水培链霉菌的形态发生、生物量和土霉素产量。

J Y Wang, S S Yang
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引用次数: 0

摘要

分光光度法、高效液相色谱法和荧光素-荧光素酶法检测到的三磷酸腺苷(ATP)最低浓度分别为1.0微米、3.3微米和100纳米。在潜水培养中,大多数链霉菌TM-55在65 h时呈菌丝片段形式,在166 h时变成短杆菌丝,在504 h时裂解。ATP含量在24 h时达到最大值,在培养过程中逐渐降低。随着孵育时间的增加,土霉素效价逐渐升高,在孵育166 h时达到最大效价178.9微克/ml,随后逐渐降低。在链霉菌潜水培养中,形态发生在土环素生产中起着重要作用;短杆菌丝体土霉素产量高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Morphogenesis, biomass and oxytetracycline production of Streptomyces rimosus in submerged cultivation.

The minimal concentration of adenosine triphosphate (ATP) which could be detected with spectrophotometry, HPLC and luciferin-luciferase methods was 1.0 microM, 3.3 microM and 100 nM, respectively. In submerged cultivation, most Streptomyces rimosus TM-55 was in hyphae fragment form at 65 h, became short-rod mycelia at 166 h, and lysed at 504 h incubation. The ATP content had maximal value at 24 h, then gradually decreased during cultivation. The oxytetracycline potency increased as incubation occurred, had maximal potency 178.9 micrograms/ml at 166 h, and then gradually decreased. Morphogenesis was very important in oxytetracycline production in submerged cultivation of Streptomyces; short-rod mycelia had high oxytetracycline production.

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