Chemistry and Physics of Lipids最新文献

{"title":"Synchrotron-based infrared microspectroscopy of polymeric nanoparticles and skin: Unveiling molecular interactions to enhance permeation","authors":"Sofia A. Costa Lima ,&nbsp;Ana Isabel Barbosa ,&nbsp;Cláudia Nunes ,&nbsp;Ibraheem Yousef ,&nbsp;Salette Reis","doi":"10.1016/j.chemphyslip.2022.105254","DOIUrl":"10.1016/j.chemphyslip.2022.105254","url":null,"abstract":"<div><p>The application of nanoparticles as permeation enhancers in skin drug delivery is a growing research field. However, the mechanisms of nanoparticles’ interaction with the skin structure are still unknown. Fucoidan/chitosan nanoparticles have demonstrated several physicochemical and biological advantages, among which is the enhancement of skin permeation. This study aims to elucidate permeation enhancement mechanisms using synchrotron-based Fourier Transform Infrared Microspectroscopy (SR-FTIRM) combined with multivariate analysis and in vitro skin permeation assay. Given the molecular weight influence on chitosan’s properties, the nanoparticles-skin interactions were evaluated with nanoparticles produced using low- and medium-molecular-weight chitosan. Chemical maps and spectral analysis revealed that fucoidan/chitosan nanoparticles induced changes in the lipids and protein regions. Inter-sample spectral differences were identified using principal component analysis. Low molecular weight fucoidan/ chitosan nanoparticles caused changes in the skin lipids’ lateral packing and structure at the stratum corneum layer towards a less ordered state and higher fluidity, and no evidence was found on proteins structure. The opposite was revealed for medium molecular weight fucoidan/chitosan nanoparticles, which induced changes in the secondary structure of keratin and altered lipid structure to an ordered and dense conformation. <em>In vitro</em> permeation assays with Franz diffusion cells correlate with the observed changes in the skin lipid and protein structure with enhanced skin permeation of a hydrophilic molecule incorporated within the fucoidan/chitosan nanoparticles. The findings of this study unveil molecular changes in the skin structure induced by the nanoparticles only possible with the application of the powerful and precise SR-FTIRM technique. This knowledge allows the design of nanoparticles towards an internalization pathway determining their fate within the skin structure.</p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"249 ","pages":"Article 105254"},"PeriodicalIF":3.4,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0009308422000822/pdfft?md5=8fb30dbf4a22432ddf497f6dcdd5a392&pid=1-s2.0-S0009308422000822-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10572713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of polyols on membrane structures of liposomes: A study using small-angle X-ray scattering data and generalized indirect Fourier transformation","authors":"Aika Sekine ,&nbsp;Taku Ogura ,&nbsp;Koji Tsuchiya ,&nbsp;Kaoru Ohishi ,&nbsp;Yuji Masubuchi ,&nbsp;Masaaki Akamatsu ,&nbsp;Kenichi Sakai ,&nbsp;Masahiko Abe ,&nbsp;Hideki Sakai","doi":"10.1016/j.chemphyslip.2022.105253","DOIUrl":"10.1016/j.chemphyslip.2022.105253","url":null,"abstract":"<div><p><span><span><span>This study aimed to evaluate the membrane structure<span> of distearoylphosphatidylcholine (DSPC) liposomes dispersed in water containing various types of polyols with low </span></span>molecular weight such as glycerin (Gly), 1,3-butandiol (BG), and </span>propylene<span> glycol (PG). To clarify the detailed membrane structure, generalized indirect Fourier transformation<span> (GIFT) analysis, which provides information about the bilayer spacing, bilayer thickness, number of lamellar layers, and membrane flexibility, was applied to small-angle X-ray scattering (SAXS) data of the present system. The GIFT results showed that the bilayer thickness of the DSPC liposomes followed the order Gly&gt;&gt;BG&gt;PG. In addition, the membrane flexibility estimated by the Caille parameter was in the order Gly&gt;&gt;BG&gt;PG; this result was supported by the gel-liquid crystal phase transition temperature (</span></span></span><em>T</em>_c<span>) obtained by differential scanning calorimetry<span> (DSC). These results, together with the Raman spectra, suggest that BG and PG incorporated into the bilayers of DSPC liposomes result in the formation of an interdigitated lamellar structure.</span></span></p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"249 ","pages":"Article 105253"},"PeriodicalIF":3.4,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10571528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colloidal delivery of vitamin E into solid lipid nanoparticles as a potential complement for the adverse effects of anemia treatment","authors":"Rocío C. Gambaro ,&nbsp;Ignacio Rivero Berti ,&nbsp;Maximiliano L. Cacicedo ,&nbsp;Stephan Gehring ,&nbsp;Vera A. Alvarez ,&nbsp;Guillermo R. Castro ,&nbsp;Analía Seoane ,&nbsp;Gisel Padula ,&nbsp;German A. Islan","doi":"10.1016/j.chemphyslip.2022.105252","DOIUrl":"10.1016/j.chemphyslip.2022.105252","url":null,"abstract":"<div><p><span>Vitamin E<span><span> (VitE) is one of the most important antioxidants and plays a key role in decreasing the inflammatory effects of </span>oxidative stress<span> caused by recurrent doses of iron administration in anemia treatment. However, VitE is poorly soluble in aqueous environments. Here, VitE encapsulation into solid lipid<span> nanoparticles (SLN) composed of myristil myristate to improve its bioavailability was proposed. A 99.9 ± 0.1% encapsulation efficiency with a drug/lipid ratio of 500 µg/mg and 478 higher VitE solubility was obtained. The antioxidant properties of VitE after encapsulation were maintained. SLN-VitE showed a 228.2 nm mean diameter with low polidispersitivity (0.335), and negative Z potential (ζ ≈ −9.0 mV). The SLN were well-dispersed, displayed spherical and homogeneous morphology by TEM. A controlled release of VitE from SLN was found. The XRD and </span></span></span></span>FTIR<span> analyses revealed the presence of a nanostructured architecture of SLN after VitE incorporation. We probed the safety of SLN-VitE after contact with three in vitro cell models: erythrocytes, lymphocytes and HepG2 cells. The cell viability<span> in presence of SLN, SLN-VitE, and their combinations with iron was not affected. The comet assay<span> demonstrated that the DNA damage caused by iron administration was decrease in presence of SLN-VitE.</span></span></span></p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"249 ","pages":"Article 105252"},"PeriodicalIF":3.4,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10626356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticancer drugs tamoxifen and 4hydroxytamoxifen as effectors of phosphatidylethanolamine lipid polymorphism","authors":"Julia Ortiz,&nbsp;José A. Teruel,&nbsp;Francisco J. Aranda,&nbsp;Antonio Ortiz","doi":"10.1016/j.chemphyslip.2022.105239","DOIUrl":"10.1016/j.chemphyslip.2022.105239","url":null,"abstract":"<div><p>The interaction of tamoxifen (TMX) and its metabolite 4-hydroxytamoxifen (HTMX) with a biomimetic membrane model system composed of 1,2-dielaidoylphosphatidylethanolamine (DEPE) has been studied using a biophysical approach. Incorporation of TMX into DEPE bilayers gives rise to a progressive broadening of the L_β/L_α phase transition and a downward temperature shift. The L_β/L_α phase transition presents multiple endotherms, indicating a lateral segregation of TMX/DEPE domains within the plane of the bilayer. TMX and HTMX also widen and shift the L_α to hexagonal-H_II transition toward lower values, the phase diagrams showing that both compounds facilitate formation of the H_II phase. TMX increases motional disorder of DEPE acyl chains in the L_β, L_α and H_II phases, whereas the effect of HTMX is clearly different. In addition, neither TMX nor HTMX significantly perturb the hydration state of the polar headgroup region of DEPE. Molecular dynamics (MD) simulations indicate that these drugs do not affect membrane thickness, area per lipid, or the conformation of DEPE molecules. As a general rule, the interaction of HTMX with DEPE is qualitatively similar to TMX but less intense. However, a significant difference shown by MD is that HTMX is mainly placed around the center of each monolayer while TMX is located mainly at the center of the membrane, also having a greater tendency to cluster formation. These results are discussed to understand the modulation of phosphatidylethanolamine lipid polymorphism carried out by these drugs, which could be of relevance to explain their effects on enzyme activity or membrane permeabilization.</p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"248 ","pages":"Article 105239"},"PeriodicalIF":3.4,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0009308422000676/pdfft?md5=b32b7b9d57f25ffc67630065c63ae3e9&pid=1-s2.0-S0009308422000676-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10571789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of 1-alkyl-1-methylpiperidinium bromides on lipids of fungal plasma membrane and lung surfactant","authors":"Katarzyna Dopierała,&nbsp;Anna Syguda,&nbsp;Marta Wojcieszak,&nbsp;Katarzyna Materna","doi":"10.1016/j.chemphyslip.2022.105240","DOIUrl":"10.1016/j.chemphyslip.2022.105240","url":null,"abstract":"<div><p><span><span>This study aimed to investigate the potential of 1-alkyl-1-methylpiperidinium bromides as fungicides and evaluate their impact on the human respiratory system when spread in the atmosphere. We investigated the behavior of </span>membrane lipids<span> and model membranes in the presence of a series of amphiphilic 1-alkyl-1-methylpiperidinium bromides ([MePipC</span></span>_n][Br]), differing in the alkyl chain length (n = 4 − 18). The experiments were performed with the Langmuir monolayer technique using 1,2-dipalmitoyl-<em>sn</em><span><span>-glycero-3-phosphocholine (DPPC) and ergosterol<span> (ERG)–the main components of lung surfactant and fungal plasma membrane, respectively and their mixtures with </span></span>phospholipids and sterols. The mixtures were chosen as the representatives of target and non−target organisms. The surface pressure−area isotherms were obtained by compressing monolayers in the presence of [MePipC</span>_n<span>][Br] in the subphase. The results were analyzed in terms of area expansion/contraction and compressibility<span>. The surface activity of the studied organic salts was also studied. In addition, the monolayers were deposited on a solid surface and their topography was investigated using atomic force microscopy<span>. This research implies that the studied compounds may destabilize efficiently the fungal plasma membrane. At the same time we demonstrated the significant impact of 1-alkyl-1-methylpiperidinium bromides on the lung surfactant layer. The interaction between [MePipCn][Br] and model membranes depends on the concentration and alkyl chain length of organic salt. The key role of contact time has been also revealed. The results may be helpful in the reasonable development of new agrochemical products aiming at the treatment of fungal infections in plants. In addition, our study indicates the significance of proper safety management while spreading the fungicides in the environment.</span></span></span></p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"248 ","pages":"Article 105240"},"PeriodicalIF":3.4,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10625814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Studies on the interactions of tiny amounts of common ionic surfactants with unsaturated phosphocholine lipid model membranes","authors":"Alicja Sęk ,&nbsp;Paulina Perczyk ,&nbsp;Aleksandra Szcześ ,&nbsp;Rainhard Machatschek ,&nbsp;Paweł Wydro","doi":"10.1016/j.chemphyslip.2022.105236","DOIUrl":"10.1016/j.chemphyslip.2022.105236","url":null,"abstract":"<div><p><span><span><span>In order to provide the fundamental information about the interactions of common anionic surfactants<span> with the basic unsaturated phospholipids the influence of three cationic (dodecyltrimethylammonium bromide, DTAB; tetradecyltrimethylammonium bromide, TTAB and hexadecyltrimethylamonium bromide, CTAB) and one anionic (sodium dodecylsulfate, SDS) surfactants on the properties of the 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) layers was investigated. The studies proved that a tiny amount of the </span></span>ionic surfactant<span> added to the already synthesized liposome suspension is sufficient to change the zeta potential<span> of the POPC and DOPC liposomes significantly. This impact increases with the surfactant concentration, the alkyl chain length of the surfactant and the degree of lipid saturation. Moreover, this effect is greater for the anionic surfactant than for the cationic one of the same alkyl chain length. The observed findings were confirmed in the course of the research carried out with the use of the corresponding Langmuir monolayers where the surface pressure – mean area isotherms, the </span></span></span>compressibility modulus – surface pressure dependences, the monolayer penetration tests, the surface potential – mean molecular area isotherms and Brewster angle </span>microscopy were discussed. It was found that the presence of the surfactants shifts the isotherms towards larger molecular area, to the higher extent for the SDS than DTAB. This effect increases with the increasing surfactant concentration in the subphase. Moreover, the investigated surfactants remain in the monolayer even at high surface pressure. Nevertheless, no effect on the morphology of the POPC and DOPC monolayers was detected from the BAM images. The surface potential and surface charge of the liposomes calculated on the basis of the zeta potential results reflected the interactions between the surfactant and the lipid layers.</p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"248 ","pages":"Article 105236"},"PeriodicalIF":3.4,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10571361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication, modification and application of lipid nanotubes","authors":"Hongmei Bi ,&nbsp;Zeqin Chen ,&nbsp;Liuchun Guo ,&nbsp;Yingmei Zhang ,&nbsp;Xinru Zeng ,&nbsp;Liuyi Xu","doi":"10.1016/j.chemphyslip.2022.105242","DOIUrl":"10.1016/j.chemphyslip.2022.105242","url":null,"abstract":"<div><p>The potential application of high aspect-ratio nanomaterials<span> motivates the development of the fabrication and modification of lipid nanotubes(LNTs). To date, diverse fabricate processes and elaborate template procedures have produced suitable tubular architectures with definite dimensions and complex structures for expected functions and applications. Herein, we comprehensively summarize the fabrication of LNTs in vitro and discuss the progress made on the micro/nanomaterials fabrication using LNTs as a template, as well as the functions and possible application of a wide range of LNTs as fundamental or derivative material. In addition, the characteristics, advantages, and disadvantages of different fabrication, modification methods, and development prospects of LNTs were briefly summarized.</span></p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"248 ","pages":"Article 105242"},"PeriodicalIF":3.4,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10575456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipidomic analysis reveals the effect of passive smoking on facial skin surface lipid in females","authors":"Mengzhen Zhao ,&nbsp;Keliang Fan ,&nbsp;Jia Wang ,&nbsp;Juan Wang ,&nbsp;Qingqing Xu ,&nbsp;Dandan Wei ,&nbsp;Yuanyuan Chen ,&nbsp;Lihong Zhou ,&nbsp;Zhenxing Mao ,&nbsp;Tian Chen","doi":"10.1016/j.chemphyslip.2022.105228","DOIUrl":"10.1016/j.chemphyslip.2022.105228","url":null,"abstract":"<div><h3>Background</h3><p>Smoking has toxic effects on the skin and can damage it. However, few studies have focused on the lipid profile<span> changes of facial skin surface lipids (SSL) by passive smoking.</span></p></div><div><h3>Method</h3><p><span>A cross-sectional analytical study was conducted on middle-aged females volunteered from Henan, China to participate in the study. A total of 20 passive smoking females and 20 non-passive smoking females were recruited for this study. The components of skin surface lipids were measured by ultra-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-QTOF-MS). </span>Multivariate data analysis and enrichment analysis were used to investigate the differences in facial SSL between passive and non-passive smoking females.</p></div><div><h3>Result</h3><p>There were 1247 lipid<span><span> entities identified in facial SSL between passive and non-passive smoking females. Significant differences in composition of facial SSL were observed between the two groups. After multivariate data analysis suggested, 28 significantly different lipids were identified and classified into four classes in SSL of the female cheeks. As well as 32 significantly different lipids were obtained in SSL of the female foreheads, which included three classes of lipids. Subsequent analysis revealed that the content of fatty acids (FA) in passive smoking females was significantly reduced and the content of </span>glycerolipids<span> (GL) and sphingolipids (SP) increased, compared with the control group.</span></span></p></div><div><h3>Conclusion</h3><p><span>These results indicated that an increase in GLs and SPs of facial lipids and a decrease in FAs in passive smoking females. These changes in lipids might be associated with oxidative stress and interference with </span>signaling pathways by substances in smoke. And passive smoking affected facial SSL and changed the content and metabolism of skin lipids.</p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"247 ","pages":"Article 105228"},"PeriodicalIF":3.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40592654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel and easy to prepare azo-based bioreductive linker and its application in hypoxia-sensitive cationic liposomal doxorubicin: Synthesis, characterization, in vitro and in vivo studies in mice bearing C26 tumor","authors":"Mohammad Mashreghi ,&nbsp;Mahdi Faal Maleki ,&nbsp;Anis Askarizadeh ,&nbsp;Helaleh Farshchi ,&nbsp;Leila Farhoudi ,&nbsp;Mahda Sadat Nasrollahzadeh ,&nbsp;Mahere Rezazade Bazaz ,&nbsp;Farzin Hadizadeh ,&nbsp;Mahmoud Reza Jaafari","doi":"10.1016/j.chemphyslip.2022.105226","DOIUrl":"10.1016/j.chemphyslip.2022.105226","url":null,"abstract":"<div><p><span>This study designed and synthesized a cost-effective azo-based hypoxia-sensitive linker (AHSL) using commercially accessible, inexpensive raw materials and simple methods to apply in cationic nanoliposomes<span><span>. Then, AHSL was post-inserted into the cationic liposome (Cat-lip), and PEG-Azo-Cat-lip was prepared and characterized using DLS. The decrease in the zeta-potential of formulation from + 18.4 mV for Cat-lip to + 6.1 mV and the increase in the size of the PEG-Azo-Cat-lip indicated the successful post insertion of AHSL into the liposomes. The Doxorubicin (Dox) release study showed that </span>PEGylation results in a more stable PEG-Azo-Cat-lip than the Cat-lip. The increased cytotoxicity of the PEG-Azo-Cat-lip in the hypoxic condition also indicated the cleavage of the AHSL in the hypoxic environment. </span></span><em>In vivo</em><span> biodistribution<span> using animal imaging has shown higher tumor accumulation of the MPEG-Azo-Cat-lip than Cat-lip during the 120 h of the study. The results of anti-tumor activities and biosafety of the formulations also showed the higher efficiency of the MPEG-Azo-Cat-lip compared with the Cat-lip. The results of this study indicated the antitumor efficacy of this hypoxia-sensitive which merits further investigation.</span></span></p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"247 ","pages":"Article 105226"},"PeriodicalIF":3.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40517093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of aerobic exercise on the lipophagy of adipose tissue in obese male mice","authors":"Rendong Li ,&nbsp;Guangkuan Li ,&nbsp;Yan Hai ,&nbsp;Tao Li ,&nbsp;Yuanyuan Bian ,&nbsp;Tie Ma","doi":"10.1016/j.chemphyslip.2022.105225","DOIUrl":"https://doi.org/10.1016/j.chemphyslip.2022.105225","url":null,"abstract":"<div><p><span><span><span>This article explores the obesity state and the changes in the level of lipophagy in adipose tissue after exercise to lose weight, so as to provide direction and basis for theoretical research on obesity prevention and control. We established a high-fat diet model of </span>obese mice<span>, and applied exercise intervention and intraperitoneal injection of </span></span>chloroquine<span> to inhibit autophagy. Long-term high-fat diet can cause obesity in mice, and the process of lipophagy is inhibited, which may be one of the reasons for fat accumulation. Eight weeks of aerobic exercise can effectively reduce the weight of obese mice and promote </span></span>lipolysis<span>; this process is mainly completed by lipase decomposition, in addition to require the participation of the lipophagy process.</span></p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"247 ","pages":"Article 105225"},"PeriodicalIF":3.4,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"1554530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}