The 2nd International Electronic Conference on Biomedicines最新文献

{"title":"In Silico Study of Mangrove Triterpenoids as SARS-CoV-2 Main Protease Inhibitors","authors":"Ramamoorthy Purushothaman, Ganapathy Vishnuram, T. Ramanathan","doi":"10.3390/ecb2023-14332","DOIUrl":"https://doi.org/10.3390/ecb2023-14332","url":null,"abstract":"","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122207048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Silico Study of the Functional Effects of TNRC6B Polymorphic Loci Associated with the Risk of Developing Uterine Leiomyomas According to Genome-Wide Studies †","authors":"Ola M. Alali","doi":"10.3390/msf2023021032","DOIUrl":"https://doi.org/10.3390/msf2023021032","url":null,"abstract":": Uterine leiomyoma (UL) is the most common benign tumor causing considerable morbidity during the reproductive years in women with contributions from environmental and genetic factors. According to the GWAS studies, there are many genes and polymorphisms that are related with and are somehow responsible for the UL pathogenesis, but the biological mechanisms underlying this association remain unclear. This study aimed to investigate the published GWAS studies of UL to recognize the significant functionality of TNRC6B polymorphism linked with UL. Six SNPs were selected based on the seven GWAS published on their association with UL by PubMed database. For their analyses, including their epigenetic effects, expression and splicing patterns, we used in silico approach and bioinformatics tools (HaploReg, GTEx-portal and Gene Ontology Resource). Based on HaploReg, several epigenetic effects regulating these SNPs were found as: rs12484776 (one motif changed, sixteen enhancers and four DNAs histone markers), rs4821939 (three motifs changed, eleven enhancers, four protein bounds and five DNAs histone markers), rs733381 (two motifs changed, eleven enhancers, one protein bound and two DNAs histone markers), rs12484951 (one motif changed histone markers), rs3830738 (five motifs changed histone markers) and rs17332320 (two motifs changed and two DNAs histone markers). Based on on GTEx, it was inferred that rs12484776, rs4821939, rs733381, rs3830738, rs12484951 and rs17332320 are associated with the expression of genes/in tissues as 4/4, 4/4, 4/4, 3/3, 4/4 and 2/2, respectively. These loci do not regulate the expression level of any genes in the UL pathophysiology important tissues, and are not associated with the alternative splicing traits (sQTL) of any gene in any tissue. Gene Ontology Resource indicated that no statistically significant biological pathways for genes associated with the studied polymorphisms have been identified. The in silico analysis of GWAS TNRC6B gene polymorphisms significant for fibroids have pronounced epigenetic effects and affect the expression of six genes ( RP51042K10.10 , FAM83F , TNRC6B , RP51042K10.13 , SLC25A17 and XPNPEP3 ), which may be the basis of their involvement in the pathophysiology of fibroids.","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128749333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of Potential Protein Biomarkers in a Depressed Chinese Malaysian University Student Using Liquid Chromatography-Tandem Mass Spectrometry","authors":"S. Yap, C. N. Foo, Yang Mooi Lim, Foong Leng Ng, P. Tang, Jagjit Kaur Najar Singh, Sherina Mohd-Sidik, K. Pheh","doi":"10.3390/ecb2023-14089","DOIUrl":"https://doi.org/10.3390/ecb2023-14089","url":null,"abstract":": Depression is a serious psychological disorder with high prevalence rates, especially among university students. Serum proteins related to the immune system and oxygen and lipid transfer could have contributing roles in the development of depression and could act as biomarkers for depression. Currently, there is a lack of accurate biological methods that can be used to diagnose depression. Biomarkers could be an inexpensive and convenient way to predict depression and understand its pathophysiology. This study aimed to screen the serum proteome profile of a depressed student for the identification of potential depression biomarkers. A Malaysian private university student who was recruited from the pre-test study ( n = 10) was further analyzed for serum proteome due to the fact that he was depressed, with scores of 15 out of 27 on the Patient Health Questionnaire (PHQ-9). After depleting the high-abundance proteins from the serum sample, liquid chromatography-tandem mass spectrometry (LC-MS/MS) was performed to identify the expressed proteins. A total of 224 proteins were identified. Globins, globulins, apolipoproteins and glycoproteins were most commonly detected. Here, we show the potential biomarkers that can be used to identify depression vulnerable individuals. These findings may be relevant to the development of new diagnostic and treatment strategies. However, further studies with larger sample sizes and healthy controls are needed to confirm the role of these candidate biomarkers for the prediction and diagnosis of depression.","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116743874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Phytochemical and Pharmacological Review of an Indian Plant: Cissus quadrangularis","authors":"H. Hamid, S. Patil","doi":"10.3390/iocn2023-14514","DOIUrl":"https://doi.org/10.3390/iocn2023-14514","url":null,"abstract":"Cissus quadrangularis (Vitaceae) is a common perennial succulent climber plant belonging to the Vitaceae family. The plant has a strong pharmacological profile with a variety of phytoconstituents and is geographically distributed throughout tropical and subtropical regions of the world. It is prominently found in India, Pakistan, and Bangladesh. The plant is found all over India, but its presence is dominantly observed in states such as Assam, Kerala, Odisha, Madhya Pradesh, Tamil Nadu, and Uttar Pradesh. The plant in India is popularly called ‘Hadjod’ or ‘Asthisamharaka’ and is very well established as a medicine related to the management of bone, muscles, and ligament issues. Traditionally, almost all aerial and underground parts have medicinal value, but the stem is most commonly used. Phytochemicals studies performed on the plant revealed the presence of a variety of constituents, viz., tannins, proteins, carbohydrates, phenol flavonoids, triterpenoids, phytosterols, glycosides, saponins, vitamin C, and alkaloids. In addition, these plants are also a rich source of calcium. The systematic review also established the pharmacological role of the plant as a bone setter and fractured bone healer; its antimicrobial, anti-diabetic, anti-inflammatory, antiobesity, and anti-oxidant effects; bone turnover; cardiovascular and hepatoprotective properties; and many more. The current review article carried out a detailed discussion of its phytochemical and pharmacological potential.","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127783910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Restoration of Arterial Blood Flow Access to Rhomboid Fossa Assists in Left Ventricular Hypertrophy Normalization","authors":"Kirill V Zhukov, Grigorii V. Dudnik, Alexandre A. Vetcher, Bagrat A. Gasparyan, Alexander Y. Shishonin","doi":"10.3390/ecb2023-14551","DOIUrl":"https://doi.org/10.3390/ecb2023-14551","url":null,"abstract":": We have found a logical way to prove a mechanism that allows us to demonstrate the relationship between the restoration of arterial blood flow through the cervical vertebral arteries to the rhomboid fossa and the normalization of left ventricular hypertrophy. The human body is considered a dissipative structure. The process of the restoration of the body should be considered a redirection of energy flows from decay to restoration. It is also necessary to take into account the role of information about the availability of oxygen coming from the rhomboid fossa to the cerebellum. We plan to conduct animal studies and create a mathematical model of the system. This may accelerate the development of this theory.","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124647006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"De Novo Drug Design of Potential Inhibitors of the Receptor-Binding Domain of SARS-CoV-2 Variants","authors":"E. R. Lopez","doi":"10.3390/ecb2023-14371","DOIUrl":"https://doi.org/10.3390/ecb2023-14371","url":null,"abstract":": In this study, novel potential inhibitors of SARS-CoV-2 variants were designed de novo using generative neural networks. The top-performing ligand based on docking performance and ADMET profiles was CID #526. It forms several hydrogen bonds with wild-type SARS-CoV-2, indicating its potential as an inhibitor of the receptor-binding domain. Mutated variants of the RBD also showed good interactions with CID #526, implying the inhibitory properties of our top-performing compound against various variants. Molecular dynamics analysis showed a stable ligand–RBD complex. CID #526 can easily be synthesized using low-cost starting molecules. Overall, the generated ligands merit further investigation to determine their efficacy and safety as a treatment for COVID-19.","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123237261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"De Novo Drug Design of Potential Inhibitors of SARS-CoV-2 Papain-like Protease","authors":"E. R. Lopez","doi":"10.3390/ecb2023-14368","DOIUrl":"https://doi.org/10.3390/ecb2023-14368","url":null,"abstract":": Here, potential inhibitors of the SARS-CoV-2 papain-like protease (PL pro ) are reported. A drug molecule (PL pro -50), designed de novo using generative neural networks, interacts with PL pro via hydrogen bonding, forming a salt bridge, and π – π stacking, making it a promising drug against PL pro . PL pro -50 has an excellent ADMET profile with good absorbability, high clearance, and low toxicity. Molecular dynamics analysis revealed the stability of the receptor–ligand complex of PL pro -50 and PL pro . An organic retrosynthesis study showed the feasibility of PL pro -50 to be synthesized using low-cost starting materials. Further studies should be performed to determine whether the determined drug candidates are efficacious in treating COVID-19 infections.","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114044089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Taraxacum officinale on Glioblastoma Cell Culture and Their Correlation with Hydroxycinnamic Acids Content","authors":"A. Fulga, A. Casian, I. Casian, S. Protopop, V. Gudumac, O. Tagadiuc","doi":"10.3390/ecb2023-14363","DOIUrl":"https://doi.org/10.3390/ecb2023-14363","url":null,"abstract":": Glioblastoma is an aggressive type of CNS tumor. The aim was to evaluate the antitumor activity of Taraxacum officinale (TO) extracts on U-138 MG glial cells and correlate it with the concentration of chicoric (ChA), chlorogenic (CGA), and caftaric (CA) acids (mg/mL) in the extract. TO dry leaves were extracted with DMSO (dimethyl sulfoxide) and ethanol of different concentrations. The concentration of acids was determined by liquid chromatograph (Agilent 1260 with DAD). The viability of U-138 MG cells was assessed by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) test (% of viable cells). The activity of TO was compared with that of doxorubicin. The best antitumor activity was shown by TO extracts prepared with DMSO (110,000 µ g/L —17.3 ± 8%, which contained ChA—8976 × 10 − 6 mg/mL, CGA—316.8 × 10 − 6 mg/mL, CA—1628 × 10 − 6 mg/mL), with 50% ethanol (150,000 µ g/L—13.7 ± 3.2%, containing ChA—52,500 × 10 − 6 mg/mL, CGA—1746 × 10 − 6 mg/mL, CA—8460 × 10 − 6 mg/mL) and with 80% ethanol (40,000 µ g/L—16.1 ± 9%, containing ChA—904 × 10 − 6 mg/mL, CGA—114.4 × 10 − 6 mg/mL, CA—70.4 × 10 − 6 mg/mL). TO extract activity was close to that of doxorubicin. In conclusion, the TO antitumor activity depends on the type of extractant and its concentration, as well as on the content of cinnamic acids.","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122032647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Systematic Review: Ayurvedic Herbal Medicine for Women with Polycystic Ovary Syndrome","authors":"Mansi A. Dhankani, Harshada J. Patil, Amitkumar R. Dhankani","doi":"10.3390/ecb2023-14362","DOIUrl":"https://doi.org/10.3390/ecb2023-14362","url":null,"abstract":"","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128960597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salvia hispanica L. Seeds: A Rare Medicinal Herb and Potential Pharmaceutical Additive","authors":"Amitkumar R. Dhankani, S. Patil","doi":"10.3390/ecb2023-14356","DOIUrl":"https://doi.org/10.3390/ecb2023-14356","url":null,"abstract":"Salvia hispanica is a plant widespread in Central America. Chia is the common name for it. The seeds obtained from the current plant are most usually used. As public health awareness grows around the world, so does the need for functional foods with many health advantages. They are also known as “health food” due to their strong nutritional and therapeutic properties. When the seeds are soaked in a suitable solvent, such as water, they exude a sticky gel-like substance that can be employed as an excipient in both culinary and pharmaceutical compositions. This paper will go over all of the therapeutic benefits of the present plant and its parts, as well as the plant’s use as an ingredient in foods and pharmaceuticals.","PeriodicalId":265361,"journal":{"name":"The 2nd International Electronic Conference on Biomedicines","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115221242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}