Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology最新文献_第8页

Isolation and identification of influenza viruses from clinical materials in 1977-1993 at Veterans General Hospital-Taipei. 台北荣民总医院1977 ~ 1993年临床资料中流感病毒的分离与鉴定。

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology Pub Date : 1995-05-01

W T Liu, H Y Wei, S T Hu, C H Tsai, S R Chern, H C Wang

引用次数: 0

A method of stimulating an immune response with a hapten immobilized on a nitrocellulose membrane. 一种用固定在硝化纤维素膜上的半抗原刺激免疫反应的方法。

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology Pub Date : 1995-02-01

J J Liu, C C Wang, Y L Hwang, S Yue, T H Chang

引用次数: 0

[Studies on the susceptibility of two northern Taiwan strains of Aedes albopictus mosquitoes to Japanese encephalitis virus]. 台湾北部2株白纹伊蚊对日本脑炎病毒的敏感性研究。

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology Pub Date : 1995-02-01

M H Weng, J C Lien, Y S Show

引用次数: 0

[Coagulase type and antimicrobial susceptibility of Staphylococcus aureus isolated from various areas in Taiwan]. [台湾不同地区分离的金黄色葡萄球菌凝固酶类型及药敏分析]。

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology Pub Date : 1995-02-01

S E Hsieh, J L Liu

{"title":"[Coagulase type and antimicrobial susceptibility of Staphylococcus aureus isolated from various areas in Taiwan].","authors":"S E Hsieh, J L Liu","doi":"","DOIUrl":"","url":null,"abstract":"A total of 129 strains of Staphylococcus aureus, isolated from clinical specimens in Taiwan between February 1992 and December 1993, were subjected to coagulase typing and susceptibility testing to 21 kinds of antimicrobial agents using Pasco MIC Gram-positive panels. In the determination of minimum inhibition concentration (MIC), there were 94 strains (72.9%) resistant to penicillin and ampicillin, 54 strains (41.9%) resistant to tetracycline and erythromycin, and 21 strains (16.3%) resistant to oxacillin (oxacillin-resistant S. aureus; ORSA), but none of them was resistant to vancomycin or nitrofurantoin. As the susceptibility of the isolates from four different geographic districts was compared, no statistical difference was found except that the resistance rate to penicillin and ampicillin was higher in southern Taiwan, and resistance rate to rifampin and gentamicin was higher in central Taiwan. The ORSA strains were all resistant to penicillin, ampicillin, tetracycline; 95.2% of the strains were resistant to gentamicin, tobramycin and erythromycin. The resistance rates to drugs tested for ORSA strains were statistically higher than those for OSSA strains except vancomycin, nitrofurantoin, trimethoprim/sulfamethoxazole and ampicillin/sulbactam. In the coagulase typing of 127 strains, Type IV, III and VII were most frequently encountered. Among the coagulase types, Type IV was mostly encountered in the North, the South and the East of Taiwan; Type III was mostly encountered in central Taiwan. Among the ORSA strains, coagulase Type III was most predominant (85%). In conclusion, analysis of an antibiogram is easy to perform and the results can provide clinicians not only with correct guides for patient treatment but also with a useful tool for epidemiological studies. However, if antibiogram and coagulase typing are carried out simultaneously, results will be more reliable in epidemiological studies, including nosocomial infection survey.","PeriodicalId":24009,"journal":{"name":"Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1995-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20687471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differentiation of Vibrio vulnificus strains by an arbitrarily primed polymerase chain reaction. 用任意引物聚合酶链反应分化创伤弧菌菌株。

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology Pub Date : 1995-02-01

J J Wu, L I Hor, S L Shiau

引用次数: 0

Purification of glucosyltransferases (GtfB/C and GtfD) from mutant strains of Streptococcus mutans. 从变形链球菌突变株中纯化葡萄糖基转移酶(GtfB/C和GtfD)。

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology Pub Date : 1995-02-01

J S Chia, C C Hsieh, C S Yang, J Y Chen

{"title":"Purification of glucosyltransferases (GtfB/C and GtfD) from mutant strains of Streptococcus mutans.","authors":"J S Chia, C C Hsieh, C S Yang, J Y Chen","doi":"","DOIUrl":"","url":null,"abstract":"Streptococcus mutants constitutively expresses three glucosyltransferases (GTFs), i.e., GtfB, GtfC, and GtfD, which synthesize glucan polymers from sucrose. Two genetically constructed mutants of S. mutans which stably expressed either the cell-associated or the extracellular GTFs were selected for purification and characterization of these enzymes. The cell-associated GtfB and GtfC from strain GS-5DD lacking the gtfD gene expression were extracted by urea, renatured by dialysis in sodium phosphate buffer and then separated from the other wall-associated components by column chromatography. The extracellular GtfD was purified from the culture supernatant of strain NHS1 lacking gtfB and gtfC gene expression. The molecular weights of the purified GTFs was similar (150-160 kDa), as determined by SDS-polyacrylamide gel electrophoresis. The GtfB/C preparation synthesized primarily water-insoluble glucan in a primer independent manner. However, the presence of the dextran enhanced the enzymatic activities of the GtfB/C. GtfD synthesized water-soluble glucan exclusively in a primer dependent manner. Purified GtfD had a pH optimum of 5.5, and a K(m) value of 4.35 mM for sucrose. These results indicated that the mutated strains served as an efficient and specific host to obtain native GTFs.","PeriodicalId":24009,"journal":{"name":"Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1995-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20687466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphogenesis, biomass and oxytetracycline production of Streptomyces rimosus in submerged cultivation. 水培链霉菌的形态发生、生物量和土霉素产量。

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology Pub Date : 1995-02-01

J Y Wang, S S Yang

引用次数: 0

Immunity to diphtheria in children three-to-six year of age in four counties of Taiwan. 台湾四县三至六岁儿童对白喉的免疫。

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology Pub Date : 1995-02-01

H F Lee, M C Wang, L R Tseng, C F Lu, H M Hsu, C B Horng

{"title":"Immunity to diphtheria in children three-to-six year of age in four counties of Taiwan.","authors":"H F Lee, M C Wang, L R Tseng, C F Lu, H M Hsu, C B Horng","doi":"","DOIUrl":"","url":null,"abstract":"An immunization program against diphtheria has been implemented in Taiwan since 1955, using combined diphtheria, pertussis and tetanus (DPT) vaccine. Diphtheria immunoglobulin (DIG) level was assessed in serum samples obtained from 1138 children, aged 3-6 years from north, south, east and central part of Taiwan by the VERO cell neutralization method. Specimens were collected by simple random sampling of residents from Hsinchu, Taichung, Pingtung and Hwalien counties, including both aborigines and non-aborigines. The former lived in one or two villages in each county, and the latter lived in a single village next to the former. Ninety-five percent (1086/1138) had a DIG titre > or = 0.01 IU/ml. There was no significant difference by sex, or by residential area. Seventy-nine percent (901/1138) of the children had completed the primary immunization schedule (at the age of 2, 4, 6 and 18 months), and the prevalence of DIG titre > or = 0.1 IU/ml considered to be long-term protective was as follows: 74.6% for 3-year group; 74.5% for 4-year group; 67.9% for 5-year group; 84.7% for 6-year group (including 52.2% who had had a booster shot at early primary school). These findings show that the diphtheria vaccination program provides good immunity in childhood.","PeriodicalId":24009,"journal":{"name":"Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1995-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20687469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphogenesis, biomass and oxytetracycline production of Streptomyces rimosus in submerged cultivation. 水培链霉菌的形态发生、生物量和土霉素产量。

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology Pub Date : 1995-02-01 DOI: 10.6946/ASRNL.199609.0068

J. Y. Wang, S. S. Yang

引用次数: 6

[Survey of disease of cultural shrimp in Taiwan]. [台湾养殖虾病调查]。

Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology Pub Date : 1995-02-01

W Y Liu, G H Kou, S N Chen

{"title":"[Survey of disease of cultural shrimp in Taiwan].","authors":"W Y Liu, G H Kou, S N Chen","doi":"","DOIUrl":"","url":null,"abstract":"The large number of hemocytes infiltrated several abnormal tissues of kuruma shrimp (Penaeus japonicus), including musculature, hepatopancreas, lymphoid organ, gill filament and sponge tissue. In addition, there were many denatured hemocytes existing inside acidophilic particles and forming granules. Futhermore, in hepatopancreas of kuruma shrimp, a white spot baculovirus (WSBV; 40-50 x 50-300 nm) was discovered in UH (undifferential haemocyte). The epithelium cells, which including stomach cuticle and underlying epidermis of exoskeletal cuticle, could also be infected by WSBV in another main cultural species--grass shrimp (P. monodon). During a period of high water temperature, with pond shrimp in normal condition, the CFU/ml of water bacteria rose from 10(5) to 10(7), but this number had decreased to 10(5) CFU/ml by the time moribund shrimp began to appear. Coincidentally, the total bacterial number isolated from hepatopancreas and musculature of moribound shrimp was over 10(5) (CFU/g) and 10(3)-10(5), respectively. The fauna of bacteria was taken over by the active metabolitic species which were represented by Vibrio species causing the pond shrimp to undergo either behavioral changes, such as swimming on the water surface, or histological changes, such as having whitish muscle color, hemocyte infiltration and granuloma formation etc. Pathogenetic species of Vibrio including V. parahaemolyticus, V. alginoly ticus V. anguillarum, V. fischery and V. damsela were isolated from those tissues of moribund shrimp. The main pathogens, isolated from musculature and hepatopancreas, were V. parahaemolyticus and V. alginolyticus. On the other hand there, was no bacterium could be isolated from the musculature of healthy shrimp and only a single species of Gram (+) coccus--Micrococcus--was isolated from the tissue of hepatopancreas.","PeriodicalId":24009,"journal":{"name":"Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1995-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20687472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0