{"title":"Extraction of Pectin from Breadnut Fruit (Artocarpus altilis Fosberg.) with Citric Acid","authors":"D. Ardiansyah, Ismiarni Komala, Zilhadia Zilhadia","doi":"10.4108/eai.26-10-2020.2311324","DOIUrl":"https://doi.org/10.4108/eai.26-10-2020.2311324","url":null,"abstract":". Pectin is a complex polysaccharide that is found in the primary cell wall of higher plants. Pharmaceutically, pectin has been used as a gelling agent, thickener, stabilizer emulsifier, and edible coating in the food industry. The purpose of this study was to extract and characterize the isolated pectin from breadnut fruit ( Artocarpus altilis Fosberg). Extraction was conducted by using 1% citric acid (pH adjusted to 2, temperature 90 o C) in the variation of times (2 and 3 hours). Furthermore, the yield and characteristics of pectin were evaluated statistically. The results showed that no significant differences in yield, water content, ash content, equivalent weight, and degree of esterification of pectin when extraction was conducted at different times (2 and 3 hours). Variation time of extraction only showed a significant effect on the methoxyl contents and galacturonic acid contents of pectin.","PeriodicalId":261787,"journal":{"name":"Proceedings of the 1st International Conference on Health Science, ICHS 2020, 26-27 October 2020, Jakarta, Indonesia","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123777678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zilhadia Zilhadia, S. Pambudi, Yusuf Pradana, Arvian Istara
{"title":"The Effect of Boiling Temperature in Beef and Pork Meatball Making Process on DNA Detection using Real Time Polymerase Chain Reaction (RT-PCR) Method","authors":"Zilhadia Zilhadia, S. Pambudi, Yusuf Pradana, Arvian Istara","doi":"10.4108/eai.26-10-2020.2311309","DOIUrl":"https://doi.org/10.4108/eai.26-10-2020.2311309","url":null,"abstract":". Boiling meat in meatball making process can cause DNA degradation. This study aims at determining the effect of boiling temperature on beef and pork DNA detection in meatball samples using Real Time Polymerase Chain Reaction (RT-PCR). DNA of beef, pork, beef meatballs, pork meatballs and beef-pork meatballs boiled at 8° C, 90° C, and 100° C were isolated using the Wizard Genomic DNA Purification Kit and amplified using the RT-PCR. The result has shown DNA of beef meatball, pork meatball, and beef-pork meatball samples heated to 80 o C, 90° C, 100° C can be amplified. Here, the Cycle threshold (Ct) value of the beef meatball, pork meatball, and beef-pork meatball samples heated to 80 °C, 90 °C, 100 °C is significantly different from that of unheated beef and pork (p <0.05). However, there is no significant difference in the Ct value of the beef meatball, pork meatball, and beef-pork meatball samples heated to 80 ° C, 90 ° C, 100 ° C (p≥0.05). It can be concluded that the beef and pork DNA in meatballs boiled at 80 o C to 100 o C is detectable by the RT-PCR. 1.5 volume microcentrifuge tube (Biogenix), micropipette (Biorad), centrifugator (5417R-Eppendrof), vortex (Horiba, Japan), digital waterbath (SB-100 Eyela), UV DNA spectrophotometer (DeNovix®), and analytical scales (Horiba, Japan).","PeriodicalId":261787,"journal":{"name":"Proceedings of the 1st International Conference on Health Science, ICHS 2020, 26-27 October 2020, Jakarta, Indonesia","volume":"62 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122624166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Azrifitria Azrifitria, Harfia Mudahar, N. Fazriyah
{"title":"A retrospective study of the Antibiotics Prophylactic Use in Appendectomy Patient with ATC/DDD and DU 90% Method in Regional General Hospital in Jakarta","authors":"Azrifitria Azrifitria, Harfia Mudahar, N. Fazriyah","doi":"10.4108/eai.26-10-2020.2311323","DOIUrl":"https://doi.org/10.4108/eai.26-10-2020.2311323","url":null,"abstract":"Appendicitis is the most common cause of acute abdomen which requires surgical treatment to prevent worse complications. Inappropriate use of prophylactic antibiotic is one of the triggers surgical site infection. This retrospective study aimed to determine the quantitative analysis of antibiotics prophylactic use according to the anatomical therapeutics chemical classification/defined daily dose (ATCC/DDD) methodology. This research was conducted using cross sectional design with retrospective data collection during January to December 2016 at regional general hospital in Jakarta and data were analyzed using the DDD method and drug utilization (DU) 90%. A total of 119 total patients included as samples in this study. The results showed that the ceftriaxone was prophylactic antibiotic with highest DDD was 46.52 /100 patient-days. Prophylactic antibiotics included in DU 90% segment were ceftriaxone and metronidazole. These study underline the need for antibiotic stewardship to improve the quality of antibiotic use.","PeriodicalId":261787,"journal":{"name":"Proceedings of the 1st International Conference on Health Science, ICHS 2020, 26-27 October 2020, Jakarta, Indonesia","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131715629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sandrawati Sandrawati, N. Nasir, R. Alkaff, L. Hanifah
{"title":"Stigma of People Living with HIV/AIDS and Its Related Factors among Health Students in UIN Jakarta","authors":"Sandrawati Sandrawati, N. Nasir, R. Alkaff, L. Hanifah","doi":"10.4108/eai.26-10-2020.2311307","DOIUrl":"https://doi.org/10.4108/eai.26-10-2020.2311307","url":null,"abstract":". The challenge of HIV/AIDS problems in Indonesia is stigma and discrimination against people living with HIV/AIDS (PLWHA). This study was aimed to determine factors related to stigma to PLWHA among health student in UIN Syarif Hidayatullah Jakarta, Indonesia. This research used a cross sectional study design involving 155 health students that were selected randomly as respondents. Data were collected utilizing questionnaire through google form then analyzed by performing Chi-square test. Results showed that 60.6% of respondents still had stigmatize against PLWHA. Poor knowledge (p value = 0.035), influence of peer attitudes (p value = 0.002), and parent’s attitude (p value = 0.003) had significantly associated with stigma to PLWHA. It is recommended that health students have to improve their knowledge about HIV/AIDS with reliable and valid source of information in order not to be easily influenced by the attitude of parents and peers regarding stigma of PLWHA.","PeriodicalId":261787,"journal":{"name":"Proceedings of the 1st International Conference on Health Science, ICHS 2020, 26-27 October 2020, Jakarta, Indonesia","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123777594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Preformulation Study of 50% Ethanol Extract of Mangosteen Rind (Garcinia mangostana L.)","authors":"Sabrina Dahlizar, Y. Anggraeni, Hanny Narulita","doi":"10.4108/eai.26-10-2020.2311315","DOIUrl":"https://doi.org/10.4108/eai.26-10-2020.2311315","url":null,"abstract":". Garcinia mangostana rind containing alpha-mangostin has the potential to be used as an antioxidant. This study aimed to determine the physicochemical properties and stability of alpha-mangosteen in the 50% ethanol extract of mangosteen rind as a parameter in the pre-formulation study. Physicochemical properties were evaluated for specific and nonspecific extract parameters, determining the optimum wavelength and solubility test. The stability test was carried out at a temperature of 45±5°C with relative humidity 75±5% for 21 days, in acidic and alkaline conditions. The alpha-mangostin content was analyzed using a UV-Vis spectrophotometer. The results showed that the extract was purplish brown, has an aromatic smell, and a bitter taste. The dissolved compound in water and ethanol were 62.54±1.09% and 87.05±0,43%, respectively, the specific density of 5% extract was 1.036, loss on drying 6.66±0,11%, ash content 5.07±0.23%, ash content insoluble in acidic was 0.13±0.02%, alpha-mangosteen content in the extract was 3.85±0.03%. The solubility test showed that alpha-mangosteen in the extract has a solubility value of 1: 16064 in water. The stability test showed that the levels of alpha-mangostin decreased significantly (p≤ 0 .05) at 45±5°C and relative humidity 75±5% for 21 days. Mangosteen rind extract was unstable in the extremely acidic and alkaline conditions. Kruskal-Wallis test showed that the stability test had a significant effect as time went by ( p ≤ 0,05). The results showed that at a temperature of 45±5°C","PeriodicalId":261787,"journal":{"name":"Proceedings of the 1st International Conference on Health Science, ICHS 2020, 26-27 October 2020, Jakarta, Indonesia","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122640357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Atmanto, N. Ambarwati, N. Hidayah, Esti Suntari
{"title":"Students' Environmental Insights on Behavior of Climate Change Mitigation","authors":"D. Atmanto, N. Ambarwati, N. Hidayah, Esti Suntari","doi":"10.4108/eai.26-10-2020.2311322","DOIUrl":"https://doi.org/10.4108/eai.26-10-2020.2311322","url":null,"abstract":". Students' responsibility for the environment is still low and will have an impact on the future of the earth's environment. The purpose of this study was to obtain an overview of the relationship between environmental insight and student behavior in climate change mitigation. The research method used is correlational quantitative survey method. Data obtained through questionnaires and interviews. The sample of students in this study was 60 people from various study programs at the Jakarta State University who were taken randomly. Analysis and interpretation of data shows that there is a positive relationship between students' knowledge of environmental knowledge with climate change mitigation behavior. There is a positive relationship between responsibilities for environmental preservation with student behavior about climate change mitigation. There is a positive relationship between environmental knowledge and environmental conservation responsibility by way of climate change mitigation of students behavior with R is 0.883 and R square is 0.780.","PeriodicalId":261787,"journal":{"name":"Proceedings of the 1st International Conference on Health Science, ICHS 2020, 26-27 October 2020, Jakarta, Indonesia","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127050176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Study of Drug Information Service for Prescription Containing Antibiotic and its Sale without Prescription in Tangerang City Indonesia","authors":"Ambar Listyorini, Yardi Saibi, Nurmeilis Nurmeilis","doi":"10.4108/eai.26-10-2020.2311314","DOIUrl":"https://doi.org/10.4108/eai.26-10-2020.2311314","url":null,"abstract":"Antibiotic resistance can be caused due to its improper use. This study aimed to investigate the profile of information provided by pharmacy staff in Tangerang City to patients with prescriptions containing antibiotics and the actions taken by them on requests for antibiotics without a prescription. This was a crosssectional study. Data were collected from pharmacy through the simulation patient method. Ethical clearance was obtained from the Ethics Committee of the Faculty of Medicine, University of Indonesia. The results showed that of the 55 pharmacies visited by patients, 98.18% of pharmacy staff who dispensed drugs to patients were non-pharmacists. The most information items provided to patients were the frequency of drug use. As many as 89.09% of pharmacy staff gave additional antibiotics asked by patients without prescription. Pharmacy staff services for patient taking antibiotic drugs in Tangerang City need to be reevaluated so that they are in accordance with regulations.","PeriodicalId":261787,"journal":{"name":"Proceedings of the 1st International Conference on Health Science, ICHS 2020, 26-27 October 2020, Jakarta, Indonesia","volume":"115 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129176082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Analysis of Prescribing Phase in Prescription of Pulmonary Tuberculosis Outpatients at Regional General Hospital in Jakarta in 2018","authors":"Farnia Zahra, Azrifitria Azrifitria, Ganda Tampubolon","doi":"10.4108/eai.26-10-2020.2311304","DOIUrl":"https://doi.org/10.4108/eai.26-10-2020.2311304","url":null,"abstract":"Based on PERMENKES No. 72 of 2016 about Pharmaceutical Service in Hospital, this study aimed to determine percentage of patient characteristics, completeness, and accuracies in prescriptions on pharmaceutical and clinical aspects to improve the quality of tuberculosis treatment. A retrospective descriptive study was conducted on 211 pulmonary tuberculosis prescriptions with a purposive sampling method in pharmacy service at a Regional General Hospital in Jakarta from January to December 2018. Most characteristics of patients were male, 26-35 years old, new patients classification, first category tuberculosis treatment, and complete treatment results were 64%, 23%, 92%, 92%, and 90%, respectively. The completeness in pharmaceutical aspects such as dosage form and drug strength were on 51% and 37%, respectively. Accuracies in clinical aspects were 93% for both dose accuracies and frequency of drug administration. Last, there was 2% of drug duplication. Collaboration between physicians and pharmacists plays a significant role in ensuring patient safety and the quality use of medicines.","PeriodicalId":261787,"journal":{"name":"Proceedings of the 1st International Conference on Health Science, ICHS 2020, 26-27 October 2020, Jakarta, Indonesia","volume":"710 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130490806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Description of Earthquake Preparedness Level in Junior High School Community in Majasari District of Pandeglang Regency in 2019","authors":"Anisa Rahmawati, D. Iriani","doi":"10.4108/eai.26-10-2020.2311317","DOIUrl":"https://doi.org/10.4108/eai.26-10-2020.2311317","url":null,"abstract":". Majasari District is located in the high prone zone of earthquake, while the earthquake preparedness simulation never been held there. This endangers the preparedness in facing disasters. This study aim to describe earthquake preparedness level in junior high school community in Majasari district, Pandeglang, Banten Province, Indonesia. Cross sectional study design was conducted with all of principals, teachers and 175 students as samples. The instruments were modified questionaire from LIPI and UNESCO. The result shows the low level of earthquake preparedness of junior high school community and junior high school teachers, while junior high schools students is in moderate category. Earthquake preparedness policy and guideline got the lowest score of all variables, meanwhile attitude toward earthquake preparedness is the lowest. It is suggested Regional Disaster Management Agency (BPBD) Pandeglang to socialize and provide earthquake preparedness training for Junior High Schools in Majasari district.","PeriodicalId":261787,"journal":{"name":"Proceedings of the 1st International Conference on Health Science, ICHS 2020, 26-27 October 2020, Jakarta, Indonesia","volume":"8 8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130489176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Effect of GENTING Booklet in Stunting Prevention on Knowledge, Attitude, and Behavior of Adolescent Mothers","authors":"M. Putri, K. Lestari","doi":"10.4108/eai.26-10-2020.2311348","DOIUrl":"https://doi.org/10.4108/eai.26-10-2020.2311348","url":null,"abstract":". Risk factors that contribute to the stunting prevalence include adolescent marriage, hygiene practices, and insufficient healthy houses. This condition is compounded by the lack of knowledge and behavior of young mothers related to stunting prevention. A lot of efforts have been taken as one of them is to help solving this problem by using the GENTING booklet. The purpose of this study was to increase the knowledge, attitude, and behavior of adolescent mothers to prevent stunting. The pre-experimental research with one group pre-test and post-test were used with purposive sampling in a total of 33 adolescent mothers as respondents and using GENTING booklet and questionnaires as instruments. The Wilcoxon test revealed a significant value on knowledge, attitudes, and behavior, p = 0.000 (p <0.05). Therefore, it can be concluded that the education with GENTING booklet affected the knowledge, attitude, and behavior of the adolescent mothers. The GENTING booklet can be used as a health promotion media as a means to prevent stunting.","PeriodicalId":261787,"journal":{"name":"Proceedings of the 1st International Conference on Health Science, ICHS 2020, 26-27 October 2020, Jakarta, Indonesia","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126721489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}