World Congress of the International Photodynamic Association最新文献_第4页

Assessment of the analgesic effect of compound lidocaine cream in patients with port-wine stain treated by hemoporfin photodynamic therapy 复方利多卡因乳膏对oporfin光动力治疗波特酒色斑患者的镇痛效果评价

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2523472

Lian Liu, Zihui Zhang, Xian Jiang

{"title":"Assessment of the analgesic effect of compound lidocaine cream in patients with port-wine stain treated by hemoporfin photodynamic therapy","authors":"Lian Liu, Zihui Zhang, Xian Jiang","doi":"10.1117/12.2523472","DOIUrl":"https://doi.org/10.1117/12.2523472","url":null,"abstract":"Background Port-wine stain (PWS) is a congenital vascular malformation, frequently appearing at the face and neck. Hemoporfin photodynamic therapy (HMME-PDT) is a new technology for treating PWS. Pain is one of the major and intractable side-effects in this treatment. Compound lidocaine cream is widely utilized for local anaesthesia for good curative effect. Objectives To investigate the degree of pain in the patients treated by HMME-PDT and evaluate the analgesia effects of compound lidocaine cream. Methods Patients were divided into two groups randomly. For treatment group, the compound lidocaine cream with the dose of 2g/cm2 was occluded for 2 hours before treatment, and placebo for control group. We used ChangHai rating scale to record pain scores every minute during the treatment. Results 78 patients were included and divided into test group and control group. Pain occured at the beginning of treatment and increased in the first 16 minutes by time, then stabilized. There was a significant difference between the two groups about the association of pain and treatment time (P＜<0.05), but no significant difference of pain scores at each minute(P <0.05). Different factors such as location, size and types of lesion were compared and showed no significant difference of pain scores. Conclusions From this trial, we found that the pain during treatment started at the beginning and got worse in the first 16 minutes by time, then stabilized. Accordingly, compound lidocaine cream could not release the pain during treatment.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"11 7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114223989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Combination light-based therapies to treat pancreatic cancer: a proof of concept 结合光疗法治疗胰腺癌:概念验证

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2525305

A. Ney, I. Mahamed, Andres Garcia-Sampedro, P. Selbo, P. Sancho, A. MacRobert, S. Pereira, P. Acedo

{"title":"Combination light-based therapies to treat pancreatic cancer: a proof of concept","authors":"A. Ney, I. Mahamed, Andres Garcia-Sampedro, P. Selbo, P. Sancho, A. MacRobert, S. Pereira, P. Acedo","doi":"10.1117/12.2525305","DOIUrl":"https://doi.org/10.1117/12.2525305","url":null,"abstract":"Pancreatic ductal adenocarcinoma remains one of the worst types of cancers mainly due to its late diagnosis, lack of effective therapies for advance disease and high chemoresistance. Novel therapeutic options that could improve patient quality of life and overall survival are therefore imperative. In this study, we describe the use of an original strategy based on photochemical internalisation (PCI) technology for pancreatic cancer treatment. Subcellular localisation of the photosensitiser meso-tetraphenylporphine-disulfonate (TPPS2a) was performed in PANC-1 cells, showing its preferential accumulation in lysosomes. Treatments with increasing concentrations of the ribosome-inactivating protein saporin or TPPS2a alone were compared with PCI-saporin. Metabolic activity and cell viability of PANC-1 cells were determined 96h post-illumination by MTT and trypan blue assays, respectively. Our results show that PCI using the photosensitiser TPPS2a, synergistically enhances the cytotoxic effects of saporin in PANC- 1 cells and could offer more effective treatment options for pancreatic cancer.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132038287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photodynamic therapy with a novel zinc phthalocyanine: an innovative approach to hepatocellular carcinoma treatment 一种新型酞菁锌光动力疗法:一种治疗肝细胞癌的创新方法

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2522169

Racheal O. Ogbodu, B. Nitzsche, M. Höpfner

引用次数: 0

Photodyamic therapy in combination with radio frequency electromagnetic field-mediated hyperthermia on cancer cells 光动力疗法联合射频电磁场介导的热疗对癌细胞的作用

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2525979

E. Gyenge, Manuel Murbach, N. Brader, N. Kuster, C. Maake, H. Walt

{"title":"Photodyamic therapy in combination with radio frequency electromagnetic field-mediated hyperthermia on cancer cells","authors":"E. Gyenge, Manuel Murbach, N. Brader, N. Kuster, C. Maake, H. Walt","doi":"10.1117/12.2525979","DOIUrl":"https://doi.org/10.1117/12.2525979","url":null,"abstract":"Background: Greater sensitivity of cancer cells upon a combination of hyperthermia (HT) with conventional cancer treatment is known but clinically not widely used yet. However, HT application procedures are becoming more refined and therefore more interesting for clinical applications in the future. In our study, we treated three different cancer cell lines with photodynamic therapy (PDT) in combination with radio frequency electromagnetic field (RF-EMF) - mediated HT. Methods: PDT treatment was performed by using an herbal (hypericin)- and a synthetic photosensitizer (liposomal mTHPC, Foslipos) on prostate carcinoma PC-3, osteosarcoma 143-B Lac-Z and head and neck cancer UMB-SCC 745 cell lines. HT was generated either by a commercially available incubator or by RF-EMF exposure. After these experiments, cell viability was monitored with MTT assays. Results: We observed improved PDT effects in all cell lines by using RF-EMF mediated HT. In addition, we could show that despite the same temperature ramping, pulse modified RF-EMF mediated HT exhibited significantly more impact compared with sinus modified RF-EMF mediated HT or common HT. Conclusion: RF-EMF mediated HT seems to create significant effects for PDT on various cancer cells in vitro, which are worth to be explored in greater detail in the future.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127291618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Novel mechanism of dendritic cell maturation by dying/death tumor cells via photodynamic modulation of type 1 interferon pathway 1型干扰素途径光动力调节肿瘤细胞死亡/死亡树突状细胞成熟的新机制

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2525429

M. Lamberti, F. Mentucci, V. Rivarola, M. Maccioni, N. B. Rumie Vittar

{"title":"Novel mechanism of dendritic cell maturation by dying/death tumor cells via photodynamic modulation of type 1 interferon pathway","authors":"M. Lamberti, F. Mentucci, V. Rivarola, M. Maccioni, N. B. Rumie Vittar","doi":"10.1117/12.2525429","DOIUrl":"https://doi.org/10.1117/12.2525429","url":null,"abstract":"During the past decades, a growing body of evidence clearly indicates that type I IFNs (IFN-1) play a pivotal role in naturally occurring and therapy induced immune responses to cancer. In this context, we describe here a novel effect of photodynamic therapy (PDT): besides its potential to induce apoptosis, PDT elicited an autocrine/paracrine activation of IFN-1 pathway. In the current work, B16-OVA cells were sensitized with Me-ALA-induced protoporphyrin IX (PpIX) which preferentially localized in the endoplasmic reticulum prior to irradiation. Subsequent photoactivation of PpIX with red-light irradiation ignificantly stimulated tumor cells to induce autocrine IFN-1 transcription, concurrently with IRF-3 phosphorylation, at levels that were capable of activating STAT1 and enhancing ligand receptor (cGAS) and ISGs (CXCL10, MX1, ISG15) expression. Among the cellular and molecular pathways identified so far, type I IFNs are critical components for the host immune response against tumor, more specifically for the dendritic cell (DC) compartment. In this sense, PDT-treated melanoma cells induced paracrine IFN-1-dependent phenotypic maturation of monocyte-derived dendritic cells (DCs) by enhancing co-stimulatory signals (CD80, MHC-II) and tumor-directed chemotaxis (transwell migration assay). Collectively, our findings strongly demonstrate the effects of a novel danger signal released by cancer cells undergoing PDT on the maturation and activation of DCs, highlighting the potential added value of PDT in adoptive immunotherapy protocols.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129611954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence colon tumor imaging by i.v. administered indocyanine green in a rat model of colon carcinogenesis 大鼠结肠癌模型静脉注射吲哚菁绿的结肠肿瘤荧光成像

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2528096

N. Onda, Reiko Mizutani-Morita, Susumu Yamashita, Miho Kojima, Toshinori Yoshida, M. Shibutani

{"title":"Fluorescence colon tumor imaging by i.v. administered indocyanine green in a rat model of colon carcinogenesis","authors":"N. Onda, Reiko Mizutani-Morita, Susumu Yamashita, Miho Kojima, Toshinori Yoshida, M. Shibutani","doi":"10.1117/12.2528096","DOIUrl":"https://doi.org/10.1117/12.2528096","url":null,"abstract":"Tumor imaging using indocyanine green (ICG) has been reported in a variety of tumor types. Currently, there are reported several possible mechanisms of the tumor imaging by IV ICG injection. We previously reported that the tumor tissue preference of the ICG in a mouse xenograft tumor model using human colon cancer cell line is responsible for the tumor cellular uptake of ICG. We also reported that the tissue preference of the ICG in a mouse model of colitisassociated colon cancer can be attributable to the tumor interstitial uptake of ICG. The aim of the present study is to investigate the tumor imaging capability and the imaging mechanism of IV administered ICG in a rat colon carcinogenesis model. Fluorescence imaging experiments were carried out one day after the IV injection of ICG. The ICG fluorescence was observed in the colon tumor tissues, with sufficient tumor to normal tissue ratio. Fluorescence endoscopy detected these ICG+ colon tumors in vivo. High magnification imaging of excised colon tissues revealed that ICG fluorescence in the tumor tissues was localized in the stromal cells at the vascular interstitial tissue at the luminal surface. Collectively, these results suggest that fluorescence imaging following the IV administration of ICG can detect the colon tumor tissues in the current model. The tumor tissue preference of ICG is likely to be responsible for the perivascular stromal cellular uptake of ICG. Our previous and present data will be helpful in exploring the appropriate clinical applications of the tumor imaging by IV ICG administration.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128606706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Topical photodynamic therapy with 5-aminolevulinic acid in the treatment of cervical low grade squamous intraepithelial lesion 5-氨基乙酰丙酸局部光动力疗法治疗宫颈低级别鳞状上皮内病变

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2525485

L. Gu, Zubei Hong, Anyue Wu, W. Cang, Mengxing Cheng, W. Di, L. Qiu

引用次数: 0

Antimicrobial photodynamic therapy action over pathogens linked with healthcare-associated infections in patients with chronical periodontal disease 抗菌光动力疗法对慢性牙周病患者与卫生保健相关感染相关病原体的作用

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2525943

K. I. Sakiyama, Adenir F. Pinto, M. Saleh, R. Prates, R. S. Navarro, A. Baptista, R. H. Marques, S. Núñez

{"title":"Antimicrobial photodynamic therapy action over pathogens linked with healthcare-associated infections in patients with chronical periodontal disease","authors":"K. I. Sakiyama, Adenir F. Pinto, M. Saleh, R. Prates, R. S. Navarro, A. Baptista, R. H. Marques, S. Núñez","doi":"10.1117/12.2525943","DOIUrl":"https://doi.org/10.1117/12.2525943","url":null,"abstract":"Antimicrobial Photodynamic Therapy (aPDT) for periodontal disease (PD) treatment has been studied for more than three decades, but there is no consensus among researchers about its role on PD control. PD is an oral infection linked with a variate of systemic diseases affecting mainly immunocompromised and elderly patients. The aim of this study is to evaluate aPDT for PD control and to analyze the periodontal microbiological profile regarding healthcare-associated infections (HAI). Six patients were enrolled in this study and the microbiological profile of 36 sites were evaluated in different moments: initial, after scaling and root planing, after aPDT and one-week post-treatment. aPDT was performed with 100μM water solution of methylene blue (MB), and a diode laser (100mW, 660nm, 90s per point, 9J). After periodontal therapy the pockets were irrigated with hydrogen peroxide (3%), washed with water, and MB was applied when no bleeding was detected. To collect the periodontal pocket content, a sterilized paper point was placed inside the pocket for 30s. The material was transferred to a vial with transport culture medium and sent to a microbiological facility to be analyzed blindly. The results showed the presence of pathogens linked with HAI as Acinetobacter baumannii, Candida albicans e Serratia marcescens immediately after therapy, but one week after treatment, none of them were detected and the periodontal index of all patients improved. This preliminary result point out that aPDT may not only improve periodontal clinical conditions, but also can diminish the systemic-health threat represented by this disease.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"78 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126117287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

5-aminolevulinic acid as a potential contrast agent for image-guided surgery in pancreatic cancer 5-氨基乙酰丙酸作为胰腺癌影像引导手术的潜在造影剂

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2525084

P. Labib, E. Yaghini, B. Davidson, A. MacRobert, S. Pereira

{"title":"5-aminolevulinic acid as a potential contrast agent for image-guided surgery in pancreatic cancer","authors":"P. Labib, E. Yaghini, B. Davidson, A. MacRobert, S. Pereira","doi":"10.1117/12.2525084","DOIUrl":"https://doi.org/10.1117/12.2525084","url":null,"abstract":"Introduction: Pancreatic cancer often recurs following surgery suggesting new operative approaches are required. Fluorescence-guided surgery aims to assist surgeons in identifying tumour intraoperatively to facilitate complete resection. However, the ideal contrast agent for this purpose is not yet determined. The Rose criterion states that accurate imageguided surgery requires a Tumour-to-Background Ratio of contrast agent greater than 5. We investigated the potential of 5-aminolevulinic acid (ALA) for this purpose. Methods: Pancreatic cancer cell lines CFPAC-1 and PANC-1 were compared with the control pancreatic ductal cell line H6c7. Cells were seeded on day 1 and fluorescence measured on day 4 following 4, 8, 24 or 48 hours incubation with 0.25, 0.50, 0.75 or 1.00mM ALA. Fluorescence was measured using a plate reader and microscopy. Results: The maximum ALA-induced fluorescence for CFPAC-1 and PANC-1 was achieved after 48 hours incubation with 0.50mM ALA. Compared to cells incubated without ALA, a relative fluorescence increase of 39.4-fold in CFPAC-1 and 2.7-fold in PANC-1 was seen. ALA concentrations above 0.50mM did not result in higher fluorescence. In contrast, the control cell line H6c7 showed progressively increasing fluorescence with increasing ALA concentrations. The highest cancer/control cell fluorescence ratios for ALA were after 48 hours incubation with 0.25mM ALA; 122.9 in CFPAC-1 and 9.7 in PANC-1. Conclusion: ALA-induced fluorescence in CFPAC-1 is significantly higher than the control cell line H6c7. PANC-1 achieved only mildly increased fluorescence compared to H6c7. ALA has the potential to provide an adequate level of fluorescence for image-guided pancreatic surgery in ALA-susceptible cancers.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131912162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Topical hydrogen peroxide as a neoadjuvant treatment in the surgical excision of non-melanoma skin cancers 局部过氧化氢作为非黑色素瘤皮肤癌手术切除的新辅助治疗

World Congress of the International Photodynamic Association Pub Date : 2019-08-07 DOI: 10.1117/12.2528241

K. Jordan, N. Mundi, C. Moore

{"title":"Topical hydrogen peroxide as a neoadjuvant treatment in the surgical excision of non-melanoma skin cancers","authors":"K. Jordan, N. Mundi, C. Moore","doi":"10.1117/12.2528241","DOIUrl":"https://doi.org/10.1117/12.2528241","url":null,"abstract":"Introduction: Hydrogen peroxide is used as a topical antiseptic and hemostatic agent. At higher concentrations, it can induce cell death and has recently been reported to be effective in treating seborrheic keratosis. This study examines the effectiveness of topical hydrogen peroxide to shrink non-melanoma skin cancers of the head and neck prior to excision with the goals of minimizing cost and morbidity. Methods: The protocol involves rubbing a solution of 33% hydrogen peroxide into the lesion and a 1 cm border with a cotton tip applicator until blanching is observed. The process can be repeated after one hour and weekly reapplications to a maximum of three times are included in this study group. At one month from the initial application, the remaining lesion is resected with primary closure. The specimen is sent to pathology for histological analysis and final diagnosis. The study will accrue 50 patients with one or more lesions per patient. Measurements of lesion size are recorded by tracing the border on to clear acetate film at each visit. Results: Initial results from first six patients found a range of responses from no size change to no visible lesion remaining for excision. All excised specimens have had negative margins histologically. Summary: Topical hydrogen peroxide is a simple and effective treatment for reducing the size of non-melanoma skin cancers prior to excision.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123635924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1