Chemistry and Physics of Lipids最新文献

{"title":"The impact of phospholipids with high transition temperature to enhance redox-sensitive liposomal doxorubicin efficacy in colon carcinoma model","authors":"Elaheh Mirhadi ,&nbsp;Anis Askarizadeh ,&nbsp;Leila Farhoudi ,&nbsp;Mohammad Mashreghi ,&nbsp;Saeed Behboodifar ,&nbsp;Seyedeh Hoda Alavizadeh ,&nbsp;Leila Arabi ,&nbsp;Mahmoud Reza Jaafari","doi":"10.1016/j.chemphyslip.2024.105396","DOIUrl":"https://doi.org/10.1016/j.chemphyslip.2024.105396","url":null,"abstract":"<div><p>In this study, we have developed a redox-sensitive (RS) liposomal doxorubicin formulation by incorporating 10,10′-diselanediylbis decanoic acid (DDA) organoselenium compound as the RS moiety. Hence, several RS liposomal formulations were prepared by using DOPE, HSPC, DDA, mPEG₂₀₀₀-DSPE, and cholesterol. <em>In situ</em> drug loading using a pH gradient and citrate complex yielded high drug to lipid ratio and encapsulation efficiency (100<!--> <!-->%) for RS liposomes. Liposomal formulations were characterized in terms of size, surface charge and morphology, drug loading, release properties, cell uptake and cytotoxicity, as well as therapeutic efficacy in BALB/c mice bearing C26 tumor cells. The formulations showed an average particle size of 200 nm with narrow size distributions (PDI &lt; 0.3), and negative surface charges varying from −6 mV to −18.6 mV. Our study confirms that the presence of the DDA compound in liposomes is highly sensitive to hydrogen peroxide at 0.1<!--> <!-->% w/v, resulting in a significant burst release of up to 40<!--> <!-->%. The <em>in vivo</em> therapeutic efficacy study in BALB/c mice bearing C26 colon carcinoma confirmed the promising function of RS liposomes in the tumor microenvironment which led to a prolonged median survival time (MST). The addition of hydrogenated soy phosphatidylcholine (HSPC) with a high transition temperature (Tm: 52–53.5<!--> <!-->°C) extended the MST of our 3-component formulation of F14 (DOPE/HSPC/DDA) to 60 days in comparison to Caelyx (PEGylated liposomal Dox), which is not RS-sensitive (39 days). Overall, HSPC liposomes bearing RS-sensitive moiety enhanced therapeutic efficacy against colon cancer <em>in vitro</em> and <em>in vivo</em>. This achievement unequivocally underscores the criticality of high-TM phospholipids, particularly HSPC, in significantly enhancing liposome stability within the bloodstream. In addition, RS liposomes enable the on-demand release of drugs, leveraging the redox environment of tumor cells, thereby augmenting the efficacy of the formulation.</p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"261 ","pages":"Article 105396"},"PeriodicalIF":3.4,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140644349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-lamellar lyotropic liquid crystalline nanoparticles as nanocarriers for enhanced drug encapsulation of atorvastatin calcium and proanthocyanidins","authors":"Mardhiah Maslizan ,&nbsp;Muhammad Salahuddin Haris ,&nbsp;Mokrish Ajat ,&nbsp;Siti Nurul Ain Md Jamil ,&nbsp;Shah Christirani Azhar ,&nbsp;N. Idayu Zahid ,&nbsp;Intan Diana Mat Azmi","doi":"10.1016/j.chemphyslip.2024.105377","DOIUrl":"10.1016/j.chemphyslip.2024.105377","url":null,"abstract":"<div><p>Atorvastatin calcium (ATV) and proanthocyanidins (PAC) have a strong antioxidant activity, that can benefit to reduce the atherosclerotic plaque progression. Unfortunately, the bioavailability of ATV is greatly reduced due to its limited drug solubility while the PAC drug is unstable upon exposure to the atmospheric oxygen. Herein, the lyotropic liquid crystalline nanoparticles (LLCNPs) constructed by a binary mixture of soy phosphatidylcholine (SPC) and citric acid ester of monoglyceride (citrem) at different weight ratios were used to encapsulate the hydrophobic ATV and hydrophilic PAC. The LLCNPs were further characterized by small-angle X-ray scattering and dynamic light scattering. Depending on the lipid composition, the systems have a size range of 140–190 nm and were able to encapsulate both drugs in the range of 90–100%. Upon increasing the citrem content of drug-loaded LLCNPs, the hexosomes (H₂) was completely transformed to an emulsified inverse micellar (L₂). The optimum encapsulation efficiency (EE) of ATV and PAC were obtained in citrem/SPC weight ratio 4:1 (L₂) and 1:1 (H₂), respectively. There was a substantial change in the mean size and PDI of the nanoparticles upon 30 days of storage with the ATV-loaded LLCNPs exhibiting greater colloidal instability than PAC-loaded LLCNPs. The biphasic released pattern (burst released at the initial stage followed by the sustained released at the later stage) was perceived in ATV formulation, while the burst drug released pattern was observed in PAC formulations that could be attributed by its internal H₂ structure. Interestingly, the cytokine studies showed that the PAC-LLCNPs promisingly up regulate the expressions of tumor necrosis factor-alpha (TNF-α) better than the drug-free and ATV-loaded LLCNPs samples. The structural tunability of citrem/SPC nanoparticles and their effect on physicochemical characteristic, biological activities and potential as an alternative drug delivery platform in the treatment of atherosclerosis are discussed.</p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"260 ","pages":"Article 105377"},"PeriodicalIF":3.4,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139688629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bottom-up approach to explore alpha-amylase assisted membrane remodelling","authors":"Harshit Kumar ,&nbsp;Sayar Mandal ,&nbsp;Reena Yadav ,&nbsp;Suhasi Gupta ,&nbsp;Hemraj Meena ,&nbsp;Mayur Kadu ,&nbsp;Rajni Kudawla ,&nbsp;Pratibha Sharma ,&nbsp;Indu Pal Kaur ,&nbsp;Subhabrata Maiti ,&nbsp;John H. Ipsen ,&nbsp;Tripta Bhatia","doi":"10.1016/j.chemphyslip.2023.105374","DOIUrl":"10.1016/j.chemphyslip.2023.105374","url":null,"abstract":"<div><p><span>Soluble alpha-amylases play an important role in the catabolism of polysaccharides. In this work, we show that the malt </span><em>α</em><span><span><span> -amylase can interact with the lipid membrane<span> and further alter its mechanical properties. Vesicle fluctuation spectroscopy is used for quantitative measurement<span> of the membrane bending rigidity of phosphatidylcholines </span></span></span>lipid vesicles from the shape fluctuation based on the whole contour of Giant </span>Unilamellar Vesicles<span> (GUVs). The bending rigidity of the 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine lipid vesicles in water increases significantly with the presence of 0.14 micromolar alpha-amylase (AA) in the exterior solution. It appears that the enzyme<span> present in the external solution interacts with the outer layer of the bilayer membrane, leading to an asymmetry of the solution on either side of the bilayer membrane and altering its elasticity. At AA concentration of 1.5 micromolars and above, changes in the morphology of the GUV membrane are observed. The interaction between AA in the external solution and the external leaflet causes the bilayer membrane to curve spontaneously, leading to the formation of outbuds, giving a positive spontaneous curvature of </span></span></span><em>C</em>₀ ≤ 0.05 <em>μ</em>m⁻¹<span> at ≈ 1 mg / ml of the AA concentration. We validate and characterize its concentration-dependent role in stabilizing the membrane curvature. Our findings indicate that the involvement of the enzyme, depending on the concentration, can have a considerable effect on the mechanical characteristics of the membrane.</span></p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"259 ","pages":"Article 105374"},"PeriodicalIF":3.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139095368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of glucose and fructose on lipid droplet metabolism in human normal bronchial and cancer lung cells by Raman spectroscopy","authors":"Monika Kopec,&nbsp;Karolina Beton-Mysur","doi":"10.1016/j.chemphyslip.2023.105375","DOIUrl":"10.1016/j.chemphyslip.2023.105375","url":null,"abstract":"<div><p>Fructose is one of the most important monosaccharides in the human diet that the human body needs for proper metabolism. This paper presents an approach to study biochemical changes caused by sugars in human normal bronchial cells (BEpiC) and human cancer lung cells (A549) by Raman spectroscopy and Raman imaging. Results after supplementation of human bronchial and lung cells with fructose are also discussed and compared with results obtained for pure human bronchial and lung cells. Based on Raman techniques we have proved that peaks at 750 cm⁻¹, 1126 cm⁻¹, 1444 cm⁻¹, 1584 cm⁻¹ and 2845 cm⁻¹ can be treated as biomarkers to monitor fructose changes in cells. Results for fructose have been compared with results for glucose. Raman analysis of the bands at 750 cm⁻¹, 1126 cm⁻¹, 1584 cm⁻¹ and 2845 cm⁻¹ for pure BEpiC and A549 cells and BEpiC and A549 after supplementation with fructose and glucose are higher after supplementation with fructose in comparison to glucose. The obtained results shed light on the uninvestigated influence of glucose and fructose on lipid droplet metabolism by Raman spectroscopy methods.</p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"259 ","pages":"Article 105375"},"PeriodicalIF":3.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S000930842300097X/pdfft?md5=665ff1b34c9da1f8e4adcc3bcf6d4f0f&pid=1-s2.0-S000930842300097X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139072262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Closer look at the calorimetric lower transition in lipid bilayers","authors":"Sophia A. Korono,&nbsp;John F. Nagle","doi":"10.1016/j.chemphyslip.2023.105366","DOIUrl":"10.1016/j.chemphyslip.2023.105366","url":null,"abstract":"<div><p>The thermal behavior of unilamellar vesicles has been revisited with differential scanning calorimetry to address the issue of whether it is essential to include interactions between neighboring bilayers in theories and simulations of the ripple phase. The issue focuses on the lower, <em>aka</em> pretransition, and the ripple phase that clearly exists between the lower and main transitions in multilamellar vesicles (MLV). We find anomalous thermal behavior in unilamellar vesicles (ULV) beginning at the same temperature as the lower transition in MLVs, but this feature is considerably broadened and somewhat weaker compared to the lower transition in MLVs. We ascribe this to the difficulty of packing a regular ripple pattern on small spheres. In agreement with a few reports of a ripple phase in direct images of single bilayers, we conclude that interactions between neighboring bilayers are not essential for the ripple phase in lipid bilayers.</p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"259 ","pages":"Article 105366"},"PeriodicalIF":3.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0009308423000889/pdfft?md5=40ff883edcd280818ebff926ab6c6002&pid=1-s2.0-S0009308423000889-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138561661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Micelle formation of sodium taurolithocholate","authors":"Keisuke Matsuoka ,&nbsp;Rina Sekiguchi ,&nbsp;Tomokazu Yoshimura","doi":"10.1016/j.chemphyslip.2024.105378","DOIUrl":"10.1016/j.chemphyslip.2024.105378","url":null,"abstract":"<div><p>The proportion of sodium taurolithocholate (NaTLC) is extremely low in human bile salts. NaTLC forms aggregates with other lipids in the bile and functions as an emulsifying and solubilizing agent. The molecular structure of NaTLC contains hydrophilic hydroxyl and sulfonic acid groups at both ends of the steroid ring. This molecular structure is similar to bolaform amphiphilic substance having hydrophilic groups at both ends due to the characteristics of its molecular structure. This study investigated the aggregate properties of the NaTLC using surface tension measurements, light scattering, small-angle X-ray scattering (SAXS), and cryo-transmission electron microscopy (cryo-TEM). Surface tension measurement showed that the surface tension of the NaTLC solution decreased to 54 mN m⁻¹. The concentration that showed the minimum surface tension corresponded to the critical micelle concentration (CMC: 0.6 mmol L⁻¹, 308 K) determined by the change in light scattering intensity. On the other hand, the degree of counterion (sodium ions) binding to the micelles increased with increasing NaTLC concentration. SAXS and cryo-TEM measurements showed that the NaTLC formed large string-like micelles. The surface activity and large aggregates showed the potential for use as biosurfactants. However, because of the relatively low solubility of NaTLC in water, its use as a biosurfactant is limited to a narrow concentration range.</p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"259 ","pages":"Article 105378"},"PeriodicalIF":3.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139688531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of structural effects of cholesterol, lanosterol, and oxysterol on phospholipid (POPC) bilayers","authors":"Ayumi Okayama,&nbsp;Tatsuya Hoshino,&nbsp;Kohei Wada,&nbsp;Hiroshi Takahashi","doi":"10.1016/j.chemphyslip.2024.105376","DOIUrl":"10.1016/j.chemphyslip.2024.105376","url":null,"abstract":"<div><p>Membrane sterols contribute to the function of biomembranes by regulating the physical properties of the lipid bilayers. Cholesterol, a typical mammalian sterol, is biosynthesized by oxidation of lanosterol. From a molecular evolutionary perspective, lanosterol is considered the ancestral molecule of cholesterol. Here, we studied whether cholesterol is superior to lanosterol in regulating the physical properties of the lipid bilayer in terms of the structural effect on model biomembranes composed of a phospholipid. For comparison, oxysterol, which is formed by oxidation of cholesterol, was also studied. The phospholipid used was 1-palmitoyl-2-oleoyl-<em>sn</em>-glycero-3-phosphocholine (POPC), which is abundantly found in mammalian biomembranes, and 7β-hydroxycholesterol, which is highly cytotoxic, was used as the oxysterol. The apparent molecular volume was calculated from the mass density determined by the flotation method using H₂O and D₂O, and the bilayer thickness was determined by reconstructing the electron density distribution from X-ray diffraction data of the POPC/sterol mixtures at a sterol concentration of 30 mol%. The apparent occupied area at the bilayer surface was calculated from the above two structural data. The cholesterol system had the thickest bilayer thickness and the smallest occupied area of the three sterols studied here. This indicates that the POPC/cholesterol bilayer has a better barrier property than the other two systems. Compared to cholesterol, the effects of lanosterol and 7β-hydroxycholesterol on lipid bilayer properties can be interpreted as suboptimal for the function of mammalian biomembranes.</p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"259 ","pages":"Article 105376"},"PeriodicalIF":3.4,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139688534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural changes in layers of lipid mixtures at low surface tensions","authors":"A.G. Bykov ,&nbsp;M.A. Panaeva ,&nbsp;O.Y. Milyaeva ,&nbsp;A.V. Michailov ,&nbsp;A.R. Rafikova ,&nbsp;E. Guzman ,&nbsp;R. Rubio ,&nbsp;R. Miller ,&nbsp;B.A. Noskov","doi":"10.1016/j.chemphyslip.2023.105365","DOIUrl":"10.1016/j.chemphyslip.2023.105365","url":null,"abstract":"<div><p>Layers of pulmonary lipids on an aqueous substrate at non-equilibrium conditions can decrease the surface tension of water to quite low values. This is connected with different relaxation processes occurring at the interface and the associated changes in the surface layer structure. Results of measurements by the combination of methods like surface rheology, ellipsometry, Brewster angle microscopy, and IRRAS for spread layers of lipid mixtures open a possibility to specify the dynamics of structural changes at conditions close to the physiological state. At sufficiently low surface tension values (below 5 mN/m) significant changes in the ellipsometric signal were observed for pure DPPC layers, which can be related to a transition from 2D to 3D structures caused by the layer folding. The addition of other lipids can accelerate the relaxation processes connected with squeezing-out of molecules or multilayer stacks formation hampering thereby a decrease of surface tension down to low values corresponding to the folding of the monolayer.</p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"258 ","pages":"Article 105365"},"PeriodicalIF":3.4,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0009308423000877/pdfft?md5=c6ea824731de0addde49f7c4c3e09c51&pid=1-s2.0-S0009308423000877-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138687102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interactions between macrophage membrane and lipid mediators during cardiovascular diseases with the implications of scavenger receptors","authors":"Sangeetha Ravi ,&nbsp;Livya Catherene Martin ,&nbsp;Mahalakshmi Krishnan ,&nbsp;Manikandan Kumaresan ,&nbsp;Beulaja Manikandan ,&nbsp;Manikandan Ramar","doi":"10.1016/j.chemphyslip.2023.105362","DOIUrl":"10.1016/j.chemphyslip.2023.105362","url":null,"abstract":"<div><p>The onset and progression of cardiovascular diseases with the major underlying cause being atherosclerosis, occur during chronic inflammatory persistence in the vascular system, especially within the arterial wall. Such prolonged maladaptive inflammation is driven by macrophages and their key mediators are generally attributed to a disparity in lipid metabolism. Macrophages are the primary cells of innate immunity, endowed with expansive membrane domains involved in immune responses with their signalling systems. During atherosclerosis, the membrane domains and receptors control various active organisations of macrophages. Their scavenger/endocytic receptors regulate the trafficking of intracellular and extracellular cargo. Corresponding influence on lipid metabolism is mediated by their dynamic interaction with scavenger membrane receptors and their integrated mechanisms such as pinocytosis, phagocytosis, cholesterol export/import, etc. This interaction not only results in the functional differentiation of macrophages but also modifies their structural configurations. Here, we reviewed the association of macrophage membrane biomechanics and their scavenger receptor families with lipid metabolites during the event of atherogenesis. In addition, the membrane structure of macrophages and the signalling pathways involved in endocytosis integrated with lipid metabolism are detailed. This article establishes future insights into the scavenger receptors as potential targets for cardiovascular disease prevention and treatment.</p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"258 ","pages":"Article 105362"},"PeriodicalIF":3.4,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0009308423000841/pdfft?md5=17934960823409dc7f16ffeb9760fdd9&pid=1-s2.0-S0009308423000841-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138439974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of cationic dendrimer on membrane mimetic systems in the form of monolayer and bilayer","authors":"Biplab Roy ,&nbsp;Pritam Guha ,&nbsp;Chien-Hsiang Chang ,&nbsp;Prasant Nahak ,&nbsp;Gourab Karmakar ,&nbsp;Alexey G. Bykov ,&nbsp;Alexander V. Akentiev ,&nbsp;Boris A. Noskov ,&nbsp;Anuttam Patra ,&nbsp;Kunal Dutta ,&nbsp;Chandradipa Ghosh ,&nbsp;Amiya Kumar Panda","doi":"10.1016/j.chemphyslip.2023.105364","DOIUrl":"10.1016/j.chemphyslip.2023.105364","url":null,"abstract":"<div><p>Interactions between a zwitterionic phospholipid, 1, 2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) and four anionic phospholipids dihexadecyl phosphate (DHP), 1, 2-dimyristoyl-sn-glycero-3-phosphoglycerol (DMPG), 1, 2-dipalmitoyl-sn-glycero-3-phosphate (DPP) and 1, 2-dipalmitoyl-sn-glycero-3-phospho ethanol (DPPEth) in combination with an additional amount of 30 mol% cholesterol were separately investigated at air-buffer interface through surface pressure (π) - area (A) measurements. π-A isotherm derived parameters revealed maximum negative deviation from ideality for the mixtures comprising 30 mol% anionic lipids. Besides the film functionality, structural changes of the monomolecular films at different surface pressures in the absence and presence of polyamidoamine (PAMAM, generation 4), a cationic dendrimer, were visualised through Brewster angle microscopy and fluorescence microscopic studies. Fluidity/rigidity of monolayers were assessed by surface dilatational rheology studies. Effect of PAMAM on the formation of adsorbed monolayer, due to bilayer disintegration of liposomes (DPPC:anionic lipids= 7:3 M/M, and 30 mol% cholesterol) were monitored by surface pressure (π) - time (t) isotherms. Bilayer disintegration kinetics were dependent on lipid head group and chain length, besides dendrimer concentration. Such studies are considered to be an in vitro cell membrane model where the alteration of molecular orientation play important roles in understanding the nature of interaction between the dendrimer and cell membrane. Liposome-dendrimer aggregates were nontoxic to breast cancer cell line as well as in doxorubicin treated MDA-MB-468 cell line suggesting their potential as drug delivery systems.</p></div>","PeriodicalId":275,"journal":{"name":"Chemistry and Physics of Lipids","volume":"258 ","pages":"Article 105364"},"PeriodicalIF":3.4,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0009308423000865/pdfft?md5=c4c51323e058927cf3eee1a594e7134f&pid=1-s2.0-S0009308423000865-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138469583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}