ChemospherePub Date : 2025-05-20DOI: 10.1016/j.chemosphere.2025.144450
Amir Hossein Ghanbari , Samira Fathi , Mohammad Hossein Sheikhi , Zoheir Kordrostami
{"title":"Fabrication of non-enzymatic glucose biosensor based on CuS/Ni3S4 reinforced microflowers","authors":"Amir Hossein Ghanbari , Samira Fathi , Mohammad Hossein Sheikhi , Zoheir Kordrostami","doi":"10.1016/j.chemosphere.2025.144450","DOIUrl":"10.1016/j.chemosphere.2025.144450","url":null,"abstract":"<div><div>Diabetes is a prevalent and life-threatening condition that is caused by uncontrolled changes in glucose concentration in the human body; therefore, daily monitoring of blood glucose concentration is a big necessity for almost 500 million diabetic patients worldwide. In this work, a low-cost and eco-friendly non-enzymatic electrochemical glucose biosensor based on copper sulfide (CuS) and nickel sulfide (Ni<sub>3</sub>S<sub>4</sub>) microstructures has been introduced. CuS Microflowers (MFs) were synthesized using a solvothermal method and reinforced by Ni<sub>3</sub>S<sub>4</sub> nanoparticles (NPs) with a simple and one-step hydrothermal procedure. Cyclic voltammetry (CV) and chronoamperometry (CA) were employed to evaluate the electrochemical performance of the synthesized non-enzymatic glucose biosensors. Furthermore, the reinforced microflowers (RMFs) based biosensor exhibited an excellent improvement with selectivity toward glucose molecules, an LOD of 2.5 μM, and a great response time of less than 5 s. RMFs based biosensor showed two linear ranges of 0–1 mM and 1–5 mM with sensitivities of 303.85 μA/mM.cm<sup>2</sup> and 164.85 μA/mM.cm<sup>2</sup> respectively.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"382 ","pages":"Article 144450"},"PeriodicalIF":8.1,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144090043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ChemospherePub Date : 2025-05-20DOI: 10.1016/j.chemosphere.2025.144490
Qirat Yameen , Muhammad Ikram , Sawaira Moeen , Muhammad Imran , Anwar Ul-Hamid , Ghafar Ali , Souraya Goumri-Said , Mohammed Benali Kanoun
{"title":"Enhanced electrocatalytic OER performance of Ba/CS-CoFe2O4 ternary heterostructure catalyst: Experimental and theoretical insights","authors":"Qirat Yameen , Muhammad Ikram , Sawaira Moeen , Muhammad Imran , Anwar Ul-Hamid , Ghafar Ali , Souraya Goumri-Said , Mohammed Benali Kanoun","doi":"10.1016/j.chemosphere.2025.144490","DOIUrl":"10.1016/j.chemosphere.2025.144490","url":null,"abstract":"<div><div>Herein, a ternary heterostructure catalyst Ba/CS-CoFe<sub>2</sub>O<sub>4</sub> (barium/chitosan-doped Cobalt ferrite) was developed by a straightforward co-precipitation technique to investigate oxygen evolution reaction (OER) activity. Varying quantities (2 and 4 wt %) of Ba and a fixed amount (3 wt %) of CS were doped to modify the surface area, porosity, crystallite size, and stability of CoFe<sub>2</sub>O<sub>4</sub>. Comprehensive characterizations revealed multiple phases, polycrystalline behavior, enhanced absorption, structural defects, and nanorods overlapping nanoparticles (NPs) like the morphology of Ba/CS-CoFe<sub>2</sub>O<sub>4</sub>. Furthermore, the experimental results revealed that 2 wt % of Ba/CS-CoFe<sub>2</sub>O<sub>4</sub> exhibited superior electrocatalytic activity with the highest kinetics and ECSA (electrochemically active surface area) for the OER process in 1 M KOH. To further elucidate the OER performance, density functional theory (DFT) calculations were conducted. The optimized CoFe<sub>2</sub>O<sub>4</sub> structure was confirmed to have a cubic Fd-3m symmetry, with a calculated bandgap energy (E<sub>g</sub>) of 1.62 eV, closely matching experimental data. Adsorption energy calculations showed that Ba/CS doping significantly improved the binding strength of OH intermediates on the CoFe<sub>2</sub>O<sub>4</sub> (100) surface, highlighting the role of dopants in enhancing surface reactivity. These findings demonstrate the potential of Ba/CS doping to optimize the electronic, structural, and surface properties of CoFe<sub>2</sub>O<sub>4</sub> for efficient OER electrocatalysis, paving the way for novel electrochemical catalyst design.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"382 ","pages":"Article 144490"},"PeriodicalIF":8.1,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144090041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ChemospherePub Date : 2025-05-20DOI: 10.1016/j.chemosphere.2025.144492
Graziella Orso, Roberta Imperatore, Rosaria Sciarrillo, Marina Paolucci
{"title":"Fish skin for water quality assessment","authors":"Graziella Orso, Roberta Imperatore, Rosaria Sciarrillo, Marina Paolucci","doi":"10.1016/j.chemosphere.2025.144492","DOIUrl":"10.1016/j.chemosphere.2025.144492","url":null,"abstract":"<div><div>Pollution and the resulting decline in water quality pose a serious issue for aquatic biodiversity. Therefore, biomonitoring strategies to assess water quality need to be improved. In this study, we evaluated the possible use of fish skin histology as biomarker to monitor the water quality. Five fish species (<em>Salmo trutta</em>, <em>Anguilla anguilla</em>, <em>Leuciscus cephalus</em>, <em>Barbus barbus</em> and <em>Rutilus rubilio</em>) were collected from 32 river sites in the Campania region (Southern Italy), located within/outside Natura 2000 network. Body Condition Factor (BCF), Epidermis Morphological Index (EMI), Mucous Cell Index (MCI), epidermis thickness, and mucous cell size were analyzed in relation to the river site Ecological Quality Class (EQC). Negative correlation between BCF and EQC was found for <em>S. trutta</em> and <em>A. anguilla</em>, while a positive correlation was observed for <em>L. cephalus</em> and <em>B. barbus</em>. Skin histological results highlighted positive correlation between EMI and EQC in <em>S. trutta</em>, <em>A. anguilla,</em> and <em>R. rubilio,</em> while positive correlation between MCI and EQC was observed in <em>S. trutta</em>, <em>L. cephalus</em>, <em>B. barbus,</em> and <em>R. rubilio</em>. Epidermis thickness was negatively correlated with EQC in <em>S. trutta</em>, <em>L. cephalus</em>, and <em>B. barbus,</em> and positively correlated in <em>A. anguilla. M</em>ucous cells size appeared negatively correlated with EQC in <em>L. cephalus</em> and <em>R. rubilio</em> and positively correlated in <em>A. anguilla.</em> The findings of this study indicate that skin histology could be a sensitive and useful biomarker to assess water quality, suggesting its integration in biomonitoring programs. Species-specific responses need to be considered to obtain a more reliable water quality assessment.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"382 ","pages":"Article 144492"},"PeriodicalIF":8.1,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144090042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ChemospherePub Date : 2025-05-17DOI: 10.1016/j.chemosphere.2025.144484
João Barreira , Jeremie Garnier , Bruno Cunha , Myller Souza Tonhá , Elton Dantas , Remi Freydier , Wilson Machado , Marly Babinski , Rafael de Araujo , Daniel F. Araújo
{"title":"Zinc stable isotopes in mangrove crabs as tracers of anthropogenic contamination in a tropical estuary","authors":"João Barreira , Jeremie Garnier , Bruno Cunha , Myller Souza Tonhá , Elton Dantas , Remi Freydier , Wilson Machado , Marly Babinski , Rafael de Araujo , Daniel F. Araújo","doi":"10.1016/j.chemosphere.2025.144484","DOIUrl":"10.1016/j.chemosphere.2025.144484","url":null,"abstract":"<div><div>Our study presents a novel approach for tracing anthropogenic zinc (Zn) bioaccumulation in mangrove crabs using Zn stable isotope compositions (δ<sup>66</sup>Zn). We analyzed δ<sup>66</sup>Zn and elemental composition in crab tissues from Brazilian mangroves within a coastal lagoon historically impacted by electroplating waste. Crabs from the mangrove area near the old electroplating plant exhibited the highest Zn concentrations in both muscle and hepatopancreas, likely reflecting the high Zn bioavailability in these sediments. In contrast, crabs from other mangrove sites showed minimal variations in Zn concentrations, despite prominent differences in sediment Zn levels. This suggests that crabs regulate their internal Zn concentrations within a specific range, not correlating with external Zn bioavailability. The tissues of crabs from contaminated sites bore heavier δ<sup>66</sup>Zn, consistent with the δ<sup>66</sup>Zn left by industrial Zn pollution in sediments. However, δ<sup>66</sup>Zn did not correlate directly with either bioaccumulated Zn or sediment Zn levels, making it challenging to distinguish between stations with varying contamination degrees. Shifting δ<sup>66</sup>Zn in crab tissues toward lighter isotopes compared to sedimentary Zn indicates the influence of diet, internal biological fractionation, specific bioaccumulation pathways, or additional Zn sources. Biological fractionation within crabs likely caused muscle tissues to be isotopically lighter than the hepatopancreas, especially in moderately contaminated areas. This first systematic study of δ<sup>66</sup>Zn within mangrove crabs highlights the need for further research to fully understand isotopic variations in crab tissues and their relationship with environmental, ecological, and physiological factors. Initial findings suggest that sources, bioaccumulation routes, and regulatory mechanisms shape δ<sup>66</sup>Zn within crab tissues.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"382 ","pages":"Article 144484"},"PeriodicalIF":8.1,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ChemospherePub Date : 2025-05-17DOI: 10.1016/j.chemosphere.2025.144475
Zeni Anggraini , Gustri Nurliati , Hendra Adhi Pratama , Heru Sriwahyuni , Raden Sumarbagiono , Nazhira Shadrina , Mirawaty Mirawaty , Niken Siwi Pamungkas , Zico Pratama Putra , Muhammad Yusuf
{"title":"A critical review about phytoremediation of heavy metals and radionuclides: from mechanisms to post-remediation strategies","authors":"Zeni Anggraini , Gustri Nurliati , Hendra Adhi Pratama , Heru Sriwahyuni , Raden Sumarbagiono , Nazhira Shadrina , Mirawaty Mirawaty , Niken Siwi Pamungkas , Zico Pratama Putra , Muhammad Yusuf","doi":"10.1016/j.chemosphere.2025.144475","DOIUrl":"10.1016/j.chemosphere.2025.144475","url":null,"abstract":"<div><div>Phytoremediation has emerged as an environmentally friendly and cost-effective solution for mitigating heavy metal and radionuclide contamination in soil and water. While extensive research has been conducted on phytoremediation mechanisms and the effectiveness of various plant species in pollutant uptake, limited attention has been given to the crucial aspect of post-remediation biomass management, particularly for biomass containing heavy metals and radionuclides. This review provides a pioneering perspective by integrating phytoremediation mechanisms with a comprehensive discussion of post-remediation biomass treatment methods, such as incineration, solidification, gasification, and pyrolysis, which are essential for reducing environmental risks. This study's output highlights that solidification is more suitable for radioactive biomass management for safe long-term storage and sustainable radioactive waste management; however, it does not produce value-added products. Meanwhile, gasification offers relatively low-emission biomass treatment compared to incineration and enables superior energy conversion efficiency and lower costs on a large scale compared to pyrolysis. The findings contribute to improving the overall efficiency of phytoremediation and provide insights into post-remediation biomass handling methods, reinforcing the feasibility of phytoremediation as a sustainable large-scale remediation solution. By identifying research gaps and proposing future directions to enhance the sustainability of phytoremediation, this review serves as an advantageous reference for policymakers, researchers, and environmental practitioners in designing effective phytoremediation strategies and post-remediation biomass management policies.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"381 ","pages":"Article 144475"},"PeriodicalIF":8.1,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ChemospherePub Date : 2025-05-15DOI: 10.1016/j.chemosphere.2025.144430
Juliana Picinini-Zambelli , Ana Letícia Hilário Garcia , Malu Siqueira Borges , Enaile Tuliczewski Serpa , Fernanda Rabaioli da Silva , Rafael Rodrigues Dihl , Juliana da Silva
{"title":"Exposure to emerging water contaminants and human health risk: Cytotoxic and genotoxic effects of caffeine and diethyltoluamide (DEET) on eukaryotic cells","authors":"Juliana Picinini-Zambelli , Ana Letícia Hilário Garcia , Malu Siqueira Borges , Enaile Tuliczewski Serpa , Fernanda Rabaioli da Silva , Rafael Rodrigues Dihl , Juliana da Silva","doi":"10.1016/j.chemosphere.2025.144430","DOIUrl":"10.1016/j.chemosphere.2025.144430","url":null,"abstract":"<div><div>The presence of emerging pollutants in aquatic ecosystems due to human activities poses substantial concerns. While many studies explore detection and removal techniques for these compounds, conventional treatment methods often fail to address emerging pollutants. Moreover, there is a lack of legislation defining safe thresholds for these substances in water. Consequently, caffeine and N,N-diethyl-<em>meta</em>-toluamide (DEET) persist in surface waters, including treated sources, with limited understanding of their genomic effects on human cells. This study aimed to assess the cytotoxic and genotoxic effects of caffeine and DEET, individually and in combination, at concentrations detected in drinking water, using HepG2 cells. Additionally, through systems biology, we sought to understand the underlying molecular mechanisms of both substances. Cytotoxicity was evaluated using MTT and Trypan Blue assays, while genotoxicity was assessed using the comet assay. The chemoproteomic interaction network was constructed using STITCH and STRING databases, with subnetworks analyzed using Cytoscape plugins (MCODE, CentiScaPe, and BiNGO). Both compounds reduced HepG2 cell viability in a dose-dependent manner in both assays. Caffeine and DEET also induced DNA damage at all tested concentrations, including in co-exposure. Proteins related to the inflammatory response, signaling pathways, and xenobiotic metabolism were the main hub-bottlenecks of the chemoproteomic interaction network. These findings underscore the urgent need for further investigations into the presence of emerging pollutants in drinking water and their potential risks to human health.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"381 ","pages":"Article 144430"},"PeriodicalIF":8.1,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ChemospherePub Date : 2025-05-15DOI: 10.1016/j.chemosphere.2025.144470
Lebea N. Nthunya , Alseno K. Mosai , Eduardo Alberto López-Maldonado , Mokgadi Bopape , Subhendu Dhibar , Yannick Nuapia , Timothy O. Ajiboye , Jamil A. Buledi , Amber R. Solangi , Syed Tufail H. Sherazi , Patrick N. Ndungu , Oranso T. Mahlangu , Bhekie B. Mamba
{"title":"Unseen threats in aquatic and terrestrial ecosystems: Nanoparticle persistence, transport and toxicity in natural environments","authors":"Lebea N. Nthunya , Alseno K. Mosai , Eduardo Alberto López-Maldonado , Mokgadi Bopape , Subhendu Dhibar , Yannick Nuapia , Timothy O. Ajiboye , Jamil A. Buledi , Amber R. Solangi , Syed Tufail H. Sherazi , Patrick N. Ndungu , Oranso T. Mahlangu , Bhekie B. Mamba","doi":"10.1016/j.chemosphere.2025.144470","DOIUrl":"10.1016/j.chemosphere.2025.144470","url":null,"abstract":"<div><div>Although nanoparticles (NPs) are increasingly used in various industries, their uncontrolled environmental release presents a potential risk to water bodies, vegetation and human health. Although previous review studies evaluated the toxicity and bioaccumulation of NPs, their long-term ecological impacts and transport dynamics in aquatic and terrestrial systems remain unexplored. The current review examined the mechanistic bioaccumulation, transport and environmental persistence of NPs, highlighting the need for concurrent risk assessment, regulation and management strategies. The multifaceted nature of nanotechnology necessitates a balanced approach considering both the benefits of NPs and their potential environmental and health risks, requiring comprehensive risk assessment and management strategies. The complexities of NPs risk assessment, emphasizing the unique properties of NPs influencing their toxicity and environmental behavior are critically addressed. Strategies to mitigate NPs' environmental impact include advanced monitoring techniques, regulatory frameworks tailored to NPs' unique properties, promotion of green nanotechnology practices, and NP remediation technologies. Given the complexity and uncertainty surrounding NPs, integration of regulatory, technological, and research-based strategies is imperative. This involves detailed NPs characterization techniques providing basic data for environmental fate prediction models and understanding of biologically relevant risk assessment models to safeguard our environment and public health. In this study, the recent advances in NPs persistence, environmental transport modelling and toxicity mechanisms are uniquely integrated, providing a framework to ecological risk assessment and regulatory approaches.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"382 ","pages":"Article 144470"},"PeriodicalIF":8.1,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ChemospherePub Date : 2025-05-15DOI: 10.1016/j.chemosphere.2025.144488
Zhiwei Cao , Zibin Pan , Zuliang Chen
{"title":"A comparative study on in-situ synthesis of two iron nanoparticles in acid mine drainage using green tea and Excoecaria cochinchinensis leaves extracts","authors":"Zhiwei Cao , Zibin Pan , Zuliang Chen","doi":"10.1016/j.chemosphere.2025.144488","DOIUrl":"10.1016/j.chemosphere.2025.144488","url":null,"abstract":"<div><div>Acid mine drainage (AMD) is a type of wastewater characterized by elevated concentrations of heavy metals and metalloids, posing significant threats to ecosystems and human health. This study presents an eco-friendly method for in-situ synthesis of iron nanoparticles (FeNPs) in AMD using Excoecaria cochinchinensis leaves (EC) and green tea extracts (GT) as reducing/stabilizing agents, enabling simultaneous removal of heavy metals and metalloids. Results indicate that the two FeNPs (EC-FeNPs and GT-FeNPs) produced in AMD exhibited distinct structural and reactive properties. Firstly, the removal efficiencies of EC-FeNPs for As, Ni and Cd were 20.4 %, 15.31 %, and 11.62 %, respectively, while those of GT-FeNPs were 10.07 %, 11.7 % and 19.76 %, respectively. This indicates that EC-FeNPs were generally more effective than GT-FeNPs in removing heavy metals and metalloids from AMD. Secondly, EC-FeNPs (170.14 ± 3.22 nm) displayed larger particle sizes and more stable surface charges compared to GT-FeNPs (127.14 ± 1.94 nm), which could be attributed to differences in functional groups in the respective plant extracts. Thirdly, both EC and GT extracts acted as reductants and stabilizers in the synthesis of two FeNPs, with EC extracts providing a larger amount of phenolic compounds and carboxylic acids than GT extracts. Highlighted in this study is a novel approach for resource recovery and simultaneous removal of heavy metals and metalloids from AMD.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"381 ","pages":"Article 144488"},"PeriodicalIF":8.1,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ChemospherePub Date : 2025-05-15DOI: 10.1016/j.chemosphere.2025.144469
Paulina Emanowicz , Paulina Średnicka , Michał Wójcicki , Joanna Kanabus , Marek Roszko , Edyta Juszczuk-Kubiak
{"title":"Evaluating the in vitro removal of bisphenol F and tetramethyl bisphenol F by Akkermansia muciniphila and Faecalibacterium prausnitzii","authors":"Paulina Emanowicz , Paulina Średnicka , Michał Wójcicki , Joanna Kanabus , Marek Roszko , Edyta Juszczuk-Kubiak","doi":"10.1016/j.chemosphere.2025.144469","DOIUrl":"10.1016/j.chemosphere.2025.144469","url":null,"abstract":"<div><div>We conducted pioneering research evaluating <em>Akkermansia muciniphila</em> and <em>Faecalibacterium prausnitzii</em> as next-generation probiotics (NGPs) for removing bisphenol A (BPA) analogues, such as bisphenol F (BPF) and tetramethylbisphenol F (TMBPF). Chronic exposure to these under-researched compounds through contaminated food poses a risk to health by promoting gut microbiota imbalances and inflammation. In our experiments, pasteurized <em>F. prausnitzii</em> removed up to ∼87 % of TMBPF after 48 h (from 9,976 ± 0,790 μg/mL to 1,350 ± 0,330 μg/mL μg/mL, p < 0.0001) through bioadsorption. Meanwhile, <em>A. muciniphila</em> achieved ∼48 % removal of BPF (from 10,33 ± 0,96 μg/mL to 5,33 ± 0,62 μg/mL, p < 0.0001) via biotransformation also after 48 h. Bioadsorption and biotransformation mechanisms were compared across conditions, with significant differences (p < 0.01) observed only for <em>A. muciniphila</em> with BPF and <em>F. prausnitzii</em> with TMBPF after 48 h. No cytotoxic effects of raw bisphenols were seen in Caco-2 cells, although the post-fermentation supernatant from <em>A. muciniphila</em> reduced cell viability to 68 % (p < 0.001). Notably, TMBPF showed no estrogenic activity, while BPF exhibited strong estrogenicity, which decreased following incubation with both strains. These findings confirm both NGPs reduce bisphenol concentrations, supporting their use in detoxification and functional food development. Implementing NGP-based strategies in food production and supplementation could lower human exposure to harmful bisphenols. This approach underscores the promise of advanced probiotics in mitigating foodborne chemical risks and aligns with evolving regulations and public health efforts to protect consumers.</div></div>","PeriodicalId":276,"journal":{"name":"Chemosphere","volume":"381 ","pages":"Article 144469"},"PeriodicalIF":8.1,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}