用任意引物聚合酶链反应分化创伤弧菌菌株。

J J Wu, L I Hor, S L Shiau
{"title":"用任意引物聚合酶链反应分化创伤弧菌菌株。","authors":"J J Wu,&nbsp;L I Hor,&nbsp;S L Shiau","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>A synthetic 17 mer oligonucleotide (5'-GTTGGGTAACGCCAGGG-3') was used as a primer for the arbitrarily primed polymerase chain reaction (AP-PCR) to differentiate various strains of Vibrio vulnificus. A total of 37 genomic DNAs that were extracted from the clinical and environmental strains were successfully differentiated. Among them, 32 profiles of the 37 strains were characterized. None of the environmental and clinical strains had the same amplification profile, suggesting the highly heterogeneous population existed in the strains of V. vulnificus. The size of the amplified sequences ranged from 0.3 to 2.0 Kb and the DNAs were separated to 12 to 20 bands by the 1.2% agarose gel. The clinical isolates from two independent episodes of V. vulnificus infections in a patient were shown to have the same profile, indicating that the second episode was due to recurrence rather than reinfection. The profiles of amplification were reproducible with different preparations of genomic DNA. Arbitrarily primed polymerase chain reaction can therefore be a useful tool for epidemiological study of V. vulnificus infection.</p>","PeriodicalId":24009,"journal":{"name":"Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1995-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Differentiation of Vibrio vulnificus strains by an arbitrarily primed polymerase chain reaction.\",\"authors\":\"J J Wu,&nbsp;L I Hor,&nbsp;S L Shiau\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A synthetic 17 mer oligonucleotide (5'-GTTGGGTAACGCCAGGG-3') was used as a primer for the arbitrarily primed polymerase chain reaction (AP-PCR) to differentiate various strains of Vibrio vulnificus. A total of 37 genomic DNAs that were extracted from the clinical and environmental strains were successfully differentiated. Among them, 32 profiles of the 37 strains were characterized. None of the environmental and clinical strains had the same amplification profile, suggesting the highly heterogeneous population existed in the strains of V. vulnificus. The size of the amplified sequences ranged from 0.3 to 2.0 Kb and the DNAs were separated to 12 to 20 bands by the 1.2% agarose gel. The clinical isolates from two independent episodes of V. vulnificus infections in a patient were shown to have the same profile, indicating that the second episode was due to recurrence rather than reinfection. The profiles of amplification were reproducible with different preparations of genomic DNA. Arbitrarily primed polymerase chain reaction can therefore be a useful tool for epidemiological study of V. vulnificus infection.</p>\",\"PeriodicalId\":24009,\"journal\":{\"name\":\"Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1995-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zhonghua Minguo wei sheng wu ji mian yi xue za zhi = Chinese journal of microbiology and immunology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

采用人工合成的17聚寡核苷酸(5′-GTTGGGTAACGCCAGGG-3′)作为引物,采用任意引物聚合酶链反应(AP-PCR)对不同创伤弧菌进行区分。从临床菌株和环境菌株中提取的37个基因组dna成功分化。其中,对37株菌株的32个基因型进行了鉴定。环境菌株和临床菌株均没有相同的扩增谱,表明创伤弧菌存在高度异质群体。扩增序列大小在0.3 ~ 2.0 Kb之间,经1.2%琼脂糖凝胶分离出12 ~ 20条条带。一名患者两次独立的创伤弧菌感染的临床分离株显示出相同的特征,表明第二次发作是由于复发而不是再感染。扩增图谱在不同的基因组DNA制备条件下均可重复。因此,任意引物聚合酶链反应可作为创伤弧菌感染流行病学研究的有效工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Differentiation of Vibrio vulnificus strains by an arbitrarily primed polymerase chain reaction.

A synthetic 17 mer oligonucleotide (5'-GTTGGGTAACGCCAGGG-3') was used as a primer for the arbitrarily primed polymerase chain reaction (AP-PCR) to differentiate various strains of Vibrio vulnificus. A total of 37 genomic DNAs that were extracted from the clinical and environmental strains were successfully differentiated. Among them, 32 profiles of the 37 strains were characterized. None of the environmental and clinical strains had the same amplification profile, suggesting the highly heterogeneous population existed in the strains of V. vulnificus. The size of the amplified sequences ranged from 0.3 to 2.0 Kb and the DNAs were separated to 12 to 20 bands by the 1.2% agarose gel. The clinical isolates from two independent episodes of V. vulnificus infections in a patient were shown to have the same profile, indicating that the second episode was due to recurrence rather than reinfection. The profiles of amplification were reproducible with different preparations of genomic DNA. Arbitrarily primed polymerase chain reaction can therefore be a useful tool for epidemiological study of V. vulnificus infection.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信