World Congress of the International Photodynamic Association最新文献

{"title":"The role of proteoglycan genes in PDT-resistant HEp-2 cells","authors":"C. Pacheco-Soares, Roberta Kelly de Faria Souza, Isabel Chaves Silva Carvalho, Renata A. Canevari, B. H. Godoi, Carlos Dailton G. O. Moraes, R. Ricci, Juliana Ferreira Strixino","doi":"10.1117/12.2528183","DOIUrl":"https://doi.org/10.1117/12.2528183","url":null,"abstract":"Optimizing the parameters of PDT for specific cancer cells by identifying the mechanisms involved in the process represents a considerable tool for the development of photosensitizer delivery systems and the improvement of PDT targets for cancer. Proteoglycans, important constituents of extracellular matrix (ECM) play an important role in both progression and inhibition of tumor development. This study determines by gene expression whether proteoglycan are associated with PDT resistance observed in human epidermoid larynx carcinoma cells (HEp-2). We conclude that PDT increases the expression of DECORIN and BIGLYCAN genes, involved in metastatic dissemination, leading to inhibitory effect growth in Hep-2 cells","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"108 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115259534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorodynamic diagnosis of thoracic malignancies","authors":"Y. Ohsaki, Ryohei Yoshida, Takaaki Sasaki, S. Okumura, Yoshinori Minami, Yoshihiro Kazebayashi, N. Hirai, K. Ishibashi, Nana Yoshida, Yasushi Yamamoto, M. Kitada","doi":"10.1117/12.2527526","DOIUrl":"https://doi.org/10.1117/12.2527526","url":null,"abstract":"In the area of neurosurgical tumor diagnosis, clinical research on photodynamic diagnosis using 5-aminolevulinic acid (5-ALA) has already been undertaken, and the safety of 5-ALA has also been demonstrated. 5-ALA is approved in the diagnosis of brain tumor and bladder tumor by the Ministry of Health, Labour and Welfare. 5-ALA is a natural amino acid contained in a living body and is a precursor of hemoglobin. When absorbed into the body by oral ingestion, porphyrin synthesis by heme synthesis is specifically performed in a tumor-specific manner, and accumulation of fluorescent protoporphyrin IX (pp IX) is observed. However, pp IX is metabolized and disappeared in normal tissues. When tumor tissue is irradiated with excitation light, at the site where pp IX accumulates, it exhibits red fluorescence. We applied 5-ALA for photodynamic diagnostic in thoracic malignancies.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123279388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photochromic antifolate for light-activated chemotherapy","authors":"C. Matera, Alexandre M. J. Gomila, Núria Camarero, M. Libergoli, Concepció Soler, P. Gorostiza","doi":"10.1117/12.2522128","DOIUrl":"https://doi.org/10.1117/12.2522128","url":null,"abstract":"Although cytotoxic chemotherapy is one of the primary pharmacological treatments for chronic hyperproliferative diseases such as cancer and psoriasis, its efficacy and tolerability are in many cases dramatically limited by off-target toxicity. A promising approach to improve these therapies is to activate the drugs exclusively at their desired place of action. In fact, in those diseases that would benefit from a highly localized treatment, a precise spatiotemporal control over the activity of a chemotherapeutic agent would allow reducing the concentration of active compound outside the targeted region, improving the tolerability of the treatment. Light is a powerful tool in this respect: it offers unparalleled opportunities as a non-invasive regulatory signal for pharmacological applications because it can be delivered with high precision regarding space, time, intensity and wavelength. Photopharmacology represents a new and emerging approach in this regard since the energy of light is used to change the structure of the drug and hence to switch its pharmacological activity on and off on demand. We describe here phototrexate, the first light-regulated inhibitor of the human DHFR. Enzyme and cell viability assays demonstrated that phototrexate behaves as a potent antifolate in its cis configuration, obtained under UVA illumination, and that it is nearly inactive in its dark-relaxed trans form. Experiments in zebrafish confirmed that phototrexate can disrupt folate metabolism in a light-dependent fashion also in vivo. Overall, phototrexate represents a potential candidate towards the development of an innovative photoactivated antifolate chemotherapy.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"118 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125011573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of medical imaging in the context of photodynamic therapy","authors":"C. Dupont, M. Vermandel, S. Mordon","doi":"10.1117/12.2528007","DOIUrl":"https://doi.org/10.1117/12.2528007","url":null,"abstract":"Photodynamic therapy (PDT) is a modality with promising results for the treatment of various cancers. PDT is increasingly included in the standard of care for different pathologies. The role of medical imaging in this context is crucial to better understand how and where to deliver the therapy but also to observe the different mechanisms involved in the effects on tumors. At different stages of delivery, PDT requires imaging to plan, evaluate and monitor treatment. In this paper, we review the contribution of Magnetic Resonance Imaging or Positron Emission Tomography for planning and therapeutic monitoring purposes. Several solutions have been proposed to plan PDT from imaging. MRI and dedicated segmentation algorithm have been recently proposed to plan interstitial PDT with stereotactic localization and light diffusion simulation capabilities. Additionally, photosensitizer biodistribution has been evaluated with radiolabeled photosensitizers. The effects of PDT delivery have also been explored with specific Magnetic Resonance Imaging or Positron Emission Tomography radiopharmaceuticals to evaluate the effects on cells (apoptosis, necrosis, proliferation, metabolism) or vascular damage. The contribution of medical imaging in the context of photodynamic therapies is important and continues to increase. Using morphological or molecular imaging has to be considered for future developments of PDT.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129951213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of destruction effect of blood vessels after photodynamic therapy in a model of chorioallantoic membrane","authors":"G. Arthuzo, V. Bagnato, H. Buzzá","doi":"10.1117/12.2526155","DOIUrl":"https://doi.org/10.1117/12.2526155","url":null,"abstract":"Photodynamic therapy, a technique used for several diseases, when carried out in blood vessels, leads to their destruction. However, vessel recovery is observed some time later, which can be an angiogenic process (formation of new blood vessels) induced by the therapy itself or blood reperfusion. For the investigation of this vascular process after photodynamic therapy, the chorioallantoic membrane (CAM) model of chicken eggs was used. Photodynamic therapy was performed on membrane vessels with the Photogem photosensitizer, at a concentration of 10 μg/mL, and light subdoses to avoid leading the embryo to death. Light doses of 6 and 15 J/cm2 were established for the experiments and a decrease in vessel density 3 hours after photodynamic therapy was observed, with an increase 24 hours later. For quantification of these effects, an equation was determined and a routine of MATLAB was designed to determine the percentage of area occupied by blood vessels in the images, which were performed before, every 30 minutes for the first 3 hours after treatment and 24 hours later. Furthermore, for an analysis of the distribution of large and small vessels, the length and diameter of each vessel in the images were measured with the ImageJ software, which enabled to verify that the smaller vessels are most affected 3 hours after the therapy, with an increase in the number of these vessels after 24 hours.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"117 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122325648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic response of experimental choroidal neovascularization in mice with photodynamic/photothermal therapy using ICG loaded gold nanorods as a photosensitizer","authors":"Rupesh Singh, Julia C. Batoki, B. Anand-Apte","doi":"10.1117/12.2525194","DOIUrl":"https://doi.org/10.1117/12.2525194","url":null,"abstract":"We examined the efficacy of Indocyanine Green (ICG) loaded plasmonic gold nanorods (AuNP) assisted combined photothermal and photodynamic therapy for laser induced choroidal neovascularization (CNV) in pigmented mice. Eight C57BL/6 wild type pigmented mice were divided into two treatment groups. Each mouse received multiple 532 nm laser induced CNV in both eyes. Control mice were untreated while treatment group received intravenous injection of ICG loaded gold nanorods on day 7 prior to single near-infrared (NIR 808 nm) laser treatment session. For assessing progression of CNV lesion volume, in-vivo ocular imaging was performed on each animal at day 1, 7, and 14 using optical coherence tomography volumetric imaging. Early and late phase fluorescence angiography was performed for CNV leakage analysis. Infrared reflectance imaging and were used to analyze in-vivo laser injury and healing assessment. ICG-AuNP assisted combined photothermal/photodynamic therapy resulted in significantly increased healing and decreased fluorescein leakage at the CNV site compared to control mice who did not receive NIR laser treatment. Enhanced permeability and retention of AuNP assists in localization of therapeutic agents to the CNV lesion, which allows for targeted therapy while minimizing laser induced damage to healthy tissues.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129300146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"5-ALA induces the distribution of endogenous PpIX fluorescence in cervical lesions","authors":"Xiaoli Lu, Guangzhi Liu, Liyun Yang, Guiqin Chen, L. Cheng, B. Mao, Ziyi Zhao, Qiuyun Yang","doi":"10.1117/12.2525359","DOIUrl":"https://doi.org/10.1117/12.2525359","url":null,"abstract":"Objective: To investigate the distribution of ALA in Cervical lesion tissue by tissue frozen section and fluorescence microscopy. Methods: 20% of 5-ALA were topically applied to the cervix.Biopsies were taken from the lesions for histology examination, Fluorescence microscopy was performed methods in these frozen section of cervical tissue to observe the distribution of ALA and the result was analyzed semi-quantitatively. Results: The maximal Protoporphyrin IX (PpIX) fluorescence was observed in the most serious tissue with cervical lesions .fluorescence photometry corroborated these results. Conclusion: ALA is selectively accumulated in cervical lesions, and the more severe the lesion, the easier it is to accumulate.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129445891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Devices based on light emitting fabrics dedicated to PDT preclinical studies","authors":"E. Thecua, L. Ziane, G. Baert, P. Deleporte, B. Leroux, A. Kumar, M. Baydoun, O. Morales, N. Delhem, S. Mordon","doi":"10.1117/12.2525701","DOIUrl":"https://doi.org/10.1117/12.2525701","url":null,"abstract":"Whether preclinical studies either involve a cell or animal model, the distribution of light plays a determinant role in the reproducibility of results of photodynamic therapy (PDT) studies. Unfortunately, only few illumination devices dedicated to preclinical studies are available and are for the most, very expensive. Most research teams use home-made solutions that may not always be reproducible because of undefined light distribution, additive thermal emission, or unsuitable for shapes and volumes to illuminate. To address these issues, we developed illumination devices dedicated to our preclinical studies, which embed knitted light emitting fabrics (LEF) technology. LEF technology offers a homogeneous light distribution, without thermal emission and can be coupled with various light sources allowing investigation of several PDT modalities (irradiance, wavelength, illumination duration/mode). For in-vitro studies, we designed light plates, each allowing illumination of up to four 96-cells plates. For in-vivo studies, we designed mice boxes allowing three animals placement in prone position, equally surrounded by LEF and ensuring homogeneous extracorporeal illumination. Optical validation was performed and reproducibility of both preclinical systems were assessed. Both systems can deliver homogeneous light with an irradiance that can reach several mW/cm2, with varying durations and wavelengths. First results of preclinical studies demonstrate a high reproducibility, with an easy setup, and a great adaptability of illumination modalities with these devices based on light emitting fabrics.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130018492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light emitting fabrics for photodynamic treatment of vulvar primary extramammary Paget's disease","authors":"E. Thecua, F. Lecomte, L. Ziane, A. Vignion-Dewalle, C. Maire, C. Vicentini, Henry Abirached, Delphine Staumont, Laurent Mortier, Serge R. Mordon","doi":"10.1117/12.2525609","DOIUrl":"https://doi.org/10.1117/12.2525609","url":null,"abstract":"Primary Extramammary Paget’s disease (EMPD) is a rare cancer that mainly affects the genital region including vulvar and perianal areas. Without treatment, vulvar EMPD progresses and presents always more erythematous and pruritic plaques, which may become ulcerated and erosive. To control disease progression and symptoms usually experienced by patients, surgical excision is the mainstay of treatment. Unfortunately, even after large surgical excision with intra-operative margin control, recurrences are common [1]. For recurrent patients which undergo multiple resections, severe functional and sexual alterations are described. Only few data are available on the efficacy of alternative conservative treatments, such as laser ablation, radiotherapy, topical chemotherapy and photodynamic therapy (PDT) [2]. To date, none of them can be considered as a solid alternative to surgical excision yet [3]. Nonetheless, multiple clinical cases suggest that PDT could provide the opportunity to treat subclinical lesions, and give some relief on patient’s symptoms of the disease [4-6]. Unfortunately, the benefits of using photodynamic therapy for vulvar EMPD remains a challenge to demonstrate, because of the inhomogeneous illumination of vulvar and perianal areas, and the extreme pain that patients usually experienced during the illumination procedure that may lead to premature end of treatment [7, 8]. Resulting from the knowledge of previous works on actinic keratosis of the scalp treatment with PDT, light emitting fabrics (LEF) technology could address both of the hereinbefore described issues [9-12]. A new medical device based on LEF named PAGETEX dedicated to illumination of vulvar and perianal areas has been developed. The device delivers a homogeneous red light (635 nm) with a low irradiance during 2h30, for a total fluence of 12 J/cm2. The PAGETEX device is being assessed in a clinical study (NCT03713203) which aims to establish PAGETEX- PDT efficacy and tolerability.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132429123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PDT in dermatology: quantification, relevance and comparison of light sources within a few clicks","authors":"A. Vignion-Dewalle, G. Baert, E. Thecua, F. Lecomte, C. Vicentini, L. Mortier, S. Mordon","doi":"10.1117/12.2528108","DOIUrl":"https://doi.org/10.1117/12.2528108","url":null,"abstract":"Photodynamic therapy (PDT) for dermatological conditions relies on the photoactivation of the photosensitizer protoporphyrin IX (PpIX). Many light sources have been or are being investigated. The stronger the overlap of the spectral irradiance of the light source with the absorption spectrum of PpIX is, the more powerful to photoactivate PpIX is the light source. This overlap can be quantified using the PpIX-weighted irradiance, which results from the weighting of the spectral irradiance of the light source by the normalized PpIX absorption spectrum. We have previously developed a freely available website (http://www.oncothai.fr/light-efficiency-calculator/), which aims to compute the PpIX-weighted irradiance of any uploaded spectral irradiance. Through this website, we have assessed a variety of light sources proposed for use in dermatological PDT. The spectral irradiances of these light sources were collected by either extraction from the literature or request to manufacturers. Due to the variations in position, shape, and amplitude of the spectral irradiances with regard to the PpIX normalized absorption spectrum, a wide range of PpIX-weighted irradiances has been obtained. The maximal PpIX-weighted irradiance was achieved with daylight on a clear sunny day.","PeriodicalId":267589,"journal":{"name":"World Congress of the International Photodynamic Association","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133770428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}