BULLETIN of the L.N. Gumilyov Eurasian National University. BIOSCIENCE Series最新文献_第5页

Features of the development of juvenile Aspius aspius (Linnaeus, 1758) from diﬀerent biotopes of the Ile river delta 伊莱河三角洲不同生物群落幼鱼的发育特征(Linnaeus, 1758

BULLETIN of the L.N. Gumilyov Eurasian National University. BIOSCIENCE Series Pub Date : 1900-01-01 DOI: 10.32523/2616-7034-2019-126-1-66-76

Н.Ш. Мамилов, Алматы Қазақстан Әл-Фараби атындағы Қазақ Ұлттық Университетi, Ф.Т. Амирбекова, Т.М. Шалахметова, Ж.А. Адильбаев, Т. Г. Конысбаев, Л.Р. Сутуева

引用次数: 0

Development of a protocol for determining the specific adulteration of food products by "real-time" polymerase chain reaction 制定通过“实时”聚合酶链反应确定食品特定掺假的方案

BULLETIN of the L.N. Gumilyov Eurasian National University. BIOSCIENCE Series Pub Date : 1900-01-01 DOI: 10.32523/2616-7034-2022-140-3-60-75

G.K. Abitaуeva, A.K. Shagirova, M.R. Toleubekova, N. Kushcheva, A. Abeev, Z. S. Sarmurzina

{"title":"Development of a protocol for determining the specific adulteration of food products by \"real-time\" polymerase chain reaction","authors":"G.K. Abitaуeva, A.K. Shagirova, M.R. Toleubekova, N. Kushcheva, A. Abeev, Z. S. Sarmurzina","doi":"10.32523/2616-7034-2022-140-3-60-75","DOIUrl":"https://doi.org/10.32523/2616-7034-2022-140-3-60-75","url":null,"abstract":"Adulteration of meat products is becoming a serious problem all over the world, including inappropriate labeling, prohibited additives, or replacement of expensive ingredients with cheaper components. The consequences of such manipulations can be dangerous for the health of a consumer. For example, it may cause food allergies and poisoning. In this article, there was developed a protocol for the qualitative determination of the DNA of the mitochondrial genome of chicken (Gallus gallus) by the polymerase chain reaction in real-time in raw and heat-treated meat products. The study was carried out to determine mixtures of meat and meat products from chicken, turkey, horse, lamb, pork, and beef. There were prepared 11 samples of 9 types of meat and two types of plants for the study of falsification. DNA isolation kits PrepMan Ultra (Thermo Fisher, USA) were used to obtain DNA with a high concentration between 1,900 ng/ml and 140 ng/ml. The selection of special primers and fluorescent probes was based on the alignment of the sequence of the chicken mitochondrial gene (Gallus gallus) from GenBank. All circuits are aligned using Primer Designer 3.0 software and synthesized by ThermoFisher. The authors have developed a protocol to determine the specific falsification of food products by a polymerase chain reaction in \"real-time\" mode. The PCR protocol was tested on a collection of DNA samples to determine specificity, and PCR with different DNA content was delivered to determine sensitivity. Chicken DNA was clearly traced in the DNA matrix in the range of 0.01-10%. Studies have shown that the developed protocol is a highly sensitive and specific express method for determining chicken DNA by a polymerase chain reaction in \"real-time\" mode, which allows them to be used in the diagnosis of animal DNA detection.","PeriodicalId":248021,"journal":{"name":"BULLETIN of the L.N. Gumilyov Eurasian National University. BIOSCIENCE Series","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131758811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Сonstruction of biological fertilizer based on nitrogen-fixing and phosphate-mobilizing bacteria 以固氮和磷化菌为基础的生物肥料Сonstruction

BULLETIN of the L.N. Gumilyov Eurasian National University. BIOSCIENCE Series Pub Date : 1900-01-01 DOI: 10.32523/2616-7034-2020-130-1-14-22

E. Z. Zhakupov, I. Puntus, A. Mukhamedzhanov, B. Ergazy, A.G. Mertassov

引用次数: 0

Exosomes and the role of exosomal miRNA in the diagnosis of lung cancer 外泌体及其miRNA在肺癌诊断中的作用

BULLETIN of the L.N. Gumilyov Eurasian National University. BIOSCIENCE Series Pub Date : 1900-01-01 DOI: 10.32523/2616-7034-2021-136-3-51-63

R. I. Bersimbaev, O. Bulgakova, A. Aripova, A. Kausbekova, G. A. Toxobayeva, A. Kussainova

引用次数: 0

Biodiversity of the meadow floristic complex of the ridge Ketpen-Temerlik Ketpen-Temerlik山脊草甸区系复合体的生物多样性

BULLETIN of the L.N. Gumilyov Eurasian National University. BIOSCIENCE Series Pub Date : 1900-01-01 DOI: 10.32523/2616-7034-2021-136-3-13-25

G. Sadyrova, T. Bazarbaeva, D. Bayzhigitov, S. Jamilova

{"title":"Biodiversity of the meadow floristic complex of the ridge Ketpen-Temerlik","authors":"G. Sadyrova, T. Bazarbaeva, D. Bayzhigitov, S. Jamilova","doi":"10.32523/2616-7034-2021-136-3-13-25","DOIUrl":"https://doi.org/10.32523/2616-7034-2021-136-3-13-25","url":null,"abstract":"This article presents results of long-term studies of the meadow flora of the Ketpen-Temerlik ridge within Kazakhstan and China. The article presents an analysis of the meadow floristic complex of the Ketpen-Temerlik ridge. The taxonomic diversity of species composition of meadow floristic complex, biomorphological analysis of life forms of meadow species, and also the belonging of meadow species to different geographical types of areas. A detailed floristic analysis of meadow flora made it possible to identify 624 species of vascular plants belonging to 230 genera and 47 families. According to the main taxonomic groups of plants, the meadow flora is represented by the Magnoliophyta department, which accounts for 82.2% of the total species composition, and only an insignificant percentage is in the Liliopsida department, 17.1%. The ratio of monocotyledonous and dicotyledonous plants in meadow flora is 1: 4.8. The total number of monocotyledons covers 107 species or 17.1% of the total number of species, there are 517 species of dicotyledonous plants or 82.2%. Analysis of the largest families of meadow flora made it possible to identify 16 largest families by the largest number of species, which contain 504 species. Analysis of the species richness of the genera of flora are: Potentilla, Carex, Ranunculus, Taraxacum, Astragalus, Silene, Veronica. The leading genus of meadow flora accounts for 25.1%. The article considers a boreal nature of the meadow flora. The article reveals a large presence among the meadow flora of Ketpen-Temerlik of mountainous middle asian species with an area limited to the territories of Mountainous Middle Asia.","PeriodicalId":248021,"journal":{"name":"BULLETIN of the L.N. Gumilyov Eurasian National University. BIOSCIENCE Series","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134570906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

К изучению паукообразных (Arachnida) – энтомофагов чешуекрылых вредителей яблони юго-востока Казахстана

BULLETIN of the L.N. Gumilyov Eurasian National University. BIOSCIENCE Series Pub Date : 1900-01-01 DOI: 10.32523/2616-7034-2023-142-1-41-56

I. I. Temreshev, B. K. Kopzhasarov, Z. B. Beknazarova, A.M. Sarbasova, A.Sh. Dzhanbatyrov

{"title":"К изучению паукообразных (Arachnida) – энтомофагов чешуекрылых вредителей яблони юго-востока Казахстана","authors":"I. I. Temreshev, B. K. Kopzhasarov, Z. B. Beknazarova, A.M. Sarbasova, A.Sh. Dzhanbatyrov","doi":"10.32523/2616-7034-2023-142-1-41-56","DOIUrl":"https://doi.org/10.32523/2616-7034-2023-142-1-41-56","url":null,"abstract":"Nowadays, 81 species and 56 genera of arachnids belonging to 4 orders (Pseudoscorpionida, Trombidiformes, Opiliones and Aranei) and 19 families have been recorded in apple orchards of southeastern Kazakhstan. The order of spiders (Aranei) is dominant in diversity – 16 families, 54 genera and 73 species, the remaining orders include one family and 1-2 genera and species. According to the number of genera and species, the Araneidae family is dominant – 11 species and 9 genera. It is followed by the families Thomisidae (9 genera and 10 species), Salticidae (8 genera and 9 species), Lycosidae (5 genera and 9 species), Philodromidae (4 genera and 9 species), Theridiidae (3 genera and 5 species) and Gnaphosidae (4 genera and 4 species). Other families are represented by 1-2 genera and species. In general, the fauna of arachnid apple plantations, in which chemical pesticide treatments are used, is much more depleted (by 2 or more times) compared to gardens without such treatments and natural biotopes. Data on the consumption of pest caterpillars by different types of spiders in laboratory maintenance are given. Amaurobius erberi (Keyserling, 1863) ate up to 10 caterpillars of the codling moth Cydia pomonella (Linnaeus, 1758) or up to 5 caterpillars of the clouded drab Orthosia incerta (Hufnagel, 1766) per day. Steatoda paykulliana (Walckenaer, 1806) destroyed an average of 5 caterpillars of the codling moth or 3 caterpillars of the clouded drab per day. Smaller species, such as Diaea suspiciosa O.P.-Cambridge, 1885, Xysticus pseudocristatus Azarkina & Logunov, 2001 and Evarcha arcuata (Clerck, 1758), ate 2-3 caterpillars per day. For the first time in the apple orchard, the spider Olios sericeus (Kroneberg, 1875) was recorded, in Kazakhstan this species was previously noted as synanthropic.","PeriodicalId":248021,"journal":{"name":"BULLETIN of the L.N. Gumilyov Eurasian National University. BIOSCIENCE Series","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129625242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of the HSP17.6 protein mechanism in BBSV infection HSP17.6蛋白在乙型肝炎病毒感染中的作用机制分析

BULLETIN of the L.N. Gumilyov Eurasian National University. BIOSCIENCE Series Pub Date : 1900-01-01 DOI: 10.32523/2616-7034-2021-135-2-38-47

Zh. Kozhabek, J.L. Үu, X. Wang

{"title":"Analysis of the HSP17.6 protein mechanism in BBSV infection","authors":"Zh. Kozhabek, J.L. Үu, X. Wang","doi":"10.32523/2616-7034-2021-135-2-38-47","DOIUrl":"https://doi.org/10.32523/2616-7034-2021-135-2-38-47","url":null,"abstract":"Beet black scorch virus (BBSV) has been reported as a natural pathogen of sugar beet and distributed all over the world, causing great economic losses to the sugar industry. Research on interactions between BBSV and its host by using model plant Nicotiana benthamiana is significantly important and nessesary for understanding virus infection process and exploring plant resistance mechanism. The results of sequencing the transcriptome of N. benthamiana infected with BBSV as well as gene screening in response to viral infection revealed upregulation of the small heat shock protein 17.6 gene (NbHSP17.6) and the effect of the protein on resistance to the virus. To further examine the involvement of HSP17.6 in defense responses in N. benthamiana, we tested interaction between HSP17.6 and other heat shock proteins such as HSP70 and HSP90 as well as BBSV encoded proteins. The results showed that HSP17.6 interacted with HSP70 and HSP90 but not with BBSV encoded proteins. When combined with other available results, it is possible that HSP17.6 acted as a small molecular chaperone to facilitate proper refolding of the specific proteins HSP70 and HSP90 required for BBSV infection and/or replication.","PeriodicalId":248021,"journal":{"name":"BULLETIN of the L.N. Gumilyov Eurasian National University. BIOSCIENCE Series","volume":"112 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133570754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphofunctional and psycho-physiological indicators of teachers 教师的形态功能和心理生理指标

BULLETIN of the L.N. Gumilyov Eurasian National University. BIOSCIENCE Series Pub Date : 1900-01-01 DOI: 10.32523/2616-7034-2019-127-2-58-65

G. Ulekeshova, A. Dinmukhamedova

引用次数: 0

The research of the antibacterial activity of yeast Saccharomyces cerevisiae var. boulardii Az-12 酿酒酵母菌布氏变种Az-12的抑菌活性研究

BULLETIN of the L.N. Gumilyov Eurasian National University. BIOSCIENCE Series Pub Date : 1900-01-01 DOI: 10.32523/2616-7034-2019-128-3-80-89

A. Saparbekova, A. Latif, Z.R. Ahmedova

引用次数: 0

Features of the association of essential oil Artemisia schrenkiana Ledeb plant growing in southeast Kazakhstan 生长在哈萨克斯坦东南部的沙蒿(Artemisia schrenkiana Ledeb)植物精油的关联特征

BULLETIN of the L.N. Gumilyov Eurasian National University. BIOSCIENCE Series Pub Date : 1900-01-01 DOI: 10.32523/2616-7034-2022-141-4-24-36

A. Ydyrys, M.T. Massimzhan, N. Abdolla, Z. Abdrassulova, S. Syraiyl

{"title":"Features of the association of essential oil Artemisia schrenkiana Ledeb plant growing in southeast Kazakhstan","authors":"A. Ydyrys, M.T. Massimzhan, N. Abdolla, Z. Abdrassulova, S. Syraiyl","doi":"10.32523/2616-7034-2022-141-4-24-36","DOIUrl":"https://doi.org/10.32523/2616-7034-2022-141-4-24-36","url":null,"abstract":"The article describes the features of the plant community of the territory where the plant Artemisia schrenkiana Ledeb, which grows in the Urzhar District of Abai Kazakhstan region, is found. The research work was carried out by Universal geobotanical methods. As a result of the study, the authors, with a comprehensive analysis of the microclimatic state, terrain structure and flora composition of the territory where the Artemisia schrenkiana Ledeb plant grows, described 163 plant species belonging to 116 genera in 31 families from the plant community. In the areas where the plant Artemisia schrenkiana Ledeb grows, the following five families predominate in terms of the number of species: the family of Poaceae or Gramineae, a family of Compositae Giseke, A family of Chenopodiaceae Vent, a family of Leguminosae Juss, a family of Boraginaceae Juss. Plants in the studied community were classified according to their useful properties as follows: 157 species of cattle, 38 species of medicinal plants, 5 species of poisonous plants, 10 species of essential oil plants, 3 species of rotting plants, 3 species of ornamental plants. Currently, no external factors have a strong negative impact on the plant community in the teritorry of the Urzhar district, where the medicinal plant Artemisia schrenkiana Ledeb grows. In the future, it will be possible to collect reserves of useful and medicinal plants from this region.","PeriodicalId":248021,"journal":{"name":"BULLETIN of the L.N. Gumilyov Eurasian National University. BIOSCIENCE Series","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132043524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0