一种用固定在硝化纤维素膜上的半抗原刺激免疫反应的方法。

J J Liu, C C Wang, Y L Hwang, S Yue, T H Chang
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引用次数: 0

摘要

半抗原是指一种小摩尔质量(通常小于1000道尔顿)的化合物,它可以与抗体结合,但自身不能启动免疫反应,除非它与较大摩尔质量的蛋白质载体结合。提出了一种制备半抗原产生抗半抗原免疫而无需与载体共价偶联的新方法。将水溶性和不溶性半抗原包覆在硝化纤维素膜上,有效地将半抗原呈递到系统中,并通过体外和体内免疫产生特异性的抗半抗原B淋巴细胞和抗体。该方法具有替代传统半抗原载体偶联产生抗半抗原免疫的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A method of stimulating an immune response with a hapten immobilized on a nitrocellulose membrane.

Hapten refers to a chemical compound of small molar mass (typically less than 1000 daltons) that can bind with an antibody, but cannot initiate an immune response by itself unless it is conjugated to a protein carrier of larger molar mass. A novel method to prepare a hapten to generate anti-hapten immunity without covalent conjugation to a carrier was developed. Coating both water-soluble and -insoluble haptens onto a nitrocellulose membrane effectively presented haptens to the system and caused the generation of specific anti-hapten B lymphocytes and antibodies by immunization both in vitro and in vivo. This method has a potential to substitute for conventional hapten carrier conjugation to generate anti-hapten immunity.

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