Chemosphere最新文献

{"title":"Highly selective bio-functionalized graphene-based sponges for adsorption and degradation of polycyclic aromatic hydrocarbon mixtures","authors":"Mahsa Moayedi,&nbsp;Nariman Yousefi","doi":"10.1016/j.chemosphere.2025.144321","DOIUrl":"10.1016/j.chemosphere.2025.144321","url":null,"abstract":"<div><div>Xenobiotic pollutants such as polycyclic aromatic hydrocarbons (PAHs), originating from the incomplete combustion of organic substances, yield harmful effects on both the environment and public health. Existing research highlights that ligninolytic enzymes, including laccase, exhibit the capability to degrade different PAHs to varying degrees. Enzyme immobilization on a support enhances their suitability for industrial uses, typically leading to improved storage and operational stability. This study aims to enhance the elimination of PAHs such as naphthalene, anthracene, phenanthrene and pyrene and their mixture from water by merging the biocatalytic activity of laccase with the high adsorption capacity of a reduced graphene oxide (rGO) sponge. Our findings revealed that as the molecular weight and hydrophobic properties of PAHs increased, their affinity towards the rGO sponges became more pronounced. Conversely, it was noted that the elimination of naphthalene exhibited remarkable enhancement (achieving 75 % removal after 48 h individually, and 82 % removal in PAH mixtures), demonstrating faster removal kinetics in contrast to other PAHs. This improvement was attributed to the utilization of a bio-functionalized rGO sponge, indicating the notable role of immobilized laccase in the degradation of naphthalene. As observed, certain PAHs in the mixture were more susceptible to oxidation and enzymatic degradation, while those with a higher affinity for adsorption onto the rGO surface demonstrated reduced degradability. This selective mechanism effectively treated specific PAHs based on their structural characteristics, thus enhancing the overall efficiency in removing diverse PAH contaminants in mixtures. The results regarding PAH degradation by-products indicated that laccase primarily converted anthracene into 9,10-anthraquinone, most of which were adsorbed and subsequently eliminated by the rGO sponge acting as the enzyme's support.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"377 ","pages":"Article 144321"},"PeriodicalIF":8.1,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developmental toxicity of formulated insecticide mixture containing imidacloprid and beta-cyfluthrin in fish: Insights using zebrafish","authors":"Livia Pitombeira de Figueirêdo,&nbsp;Felipe Cirqueira,&nbsp;Bianca Leite Carnib de Sousa,&nbsp;Joseph Mamboungou,&nbsp;Thiago Lopes Rocha","doi":"10.1016/j.chemosphere.2025.144314","DOIUrl":"10.1016/j.chemosphere.2025.144314","url":null,"abstract":"<div><div>Insecticides are critical in controlling pests and disease vectors. However, there is still a lack of ecotoxicological studies using commercial formulations of insecticides containing active ingredients. The study aimed to evaluate the developmental toxicity of a commercial insecticide mixture (imidacloprid [IMI] + beta-cyfluthrin [β-CYF]). Mortality, hatching rate, spontaneous contraction, heartbeat, morphological changes, reactive oxygen species (ROS), skeletal development, and locomotor behavior of zebrafish were analyzed. Embryos were exposed to imidacloprid (IMI) and β-cyfluthrin (β-CYF) in the following ratios: 0.001 mg IMI·L⁻¹ + 0.000125 mg β-CYF·L⁻¹ (C1); 0.01 mg IMI·L⁻¹ + 0.00125 mg β-CYF·L⁻¹ (C2); 0.1 mg IMI·L⁻¹ + 0.0125 mg β-CYF·L⁻¹ (C3); 1.0 mg IMI·L⁻¹ + 0.125 mg β-CYF·L⁻¹ (C4); 10.0 mg IMI·L⁻¹ + 1.25 mg β-CYF·L⁻¹ (C5) for 144 h. The results showed a mortality of 50 % of organisms in the C5 concentration. Embryos exposed to C1 and C3 showed tachycardia and hatched early compared to the negative control, indicating cardiotoxic and embryotoxic effects. The two highest concentrations tested (C4 and C5) induced evident morphological changes (yolk sac and pericardial edema, and spine alterations), and skeletal toxicity (absence of cartilage and bone formation), along with decreased larval swimming behavior. Also, the formulated insecticide (C1) increased ROS levels in zebrafish larvae. Results showed that the formulated insecticide containing IMI and β-CYF induces several toxic effects on developing zebrafish, indicating its environmental risk to aquatic organisms.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"377 ","pages":"Article 144314"},"PeriodicalIF":8.1,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil electrokinetic remediation to restore mercury-polluted soils: A critical review","authors":"Ahmed Abou-Shady ,&nbsp;Heba El-Araby","doi":"10.1016/j.chemosphere.2025.144336","DOIUrl":"10.1016/j.chemosphere.2025.144336","url":null,"abstract":"<div><div>Mercury (Hg) is one of the top chemicals of concern to the World Health Organization and a potentially hazardous trace element in the environment. Hg pollution is a serious issue, and due to its metallic nature or poor soluble mineral properties (such as some sulfides), Hg pollution demands chemical additives to increase its solubility before removal. Different viewpoints were used to illustrate how electrokinetic remediation technology could be used to remove Hg from polluted soil, including 1) cathode approaching, 2) pulsed electric field, 3) chemical additives, 4) coupling with phytoremediation and bioremediation, 5) integration of adsorption and a permeable reactive barrier, and 6) electrodialytic design, operation factors, and electrode materials. To collect the relevant publications during the last 32 years (1993–2024), search words such as “Soil electrokinetic &amp; Hg” were entered into six search engines. Only a few articles have been published concerning electrokinetic remediation of Hg. The effectiveness of iodide in removing Hg was recently demonstrated by testing several chelating compounds. Iodide added to the soil solution migrated Hg toward the anode, demonstrating the presence of Hg iodide anionic complexes. By contrast, anionic Hg chloride complexes did not dominate, as evidenced by Hg migration toward the cathode when chloride was introduced to the soil. Although electrokinetic design remediation has advanced significantly, most studies have focused on Hg remediation using the soil electrokinetic approach, which is a conventional design. One study used the electrodialytic design, and one electrode arrangement (rectangular and hexagonal). For the readers' advantage, further developments for the electrokinetic remediation of Hg from soil were also suggested.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"377 ","pages":"Article 144336"},"PeriodicalIF":8.1,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rare earth elements and health risk assessment of road dust from the vicinity of coal fired thermal power plants","authors":"Jovana Roganović ,&nbsp;Dubravka Relić ,&nbsp;Milana Zarić ,&nbsp;Mira Aničić Urošević ,&nbsp;Inga Zinicovscaia ,&nbsp;Konstantin Ilijević ,&nbsp;Nenad M. Zarić","doi":"10.1016/j.chemosphere.2025.144329","DOIUrl":"10.1016/j.chemosphere.2025.144329","url":null,"abstract":"<div><div>As emerging pollutants, rare earth elements (REEs) have been explored in different environmental samples. This is the first study to use road dust samples to monitor REEs form the vicinity of thermal power plant (TPPs). Road dust samples were collected from 17 locations (main and side roads) in a 15 km radius surrounding two coal-fired TPP (TPP Kostolac A &amp; B, Serbia). Concentrations of nine REEs (Sc, La, Ce, Nd, Sm, Eu, Tb, Dy, Yb) were measured in the road dust samples (f &lt; 63 μm, easily resuspended fraction size) using instrumental neutron activation analysis (INAA). We have found that the concentrations of REEs do not depend on the distance of the sampling location from TPP. There were no statistically significant differences between the main road and side road samples suggest that traffic is not the main source of REE in the studied area. Principal component analysis, hierarchical cluster analysis as well as geo-accumulation index (I_geo) and enrichment factors (EF) point to an enrichment with Dy of road dust samples collected in September. The road dust samples do not pose any harm to human health in the tested area, as shown by a hazard index of less than 0.1. Despite the low REE risk, it is important to consider the possibility of negative health consequences, mainly because these samples may contain numerous other organic and inorganic pollutants.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"377 ","pages":"Article 144329"},"PeriodicalIF":8.1,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis of platinum and palladium nanoparticles from spent automotive catalyst leachate using bioreduction","authors":"Salman Karim,&nbsp;Yen-Peng Ting","doi":"10.1016/j.chemosphere.2025.144340","DOIUrl":"10.1016/j.chemosphere.2025.144340","url":null,"abstract":"<div><div>Metal-bearing solid waste is considered a secondary source of precious metals. Spent automotive catalyst (SAC) contains significant quantities of platinum group metals (PGM). The biorecovery of these metals from SAC is gaining widespread attention due to its economic and environmental advantages. However, most of the studies focused on the bioextraction of these metals and ignored their separation and precipitation from leach liquor. The few studies that investigated their separation used model synthetic solutions instead of real waste to recover these metals. This study used the bioreduction pathway to recover Pt and Pd from SAC leach liquor. A Gram-negative, metallophillic, and heavy metal-resistant bacterium <em>Cupriavidus metallidurans</em> is used to biosynthesize Pt and Pd nanoparticles. <em>C. metallidurans</em> undergo bioreduction which is an enzymatically-assisted metal precipitation process to biofabricate the Pt and Pd nanoparticles intracellularly, on the cell surface, and extracellularly. The viable cells of <em>C. metallidurans</em> showed a bioreduction efficiency of 65 % and 52 % of Pt (II) and Pd (II), respectively, from SAC leachate. Overall, this study shows the potential and efficacy of the biorecovery of Pt and Pd and the green synthesis of Pt and Pd nanoparticles from metal-bearing solid waste.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"377 ","pages":"Article 144340"},"PeriodicalIF":8.1,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomics identified distinct molecular-level responses in Daphnia magna after exposure to phenanthrene and its oxygen and nitrogen containing analogs","authors":"Salwa Hajir ,&nbsp;Karl J. Jobst ,&nbsp;Sonya Kleywegt ,&nbsp;André J. Simpson ,&nbsp;Myrna J. Simpson","doi":"10.1016/j.chemosphere.2025.144334","DOIUrl":"10.1016/j.chemosphere.2025.144334","url":null,"abstract":"<div><div>The prevalence of polycyclic aromatic hydrocarbons and their oxygenated and nitrogen containing analogs in freshwater ecosystems are of concern due to their reported toxicity to several aquatic species including <em>Daphnia magna</em>. This study explored the molecular-level responses of phenanthrene (PHEN), 9,10-phenanthrenequinone (PHQ), and phenanthridine (PN) as little is known about the impacts of these pollutants on the metabolic profile of <em>D. magna</em>. For this purpose, <em>D</em>. <em>magna</em> was exposed to three sub-lethal concentrations of these pollutants for 24 h. To assess molecular-level responses, 52 polar metabolites were extracted from individual adult daphnids, and analyzed using a mass spectrometry-based targeted metabolomics approach. Exposure to PN resulted in the most statistically significant changes to the metabolic profile of <em>D. magna</em> followed by PHQ, and then PHEN exposures. After PN exposure, the biochemical pathway analysis showed that all exposure concentrations shared 21 perturbed metabolic pathways. However, the number of disrupted metabolic pathways increased with increasing exposure concentrations for PHEN and PHQ. The results suggest that PN and PHQ exposures are more disruptive due to the presence of reactive functional groups when compared to PHEN exposure. For the tested concentration ranges, the findings indicate that exposure to PN resulted in non-monotonic disruptions across exposure concentrations. In contrast, exposure to PHEN and PHQ elicited perturbations that were concentration-dependent. Although the reported median effective concentration (EC₅₀) for PN is higher than PHEN and PHQ, our data shows that metabolomics captures molecular-level changes that may not be detected by traditional toxicity metrics.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"377 ","pages":"Article 144334"},"PeriodicalIF":8.1,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adding the stoichiometric ratio between oxygen consumed per oxidized carbon (O/C) in the mathematical model of aquatic macrophyte mineralization","authors":"Deivid Souza Silva ,&nbsp;Elineide Eugênio Marques ,&nbsp;Marcela Bianchessi da Cunha Santino ,&nbsp;Irineu Bianchini Jr.","doi":"10.1016/j.chemosphere.2025.144346","DOIUrl":"10.1016/j.chemosphere.2025.144346","url":null,"abstract":"<div><div>This study was proposed to include the stoichiometric relationship between oxygen consumed by oxidized carbon (O/C) in the aerobic mineralization pathways of aquatic plants (i.e., oxidations of labile particulate organic carbon, dissolved organic carbon, and recalcitrant particulate organic carbon). This addition brings together two mathematical models used to describe the decomposition of macrophytes. This stoichiometry makes it possible to deduce the oxygen consumption of mineralization, depending on the composition of the detritus. It also allows the inference of the predominant catabolic routes selected by microorganisms. This type of information is not available in kinetic studies of mathematical modeling of macrophyte decomposition. To this end, two experiments were carried out (1st: mass loss kinetics; 2nd: oxygen consumption) with 5 macrophytes species: <em>Echinodorus tenellus</em>, <em>Hydrocotyle verticillata</em>, <em>Ludwigia sedioides</em>, <em>Najas macrocarpa</em> and <em>Pontederia parviflora</em>. After parameterizing the kinetic model (including O/C relationships), the simulated results were compared with those from the 2nd experiment to evaluate the validity of the proposed model. A comparison of the results indicated the success of the proposal (r² &gt; 0.97); O/C ratio values varied between 0.08 and 2.51. Mineralization of labile and soluble organic compounds tends to produce short-term oxygen consumption, while those of recalcitrant fractions tend to generate low-intensity, long-term consumption. Determinations of O/C relationships improve predictions of oxygen balances in aquatic environments, as in these environments, the cycling rate and reactivity of organic compounds change depending on the main composition of the plant resource, generating specific oxygen demands.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"377 ","pages":"Article 144346"},"PeriodicalIF":8.1,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional gene array and non-target soil microorganisms in nanopesticides captan@ZnO35–45nm and captan@SiO2 20–30nm environmental risk assessment","authors":"Sławomir Sułowicz ,&nbsp;Sławomir Borymski ,&nbsp;Mateusz Dulski ,&nbsp;Anna Nowak ,&nbsp;Anna Markowicz","doi":"10.1016/j.chemosphere.2025.144347","DOIUrl":"10.1016/j.chemosphere.2025.144347","url":null,"abstract":"<div><div>Currently there are no widely recognized standards for assessing the environmental risk of nanopesticides. Therefore, whether they are safer than conventional pesticide products remains open to discussion. We used non-target soil microorganisms as indicators of environmental change and applied functional gene arrays (FGAs) using GeoChip 5.0S targeting 151 000 genes involved in ecologically relevant functions. We synthesized nanofungicides in which the active substance captan was bound to inorganic nanocarriers (ZnO and SiO₂) to examine the functional capabilities of microbial communities. During a 100-day microcosm study, changes in soil were compared to the effect of pesticide and nanocarriers alone. Based on 72 functional gene diversity profiles, we conducted environmental risk assessment of nanopesticides. Nanoagrochemicals affected the alpha and beta diversity of microbial functional genes, and the most profound negative effect was detected for captan, impacting carbon cycling and the organic remediation process from day 30. Additionally, the effect of nanopesticides changed during the experiment. On day 42, the effect was nanocarrier-dependent, and an increase of genes involved in denitrification (<em>nirS, norB)</em>, archaeal conversion of N₂ to NH₃ and fungal N-assimilation was observed, especially for SiO₂-treated set-ups. After 100 days, the negative effect was mainly related to the active substance released from the nanocarrier impacting the nitrate reductase gene (<em>narG</em>) and genes from the denitrification and nitrogen fixation subcategory. Analysis of microarrays did not indicate a recovery process for carbon cycling. Moreover, pesticide and nanoagrochemicals affected arsenic detoxification (<em>aoxB</em>, <em>arsM</em>, <em>arsC</em>), which may lead to an elevation of toxic As(III) availability. Our study indicated that FGAs are a sensitive method, revealing long-term changes previously undetected by other methods, including next-generation sequencing (NGS). This is the first study to confirm the usefulness of GeoChips for the evaluation of microbial redundancy as an important factor for fair environmental risk assessment of nanopesticides.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"377 ","pages":"Article 144347"},"PeriodicalIF":8.1,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ion exchange membranes in environmental applications: Comprehensive review","authors":"Yokubjon Bozorov ,&nbsp;Khait Turaev ,&nbsp;Rustam Alikulov ,&nbsp;Masud Karimov ,&nbsp;Bakhriddin Muminov ,&nbsp;Elyor Berdimurodov ,&nbsp;Ilyos Eliboev ,&nbsp;Muslum Demir ,&nbsp;Adeyinka Sikiru Yusuff ,&nbsp;Natarajan Elangovan","doi":"10.1016/j.chemosphere.2025.144327","DOIUrl":"10.1016/j.chemosphere.2025.144327","url":null,"abstract":"<div><div>Ion exchange membranes (IEMs) are transformative materials in environmental and industrial applications, offering selective ion transport capabilities crucial for water desalination, wastewater treatment, energy generation, and resource recovery. Recent advancements have focused on developing nanocomposite and organic-inorganic hybrid membranes, integrating materials like graphene oxide, silica, and carbon nanotubes to enhance mechanical strength, thermal stability, and chemical resistance. These innovations have yielded remarkable results, such as achieving 77.9 % energy conversion efficiency and current densities of 1000 mA/cm² in seawater electrolysis systems. Additionally, advanced IEMs demonstrate significant improvements in selective ion removal, with lithium recovery efficiencies reaching 93 % and fluoride reduction below WHO guidelines. Despite these successes, challenges like fouling, chemical degradation, high costs, and scalability barriers remain. Future research directions emphasize sustainability, with a focus on biopolymer-based membranes, renewable energy integration, and computational modeling. By addressing these challenges, IEMs can significantly contribute to global environmental sustainability and resource efficiency. IEMs are vital in energy generation, enabling ion transport in fuel cells (PEMFCs, AEMFCs) for clean energy, redox flow batteries (VRFBs) for efficient energy storage, and electrolyzers (PEMELs, AEMELs) for hydrogen production. They also support salinity gradient power in reverse electrodialysis (RED) and pressure-retarded osmosis (PRO) and facilitate CO₂ electroreduction (CO₂RR) for carbon-neutral fuel production. This review (covering 2020–2024 publication years) explores recent developments in IEM technology, highlighting their applications, challenges, and future prospects in addressing global environmental and industrial challenges.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"377 ","pages":"Article 144327"},"PeriodicalIF":8.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactions of erythromycin and an antibiotic mixture with the gut microbiome of juvenile rainbow trout","authors":"Phillip J. Ankley ,&nbsp;Jonathan Challis ,&nbsp;Pu Xia ,&nbsp;Yufeng Gong ,&nbsp;Yutong Zhou ,&nbsp;Markus Hecker ,&nbsp;John P. Giesy ,&nbsp;Markus Brinkmann","doi":"10.1016/j.chemosphere.2025.144263","DOIUrl":"10.1016/j.chemosphere.2025.144263","url":null,"abstract":"<div><div>Erythromycin (ERY) is a commonly used antibiotic found in wastewater effluents and the environment globally. Due to the bioactivity by which they kill and prevent bacterial growth, ERY and other antibiotics may have significant unwanted impacts on the gut microbiome of fishes. The overall objective of this project was to assess effects on the gut microbiome in response to exposure to ERY alone or in a mixture with other common antibiotics, which was accomplished in two experiments. The objectives of experiment 1 as a pilot study were to understand uptake and depuration of ERY in juvenile rainbow trout (RBT) over a 7-d exposure to three concentrations of ERY followed by a 7-d depuration period. Furthermore, throughout the study changes in gut microbiome were assessed. In experiment 2, an identical experimental design was used to assess the effects of a mixture of antibiotics containing, in addition to ERY, 100 μg/g each of ampicillin, metronidazole, and ciprofloxacin. In that study, three matrices were analyzed, with gut collected for 16S rRNA metabarcoding, blood plasma for non-targeted metabolomics, and brain tissue for mRNA-seq analysis. ERY was relatively quickly depurated from fish and gut microbiome dysbiosis was observed at 7 d after exposure, with a slight recovery after the 7-d depuration period. A greater number of plasma metabolites was dysregulated at 14 d compared to 7 d revealing distinct temporal dynamics compared to gut microbiome dysbiosis. Furthermore, several transformation products of antibiotics and biomarker metabolites were observed in plasma due to antibiotic exposure. The transcriptome of the brain was only slightly altered due to antibiotic exposure. Results of these studies will help inform aquaculture practitioners and risk assessors when assessing the potential impacts of antibiotics present in fish feed and the environment, with implications for host health.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"377 ","pages":"Article 144263"},"PeriodicalIF":8.1,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143683929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}