-PAHs in human serum. High concentrations of chlorinated polycyclic aromatic hydrocarbons were detected in pooled serum, of which 1,5-dichloranthracene was the main contributor congener, with a concentration of 204–328 ng/g lipid, which deserved more attention.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 8","pages":"Article 100421"},"PeriodicalIF":1.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204024000665/pdfft?md5=a96efab8bda5bd6f11963f483a7635ea&pid=1-s2.0-S1872204024000665-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141838517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic particle size study of immunogenic complexes of PF4/heparin based on photon correlation spectroscopy","authors":"He TIAN ,&nbsp;Yuyao WEI ,&nbsp;Yu HE ,&nbsp;Jingwen ZHAO ,&nbsp;Yilan OUYANG ,&nbsp;Haiying LIU ,&nbsp;Lin YI ,&nbsp;Zhenqing ZHANG","doi":"10.1016/j.cjac.2024.100419","DOIUrl":"10.1016/j.cjac.2024.100419","url":null,"abstract":"<div><p>In this work, the process of forming PF4/heparin complex was described based on their sizes measured with PCS. PF4/heparin complexes formed when a small amount of heparin was added to PF4, and they grew to bigger particles when more heparin was added, but they started to decompose after a certain amount of heparin was added. The larger the molecular weight of heparin has, the less amount of heparin is required to form the complex with PF4. This process was verified by the results of the complex surface charge. This method was used to compare the immunogenicity among the different batches of nadroparin after its specificity and stability were validated.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 8","pages":"Article 100419"},"PeriodicalIF":1.2,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204024000641/pdfft?md5=b33de89adc61dd75e2fba6565d7e882f&pid=1-s2.0-S1872204024000641-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141848349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasensitive tadalafil detection with eco-friendly CeO2/Al-pillared clay incorporated pencil graphite paste electrode","authors":"Mohamed IBRAHIM,&nbsp;Yassien TEMERK,&nbsp;Hossieny IBRAHIM,&nbsp;Mohamed SALAH","doi":"10.1016/j.cjac.2024.100418","DOIUrl":"10.1016/j.cjac.2024.100418","url":null,"abstract":"<div><p>In this study, we have constructed a novel voltammetric sensor based on montmorillonite clay (MMT) incorporated with CeO₂ nanoparticles using a composite graphite paste electrode as a cross linker (MMT-CeO₂NPs/GPG-PE) for the trace determination of tadalafil (TAD) drug. The characterization of CeO₂ nanoparticles has been conducted using various analytical techniques, including X-ray diffraction, transmission electron microscopy, and high-resolution transmission electron microscopy. The morphology of the composite of CPG and MMT-CeO₂NPs/CPG was elucidated through scanning electron microscopy (SEM). The newly developed sensor (MMT-CeO₂NPs/CPG-PE) exhibited remarkable efficiency towards TAD oxidation using adsorptive stripping square-wave voltammetry (AdS-SWV) in Mcllvaine buffer solution (pH 8.0). A highly selective and sensitive method for TAD detection has been successfully applied based on MMT-CeO₂NPs/CPG-PE, showing two different linear concentration ranges of 0.005−0.1 and 0.1–9.9 µM. The LOD and LOQ were determined to be 1.97 × 10⁻¹⁰ and 6.57 × 10⁻¹⁰ M, respectively, with a sensitivity of 3916 µA µM⁻¹ cm⁻². Interestingly, the sensing electrode exhibited excellent reproducibility, reusability, and stability even after 30 days. Moreover, the newly developed nano-sensor (MMT-CeO₂NPs/CPG-PE) has been effectively utilized for the accurate detection of TAD in pharmaceutical formulations and spiked human blood serum and urine samples, demonstrating no interference from other substances.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 7","pages":"Article 100418"},"PeriodicalIF":1.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S187220402400063X/pdfft?md5=e0a8a045be6e6c69477b4891b4a51d35&pid=1-s2.0-S187220402400063X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141841383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radiosensitizer-based injectable hydrogel for enhanced radio-chemotherapy of TNBC","authors":"Jie AN ,&nbsp;Kaile CHU ,&nbsp;Qin ZHOU ,&nbsp;Huizhu MA ,&nbsp;Qianwen HE ,&nbsp;YaQiong ZHANG ,&nbsp;Junping LV ,&nbsp;Hua WEI ,&nbsp;Min LI ,&nbsp;Zhifang WU ,&nbsp;Sijin LI","doi":"10.1016/j.cjac.2024.100414","DOIUrl":"https://doi.org/10.1016/j.cjac.2024.100414","url":null,"abstract":"<div><p>Radionuclide therapy (RNT) stands out as a highly effective method for treating solid tumors. However, its therapeutic efficiency faces challenges due to the radioresistance of tumors, the limited penetration depth and intracellular deposition of rays in tumor tissue, which causes residue of living cancer cells. Herein, we report a novel approach by utilizing radionuclide ¹³¹I-labelled polydopamine encapsulated gold nanoparticle co-loaded with the classical anticancer drug gemcitabine within a hydrogel formed from oxidized glucan and chitosan hydrochloride, combining RNT with chemotherapy for cancer treatment. Au, as a high Z element, is able to interact with short-range β-rays to emit bremsstrahlung and secondary charged particles which in turn increase the dose deposited in tumor cells. Simultaneously, gemcitabine is able to affect cell cycle redistribution, resulting in an increase in the radiosensitive cellular component of the cycle, and gemcitabine also inhibits the repair of radioactive damage to cellular DNA, which has a radiosensitizing effect. In both <em>in vivo</em> and <em>vitro</em> experiments, the injectable hydrogel demonstrates excellent biosecurity, stability in radionuclide labeling, and capabilities for single-photon emission computed tomography (SPECT) imaging. Compared analysis with single RNT revealed that combination therapy markedly inhibits the growth of triple-negative breast cancer. This integrated therapeutic strategy establishes an efficient tumor synergistic treatment platform, offering new avenues for advancing radionuclide therapy in the clinical treatment of cancer.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 7","pages":"Article 100414"},"PeriodicalIF":1.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204024000598/pdfft?md5=efa5603318eb02718afa29f9a82e2704&pid=1-s2.0-S1872204024000598-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141596081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An ultrasensitive strip sensor for rapid detection of African swine fever virus","authors":"Mengjing ZHANG,&nbsp;Lingling GUO,&nbsp;Xinxin XU,&nbsp;Hua KUANG,&nbsp;Chuanlai XU,&nbsp;Liqiang LIU","doi":"10.1016/j.cjac.2024.100416","DOIUrl":"10.1016/j.cjac.2024.100416","url":null,"abstract":"<div><p>African swine fever (ASF) is a highly contagious disease caused by the African swine fever virus (ASFV) infecting pigs, which has caused huge economic losses in countries around the world. Currently, there is no effective vaccine, and the prevention and control of ASF is mainly through rapid detection, so it is particularly important to carry identify and develop rapid detection methods for ASFV. In this study, recombinant plasmid PET-28a(+)-p30 was constructed, and the recombinant protein was obtained by inducing expression and Ni⁺ resin affinity column purification. Mice were immunized with recombinant p30 protein, and after three immunizations, ten strains of hybridoma cells that stably secreted monoclonal antibodies (mAbs) against p30 protein were obtained by cell fusion and subcloning. A colloidal gold immunochromatography assay (GICA) based on double antibody sandwich technology was established to screen the paired antibodies, and the trapping and detecting antibodies were mAb-11F11 and mAb-7A8, respectively, with a detection limit of 1 ng/mL, which laid an important material foundation for the early detection of ASF in the future.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 7","pages":"Article 100416"},"PeriodicalIF":1.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204024000616/pdfft?md5=8fed3b942e38d115d709b37bdc5d58a6&pid=1-s2.0-S1872204024000616-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141844461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"State of the Art: Natural fibre-reinforced composites in advanced development and their physical/chemical/mechanical properties","authors":"Krishnasamy KARTHIK ,&nbsp;Ramesh kumar RAJAMANIKKAM ,&nbsp;Elumalai Perumal VENKATESAN ,&nbsp;Sunil BISHWAKARMA ,&nbsp;Ravi KRISHNAIAH ,&nbsp;C Ahamed SALEEL ,&nbsp;Manzoore Elahi M. SOUDAGAR ,&nbsp;M.A. KALAM ,&nbsp;Muhammad Mahmood ALI ,&nbsp;Muhammad Nasir BASHIR","doi":"10.1016/j.cjac.2024.100415","DOIUrl":"10.1016/j.cjac.2024.100415","url":null,"abstract":"<div><p>Nowadays, people are highly conscious of the environment, leading to rapid growth and progress in research and innovation in eco-friendly natural fiber composites (NFCs), which are also cost-effective. The sustainable development of biodegradable NFCs obtained from renewable sources is paving the way for the replacement of synthetic fiber composites. Furthermore, researchers are focusing on enhancing the mechanical performance of NFCs for various applications. Some renewable sources, such as rice, wheat, and corn, have been used to produce polylactic acid-based NFCs. Due to their low greenhouse gas emissions and minimal energy consumption during production, NFCs are also suitable for 3D printing applications. However, they do have some drawbacks, and to overcome them, they are often blended with other NFCs. The purpose of this review article is to provide data on the physical, chemical, and mechanical properties of natural fiber-reinforced composites to assist new researchers. The central idea is that natural, biodegradable, and eco-friendly materials can replace plastics, enhancing their properties in terms of mechanical strength, thermal resistance, antimicrobial properties, water absorption, and degradability. The use of NFCs is flourishing in various fields. Every year, a substantial amount of agricultural waste with potential fibers is discarded. Some examples of waste products include fruit, including areca and tamarind. The reinforcement obtained from these materials is utilized in polymer composites for lightweight structures, the automotive industry, and domestic applications.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 7","pages":"Article 100415"},"PeriodicalIF":1.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204024000604/pdfft?md5=410b5bc0056a9146019c4cdcf6bfd8bc&pid=1-s2.0-S1872204024000604-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141946985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on the effect of processing methods on ginseng quality and key technologies for improvement","authors":"Yong-Fei QIAO ,&nbsp;Rong-Can WANG ,&nbsp;Xin-Yi WANG ,&nbsp;Ya-Li LI","doi":"10.1016/j.cjac.2024.100413","DOIUrl":"https://doi.org/10.1016/j.cjac.2024.100413","url":null,"abstract":"<div><p>As an important medicinal plant, ginseng has various pharmacological activities such as antioxidant, anti-tumor, and anti-inflammatory. The magnitude of these pharmacological activities is closely related to ginseng quality, so to improve the pharmacological activities of ginseng, it is first necessary to improve its quality. In addition to ginseng variety, cultivation, origin, harvest time, and other factors, the impact of processing on ginseng quality is also crucial. This article reviews the different methods of ginseng processing and their impact on ginseng quality, and proposes some strategies to improve ginseng quality through processing methods, aiming to provide technological support for the high-quality development of the ginseng industry.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 7","pages":"Article 100413"},"PeriodicalIF":1.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204024000586/pdfft?md5=5f905495bc7400adba238f648887f7eb&pid=1-s2.0-S1872204024000586-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141540943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of activated carbon/alginate for the concurrent removal efficiency of paracetamol and caffeine from wastewater in their binary solutions","authors":"Samar M. Mahgoub ,&nbsp;Amna A. Kotp ,&nbsp;Mahmoud A. Mohamed ,&nbsp;Haifa E. Alfassam ,&nbsp;Hassan A. Rudayni ,&nbsp;Ahmed A. Allam ,&nbsp;Rehab Mahmoud ,&nbsp;S.A. Abdel Moaty","doi":"10.1016/j.cjac.2024.100403","DOIUrl":"10.1016/j.cjac.2024.100403","url":null,"abstract":"<div><p>The increasing amount of pharmaceutical chemicals in wastewater is a concern for both public health and the environment. This research investigates the effectiveness of a new composite material made of alginate-activated carbon in removing caffeine and paracetamol from wastewater simultaneously. The composite material, produced using a simple technique, demonstrates excellent removal rates and exceptional adsorption properties for both medicinal chemicals. The structural stability and integrity of the composite are confirmed through comprehensive characterization methods including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction, energy dispersive X-ray (EDX), and BET surface area analysis. The study uses batch adsorption experiments to assess the impact of various factors, such as pH, initial concentration, and contact time, on the efficiency of caffeine and paracetamol removal. The highest values for the adsorption process were 89.63% for Paracetamol and 97.32% for Caffeine, achieved under optimal experimental conditions (pH 7, 0.1 g dose of adsorbent at elevated temperature). Additionally, Kinetic studies and thermodynamic parameters such as Δ<em>S</em>°, Δ<em>G</em>º, and Δ<em>H</em>° were calculated, indicating that the adsorption process was endothermic and spontaneous for paracetamol, and exothermic and non-spontaneous for caffeine. Seven non-linear equilibrium isotherm models were utilized to fit the experimental data for paracetamol and caffeine at pH 7 showing maximum adsorption capacities (<span><math><msub><mi>q</mi><mi>max</mi></msub></math></span>) of 606.80 and 725.05 mg/g for Paracetamol and Caffeine, respectively with a high regression coefficient (<em>R</em>²) of 0.99 using alginate-activated carbon as an adsorbent, The results demonstrate the potential of alginate-activated carbon as an effective adsorbent for the simultaneous removal of various pharmaceutical pollutants, highlighting the synergistic adsorption effects of the composite material. The study also examines the safety of the composite material, particularly in the context of potential medical applications. The study emphasizes the potential for sustainable and repeated use of the activated carbon/alginate composite, demonstrating that it maintains both its structural integrity and adsorption effectiveness after multiple cycles of use. This study evaluated the suitability of the suggested analytical method using BAGI, a metric with a unique formula. The BAGI is a complementary tool to GAPI, Complex GAPI, AGREE, AGREE prep, and ESA. A major focus is on the practical elements of white analytical chemistry centered around \"blue”.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 7","pages":"Article 100403"},"PeriodicalIF":1.2,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204024000483/pdfft?md5=847b086ff884c88e3392d14d887dcfd0&pid=1-s2.0-S1872204024000483-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141279608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A pilot study on the mechanism of Guasha in treating Parkinson's disease based on molecular level and ultra-trace proteomics analysis","authors":"Qiuqin WANG ,&nbsp;Li ZHANG ,&nbsp;Heng WENG ,&nbsp;Qing WANG ,&nbsp;Penglu CHEN ,&nbsp;Hua CHEN ,&nbsp;Rongrong JIANG ,&nbsp;Guihua XU ,&nbsp;Yamei BAI ,&nbsp;Xi CHEN","doi":"10.1016/j.cjac.2024.100402","DOIUrl":"10.1016/j.cjac.2024.100402","url":null,"abstract":"<div><p>Guasha is a widely applied non-pharmacological therapy of traditional Chinese medicine (TCM), which has been proved to be effective in the treatment of Parkinson's disease (PD). The objective of this study was to explore the mechanisms of Guasha therapy in the pathological processes including neuroinflammation in PD. After 3 Guasha courses, a reduction in α-synuclein aggregation and alleviation of microglia activation were observed in the substantia nigra pars compacta (SNpc). Furthermore, levels of TNF-α, IL-1β and IFN-γ in the SNpc decreased, while plasma TGF-β, IL-4 and IL-10 increased. Moreover, both TH protein and mRNA levels in the SNpc, as well as dopamine levels in the striatum, exhibited an increase. The proteomics analysis results based on plasma-derived exosomes revealed a total of 943 differentially expressed proteins identified. Compared to the model group, the Guasha group screened for 82 differential proteins, with 30 upregulated and 52 downregulated. The improvement of pathological changes in the PD model mice treated with Guasha primarily involves biological processes such as oxidative stress, immune response and inflammation, and cellular structure regulation. It involves signaling pathways related to aldosterone synthesis and secretion, adrenergic signaling in myocardial cells, COVID-19-related inflammatory signaling, and neurotrophic signaling. Collectively, the mechanisms of Guasha for treating PD might be closely related to inhibiting microglial cell activation-mediated neuroinflammation, regulating oxidative stress, cellular structure, aldosterone synthesis and secretion-mediated electrolyte balance, as well as noradrenergic signaling-mediated neuroprotection. These findings provided new insight for Guasha in treating PD and would potentially enhance therapeutic interventions.</p></div>","PeriodicalId":277,"journal":{"name":"Chinese Journal of Analytical Chemistry","volume":"52 6","pages":"Article 100402"},"PeriodicalIF":1.2,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1872204024000471/pdfft?md5=7d50d14248abd8f3a4ed8fdc0808e940&pid=1-s2.0-S1872204024000471-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141136710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}