The 2nd International Electronic Conference on Biomedicines最新文献_第4页

In Silico Study to Evaluate the Inhibitory Activity of a Few Phenylethanoid Glycosides on GSK3-β Protein for Faster Diabetic Wound Healing 几种苯乙醇糖苷对糖尿病创面GSK3-β蛋白抑制作用的实验研究

The 2nd International Electronic Conference on Biomedicines Pub Date : 2023-03-08 DOI: 10.3390/ecb2023-14134

Ankit Majie, Rajdeep Saha, B. Sarkar

{"title":"In Silico Study to Evaluate the Inhibitory Activity of a Few Phenylethanoid Glycosides on GSK3-β Protein for Faster Diabetic Wound Healing","authors":"Ankit Majie, Rajdeep Saha, B. Sarkar","doi":"10.3390/ecb2023-14134","DOIUrl":"https://doi.org/10.3390/ecb2023-14134","url":null,"abstract":": Chronic wound resulting from diabetes mellitus is a signiﬁcant cause of amputation world-wide. Secondary infections, lowering of nitric oxide synthase level, reduction of glucose-6-phosphate dehydrogenase levels, improper extracellular matrix remodelling, neuropathy, abnormality of endothelial cell function, and vasculopathy impedes the normal wound healing cycle during diabetes. Multiple studies have concluded that Ser9 phosphorylation causes inhibition of the glycogen synthase kinase-3 β (GSK3-β ) protein, which is essential for faster diabetic wound healing. Hence this protein could be a potential target for molecular interactions with prospective wound-healing molecules. Verbascoside, martynoside, echinacoside, crenatoside, and salidroside are a few phenylethanoid glycosides that have potential wound-healing ability by increasing extracellular matrix synthesis, angiogenesis, keratinocyte migration, and the functioning of macrophages and neutrophils. Thus, the ﬁve glycosides were subjected to molecular docking with GSK3-β protein (PDB ID: 1I09). This study revealed strong binding interactions with GSK3-β (between − 10.2 and − 7.3 kcal/mol) and inhibition constants (between 0.032 and 4.397 µ M) which suggested potent inhibition of the target protein even at lower concentrations of these compounds. Further, the docked complexes were visualized to ﬁnd the interaction of the ligands with the amino acid residues. However, further in vivo and in vitro studies are required to validate the activity of these phenylpropanoid glycosides in diabetic wound healing.","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114221435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Neuroimmunomodulation in Chronic Wound Healing after Treatment with Photodynamic Therapy: The Role of iNOs 光动力治疗后慢性伤口愈合的神经免疫调节:iNOs的作用

The 2nd International Electronic Conference on Biomedicines Pub Date : 2023-03-08 DOI: 10.3390/ecb2023-14135

Lorenzo Notari, P. Nardini, V. Grandi, Alessandro Corsi, N. Pimpinelli, S. Bacci

引用次数: 1

An In Silico Approach to Evaluate the Diabetic Wound Healing Potential of Phenylethanoid Glycoside in Inhibiting the Receptor for Advanced Glycation End Products (RAGE) 用计算机方法评价苯乙醇苷抑制晚期糖基化终产物受体(RAGE)对糖尿病创面愈合的影响

The 2nd International Electronic Conference on Biomedicines Pub Date : 2023-03-08 DOI: 10.3390/ecb2023-14137

Ritika Baidya, B. Sarkar

{"title":"An In Silico Approach to Evaluate the Diabetic Wound Healing Potential of Phenylethanoid Glycoside in Inhibiting the Receptor for Advanced Glycation End Products (RAGE)","authors":"Ritika Baidya, B. Sarkar","doi":"10.3390/ecb2023-14137","DOIUrl":"https://doi.org/10.3390/ecb2023-14137","url":null,"abstract":": Diabetes mellitus (DM) is a chronic metabolic disorder and is associated with impaired wound healing. Non-healing leg and foot ulcers are a frequent signiﬁcant consequence of diabetes and are caused by a combination of inadequate tissue perfusion, suppression of re-epithelialization, and poor collagen production. Receptor for Advanced Glycation End Products (RAGE) is a multi-ligand cell surface molecule that belongs to the immunoglobulin superfamily and is crucial in the pathophysiology of poor wound healing in diabetics. By inhibiting RAGE, a chronic non-healing wound is more likely to undergo angiogenesis, enhance blood supply to hypoxic areas of the wound, and decrease the pro-inﬂammatory reaction and pro-apoptotic signaling. Phenylethanoid glycosides (PhGs) are a class of natural glycosides that possess anti-diabetic, wound-healing, antimicrobial, anti-inﬂammatory, and antioxidant properties. Echinacoside, a phenylethanoid glycoside, has a promising role in wound healing by enhancing angiogenesis, promoting keratinocyte migration and proliferation, and enhancing neutrophil and macrophage activity. Consequently, molecular docking was performed to assess the interaction between Echinacoside and the RAGE receptor (PDB ID: 6VXG). The ligand and receptor had a strong binding interaction, as indicated by the lowest binding energy, which was found to be − 6.1 kcal/mol. To further assess the activity of Echinacoside in diabetic wound healing, in vitro and in vivo studies are needed.","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132102355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomics Approaches for the Discovery of Potential Enzymatic Biomarkers for Early Diagnosis of Breast Cancer 发现乳腺癌早期诊断潜在酶生物标志物的蛋白质组学方法

The 2nd International Electronic Conference on Biomedicines Pub Date : 2023-03-02 DOI: 10.3390/ecb2023-14099

Yingxi Li, N. Hüttmann, Z. Minić, M. Berezovski

{"title":"Proteomics Approaches for the Discovery of Potential Enzymatic Biomarkers for Early Diagnosis of Breast Cancer","authors":"Yingxi Li, N. Hüttmann, Z. Minić, M. Berezovski","doi":"10.3390/ecb2023-14099","DOIUrl":"https://doi.org/10.3390/ecb2023-14099","url":null,"abstract":"Breast cancer (BC) is one of the leading causes of death in Canadian women, with an average survival rate of 5 years after diagnosis. Early detection of BC can greatly improve patient outcomes and survival. However, a non-invasive BC detection method is not currently available in clinics. Recent studies suggest that proteins from small extracellular vesicles (sEVs) could be promising biomarkers for non-invasive BC early-stage diagnosis. sEVs are membrane-enclosed vesicles secreted by cells that drive different stages of carcinogenesis in BC. The purpose of this work was to analyze different published proteomics datasets to identify enzymes that could be potentially used as diagnostic biomarkers. Three cell line studies were compared, and overlapping BC proteins were highlighted with proteins found in sEVs from blood and plasma. In total, 106 proteins were selected based on the cell line studies, of which 40 have been identified in blood/plasma sEVs. These 106 proteins were mostly enriched with cell–cell signaling and DNA repair terms based on GO analysis. Furthermore, these 40 proteins contained 11 enzymes that can be explored as potential BC biomarkers. Future validation of enzymes using cancer cell lines and blood from BC patients remains to be determined.","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115502833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of RF Currents on Cytokines Production in Human Keratinocytes 射频电流对人角质形成细胞细胞因子产生的影响

The 2nd International Electronic Conference on Biomedicines Pub Date : 2023-03-01 DOI: 10.3390/ecb2023-14096

M. L. Hernández-Bule, Elena Toledano-Macías, María Antonia Martínez-Pascual, A. Úbeda, M. Fernández-Guarino

{"title":"Effects of RF Currents on Cytokines Production in Human Keratinocytes","authors":"M. L. Hernández-Bule, Elena Toledano-Macías, María Antonia Martínez-Pascual, A. Úbeda, M. Fernández-Guarino","doi":"10.3390/ecb2023-14096","DOIUrl":"https://doi.org/10.3390/ecb2023-14096","url":null,"abstract":": Wound healing consists of a sequence of coordinated phases: inﬂammation, proliferation, and remodeling. In skin lesions, neutrophils and keratinocytes are the main cell types participating in the inﬂammatory phase, during which the release of mediators intervening in the regulation of the subsequent regenerative phases takes place. These mediators are involved in tissue regeneration through induction of transendothelial migration, enzyme secretion, cell adhesion, and T-Cell activation and cytotoxicity, as well as neutrophil accumulation at the wound site. Among these mediators, the keratinocyte-synthesized chemokines RANTES, MCP-1, MIP-1, and IL-8 stand out. Although therapies applying electromagnetic ﬁelds or electric currents have been shown to have anti-inﬂammatory effects in a variety of experimental models and in patients through reduced production of proinﬂammatory cytokines such as IFN-Υ and increased production of IL-10, the knowledge on the biological basis of these effects is still limited. Previous studies by our group have shown that subthermal treatment with radiofrequency (RF) currents used in capacitive-resistive electric transfer (CRET) therapy promotes the proliferation and migration of various cell types, such as human ADSC (stem cells), ﬁbroblasts or keratinocytes, involved in skin regeneration. This study investigates the effects of in vitro treatment with CRET currents on cytokine production by HaCat human keratinocytes. The results reveal that, compared to sham-exposed controls, RF stimulation induces decreased production of IL-8 and RANTES and increased MCP-1, without signiﬁcantly affecting other chemokines such as MIP-1. Taken together, our results indicate that due to the RF effects on the production of chemokines involved in the modulation of the inﬂammatory phase of wound regeneration, CRET therapy could be effective in the treatment of skin wounds.","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125625720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental Identification of Aberrantly Expressed Long Non-Coding RNAs in Breast Cancer 乳腺癌中异常表达的长链非编码rna的实验鉴定

The 2nd International Electronic Conference on Biomedicines Pub Date : 2023-03-01 DOI: 10.3390/ecb2023-14083

I. Pronina, S. Lukina, V. Loginov, A. Burdennyy, T. Kazubskaya, E. Braga, E. Filippova

引用次数: 0

Nepeta nuda L. Plant Extract Preserves the Morphology of Red Blood Cells Subjected to Oxidative Stress 荆芥提取物对氧化应激下红细胞形态的保护作用

The 2nd International Electronic Conference on Biomedicines Pub Date : 2023-03-01 DOI: 10.3390/ecb2023-14086

S. Petrova, Nikol Mazhdrakova, S. Todinova, V. Strijkova, M. Zhiponova, S. Krumova

引用次数: 0

Generation of iPSC-Derived RGCs for Modeling Dominant Optic Atrophy 生成ipsc衍生的RGCs用于模拟显性视神经萎缩

The 2nd International Electronic Conference on Biomedicines Pub Date : 2023-03-01 DOI: 10.3390/ecb2023-14087

Marta García-López, M. E. Gallardo

引用次数: 0

Research into New Molecules with Anti-Inflammatory Activity 抗炎新分子的研究

The 2nd International Electronic Conference on Biomedicines Pub Date : 2023-03-01 DOI: 10.3390/ecb2023-14095

Abel Vale, M. Lucas, D. Ribeiro, E. Fernandes

引用次数: 0

Signature Garlic Phytochemical as a Potential Anti-Candidal Candidate Targeting Virulence Factors in Candida albicans 大蒜植物化学标志作为潜在的抗白色念珠菌毒力因子候选物

The 2nd International Electronic Conference on Biomedicines Pub Date : 2023-03-01 DOI: 10.3390/ecb2023-14080

Z. Hasan, A. Islam, L. Khan

引用次数: 0