Biomaterials最新文献_第8页

Multifunctional sericin-based biomineralized nanoplatforms with immunomodulatory and angio/osteo-genic activity for accelerated bone regeneration in periodontitis 具有免疫调节和血管/骨生成活性的多功能丝胶基生物矿化纳米平台可加速牙周炎患者的骨再生

IF 12.8 1区医学

Biomaterials Pub Date : 2024-10-10 DOI: 10.1016/j.biomaterials.2024.122885

Piaoye Ming , Bojiang Li , Qiumei Li , Lingling Yuan , Xueyu Jiang , Yunfei Liu , Rui Cai , Peirong Zhou , Xiaorong Lan , Gang Tao , Jingang Xiao

{"title":"Multifunctional sericin-based biomineralized nanoplatforms with immunomodulatory and angio/osteo-genic activity for accelerated bone regeneration in periodontitis","authors":"Piaoye Ming , Bojiang Li , Qiumei Li , Lingling Yuan , Xueyu Jiang , Yunfei Liu , Rui Cai , Peirong Zhou , Xiaorong Lan , Gang Tao , Jingang Xiao","doi":"10.1016/j.biomaterials.2024.122885","DOIUrl":"10.1016/j.biomaterials.2024.122885","url":null,"abstract":"<div><div>Periodontitis is a chronic inflammation caused by dental plaque. It is characterized by the accumulation of excessive reactive oxygen species (ROS) and inflammatory mediators in the periodontal area. This affects the function of host cells, activates osteoclasts, and destroys periodontal tissue. Treatments such as local debridement or antibiotic therapy for ameliorating the overactive inflammatory microenvironment and repairing periodontal tissues are challenging. This paper reports multifunctional nanoplatforms (Se-CuSrHA@EGCG) based on sericin with ROS-scavenging, immunomodulatory, angiogenic, and osteogenic capabilities. The natural protein sericin, derived from silk cocoons, is used in water/oil emulsification and cross-linking processes to create sericin nanoparticles (Se NPs). Numerous binding sites are present on the surface of Se NPs. Ion-doped hydroxyapatite nanoparticles (Se-CuSrHA NPs) can be constructed using the force between positive and negative charges. After mineralization, an antioxidant coating is formed on the surface using polyethyleneimine (PEI)/epigallocatechin gallate (EGCG). Research conducted both <em>in vitro</em> and <em>in vivo</em> demonstrates that Se-CuSrHA@EGCG NPs can efficiently scavenge ROS, regulate macrophage polarization, increase the secretion of anti-inflammatory cytokines, and balance the immune microenvironment. In addition, Se-CuSrHA@EGCG stimulates angiogenesis, inhibits osteoclasts, and accelerates periodontal tissue repair. Therefore, this is a preferable strategy to accelerate bone regeneration in patients with periodontitis.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"314 ","pages":"Article 122885"},"PeriodicalIF":12.8,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An innovative strategy harnessing self-activating CAR-NK cells to mitigate TGF-β1-driven immune suppression 利用自激活 CAR-NK 细胞减轻 TGF-β1 驱动的免疫抑制的创新战略

IF 12.8 1区医学

Biomaterials Pub Date : 2024-10-10 DOI: 10.1016/j.biomaterials.2024.122888

Seung hun Shin , Young Eun Lee , Han-Na Yoon , Chae Min Yuk , Jun Yop An , Minkoo Seo , Sangwon Yoon , Min-Suk Oh , Sang Chul Shin , Ji Hyung Kim , Yong Jun Kim , Jin-Chul Kim , Song Cheol Kim , Mihue Jang

{"title":"An innovative strategy harnessing self-activating CAR-NK cells to mitigate TGF-β1-driven immune suppression","authors":"Seung hun Shin , Young Eun Lee , Han-Na Yoon , Chae Min Yuk , Jun Yop An , Minkoo Seo , Sangwon Yoon , Min-Suk Oh , Sang Chul Shin , Ji Hyung Kim , Yong Jun Kim , Jin-Chul Kim , Song Cheol Kim , Mihue Jang","doi":"10.1016/j.biomaterials.2024.122888","DOIUrl":"10.1016/j.biomaterials.2024.122888","url":null,"abstract":"<div><div>The dysfunction of natural killer (NK) cells, mediated by transforming growth factor β1 (TGFβ1) within the tumor microenvironment, impedes antitumor therapy and contributes to poor clinical outcomes. Our study introduces self-activating chimeric antigen receptor (CAR)-NK cells that block TGFβ1 signaling by releasing a specifically designed peptide, P6, which targets mesothelin in pancreatic tumors. P6 originates from the interaction sites between TGFβ1 and TGFβ receptor 1 and effectively disrupts TGFβ1's inhibitory signaling in NK cells. Our analysis demonstrates that P6 treatment interrupts the SMAD2/3 pathway in NK cells, mitigating TGFβ1-mediated suppression of NK cell activity, thereby enhancing their metabolic function and cytotoxic response against pancreatic tumors. These CAR-NK cells exhibit potent antitumor capabilities, as evidenced in spheroid cultures with cancer-associated fibroblasts and <em>in vivo</em> mouse models. Our approach marks a substantial advancement in overcoming TGFβ1-mediated immune evasion, offering a promising avenue for revolutionizing cancer immunotherapy.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"314 ","pages":"Article 122888"},"PeriodicalIF":12.8,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mesenchymal stromal cells surface engineering for efficient hematopoietic reconstitution 用于高效造血重建的间充质基质细胞表面工程

IF 12.8 1区医学

Biomaterials Pub Date : 2024-10-10 DOI: 10.1016/j.biomaterials.2024.122882

Huiyang Li , Lifei Ma , Ni Zhu , Xiaoyu Liang , Xinxin Tian , Kaijing Liu , Xue Fu , Xiaoli Wang , Hailing Zhang , Houzao Chen , Qiang Liu , Jing Yang

{"title":"Mesenchymal stromal cells surface engineering for efficient hematopoietic reconstitution","authors":"Huiyang Li , Lifei Ma , Ni Zhu , Xiaoyu Liang , Xinxin Tian , Kaijing Liu , Xue Fu , Xiaoli Wang , Hailing Zhang , Houzao Chen , Qiang Liu , Jing Yang","doi":"10.1016/j.biomaterials.2024.122882","DOIUrl":"10.1016/j.biomaterials.2024.122882","url":null,"abstract":"<div><div>Mesenchymal stromal cells (MSCs) are believed to migrate to injury sites, release chemical attractants, and either recruit local stem cells or modulate the immune system positively. Although MSCs are highly desired for their potential to reduce inflammation and promote tissue regeneration, their limited lifespan restricts their applications. This study presents a simple approach for protecting MSCs with epigallocatechin-3-gallate (EGCG) and magnesium (Mg) based metal-organic framework coatings (E-Mg@MSC). The layer strengthens MSCs resistant to harmful stresses and creates a favorable microenvironment for repair by providing Mg to facilitate MSCs' osteogenic differentiation and using EGCG to neutralize excessive reactive oxygen species (ROS). E-Mg@MSC serves as a treatment for hematopoietic injury induced by ionizing radiation (IR). Coated MSCs exhibit sustained secretion of hematopoietic growth factors and precise homing to radiation-sensitive tissues. In vivo studies show substantial enhancement in hematopoietic system recovery and multi-organ protection. Mechanistic investigations suggest that E-Mg@MSC mitigates IR-induced ROS, cell apoptosis, and ferroptosis, contributing to reduced radiation damage. The system represents a versatile and compelling strategy for cell-surface engineering with functional materials to advance MSCs therapy.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"314 ","pages":"Article 122882"},"PeriodicalIF":12.8,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering cell membrane-camouflaged COF-based nanosatellite for enhanced tumor-targeted photothermal chemoimmunotherapy 基于细胞膜伪装的 COF 纳米卫星工程，用于增强肿瘤靶向光热化疗免疫疗法。

IF 12.8 1区医学

Biomaterials Pub Date : 2024-10-10 DOI: 10.1016/j.biomaterials.2024.122869

Dan Lin , Wenxin Lv , Min Qian , Guangwei Jiang , Xiaojun Lin , Darambazar Gantulga , Yi Wang

{"title":"Engineering cell membrane-camouflaged COF-based nanosatellite for enhanced tumor-targeted photothermal chemoimmunotherapy","authors":"Dan Lin , Wenxin Lv , Min Qian , Guangwei Jiang , Xiaojun Lin , Darambazar Gantulga , Yi Wang","doi":"10.1016/j.biomaterials.2024.122869","DOIUrl":"10.1016/j.biomaterials.2024.122869","url":null,"abstract":"<div><div>Dendritic cells (DCs) activation is crucial for regulating the antitumor immune response. However, the tumor's immunosuppressive environment significantly impedes antigen presentation and DCs maturation, thereby limiting the effectiveness of cancer immunotherapy. To address this challenge, we developed tumor cell membrane-coated covalent organic framework (COF) nanoparticles, loaded with mannose-modified gold nanoparticles and doxorubicin (Dox). This created a cell membrane-camouflaged COF-based nanosatellite designed to enhance tumor-targeted chemoimmunotherapy. The nanosatellite exhibits distinct photothermal properties and releases Dox in a pH-sensitive manner, targeting tumor cells to induce immunogenic cell death (ICD) and expose a wealth of antigens. Crucially, the COF structure is selectively degraded to release mannose-modified gold nanoparticles in the acidic environment. These nanoparticles capture antigens from the ICD and efficiently transport them to lymph nodes rich in DCs, facilitated by mannose receptor mediation. As a result, antigens are effectively presented to DCs, activating the immune response, significantly hindering tumor growth and lung metastasis in mice, and extending survival. This study pioneered innovative nano-preparations aimed at enhancing tumor immunotherapy.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"314 ","pages":"Article 122869"},"PeriodicalIF":12.8,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ROS-responsive injectable hydrogels loaded with exosomes carrying miR-4500 reverse liver fibrosis 负载有携带 miR-4500 的外泌体的 ROS 响应型可注射水凝胶可逆转肝纤维化

IF 12.8 1区医学

Biomaterials Pub Date : 2024-10-10 DOI: 10.1016/j.biomaterials.2024.122887

Huili Yang , Wanshun Wang , Jiacong Xiao , Rong Yang , Lian Feng , Hongling Xu , Liubin Xu , Yufeng Xing

{"title":"ROS-responsive injectable hydrogels loaded with exosomes carrying miR-4500 reverse liver fibrosis","authors":"Huili Yang , Wanshun Wang , Jiacong Xiao , Rong Yang , Lian Feng , Hongling Xu , Liubin Xu , Yufeng Xing","doi":"10.1016/j.biomaterials.2024.122887","DOIUrl":"10.1016/j.biomaterials.2024.122887","url":null,"abstract":"<div><div>The reversal of liver fibrosis requires effective strategies to reduce oxidative stress and inhibition of hepatic stellate cell (HSC) activation. MiR-4500 regulates pathological angiogenesis and collagen mRNA stability, with the potential to inhibit fibrosis. Herein, we explored the inhibition of HSC activation <em>in vitro</em> by exosomes (Exos) carrying miR-4500 and encapsulated Exos<sup>miR−4500</sup> in an intelligent injectable hydrogel with biological activity and reactive oxygen species (ROS) responsiveness for application in oxidative stress environments. Briefly, reversible boronic ester bonds were integrated into gelatin-based hydrogels through dynamic crosslinking of quaternized chitosan (QCS) and 4-carboxyphenylboronic acid (CPBA)-modified gelatin. The QCS-CPBA-Gelatin (QCG) hydrogel scavenged excess ROS from the local microenvironment and released Exos<sup>miR−4500</sup> through the dissociation of boronic ester bonds, providing a favorable microenvironment and <em>in situ</em> sustained-release drug delivery system for Exos<sup>miR−4500</sup>. The results showed that QCG@Exos<sup>miR−4500</sup> hydrogel has biocompatibility, biodegradability, and slow-release ability, which could effectively clear ROS and inhibit HSC activation and pathological angiogenesis <em>in vitro</em> and <em>in vivo</em>. Furthermore, transcriptome analysis suggests that the pharmacological mechanism of the QCG@Exos<sup>miR−4500</sup> hydrogel is mainly related to anti-oxidation, anti-angiogenesis, anti-fibrosis processes, and signaling pathways. Thus, our study demonstrates that an intelligently responsive Exos<sup>miR−4500</sup> delivery system based on injectable hydrogels is a promising strategy for the treatment of liver fibrosis.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"314 ","pages":"Article 122887"},"PeriodicalIF":12.8,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomimetic polymeric nanoreactors with photooxidation-initiated therapies and reinvigoration of antigen-dependent and antigen-free immunity 具有光氧化诱导疗法的仿生聚合物纳米反应器，重振抗原依赖性和无抗原性免疫力

IF 12.8 1区医学

Biomaterials Pub Date : 2024-10-10 DOI: 10.1016/j.biomaterials.2024.122884

Pu Qiu, Mei Wen, Zixuan Zhuang, Shining Niu, Cheng Tao, Nuo Yu, Zhigang Chen

{"title":"Biomimetic polymeric nanoreactors with photooxidation-initiated therapies and reinvigoration of antigen-dependent and antigen-free immunity","authors":"Pu Qiu, Mei Wen, Zixuan Zhuang, Shining Niu, Cheng Tao, Nuo Yu, Zhigang Chen","doi":"10.1016/j.biomaterials.2024.122884","DOIUrl":"10.1016/j.biomaterials.2024.122884","url":null,"abstract":"<div><div>Immune cell-mediated anticancer modalities usually suffer from immune cell exhaustion and limited efficacy in solid tumors. Herein, the oxygen-carrying biomimetic nanoreactors (BNR2(O<sub>2</sub>)) have been developed with photooxidation-driven therapies and antigen-dependent/antigen-free immune reinvigoration against xenograft tumors. The BNR2(O<sub>2</sub>) composes polymeric nanoreactors camouflaged with cancer cell membranes can efficiently target homotypic tumors. It continuously releases O<sub>2</sub> to boost intracellular reactive oxygen species (ROS) to oxide diselenide bonds, which controllably releases seleninic acids and anti-folate Pemetrexed compared to hydrogen peroxide and glutathione incubation. The O<sub>2</sub>-rich microenvironment sensitizes Pemetrexed and blocks programmed cell-death ligand 1 (PD-L1) to reverse T cell immunosuppression. The ROS and Pemetrexed upregulate pro-apoptosis proteins and inhibit folate-related enzymes, which cause significant apoptosis and immunogenic cell death to stimulate dendritic cell maturation for improved secretion of cytokines, expanding antigen-dependent T cell immunity. Furthermore, by regulating the release of seleninic acids, the checkpoint receptor human leukocyte antigen E of tumor cells can be blocked to reinvigorate antigen-free natural killer cell immunity. This work offers an advanced antitumor strategy by bridging biomimetic nanoreactors and modulation of multiple immune cells.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"314 ","pages":"Article 122884"},"PeriodicalIF":12.8,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142417474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrically-stimulated cellular and tissue events are coordinated through ion channel-mediated calcium influx and chromatin modifications across the cytosol-nucleus space 电刺激的细胞和组织事件通过离子通道介导的钙离子流入和细胞质-细胞核间隙的染色质修饰得到协调

IF 12.8 1区医学

Biomaterials Pub Date : 2024-10-09 DOI: 10.1016/j.biomaterials.2024.122854

Shanika Karunasagara , Buuvee Bayarkhangai , Hye-Won Shim , Han-Jin Bae , Hwalim Lee , Ali Taghizadeh , Yunseong Ji , Nandin Mandakhbayar , Hye Sung Kim , Jeongeun Hyun , Tae-Jin Kim , Jung-Hwan Lee , Hae-Won Kim

{"title":"Electrically-stimulated cellular and tissue events are coordinated through ion channel-mediated calcium influx and chromatin modifications across the cytosol-nucleus space","authors":"Shanika Karunasagara , Buuvee Bayarkhangai , Hye-Won Shim , Han-Jin Bae , Hwalim Lee , Ali Taghizadeh , Yunseong Ji , Nandin Mandakhbayar , Hye Sung Kim , Jeongeun Hyun , Tae-Jin Kim , Jung-Hwan Lee , Hae-Won Kim","doi":"10.1016/j.biomaterials.2024.122854","DOIUrl":"10.1016/j.biomaterials.2024.122854","url":null,"abstract":"<div><div>Electrical stimulation (ES) through biomaterials and devices has been implicated in activating diverse cell behaviors while facilitating tissue healing process. Despite its significance in modulating biological events, the mechanisms governing ES-activated cellular phenomena remain largely elusive. Here, we demonstrated that millisecond-pulsed temporal ES profoundly impacted a spectrum of cellular events across the membrane-cytosol-nuclear space. These include activated ion channels, intracellular calcium influx, actomyosin contractility, cell migration and proliferation, and secretome release. Such events were coordinated mainly through ES-activated ion channels and calcium oscillation dynamics. Notably, ES increased the chromatin accessibility of genes, particularly those associated with the ES-activated cellular events, underscoring the significance of epigenetic changes in ES-induced behavioral outcomes. We identified histone acetylation (mediated by histone acetyltransferases), among other chromatin modifications, is key in reshaping the chromatin landscape upon ES. These observations were further validated through experiments involving <em>ex vivo</em> skin tissue samples, including activated ion channels and calcium influx, increased cell proliferation and actomyosin contractility, elevated secretome profile, and more accessible chromatin structure following ES. This work provides novel insights into the mechanisms underlying ES-activated cell and tissue events, ultimately guiding design principles for the development of electrical devices and materials effective for tissue repair and wound healing.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"314 ","pages":"Article 122854"},"PeriodicalIF":12.8,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial-uncoupling nanomedicine for self-heating and immunometabolism regulation in cancer cells 用于调节癌细胞自热和免疫代谢的线粒体耦合纳米药物

IF 12.8 1区医学

Biomaterials Pub Date : 2024-10-09 DOI: 10.1016/j.biomaterials.2024.122883

Zhe Yang , Ying Zhou , Xiaozhen Liu , Liujiao Ren , Xinyang Liu , Rong Yun , Liangliang Jia , Xuechun Ren , Ying Wang , Yan Sun , Jia Li , Di Gao , Zhongmin Tian

{"title":"Mitochondrial-uncoupling nanomedicine for self-heating and immunometabolism regulation in cancer cells","authors":"Zhe Yang , Ying Zhou , Xiaozhen Liu , Liujiao Ren , Xinyang Liu , Rong Yun , Liangliang Jia , Xuechun Ren , Ying Wang , Yan Sun , Jia Li , Di Gao , Zhongmin Tian","doi":"10.1016/j.biomaterials.2024.122883","DOIUrl":"10.1016/j.biomaterials.2024.122883","url":null,"abstract":"<div><div>Developing endogenous hyperthermia offers a promising strategy to address challenges with current exogenous hyperthermia techniques in clinics. Herein, a CD44-targeted and thermal-responsive nanocarrier was developed for the simultaneous delivery of 2,4-dinitrophenol and syrosingopine. The objective was to induce endogenous hyperthermia and regulate immunometabolism, ultimately augmenting anti-tumour immune responses. Dinitrophenol as mitochondrial uncoupler can convert electrochemical potential energy of inner mitochondrial membrane into heat, facilitating endogenous hyperthermia. Meanwhile, syrosingopine not only inhibits excessive lactate efflux caused by dinitrophenol but also downregulates tumour cell glycolysis, thus alleviating immunosuppression and heat shock protein (HSP)-dependent thermo-resistance through immunometabolism regulation. The synergistic effects of endogenous hyperthermia and immunometabolism regulation by this nanomedicine have potential to enhance tumor immunogenicity, reshape the tumour immune microenvironment, and effectively suppress the growth of subcutaneous tumours and patient-derived organoids in triple-negative breast cancer. Therefore, the endogenous hyperthermia strategy developed in this study would revolutionize hyperthermia for cancer treatment.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"314 ","pages":"Article 122883"},"PeriodicalIF":12.8,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142417531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mesoporous cerium oxide nanoenzyme for Efficacious impeding tumor and metastasis via Conferring resistance to anoikis 介孔氧化铈纳米酶通过增强抗窒息性来有效抑制肿瘤和转移。

IF 12.8 1区医学

Biomaterials Pub Date : 2024-10-08 DOI: 10.1016/j.biomaterials.2024.122876

Yunhao Wang , Lei Ding , Juan Feng , Ziguo Lin , Hanlin Yao , Xinyu You , Xiaolong Zhang , Wen Sun , Yang Liu , Peiyuan Wang

{"title":"Mesoporous cerium oxide nanoenzyme for Efficacious impeding tumor and metastasis via Conferring resistance to anoikis","authors":"Yunhao Wang , Lei Ding , Juan Feng , Ziguo Lin , Hanlin Yao , Xinyu You , Xiaolong Zhang , Wen Sun , Yang Liu , Peiyuan Wang","doi":"10.1016/j.biomaterials.2024.122876","DOIUrl":"10.1016/j.biomaterials.2024.122876","url":null,"abstract":"<div><div>Tumor cells can survive when detached from the extracellular matrix or lose cell-to-cell connections, leading to a phenomenon known as anoikis resistance (AR). AR is closely associated with the metastasis and proliferation of tumor cells, enabling them to disseminate, migrate, and invade after detachment. Here, we have investigated a novel composite nanoenzyme comprising mesoporous silica/nano-cerium oxide (MSN-Ce@SP/PEG). This nanoenzyme exhibited satisfactory catalase (CAT) activity, efficiently converting high levels of H<sub>2</sub>O<sub>2</sub> within tumor cells into O<sub>2</sub>, effectively alleviating tumor hypoxia. Furthermore, MSN-Ce@SP/PEG nanoenzyme demonstrated high peroxidase (POD) activity, elevating reactive oxygen species (ROS) levels and attenuating AR in hepatocellular carcinoma (HCC) cells. The MSN-Ce@SP/PEG nanoenzyme exhibited satisfactory dual bioactivity in CAT and POD and was significantly enhanced under favorable photothermal conditions. Through the synergistic effects of these capabilities, the nanoenzyme disrupted the epithelial-mesenchymal transition (EMT) process in detached HCC cells, ultimately inhibiting the recurrence and metastasis potential of anoikis-resistant HCC cells. This study represents the first report of a novel nanoenzyme based on mesoporous silica/nano-cerium oxide for treating AR in HCC cells, thereby suppressing HCC recurrence and metastasis. The findings of this work offer a pioneering perspective for the development of innovative strategies to prevent the recurrence and metastasis of HCC.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"314 ","pages":"Article 122876"},"PeriodicalIF":12.8,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bifunctional mesoporous HMUiO-66-NH2 nanoparticles for bone remodeling and ROS scavenging in periodontitis therapy 双功能介孔 HMUiO-66-NH2 纳米粒子在牙周炎治疗中用于骨重塑和清除 ROS。

IF 12.8 1区医学

Biomaterials Pub Date : 2024-10-05 DOI: 10.1016/j.biomaterials.2024.122872

Waishan Lam , Yufei Yao , Chenxi Tang , Yue Wang , Quan Yuan , Lin Peng

{"title":"Bifunctional mesoporous HMUiO-66-NH2 nanoparticles for bone remodeling and ROS scavenging in periodontitis therapy","authors":"Waishan Lam , Yufei Yao , Chenxi Tang , Yue Wang , Quan Yuan , Lin Peng","doi":"10.1016/j.biomaterials.2024.122872","DOIUrl":"10.1016/j.biomaterials.2024.122872","url":null,"abstract":"<div><div>Periodontal bone defects represent an irreversible consequence of periodontitis associated with reactive oxygen species (ROS). However, indiscriminate removal of ROS proves to be counterproductive for tissue repair and insufficient for addressing existing bone defects. In the treatment of periodontitis, it is crucial to rationally alleviate local ROS while simultaneously promoting bone regeneration. In this study, Zr-based large-pore hierarchical mesoporous metal-organic framework (MOF) nanoparticles (NPs) HMUiO-66-NH<sub>2</sub> were successfully proposed as bifunctional nanomaterials for bone regeneration and ROS scavenging in periodontitis therapy. HMUiO-66-NH<sub>2</sub> NPs demonstrated outstanding biocompatibility both <em>in vitro</em> and <em>in vivo.</em> Significantly, these NPs enhanced the osteogenic differentiation of bone mesenchymal stem cells (BMSCs) under normal and high ROS conditions, upregulating osteogenic gene expression and mitigating oxidative stress. Furthermore, <em>in vivo</em> imaging revealed a gradual degradation of HMUiO-66-NH<sub>2</sub> NPs in periodontal tissues. Local injection of HMUiO-66-NH<sub>2</sub> effectively reduced bone defects and ROS levels in periodontitis-induced C57BL/6 mice. RNA sequencing highlighted that differentially expressed genes (DEGs) are predominantly involved in bone tissue development, with notable upregulation in Wnt and TGF-β signaling pathways. In conclusion, HMUiO-66-NH<sub>2</sub> exhibits dual functionality in alleviating oxidative stress and promoting bone repair, positioning it as an effective strategy against bone resorption in oxidative stress-related periodontitis.</div></div>","PeriodicalId":254,"journal":{"name":"Biomaterials","volume":"314 ","pages":"Article 122872"},"PeriodicalIF":12.8,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142386750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0