Arabian Journal of Chemistry最新文献

{"title":"Bimetallic FeCo metal–organic framework based cascade reaction system: Enhanced peroxidase activity for antibacterial performance","authors":"Siyuan Zhang ,&nbsp;Wenbo Wu ,&nbsp;Ya Li ,&nbsp;Dongxin Wang ,&nbsp;Xiangjun Li ,&nbsp;Jisheng Nie ,&nbsp;Hong Cui","doi":"10.1016/j.arabjc.2024.106032","DOIUrl":"10.1016/j.arabjc.2024.106032","url":null,"abstract":"<div><div>Metal-organic frameworks (MOFs), as a peroxidase (POD), can catalyze the conversion of H₂O₂ to reactive oxygen species (ROS) for antibacterial application. To achieve strong antibacterial activity, it is necessary to improve the enzyme-like activity of MOFs. In this study, Fe_xCoMOF was synthesized by incorporating Co(II) to improve the electron transfer of Fe(III)/Fe(II), which enhanced its redox capacity as a nanozyme and further promoted the generation of hydroxyl radical (⋅OH), exhibiting higher POD activity. Doping with different amounts of Co(II) altered the particle size, specific surface area, and surface defects of Fe_xCoMOF, thus exhibiting differential enzyme-like activities. Additionally, a glucose-responsive enzyme cascade reaction system based on Fe₄CoMOF/glucose oxidase (GOx) was established. In the presence of glucose, the <em>in situ</em>-generated substrate H₂O₂ was in contact with the catalytic site and the generated gluconic acid enabled Fe₄CoMOF to maintain maximum enzyme activity under physiological pH conditions, avoiding damage caused by the use of exogenous high concentrations of H₂O₂. In the <em>in vitro</em> antibacterial experiment, the minimum inhibitory concentration (MIC) of Fe₄CoMOF/GOx was 10 μg mL⁻¹ for <em>Escherichia coli</em> (<em>E. coli</em>) and 5 μg mL⁻¹ for <em>Staphylococcus aureus</em> (<em>S. aureus</em>) (∼10⁸ CFU mL⁻¹). The increase in enzyme activity resulted in a considerable reduction in the dose of the antibacterial agent, which was conducive to improving bio-safety. The <em>in vivo</em> experiment in mice demonstrated that the glucose-responsive Fe₄CoMOF-based cascade reaction system had excellent antibacterial properties and remarkably promoted wound healing. The antibacterial agent based on bimetallic MOF developed in this work provides a new idea for cascade catalytic antibacterial therapy and will have remarkable application prospects in biomaterials and nanomedicine.</div></div>","PeriodicalId":249,"journal":{"name":"Arabian Journal of Chemistry","volume":"17 12","pages":"Article 106032"},"PeriodicalIF":5.3,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of dimethachlon metabolites and dissipation behavior, processing factor and risk assessment of dimethachlon in rapeseed","authors":"Lianhong Mou ,&nbsp;Ling Wu ,&nbsp;Ling Liu,&nbsp;Yu Xiang,&nbsp;Deyu Hu,&nbsp;Yuping Zhang","doi":"10.1016/j.arabjc.2024.106030","DOIUrl":"10.1016/j.arabjc.2024.106030","url":null,"abstract":"<div><div>Pesticides and their transformation products are found in raw agricultural and processed food products, posing risks to human health. Therefore, a thorough investigation of dimethachlon’s dissipation, metabolites, residue levels in crops, and changes during food processing is essential for an accurate dietary risk assessment. This study identified two metabolites, 4-(3,5-dichloroanilino)-4- oxobutanoic acid (DCBAA) and 3,5-dichloroaniline (3,5-DCA), formed from dimethachlon in crops under field conditions. A precise and sensitive analytical method was developed to detect dimethachlon, DCBAA, and 3,5-DCA in rapeseed pods, seeds, and oil. The half-lives of dimethachlon in rapeseed pods at two sites ranged from 1.61 to 1.67 days. During degradation, the maximum residue levels of DCBAA and 3,5-DCA in rapeseed pods reached 38.59 % and 3.47 % of the initial parent compound concentration, respectively. At harvest (day 10), final dimethachlon residues in rapeseed were 0.11 mg/kg and 0.27 mg/kg. Processing rapeseed into oil through sun-drying and stir-frying reduced dimethachlon residues, with processing factors (PF) of 0.69 and 0.64, respectively. However, pressing the seeds concentrated dimethachlon in the oil, with a PF of 1.92. Notably, DCBAA tended to concentrate during sun-drying but decreased during stir-frying and pressing. Supervised trials indicated that dimethachlon residues in rapeseed posed no long-term dietary risks.</div></div>","PeriodicalId":249,"journal":{"name":"Arabian Journal of Chemistry","volume":"17 12","pages":"Article 106030"},"PeriodicalIF":5.3,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organocatalyst as a synthetic gadget for pharmaceutically potent molecules","authors":"Labiqa Aman ,&nbsp;Shehla Khalid ,&nbsp;Nasir Rasool ,&nbsp;Almeera Zia ,&nbsp;Muhammad Imran ,&nbsp;Marius Irimie ,&nbsp;Codrut Ioan Ciurea","doi":"10.1016/j.arabjc.2024.106027","DOIUrl":"10.1016/j.arabjc.2024.106027","url":null,"abstract":"<div><div>Organocatalysts have become a third main method of catalyzing chemical reactions aside from bio and metal catalysts. The potential for green chemistry and some striking benefits, including stability, low cost, easy availability, excellent enantioselectivity, and simple recovery, have made organocatalyzed reactions a fruitful approach. Recent developments in the field of organocatalysis include the enhancement of enantioselectivity, the development of novel catalyst classes such as NHCs, and dual catalysis, which combines organo- with metal or photocatalysis to enable novel reactivity. This review focuses on organocatalyzed chemical synthesis of pharmacophores such as benzoxazinones, pyrrolidines, triazoles, pyrazolinones, tricyclic coumarins, (+)-paroxetine, and (+)-femoxetine. The review has been outlined into six categories i.e. Lewis base catalysts, Lewis acid catalysts, Bronsted base catalysts, Bronsted acid catalysts, bifunctional catalysts, and organo-photocatalysts. Less reusability due to catalyst deactivation demands higher catalytic loading. Thus scalability in this field is still challenging. Moreover, achieving higher catalytic efficiency needs future efforts.</div></div>","PeriodicalId":249,"journal":{"name":"Arabian Journal of Chemistry","volume":"17 12","pages":"Article 106027"},"PeriodicalIF":5.3,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green chemistry-based synthesis of gold nanoparticles using Nicotiana plumbaginifolia and their anticancer properties against cervical cancer","authors":"Xiaorong Yang ,&nbsp;Yin Bao ,&nbsp;Xia Zhou ,&nbsp;Hong Zhu ,&nbsp;Jun Gao","doi":"10.1016/j.arabjc.2024.106029","DOIUrl":"10.1016/j.arabjc.2024.106029","url":null,"abstract":"<div><div>Although various strategies have been reported for the synthesis of gold nanoparticles (AuNPs) for possible anticancer effects, green synthesis can be considered an efficient strategy for the production of efficient NPs with minimal toxic effects. In this study, we investigate the green synthesis of gold nanoparticles (AuNPs) using an aqueous leaf extract of <em>Nicotiana plumbaginifolia</em> and evaluated their protein/DNA binding, hemolysis characteristics, and anticancer properties against HeLa cervical cancer cells, while normal HUVECs were used as the control sample. Different techniques were utilized to characterize the development of AuNPs. Then, the interaction properties of biosynthesized AuNPs with HeLa cancer cells and HUVECs were assessed by cellular assays. The results showed that the Au nanosphere had an average particle size of 18 nm with four distinctive X-ray diffraction (XRD) peaks at 38.00°, 44.10°, 63.75° and 77.10°. Moreover, the UV–Vis spectrum showed a maximum absorption peak at about 538 nm, corresponding to the surface Plasmon resonance (SPR) characteristics of the AuNPs. Furthermore, an observed Fourier-transform infrared spectroscopy (FTIR) peak at about 769 cm⁻¹ was attributed to the Au<img>O stretching vibration. Dynamic light scattering (DLS) analysis also showed that the maximum size and zeta potential values for colloid AuNPs were 68.69 nm and −47.92 mV, respectively. Then, it was detected that after incubation of maximum % solution of AuNPs, 0.1, % BSA and %DNA binding values were 58.76 % and 32.15 %, respectively. It was also determined that following incubation of red blood cells (RBC) with maximum concentrations of AuNPs, 20 μM, the hemolysis value was 2.29 %. Cellular assays showed that the IC₅₀ value of AuNPs was 1.53 μM in HeLa cells, whereas this value for HUVEC normal cells was 30.55 μM. It was also shown that incubation of cells with AuNPs for 24 h led to a higher upregulation in the levels of released Cytc. C, caspase-9, and caspase-3 in HeLa cancer cells compared with HUVECs. Then, it was determined that stimulation of ROS generation and subsequent apoptosis, upregulation of Bax/Bcl-2, caspase-9, and caspase-3 mRNA, induced by AuNPs can be reversed by pretreatment of cells with NAC, a potential antioxidant. Finally, it was demonstrated that although the exposure of HeLa cells to AuNPs after 24 h caused a significant reduction in cell viability mediated by apoptosis, pre-treatment of cells with caspase-9 and caspase-3 inhibitors, significantly mitigated apoptosis induction and recovered cell viability. In conclusion, this paper might provide useful information about the medicinal purposes enabled by AuNPs.</div></div>","PeriodicalId":249,"journal":{"name":"Arabian Journal of Chemistry","volume":"17 12","pages":"Article 106029"},"PeriodicalIF":5.3,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142552994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical sensing of gatifloxacin using Ag2S/RGO nanocomposite","authors":"Chunxia Yao ,&nbsp;Ying Liang ,&nbsp;Sai Huang ,&nbsp;Chengbin Liu ,&nbsp;Wei Song ,&nbsp;Weiguo Song","doi":"10.1016/j.arabjc.2024.106031","DOIUrl":"10.1016/j.arabjc.2024.106031","url":null,"abstract":"<div><div>As a widely-used fluoroquinolone antibiotics, 1-Cyclopropyl-6-fluoro-8-methoxy-7-(3-methyl-1-piperazinyl)-4-oxo-1,4-dihydro-3-quinolinecarboxylic acid (gatifloxacin, GAT) has aroused much concern recently and it is of great importance to realize the accurate and efficient detection. Herein, the silver sulfide/reduced graphene oxide composite (Ag₂S/RGO) was synthesized with one-pot method and was coated on the glassy carbon electrode to develop an effective electrochemical sensor for GAT. After the optimization of the size of Ag₂S, the pH of buffer solution, and the scanning rate, the Ag₂S/RGO modified electrode exhibits good linear relationship to GAT within wide ranges of 0.2 µM − 20 µM and 20 µM − 250 µM with a detection limit of 0.0667 µM. Besides, the proposed sensor shows good selectivity to GAT versus multiple interferences, including organic compounds, ions, and other antibiotics. At last, the proposed sensor was successfully applied in the GAT analysis in various real samples (including shrimp, fish, and chicken) with satisfying recoveries of 91.8 % − 102.4 %. In general, the proposed Ag₂S/RGO-based electrochemical sensor provides a novel strategy for the GAT analysis, which is of significance for the development of efficient analytical techniques for antibiotics.</div></div>","PeriodicalId":249,"journal":{"name":"Arabian Journal of Chemistry","volume":"17 12","pages":"Article 106031"},"PeriodicalIF":5.3,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142534391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hansen parameters and GastroPlus assisted optimized topical elastic liposomes to treat breast cancer using a novel isatin derivative","authors":"Mohammad A. Altamimi ,&nbsp;Afzal Hussain ,&nbsp;Mohammed M. Alanazi ,&nbsp;Dhafer Alotaibi ,&nbsp;Saeed Ali Syed ,&nbsp;Ahmed Bari","doi":"10.1016/j.arabjc.2024.106028","DOIUrl":"10.1016/j.arabjc.2024.106028","url":null,"abstract":"<div><div>Breast cancer treatment is a global health challenge using conventional toxic chemotherapeutic agents. A novel isatin could be a promising alternative. An isatin derivative (TM-19) was synthesized and characterized using NMR (nuclear magnetic resonance), mass spectrometry, Fourier transform infrared (FTIR), and differential scanning calorimetry (DSC). HSPiP and GastroPlus predictive software were used to predict suitable solvents for solubility and in vivo oral pharmacokinetics in humans (fasted condition), respectively. Based on predictive findings, topical elastic liposomes (phosphatidylcholine as PC and span 80 by film hydration method) was prepared, characterized, and optimized using QbD (quality by design). QbD identified the impact of composition on response variables (Y₁ for size, Y₂ for polydispersity index, Y₃ for zeta potential, and Y₄ for %EE). In vitro cytotoxicity study was carried out against MCF-7 cell lines. Low molecular weight and lipophilic TM-19 was crystalline in nature (255 g/mol). GastroPlus program predicted limited in vivo dissolution and poor oral absorption (10.4 %). Results confirmed the highest TM-19 solubility in DMSO (dimethyl sulfoxide) ˃ chloroform ˃ PEG 400 (polyethylene glycol 400). Considering experimental and predicted data, topical delivery of TM-19 using optimized (desirability parameter value = 0.97) elastic liposomes (TF1) was quite promising due to optimal size (344 nm), high %EE (61.7 %), low PDI (0.45), and optimal zeta potential (− 12.34 mV). TM-19 loaded TF1 elicited concentration dependent cytotoxic effect (˃ 90 % at 10 µM) against MCF-7 as compared to TM-19 suspension.</div></div>","PeriodicalId":249,"journal":{"name":"Arabian Journal of Chemistry","volume":"17 12","pages":"Article 106028"},"PeriodicalIF":5.3,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142571863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-depth review of synthesis of hydroxyapatite biomaterials from natural resources and chemical regents for biomedical applications","authors":"Precious Osayamen Etinosa ,&nbsp;Obinna Anayo Osuchukwu ,&nbsp;Emeka Obiora Anisiji ,&nbsp;Mohammed Y. Lawal ,&nbsp;Sikiru Adepoju Mohammed ,&nbsp;Opeyemi Isaac Ibitoye ,&nbsp;Peter Gbenga Oni ,&nbsp;Victor D. Aderibigbe ,&nbsp;Toyin Aina ,&nbsp;Damilola Oyebode ,&nbsp;Solomon C. Nwigbo","doi":"10.1016/j.arabjc.2024.106010","DOIUrl":"10.1016/j.arabjc.2024.106010","url":null,"abstract":"<div><div>Hydroxyapatite (HAp) is a popular ceramic biomaterial, which has extensively been employed in numerous biomedical applications. It has drawn the attention of scientists primarily owing to its properties similar to the bones and teeth of human beings. Due to its organic similarity, HAp has exceptional biocompatibility and can activate osteoconduction and osteoinduction occurrence. Hence, HAp has been used in a broad collection of healing applications extending from common dental implant, and bioactive coatings on grafts to purposefully premeditated drug delivery methods, and even for tissue engineering (TE) applications. The production method is essential in controlling the physicochemical properties of HAp, which in turn affects its mechanical and biological properties. The anticipated physiognomies of HAp can be accomplished using a suitable technique of production and sources. This in-depth review discussed the various natural sources of HAp, and synthesis techniques for producing HAp, alongside their advantages and disadvantages, applications, and essentials of HAp for therapeutic applications. From the review, it was found that the sol–gel route is preferred over other routes because of its even molecular mixing, high purity of products, low production temperature, and ability to synthesize nanosized particles of HAp. As a result, it is provisional that upon the cautious blend of key considerations such as sources, synthesis technique, and production parameters for the manufacture of natural HAp (NHAp), mechanical properties can be improved and the product can be employed as a drug delivery system. These are important extents for the prospect of the research.</div></div>","PeriodicalId":249,"journal":{"name":"Arabian Journal of Chemistry","volume":"17 12","pages":"Article 106010"},"PeriodicalIF":5.3,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142571864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo wound healing activity of electrospun nanofibers embedding natural products","authors":"Breno de Almeida Bertassoni ,&nbsp;Denise de Abreu Garófalo ,&nbsp;Mariana Sato de Souza Bustamante Monteiro ,&nbsp;Ralph Santos-Oliveira ,&nbsp;Anne Caroline Candido Gomes ,&nbsp;Anna Lecticia Martinez Martinez Toledo ,&nbsp;Marcos Lopes Dias ,&nbsp;Naomi Kato Simas ,&nbsp;Eduardo Ricci-Junior","doi":"10.1016/j.arabjc.2024.106019","DOIUrl":"10.1016/j.arabjc.2024.106019","url":null,"abstract":"<div><div>Nanofibers are threads at the nanometric scale. Electrospinning is a technique that uses electric fields as the driving force to produce fibers ranging from nanometers to micrometers. Membranes based on polymeric nanofibers obtained through electrospinning possess an excellent capacity to carry drugs and natural active components, promoting skin healing and regeneration in various ways. Natural products derived from plants have gained attention in recent decades due to their accessibility, good biocompatibility, biodegradability, and incorporation into nanofibers. These products can influence multiple stages of the healing process and exhibit antimicrobial activity, thereby preventing and combating infections. Consequently, the combination of natural products and nanofibers presents a promising approach to infection prevention and skin wound healing. In this context, the current paper presents a systematic literature review of studies conducted from 2011 to 2024. It focuses on articles concerning the production of nanofibers via electrospinning that embed active components of natural origin, specifically those that performed <em>in vivo</em> tests to assess wound healing potential. A total of 26<!--> <!-->articles met the inclusion criteria and were analyzed in terms of production and characterization of the electrospun membranes using <em>in<!--> <!-->vitro</em> and <em>in vivo</em> tests. Most studies employed a mixture of polyvinyl alcohol (PVA) with another polymer, utilized plant extracts like <em>Malva sylvestris</em> as active components, and performed <em>in<!--> <!-->vitro</em> tests using fibroblasts. Antimicrobial tests were conducted against <em>Staphylococcus aureus</em> and <em>Escherichia coli,</em> while male Wistar rats were used in excision/incision wound models for <em>in<!--> <!-->vivo</em> tests. The results indicate that natural products influence the properties of the scaffolds in different ways, such as increasing fiber diameter and mechanical strength, and may also accelerate wound healing.</div></div>","PeriodicalId":249,"journal":{"name":"Arabian Journal of Chemistry","volume":"17 12","pages":"Article 106019"},"PeriodicalIF":5.3,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of micropatterning with nanowire-based microcavity array on bacterial enrichment and selective distribution","authors":"Yifan Wang ,&nbsp;Yuting Huang ,&nbsp;Hui Che ,&nbsp;Biaowen Wei ,&nbsp;Liyuan Zheng","doi":"10.1016/j.arabjc.2024.106013","DOIUrl":"10.1016/j.arabjc.2024.106013","url":null,"abstract":"<div><div>The micro-patterned topography can affect bacterial enrichment and selective distribution by modifying the characteristics of material surface. In this study, we fabricated microcavity patterned silicon surfaces, and quantitatively explored the amount and distribution of Escherichia coli (<em>E. coli</em>) cells attached on a series of defined topographies. The results showed that <em>E. coli</em> cells enrichment was significantly increased on silicon nanowires-based microcavity array when compared to nanowires patterned silicon wafer. Furthermore, the microcavity diameter can control bacterial distribution by changing hydrophilic performance of interface. With the microcavity diameter of 7 µm, the cells colonized into the microcavity almost in its entirety, whilst distributed around the microcavity completely with 10 µm diameter, regardless of centre distances. This phenomenon can be explained by the Cassie-Baxter model equation according to the wettability. The distribution of bacterial enrichment changed with the silicon surfaces turning from non-wetting status to wetting status when treated with oxygen plasma to modify the hydrophobicity. These results demonstrated that microcavity patterned surface could favor bacterial enrichment on silicon and strictly confined bacterial distribution inside/outside the microcavity. Moreover, the nanowires inside microcavity can also increase electron conductivity and reduce the internal resistance, thus providing scientific evidence for the design of wearable microbial fuel cell with rational optimization and integration of different components for electronic skin.</div></div>","PeriodicalId":249,"journal":{"name":"Arabian Journal of Chemistry","volume":"17 12","pages":"Article 106013"},"PeriodicalIF":5.3,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical composition and anticancer effect of Akebia trifoliata seed in non-small cell lung cancer A549 cells","authors":"Yuanquan Ran ,&nbsp;Lanlan Yang ,&nbsp;Xiaoyan Jia ,&nbsp;Huan Zhao ,&nbsp;Qiong Hu ,&nbsp;Bing Yang ,&nbsp;Dongxin Tang ,&nbsp;Minyi Tian","doi":"10.1016/j.arabjc.2024.106020","DOIUrl":"10.1016/j.arabjc.2024.106020","url":null,"abstract":"<div><h3>Background</h3><div><em>Akebia trifoliata</em> is a widely distributed medicine and food homology plant. Its fruit is used to treat tumors and the seed’s weight proportion exceeds 50%. However, the anti-tumor effect of <em>A. trifoliata</em> seed remains poorly studied.</div></div><div><h3>Methods</h3><div>UHPLC-Q-Orbitrap-MS was used to identify the chemical components of <em>A. trifoliata</em> seed water extract (WE) and ethanolic extract (EE), and their cytotoxicity was evaluated using MTT assay. Further, colony formation and cell cycle assays examined the anti-proliferative effect of <em>A. trifoliata</em> seed EE. Next, morphology observation, AO/EB staining, Hoechst 33,258 staining, Annexin V-PE/7-AAD staining, and JC-1 assays were employed to detect apoptosis. The influence of <em>A. trifoliata</em> seed EE on A549 cell metastasis was assessed by wound healing and transwell invasion tests. In addition, western blotting and network pharmacology were applied to further analyze its anticancer mechanism.</div></div><div><h3>Results</h3><div>Eighty-two components were characterized from the <em>A. trifoliata</em> seed WE and EE. For anticancer activity, <em>A. trifoliata</em> seed EE exhibited high cytotoxicity against cancer cells A549 (IC₅₀: 52.38 ± 1.04 μg/mL) and NCI-H1299 (IC₅₀: 70.29 ± 0.58 μg/mL) and revealed low cytotoxicity against non-cancer cells MRC-5 (IC₅₀: 101.28 ± 2.84 μg/mL) and L929 (IC₅₀: 113.44 ± 0.55 μg/mL). <em>A. trifoliata</em> seed EE suppressed A549 cell proliferation by inducing S-phase arrest <em>via</em> down-regulation of CDK2 and p21 as well as up-regulation of cyclin E1 and E2. It increased the Bax/Bcl-2 ratio, reduced mitochondrial membrane potential (ΔΨm), activated caspase-9 and caspase-3, cleaved PARP, and triggered apoptosis through the mitochondrial pathway. Besides, it repressed migratory and invasive ability by reducing MMP-2, MMP-9, and N-cadherin levels.</div></div><div><h3>Conclusion</h3><div><em>A. trifoliata</em> seed EE exhibits outstanding anticancer properties and has the exploitation potential as an anticancer drug in the pharmaceutical industry.</div></div>","PeriodicalId":249,"journal":{"name":"Arabian Journal of Chemistry","volume":"17 12","pages":"Article 106020"},"PeriodicalIF":5.3,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}