World Congress of the International Photodynamic Association最新文献_第5页

Effect of 5-aminolevulinic acid on the haem biosynthesis pathway in pancreatic cancer and pancreatic ductal epithelial cell lines 5-氨基乙酰丙酸对胰腺癌和胰腺导管上皮细胞系血红素生物合成途径的影响

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2525085

P. Labib, W. Al-Akkad, B. Davidson, A. MacRobert, S. Pereira

{"title":"Effect of 5-aminolevulinic acid on the haem biosynthesis pathway in pancreatic cancer and pancreatic ductal epithelial cell lines","authors":"P. Labib, W. Al-Akkad, B. Davidson, A. MacRobert, S. Pereira","doi":"10.1117/12.2525085","DOIUrl":"https://doi.org/10.1117/12.2525085","url":null,"abstract":"Introduction: 5-aminolevulinic acid (ALA) generates protoporphyrin IX (PpIX)-induced fluorescence by acting as a substrate for the haem biosynthesis pathway. Despite suggestions that ALA could be used for pancreatic cancer photodiagnostics, the pancreatic cancer cell line PANC-1 only shows weak fluorescence following ALA administration. A possible explanation was that the haem biosynthesis pathway varies between cancers. Methods: We compared the mRNA expression of the haem biosynthesis pathway of PANC-1 (weak fluorescence) with the pancreatic cancer cell line CFPAC-1 (strong fluorescence) and the pancreatic ductal cell line H6c7 (control) with or without 24 hours ALA incubation. Cells were seeded on day one, fresh media with or without ALA (0.5mM) added on day two, and RNA extracted on day three. Quantitative real-time polymerase chain reaction was performed to assess the relative mRNA expression of four membrane transporters and eight enzymes responsible for haem biosynthesis. Results: Post-ALA incubation, CFPAC-1 demonstrated significant downregulation of cell membrane ALA influx transporter PEPT1, downregulation of ALA synthase and upregulation of the mitochondrial membrane transporter ABCB6. PANC-1, whilst showing similar changes to ALA synthase and ABCB6, showed significant upregulation of the PpIX efflux transporter ABCG2. PANC-1 also had minimal PEPT1 expression pre- and post-ALA. H6c7 demonstrated significant up- or downregulation of three transporters and five enzymes. Conclusion: Poor PpIX-induced fluorescence in PANC-1 is likely to be secondary to decreased ALA influx from low PEPT1 expression and increased ABCG2 expression. The use of nanocarriers to deliver ALA and/or ABCG2 inhibitors may improve ALA-induced fluorescence in PANC-1 and other ALA-resistant cancers.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124858530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mild hyperthermia enhances drug accumulation and photodynamic therapy efficacy 轻度热疗可提高药物积累和光动力治疗效果

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2526182

S. Jenkins, Klressa Barnes, G. Shafirstein, R. Griffin

{"title":"Mild hyperthermia enhances drug accumulation and photodynamic therapy efficacy","authors":"S. Jenkins, Klressa Barnes, G. Shafirstein, R. Griffin","doi":"10.1117/12.2526182","DOIUrl":"https://doi.org/10.1117/12.2526182","url":null,"abstract":"Photodynamic therapy (PDT) of tumors relies on the delivery of a photosensitizer to the tumor site followed by external laser activation. The effectiveness of the therapy is determined by the fluence and irradiation of the laser, intratumoral photosensitizer retention, and availability of molecular oxygen. We hypothesized that retention and resulting therapeutic response may be improved with local hyperthermia. Tumors were grown in the rear limb then locally heated for 1 hour at 42.5 °C immediately following i.v. injection of the photosensitizer in an immunocompetent murine model. Laser exposure was applied at 2 h or 24 h after the heating session. Administration of heating caused a significant growth delay when a single laser treatment was applied only 2 hours following injection. However, in animals given photosensitizer only and irradiated 2 h later there was no measurable anti-tumor effect observed. The marked anti-tumor effects obtained with prior local hyperthermia were observed even as the dose was lowered from 10 mg/kg to 1.3 mg/kg. Additionally, histological analysis of our intial studies revealed that the majority of the tumor tissue (~75%) was necrotic after two days when heat was combined with PDT, while PDT alone resulted in only ~25% necrotic tissue. Additionally, a significant, though less notable, increase in the efficacy was observed if the laser treatment was applied 24 hours after hyperthermia and photosensitizer administration. This increase is in part ascribed to the increased retention of the photosensitizer in the tumor tissue and likely lasting effects on tumor blood flow and oxygenation in the heated vs. control groups.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114403255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Verteporfin Photodynamic therapy with 5 aza-deoxy-cytidine for neo-adjuvant treatment of primary breast cancer: Results of pre-clinical investigations 5氮杂脱氧胞苷光动力疗法对原发性乳腺癌的新辅助治疗:临床前研究结果

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2525337

S. Banerjee, P. Acedo, S. E. El Sheikh, A. Meecham, N. Williams, G. Gerrard, R. Hamoudi, A. Macrobert, M. Keshtgar

引用次数: 0

Influence of cationic, anionic or non-charged substituents on photodynamic activity of water-soluble zinc (aza)phthalocyanines 阳离子、阴离子或不带电取代基对水溶性锌（氮杂）酞菁光动力活性的影响

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2525591

M. Macháček, J. Kollár, M. Halašková, Monika Steklá, S. Makhseed, T. Šimůnek, Petr Zimcik

引用次数: 0

A severe inflammatory acne was successfully cleared by topical photodynamic therapy mediated with 5-aminolevulinic acid in an refractory aplastic anemia female patient: a case report 在难治性再生障碍性贫血女性患者中，5-氨基乙酰丙酸局部光动力疗法成功清除了严重的炎性痤疮:一例报告

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2525517

Jing-Yi Huang, F. Peng, Xiaojing Yang, Zhou Chen

引用次数: 0

5-Aminolevulinic acid photodynamic therapy: an effective treatment modality for female precancerous lesions: experience of a single center 5-氨基乙酰丙酸光动力疗法:女性癌前病变的有效治疗方式:单一中心的经验

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2525244

Guangzhi Liu, Xiaoli Lu, Liyun Yang, Guiqin Chen, L. Cheng, B. Mao, Ziyi Zhao, Qiuyun Yang

引用次数: 1

The role of death pathways in the efficacy of photokilling 死亡途径在光杀伤效果中的作用

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2524638

D. Kessel, W. Cho, H.-R. C. Kim

引用次数: 0

Curcumin-based photosensitization: a novel and green technology to decontaminate food systems 基于姜黄素的光敏化:一种新型的绿色食品净化技术

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2530661

Maral Seidi Damyeh, R. Mereddy, M. Netzel, Y. Sultanbawa

{"title":"Curcumin-based photosensitization: a novel and green technology to decontaminate food systems","authors":"Maral Seidi Damyeh, R. Mereddy, M. Netzel, Y. Sultanbawa","doi":"10.1117/12.2530661","DOIUrl":"https://doi.org/10.1117/12.2530661","url":null,"abstract":"Different processing technologies have been applied by the food industry to control food spoilage and disease causing microorganisms. Due to changing consumer expectations for fresh and safe food, there is a need for alternative processing technologies that are clean and green with no harmful residual effects. Light acting on some natural plant compounds produces reactive oxygen species which are lethal to some pathogens and this phenomenon of photosensitization would be an attractive alternative treatment to chemical preservatives for the decontamination of food and extending shelf life of fresh produce. Curcumin is a natural polyphenolic compound from the spice turmeric (Curcuma longa L.) and has been identified as an efficient photosensitizer for inactivation of a broad range of food related microorganisms. Curcumin-based photosensitization has shown promise in extending the shelf life of fresh date fruit, oysters, salads and other minimally processed foods. It has been very effective against pathogenic bacteria such as Escherichia coli O157:H7, Listeria monocytogenes and Vibiro parahaemolyticus. It has significantly reduced the Aspergillus flavus conidia, hyphae and carcinogenic fungal toxin aflatoxin B1 in maize kernels. The promising results obtained from the application of photoactivated curcumin in food systems suggest that this treatment could be an efficacious and cost-effective method to inactivate a broad range of food borne pathogens.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115442416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Application of surgical curettage combined with photodynamic in refractory plantar wart 手术刮除结合光动力治疗难治性足底疣的应用

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2525314

Kai Huang, Shuang Zhao, Jinmao Li, Mingliang Chen, Fangfang Li, Juan Su, Wei Shi, Xiang Chen

引用次数: 0

Hematoporphyrin mono-methylether photodynamic therapy (HMME-PDT) in treating port-wine stains (PWS) with Chinese patients 血卟啉单甲基醚光动力疗法(HMME-PDT)治疗葡萄酒斑(PWS)

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2525586

Dan C. Li, Xiang Nong, Tian-wen Fang, T. Zhao

{"title":"Hematoporphyrin mono-methylether photodynamic therapy (HMME-PDT) in treating port-wine stains (PWS) with Chinese patients","authors":"Dan C. Li, Xiang Nong, Tian-wen Fang, T. Zhao","doi":"10.1117/12.2525586","DOIUrl":"https://doi.org/10.1117/12.2525586","url":null,"abstract":"Objective: To observe the efficiency and safety of hematoporphyrin mono-methylether photodynamic therapy (HMME-PDT) in treating port-wine stains (PWS) with Chinese patients, and to evaluate the advantage of photograph、VISIA-CRTM system, and dermoscopy in efficacy evaluation. Method:52 patients were treated in our hospital over the past two years with HMME-PDT ,among which, 15 cases were pink type, 29 cases were purple type and remain 8 cases were Nodular thickening type. Initially, All patients received an intravenous injection of 5 mg/kg HMME ,and then the lesion areas of the patients were exposed to 532 nm LED green light after 10 minutes. The irradiation power density was range between 80–95 mW/cm2. By utilization of photograph、VISIA-CRTM system, and dermoscopy to evaluate the clearance after treatments, and then informing the patients to rank the pain level during the treatment via VAS scale, and recording the side effects.Result:After 1-7 times treatments.13 of the 52 cases were cured (25.00%) , 14 cases showed a good efficacy (26.92%), 16 cases indicates alleviation (30.77%), while 9 cases displayed no efficacy (17.31%).By observation, it showed that the pain level each patient could endure were distinct, and it’s remarkable that when receiving consecutive 11-12min of treatment, most of patients have showed with severe pain (according to VAS scale). The side effects after treatment mainly displayed with edema，crust，hyperpigmentation.Conclusion: it shows that after treating with HMME-PDT ,the efficacy is remarkable and with advantage of safety and less side effects, which worthwhile for further research and promotion.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122276161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0