Zhurnal mikrobiologii, epidemiologii, i immunobiologii最新文献

{"title":"ИДЕНТИФИКАЦИЯ ИММУНОГЕННЫХ БЕЛКОВ ШТАММОВ BACILLUS ANTHRACIS В MALDITOF MS","authors":"M. P. Chervakova, T. Sharov, L. P. Barkova, A. Barkov, D. Viktorov, A. Toporkov","doi":"10.36233/0372-9311-2018-1-52-57","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-1-52-57","url":null,"abstract":"Aim. Identification of obtained in host-simulated conditions immunogenic proteins of isogenic variants of Bacillus anthracis 575/122. Materials and methods. We used culture filtrate of isogenic variants of B. anthracis 575/122: R02 (pXOL pXO2 + ); R01 (pXO1 + pXO2‘); R00 (pXOL pX02~), obtained in host-simulated conditions. In the one-dimensional polyacrylamide gel electrophoresis and immunoblotting with hyperimmune serums immunodominant proteins, that have been identified in MALDI TOF MS. Results. Immunoblotting revealed proteins with molecular masses in range 97 - 14.1 kDa. 90 kDa protein from strain B. anthracis 575/122 R01 in MALDI TOF MS was identified as protective antigen with 85.810 kDa. Protein with molecular mass 60 kDa was identified as GMP synthase with molecular mass 57.239 kDa. In the culture filtrates of three strains two common antigen were identified: protein with molecular mass 97 kDa, identified as B. anthracis EA 1 with molecular mass 91.361 kDa protein and 45 kDa protein as enolase B. anthracis with molecular mass 46.418 kDa. Conclusion. Thus, the conditions that simulate the host can promote the production of immunodominant proteins of B. anthracis. The data about molecular-weight characteristics of protective antigen and EA 1 protein as well as some of proteases of B. anthracis are confirmed by the MALDI TOF MS. The results can be used for isolation of these proteins to improve the diagnostic and vaccine preparations.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"19 1","pages":"52-57"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84392106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EVALUATION OF THE PROGNOSTIC EPIDEMIOLOGICAL AND ECONOMIC EFFICACY OF VACCINATION AGAINST PNEUMOCOCCAL INFECTION IN MEN OF WORKING AGE WITH VARIOUS CHRONIC DISEASES","authors":"N. Briko, L. R. Batyrshina, A. Briko","doi":"10.36233/0372-9311-2018-1-17-23","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-1-17-23","url":null,"abstract":"Aim. The study of the prognosis of epidemiological and economic effects of pneumococcal vaccination in laboring males with various chronic diseases. Materials and methods. Within the predictive (Markov) model, based on the published data, assessment of 5-year horizon pneumo­ coccal vaccination of laboring males perceived effectiveness in reducing mortality, avoiding mor­ bidity and economic loss in the country had been processed. According to the official statistics in laboring are within the risk group for pneumonia. Data source for the cost of the healthcare for disease had been the compulsory health insurance system 2016 state tariffs, for the vaccination cost results of the PCV13 procurement bidding had been used. Results. Data extrapolation from national and international studies to the cohort of individuals with chronic respiratory diseases, cardiovascular diseases and diabetes mellitus vaccinated against pneumococcal infection showed a significant decrease in the risk of un­ derlying diseases complications (OP=0,58, p<0,05), hospitalizations number (OP=0,02, p<0,05) and expected mortality. The cost of vaccination in the evaluated group of patients was 25 869.5 min RUB. According to the modeling results PCV13 use will allow to statistically significantly decrease the number of relapses and hospitalizations that will permit to save up to 14 359.9 min rubles annually. Thus, in the two-year horizon, the total fiscal savings will amount to 2 850.30 million RUB and at least 61 702 of the patients' lives retained in a 5-year term with a single dose PCV13 administration. Conclusion. The study results indicate potential high epidemiological and clinical effectiveness of pneumococcal vaccination of the laboring males suffering from chronic diseases. Vaccination as a cost-effective investment in healthcare creates the opportunity of reduc­ tions in morbidity, number of exacerbations, hospitalization rate and mortality in the vaccinated cohort.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"16 1","pages":"17-23"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78311021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STATUS AND TRENDS OF THE EPIDEMIC SITUATION OF GROUP A STREPTOCOCCAL (GAS) INFECTIONS IN RUSSIA IN RECENT YEARS","authors":"N. Briko, E. Glushkova","doi":"10.36233/0372-9311-2018-1-10-16","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-1-10-16","url":null,"abstract":"Aim. To study the main epidemiological manifestations of GAS infection in Russia and to assess the scale of the problem. Materials and methods. A retrospective epidemiological analysis of official statistical reporting data (f. 2, f. 12) about the incidence and prevalence of the most sig­ nificant forms of group A streptococcal infection in 2009 — 2016 was performed. Results. There was a slight decrease in the total incidence of GAS infection in the Russian Federation. On the average, 1.8 million people fell ill every year, of which 600,000 were children aged 0 — 14. There was no reliable trend in incidence of acute rheumatic fever (ARF) in 2009 — 2016 (2.0 per 100 000 population). The risk group is adolescents. The main proportion is adults (64%). In recent years the incidence of rheumatic heart disease (RHD) increases rate in the adult group. Compared with the previous period (1996 — 2007), the situation with the incidence of diseases of the skin and subcutaneous tissue and prevalence of kidney disease improved. Conclusion. The findings indicate a high prevalence of streptococcal infection in the country. Aim. The study of the prognosis of epidemiological and economic effects of pneumococcal vaccination in laboring males with various chronic diseases. Materials and methods. Within the predictive (Markov) model, based on the published data, assessment of 5-year horizon pneumo­ coccal vaccination of laboring males perceived effectiveness in reducing mortality, avoiding mor­ bidity and economic loss in the country had been processed. According to the official statistics in","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"86 1","pages":"10-16"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84368679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BIOFILMS OF PATHOGENIC BURKHOLDERIA AND THEIR ROLE IN RESISTANCE TO ANTIBIOTICS","authors":"E. V. Shubnikova, L. Merinova, T. Senina, E. V. Korol', O. Merinova","doi":"10.36233/0372-9311-2018-1-101-111","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-1-101-111","url":null,"abstract":"Обзор содержит данные литературы, касающиеся основных вопросов образования биопленок возбудителями мелиоидоза и сапа (Burkholderia pseudomallei и Burkholderia mal­ lei). Рассмотренароль известных структурных элементов клеток буркхольдерий (жгутиков, пилей IVтипа, ЛПС), а также белков-автотранспортеров адгезинов в прикреплении бак­ терий к поверхностям, формировании микроколоний и образовании биопленки. Представлены материалы исследования генетических регуляторных механизмов (QS-систем, RpoE-сигма фактора, c-di-GMP, двухкомпонентной системы трансдукции сигналов TCS) и данные о дифференциально экспрессирующихся генах, имеющих отношение к форми­ рованию биопленки В. pseudomallei. Приведены результаты изучения роли биопленок в вирулентности и резистентности к антибиотикам патогенных буркхольдерий и их значения в развитии хронических форм и рецидивирующего течения мелиоидоза и сапа.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"12 1","pages":"101-111"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78088719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ИСПОЛЬЗОВАНИЕ МАСС-СПЕКТРОМЕТРИЧЕСКОГО АНАЛИЗА ДЛЯ ДЕТЕКЦИИ БАКТЕРИАЛЬНЫХ ТОКСИНОВ","authors":"М. В. Полеева, Ольга Чемисова","doi":"10.36233/0372-9311-2018-1-93-101","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-1-93-101","url":null,"abstract":"Toxins - molecular weight compounds produced by microorganisms, animals, plants and possessing antigene properties. Recently due to the perceived threat of terrorist actions identification of a number of bacterial toxins is especially important. A new approach in the identification of toxins associated with the development of mass spectrometry and can be successfully used for analysis of most environmental toxins. The method of MALDI-MS allows the detection of toxins such as Shiga-toxin Escherichia coli, delta-toxin of Staphylococcus aureus, Bacillus cereus particular, botulinum neurotoxin, cholera toxin. Analytical and diagnostic characteristics of the method, the simplicity and speed studies indicate the long term implementation of a method in the practice of laboratory diagnostics in determining toxinproducing of the studied microorganisms.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"38 1","pages":"93-101"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85923354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BACTERICIDAL EFFECT OF HUMAN SERUM ON BORRELIA MIYAMOTOI, CAUSATIVE AGENT OF IXODES TICK-BORNE BORRELIOSIS (ITBB-BM)","authors":"A. Platonov, J. Koetsveld, N. Kolyasnikova, O. Stukolova, A. Dolgova, M. Toporkova, D. S. Sarksyan","doi":"10.36233/0372-9311-2018-2-30-38","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-2-30-38","url":null,"abstract":"Aim. Our aim was to study the bactericidal effect of human serum on Borrelia miyamotoi in vitro. Materials and methods. B. miyamotoi spirochetes (strains HT31 and LB-2001) were incu­ bated in non-immune serum of healthy donors (SHD) and in heat inactivated complement-de­ pleted SHD, as well as in serum samples of the patients recovered from ITBB-BM. The viability, that is motility, of borrelia after incubation was investigated by dark-field microscopy. The levels of serum antibody to Rmiyamofoi-specificproteins (GlpQ enzyme and four variable majorproteins Vlpl5/16, Vlpl8, Vspl, and Vlp5) were measured by specially designed plane protein microarray. Results. Borrelia Aim. Study of the effect of exopolysaccharides (EPS) of lactic acid cocci on cytokine activity of macrophages of mice with phagocytosis in vitro Staphylococcus aureus 209-P. Materials and methods. The EPS of Streptococcus thermophilus and Lactococcus lactis B-1662 was used in the work. At 1,3, 5 and 7, AMP and PMP were isolated and the phagocytosis process was modeled in vitro. After 30 minutes, 1, 6 and 24 hours, the content of pro-inflammatory cytokines IL-1 a and TNF-a was determined. Results. EPSs had an ambiguous effect on the production of cytokines. The greatest effect on the synthesis was provided by EPS of S. thermophilus. Conclusion. The results of the study allow us to talk about the possibility of using EPS of S. thermophilus as a preventive immunomodulator for correction of the cytokine status of animals.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"1 1","pages":"30-38"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83715715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SEVERE CASES OF SEASONAL INFLUENZA IN RUSSIA IN 2015 - 2016 AND 2016 - 2017","authors":"S. Svyatchenko, Durymanov Ag, I. Susloparov, N. Kolosova, N. I. Goncharova, O. Petrova, A. Epanchintseva, A. V. Maksyutova, K. Kondik, O. K. Slaboda, E. Ivanova, V. Mikheev, Ryzhikov Ab, T. Ilicheva","doi":"10.36233/0372-9311-2018-1-32-39","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-1-32-39","url":null,"abstract":"","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"30 1","pages":"32-39"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76558925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MOLECULAR-GENETIC CHARACTERISTICS OF SHIGELLA SONNEI-2013 STRAIN ISOLATED DURING THE OUTBREAK IN DYSENTERY IN THE REPUBLIC ABKHAZIA IN 2013","authors":"O. Vasileva, A. Volynkina, I. Kuznetsova, S. Pisarenko, A. Kulichenko","doi":"10.36233/0372-9311-2018-1-72-76","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-1-72-76","url":null,"abstract":"","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"43 1","pages":"72-76"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79765621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EFFECT OF EXOPOLISACCHARIDES OF LACTIC ACID BACTERIA ON THE SYNTHESIS OF PROINFLAMMATORY CYTOKINES BY MACROPHAGIC MICE IN PHAGOCYTOSIS OF STAPHYLOCOCCUS AUREUS","authors":"G. T. Uryadova, E. Gorelnikova, N. Fokina, A. S. Dolmashkina, L. Karpunina","doi":"10.36233/0372-9311-2018-1-67-71","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-1-67-71","url":null,"abstract":"Aim. Study of the effect of exopolysaccharides (EPS) of lactic acid cocci on cytokine activity of macrophages of mice with phagocytosis in vitro Staphylococcus aureus 209-P. Materials and methods. The EPS of Streptococcus thermophilus and Lactococcus lactis B-1662 was used in the work. At 1,3, 5 and 7, AMP and PMP were isolated and the phagocytosis process was modeled in vitro. After 30 minutes, 1, 6 and 24 hours, the content of pro-inflammatory cytokines IL-1 a and TNF-a was determined. Results. EPSs had an ambiguous effect on the production of cytokines. The greatest effect on the synthesis was provided by EPS of S. thermophilus. Conclusion. The results of the study allow us to talk about the possibility of using EPS of S. thermophilus as a preventive immunomodulator for correction of the cytokine status of animals. Aim. Study of molecular-genetic properties of Shigella sonnei- 2013 strain isolated during the outbreak in dysentery in the republic Abkhazia in 2013. Materials and methods. Genetic typing of the tested strains using multilocus sequence typing (MLST). Analyzed of nucleotide sequence fragments 7 of conservative «housekeeping» genes adk, fumC, icd, mdh, purA, recA, gyrB. Sequenced of DNA fragments compared with reference sequences from database of Escherichia coli MLST. Phylogenetic analysis was performed using UPGMA method and computer program START 2. Whole-genome sequencing performed on a genetic analyzer Ion Torrent Personal Genome Machine (PGM™) using fragment libraries (shot-gun). Aligning reads have been carried out with the program GS Reference Mapper. Results. Defined sequence — type of the studied strain — ST-152, one of the most common genotypes for S. sonnei. Demonstrated the high degree of similarity obtained contig to the sequences of the chromosome and plasmids А, В, С и E strains S. sonnei 53G and S. sonnei Ss046. Identified contigs with a high percentage similarity to the se­ quence of virulence plasmid p026-Vir of E. coli 026:H11","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"29 1","pages":"67-71"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81170411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ФУНКЦИОНАЛЬНЫЕ ГРУППЫ БИФИДОФЛОРЫ КИШЕЧНОЙ МИКРОБИОТЫ В АССОЦИАТИВНОМ СИМБИОЗЕ ЧЕЛОВЕКА","authors":"О. В. Бухарин, Е. В. Иванова, Н. Б. Перунова, Игорь Анатольевич Никифоров","doi":"10.36233/0372-9311-2018-1-3-9","DOIUrl":"https://doi.org/10.36233/0372-9311-2018-1-3-9","url":null,"abstract":"Aim. Aim of the research is the identification of functional groups of human gut bifidoflora based on analysis of the spectrum of metabolites features, proteome, bioprofile, immunoregulatory properties and the ability to differentiate «self/non-self» among the associative microbiota. Materials and methods. The materials are 260 strains ofbifidobacteria isolated from 122 intestinal microsymbiocenoses. Experimental studies were carried out using bacteriological, chromatographic and immunological methods. Statistical processing of material is carried out by means of the package Statistica 10.0 using of k-cluster analysis and discriminant method. Results. As a result ofthe work, 3 clusters containing strains of various types of bifidobacteria were identified. The first cluster was represented by B. bifidum and was characterized by the antipeptide activity of the strains with respect to FNO-a and INF-y, IL-10. In the second cluster of the B. longum culture predominated, where the parameters of the backbone factor of microsymbiocenosis, the ability to microbial recognition, antagonistic activity and production of acetic acid were significant. In the third cluster the species composition of bifidobacteria was diverse and products of butyric, caproic acids and their isoforms were the informative tests. Conclusion. The key function of bifido-flora in the regulation of the homeostasis of the intestinal biotope is realized by the formation of functional clusters, among which the first group participates in the formation of the cytokine balance, the second group is responsible for the discrimination of associative microbiota and direct protection of the biotope from pathogens, and the third is necessary to maintain the barrier metabolic function of enterocytes in the human large intestine.","PeriodicalId":24020,"journal":{"name":"Zhurnal mikrobiologii, epidemiologii, i immunobiologii","volume":"1 1","pages":"3-9"},"PeriodicalIF":0.0,"publicationDate":"2018-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90966245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}