基于波谱效应关系鉴定胆汁水提物中有效抗氧化成分

IF 1.2 4区化学 Q4 CHEMISTRY, ANALYTICAL

Chinese Journal of Analytical Chemistry Pub Date : 2024-12-01 DOI:10.1016/j.cjac.2024.100453

Congyou DENG , Xinhe LEI , Minyou HE , Guowei LI , Xiangdong CHEN , Dongmei SUN , Qing DING , Jiang MENG , Xiaozhou JIA , Minyu WANG

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引用次数: 0

摘要

目前对莪术的研究主要集中在对其成分及药理作用变化的分析上。水提物的化学成分、抗氧化、抗热惊厥的物质基础和作用机制尚不清楚。本研究利用指纹图谱-药理学关系，探讨了白芷胆汁水提物抗氧化作用的药效学和药理物质基础。方法通过确定共同峰，记录峰面积，评价不同批次间的相似度，建立莪术水提物的高效液相色谱指纹图谱。测定每批炮制的莪术提取物的抗氧化能力，并在指纹图谱的基础上进行光谱效应分析和筛选。通过对分离得到的化合物进行评价，验证了莪术水提物的抗氧化活性。结果采用紫外分光光度法和蒸发光散射法建立了15批缬草胆水提物的HPLC指纹图谱。共有22个共同峰，其中11个为尿嘧啶、次黄嘌呤、黄嘌呤、尿苷、鸟苷、腺苷、五叶草苷、异麦草苷、牛磺酸氢去氧胆酸、牛磺酸烯去氧胆酸和甘氨酸烯去氧胆酸。光谱效应关系分析表明，五叶草皂苷、异麦草皂苷、牛磺酸鹅去氧胆酸和糖鹅去氧胆酸可能是该提取物的有效抗氧化成分，这四种成分在单独测试时均表现出剂量依赖性的抗氧化作用。结论建立了莪术水提物的指纹图谱，测定了提取物的抗氧化能力，并通过谱效关系分析鉴定了有效抗氧化成分。

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Identifying the active antioxidant ingredients in Arisaema Cum Bile aqueous extract on the basis of spectrum-effect relationships

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Identifying the active antioxidant ingredients in Arisaema Cum Bile aqueous extract on the basis of spectrum-effect relationships

At present, the research on Arisaema Cum Bile mainly focuses on the analysis of its components and changes in pharmacological effects. The chemical composition of the water extract, the material basis and mechanism of its anti-oxidation and anti-febrile convulsion are still unclear. In this study, fingerprint-pharmacological relationships were used to determine the pharmacodynamic and pharmacological material basis of the antioxidant effects of Arisaema Cum Bile aqueous extract.

Methods

The High Performance Liquid Chromatography (HPLC) fingerprint of the aqueous extract of Arisaema Cum Bile was established by determining the common peaks, recording the peak areas and evaluating the similarities between different batches. Moreover, the antioxidant capacity of each batch of processed Arisaema Cum Bile extracts was determined, and spectrum-effect relationship analysis and screening were conducted on the basis of the fingerprint. The antioxidant activities of the Arisaema Cum Bile aqueous extract ingredients were verified by evaluating the isolated compounds.

Results

The HPLC fingerprints of 15 batches of aqueous extracts of Arisaema Cum Bile aqueous extracts were established via ultraviolet (UV) and evaporative light scattering detection methods. There was a total of 22 common peaks, 11 of which were identified as uracil, hypoxanthine, xanthine, uridine, guanosine, adenosine, shaftoside, isoschaftoside, taurohyodeoxycholic acid, taurochenodeoxycholic acid and glycinechenodeoxycholic acid. Spectrum-effect relationship analysis revealed that shaftoside, isoschaftoside, taurochenodeoxycholic acid and glycochenodeoxycholic acid may be the active antioxidant ingredients in this extract, which was verified since all four ingredients displayed dose-dependent antioxidant effects when tested individually.

Conclusion

A fingerprint of the aqueous extract of Arisaema Cum Bile aqueous extract was established, the antioxidant capacities of the extracts were measured, and the active antioxidant ingredients were identified through spectrum-effect relationship analysis.

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来源期刊

Chinese Journal of Analytical Chemistry 化学-分析化学

CiteScore

3.60

自引率

25.00%

发文量

17223

审稿时长

35 days

期刊介绍： Chinese Journal of Analytical Chemistry(CJAC) is an academic journal of analytical chemistry established in 1972 and sponsored by the Chinese Chemical Society and Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. Its objectives are to report the original scientific research achievements and review the recent development of analytical chemistry in all areas. The journal sets up 5 columns including Research Papers, Research Notes, Experimental Technique and Instrument, Review and Progress and Summary Accounts. The journal published monthly in Chinese language. A detailed abstract, keywords and the titles of figures and tables are provided in English, except column of Summary Accounts. Prof. Wang Erkang, an outstanding analytical chemist, academician of Chinese Academy of Sciences & Third World Academy of Sciences, holds the post of the Editor-in-chief.