M. V. Sycheva, Yuliya Peshkova, O. L. Kartashova, A. Andreeva
{"title":"REGULATION OF MICROORGANISMS SENSITIVITY TO ANTAGONISTIC ACTIVE REPRESENTATIVES OF MUTUALISTICALLY MICROFLORA BY THE ANTIMICROBIAL PEPTIDES","authors":"M. V. Sycheva, Yuliya Peshkova, O. L. Kartashova, A. Andreeva","doi":"10.36233/0372-9311-2017-6-21-25","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-6-21-25","url":null,"abstract":"Aim. Study of the effect of antimicrobial peptides (AMP) on the sensitivity of the conditional pathogenic of bacteria to the action of antagonistic active of representatives mutualistically microflora. Materials and methods. The study used peptides from platelets of chicken obtained by the method of reversed-phase high-performance liquid chromatography in stepwise and linear gradients of increasing concentrations of organic solvent. The effect of AMP on the sensitivity of microorganisms to antagonistic active substances of the dominant bacteria of the genus Lactobacillus, and Enterococcus were investigated in a joint incubation. Antagonistic activity of bacteria studied the gup method. Results. Shown an increased expression of antagonistic activity of indigenous microflora in relation to associative microflora after concubinage with a peptide fractions from platelets of chicken. Conclusion. The obtained results allow to assume the existence of new mechanisms of formation of the colonization resistance of the biotope.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"61 1","pages":"21-25"},"PeriodicalIF":0.0,"publicationDate":"2017-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84904871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Roman Gorodnichev, D. Rakitina, A. Manolov, Yu. P. Baykova, P. Scherbakov, G. B. Smirnov, E. Ilina
{"title":"FEATURES OF ESCHERICHIA COLI CLINICAL STRAINS, ISOLATED FROM THE PATIENTS WITH CROHN’S DISEASE","authors":"Roman Gorodnichev, D. Rakitina, A. Manolov, Yu. P. Baykova, P. Scherbakov, G. B. Smirnov, E. Ilina","doi":"10.36233/0372-9311-2017-6-42-49","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-6-42-49","url":null,"abstract":"Aim. To characterize pool of Crohn’s disease-associated E.coli isolated from patients with Crohn’s disease. Materials and methods. 28 clinical stains were selected. Clinical isolates, as well as control samples, were tested for their possible belonging to a certain phylogenetic group, the ability to adhere and invade on the model of a monolayer of CaCo2 epithelial cells, the ability to form biofilms and their mobility. Results. We have shown that E.coli, isolated from a different biomaterial, belonged to different phylogenetic groups and differed in their ability to form biofilms. Adhesive-invasive E.coli were found mainly in the material of biopsy of the intestinal mucosa of patients with Crohn’s disease. Conclusion. There are reasons to suppose that adhesive-invasive E.coli formed at the junction of ecological niches of the lumen and the intestinal wall.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"6 1","pages":"42-49"},"PeriodicalIF":0.0,"publicationDate":"2017-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80852384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Maletskaya, V. Dubyansky, A. Belyaeva, L. Shaposhnikova, Aga Ds, N. Ermolova, N. V. Tsapko, T. Taran, E. Kotenev, O. Belova, A. Kulichenko
{"title":"ESTIMATE OF VIRUS ZIKA SPREAD RISK IN THE REPUBLIC OF ABKHAZIA ASSOCIATING THE LOCAL POPULATION OF MOSQUITOES AEDES AEGYPTI AND AEDES ALBOPICTUS","authors":"O. Maletskaya, V. Dubyansky, A. Belyaeva, L. Shaposhnikova, Aga Ds, N. Ermolova, N. V. Tsapko, T. Taran, E. Kotenev, O. Belova, A. Kulichenko","doi":"10.36233/0372-9311-2017-6-10-15","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-6-10-15","url":null,"abstract":"","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"3 1","pages":"10-15"},"PeriodicalIF":0.0,"publicationDate":"2017-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89369235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. V. Sazanova, T. P. Shmelkova, A. L. Kravtsov, T. A. Malyukova, Yu. A. Popov
{"title":"ПРОТОЧНО-ЦИТОФЛУОРИМЕТРИЧЕСКИЙ АНАЛИЗ ЦИТОТОКСИЧНОСТИ ШТАММОВ YERSINIA PESTIS","authors":"E. V. Sazanova, T. P. Shmelkova, A. L. Kravtsov, T. A. Malyukova, Yu. A. Popov","doi":"10.36233/0372-9311-2017-6-3-9","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-6-3-9","url":null,"abstract":"Aim. Study cytotoxic effect of Yersinia pestis strains with various degree of virulence on leukocytes of whole defibrinated human blood. Materials and methods. 15 strains of Y. pestis were used in the study. DNA histograms were registered using flow cytofluorimeter. Results. Possibility of using cytotoxicity to differentiate Y. pestis into virulent and avirulent w f as experimentally proven. The data obtained was comparable with LD 5 o for mice and did not contradict molecular-genetic characteristics of the strains. Conclusion. The approach proposed allows to obtain reliable results and is perspective for evaluation of virulence for Y. pestis strains.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"13 1","pages":"3-9"},"PeriodicalIF":0.0,"publicationDate":"2017-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81904317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Вл В. Шкарин, Ольга Васильевна Ковалишена, Н. В. Саперкин, О. Н. Шпрыкова
{"title":"ОБЩАЯ ХАРАКТЕРИСТИКА И ПРОБЛЕМНЫЕ ВОПРОСЫ ПОЛИЭТИОЛОГИЧНЫХ ИНФЕКЦИЙ, ВЫЗЫВАЕМЫХ УСЛОВНО ПАТОГЕННЫМИ МИКРООРГАНИЗМАМИ","authors":"Вл В. Шкарин, Ольга Васильевна Ковалишена, Н. В. Саперкин, О. Н. Шпрыкова","doi":"10.36233/0372-9311-2017-6-114-126","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-6-114-126","url":null,"abstract":"Infections caused by opportunistic microorganisms occupy a special position. In microbial associations of opportunistic microorganisms is easier to implement its low pathogenic potential, activate properties of each other. Group polyetiologic infections caused by conditionally pathogenic microorganisms are quite diverse in terms of a nosological forms and groups. These include the so-called septic infection, which are also very diverse and clinical, epidemiological and etiological characteristics. Scientific evidence shows the urgency of the problem mixed infections, especially in surgery and indicate a lack of systematic data about the whole spectrum and the composition of microbial associations in different infections, quantification, insufficient study of the properties compared to monoagents. The problem mixed infections practically not been studied with epidemiologic standpoint, only a few studies devoted to the study of nosocomial infection of microbial associations are not developed microbiological monitoring. In this connection, you need comprehensive research mixed infections compared with monoagents to identify the characteristics and development of epidemiologic surveillance and control.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"1 1","pages":"114-126"},"PeriodicalIF":0.0,"publicationDate":"2017-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77179217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. G. Karnaukhov, N. B. Cheldyshova, A. K. Grazhdanov, A. A. Krizky, S. P. Zadnova, O. V. Kedrova, A. V. Ivanova, V. Toporkov, N. Smirnova, A. .. Boiro, S. Boumbali, M. Boiro, V. Kutyrev
{"title":"EPIDEMIC ANALYSIS ON CHOLERA IN AFRICA AND PROBLEMS OF PROFILAXIS","authors":"I. G. Karnaukhov, N. B. Cheldyshova, A. K. Grazhdanov, A. A. Krizky, S. P. Zadnova, O. V. Kedrova, A. V. Ivanova, V. Toporkov, N. Smirnova, A. .. Boiro, S. Boumbali, M. Boiro, V. Kutyrev","doi":"10.36233/0372-9311-2017-6-105-113","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-6-105-113","url":null,"abstract":"","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"1 1","pages":"105-113"},"PeriodicalIF":0.0,"publicationDate":"2017-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72736241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Khokhlova, O. Peryanova, O. Bobrova, V. K. Sergeeva, A. A. Modestov, O. G. Eremeeva, N. K. Potkina, D. N. Kapshuk, A. V. Alabusheva, Tatsuo Yamamoto
{"title":"MOLECULAR AND GENETIC FEATURES OF THE METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) - CAUSATIVE AGENTS OF PURULENT DISEASES AT CANCER PATIENTS","authors":"O. Khokhlova, O. Peryanova, O. Bobrova, V. K. Sergeeva, A. A. Modestov, O. G. Eremeeva, N. K. Potkina, D. N. Kapshuk, A. V. Alabusheva, Tatsuo Yamamoto","doi":"10.36233/0372-9311-2017-6-15-20","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-6-15-20","url":null,"abstract":"","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"15 1","pages":"15-20"},"PeriodicalIF":0.0,"publicationDate":"2017-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86315374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Н. Жданова, ' О.Б.Огарков, В. Синьков, ПЛ. Хромова, Е. А. Орлова, М. Е. Кощеев, Е. Д. Савилов, N. Zhdanova, O. Ogarkov, V. Sinkov, P. Khromova, E. Orlova, M. Koscheev, E. Savilov
{"title":"EPIDEMIOLOGICAL STUDY OF MAIN CLONES DISTRIBUTION GENOTYPE BEIJING OF MYCOBACTERIUM TUBERCULOSIS WITHIN IRKUTSK REGION","authors":"Н. Жданова, ' О.Б.Огарков, В. Синьков, ПЛ. Хромова, Е. А. Орлова, М. Е. Кощеев, Е. Д. Савилов, N. Zhdanova, O. Ogarkov, V. Sinkov, P. Khromova, E. Orlova, M. Koscheev, E. Savilov","doi":"10.36233/0372-9311-2017-6-88-94","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-6-88-94","url":null,"abstract":"To the pathogen in the Irkutsk region by comparison of genotypes of M. tuberculosis from patients of different age groups. Materials and methods. 588 epidemiologically unrelated strains of M. tuber culosis isolates from 567 ТВ patients were characterized using 24-locus MIRU-VNTR typing. 160 strains belonged to patients of different age groups. 59 strains were isolated from the «young» people with ТВ who were bom after 1990 and 101 isolates from people who were bom before 1955. Results. Two-thirds of the samples (427/567) were genotype Beijing with the dominance of two subtypes belonging to the modem epidemic clonal complexes CC1 and CC2. The high level of clustering profiles of СС 1 and CC2 genotype Beijing in «older» and «young» patients in the Irkutsk region indicates the presence and active transmission of epidemic CC1 and CC2 subtypes over the past fifty years.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"26 1","pages":"88-94"},"PeriodicalIF":0.0,"publicationDate":"2017-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83918871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Kudryashova, O. Borisova, N. Mikhailova, D. V. Lonshakov, A. V. Katlinsky
{"title":"ВЛИЯНИЕ МЕТОДОВ ИММОБИЛИЗАЦИИ ЭРИТРОПОЭТИНА НА ЧУВСТВИТЕЛЬНОСТЬ ВЫЯВЛЕНИЯ СПЕЦИФИЧЕСКИХ IgG К EПB В СЫВОРОТКАХ КРОВИ ЭКСПЕРИМЕНТАЛЬНЫЙ ЖИВОТНЫХ","authors":"A. Kudryashova, O. Borisova, N. Mikhailova, D. V. Lonshakov, A. V. Katlinsky","doi":"10.36233/0372-9311-2017-6-49-55","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-6-49-55","url":null,"abstract":"Aim. The study of the effect of the erythropoietin coating procedure on sensitivity using the same secondary detection methods to quantify anti-EPO IgG positive animal sera. Materials and methods. Sera from experimental animals - rabbits and guinea pigs - after rhEPO injection were used. The methods includes directly coated ELISA and two types of immunochemical immobilization: capturing biotinylated rhEPO on streptavidin coated microtiier plates and capturing rhEPO via a specific antibody Results. Immunochemical rhEPO immobilization results in a sensitivity from 2 to 10 of magnitude higher than direct coating of rhEPO. Conclusion. Our findings show that the method of rhEPO immobilization to microtiter plates is a critical determinant for the sensitivity of ELISA used for measuring anti-EPO antibodies. Assays in which rhEPO was captured via a specific mAb, or in which biotinylated rhEPO was captured via streptavidin, are preferred to detect serum antibodies to native structural state.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"16 5 1","pages":"49-55"},"PeriodicalIF":0.0,"publicationDate":"2017-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87994937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Alatortseva, Sidorov Av, L. Nesterenko, L. Luhverchik, M. V. Zhukina, I. I. Amiantova, A. Milovanova, D. Vorobev, Yu. I. Ammur, M. Mikhailov, K. K. Kyuregyan, V. Kichatova, I. Potemkin, O. Isaeva, E. Malinnikova, A. Karlsen, V. Blinov, Z. Nurmatov, A. Z. Nurmatov, O. Kasymov, S. Zhavoronok, Zverev Vv
{"title":"DESIGN OF HEPATITIS E VIRUS GENOTYPE 1 RECOMBINANT ORF3 PROTEIN BY CODON OPTIMIZATION METHOD","authors":"G. Alatortseva, Sidorov Av, L. Nesterenko, L. Luhverchik, M. V. Zhukina, I. I. Amiantova, A. Milovanova, D. Vorobev, Yu. I. Ammur, M. Mikhailov, K. K. Kyuregyan, V. Kichatova, I. Potemkin, O. Isaeva, E. Malinnikova, A. Karlsen, V. Blinov, Z. Nurmatov, A. Z. Nurmatov, O. Kasymov, S. Zhavoronok, Zverev Vv","doi":"10.36233/0372-9311-2017-6-63-72","DOIUrl":"https://doi.org/10.36233/0372-9311-2017-6-63-72","url":null,"abstract":"sensitivity to antagonistic active representatives of mutualistically microflora by the antimicrobial peptides","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"8 1","pages":"63-72"},"PeriodicalIF":0.0,"publicationDate":"2017-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90392532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}