Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology最新文献

{"title":"[Regulative effect of active components of Cistanche deserticola on intestinal dysbacteriosis induced by antibiotics in mice].","authors":"Tian-Yu Han,&nbsp;Dong Yang,&nbsp;Shu-Qing Zhou,&nbsp;Ya-Mei Qiao,&nbsp;Jing Yin,&nbsp;Min Jin,&nbsp;Jun-Wen Li","doi":"10.12047/j.cjap.6381.2022.139","DOIUrl":"https://doi.org/10.12047/j.cjap.6381.2022.139","url":null,"abstract":"<p><p><b>Objective:</b> To study the effects of Cistanche deserticola and its active components Cistanche deserticola polysaccharide and Echinacoside on intestinal flora of antibiotic-associated diarrhea (AAD) mice. <b>Methods:</b> Forty-eight Balb/c mice were randomly divided into control (Con) group, AAD Group, inulin (Inu) group, Cistanche deserticola (RCR) group, Cistanche deserticola polysaccharide (RCRDT) group and Echinacoside (Ech) group with 8 mice in each group. The diarrhea model of mice was induced by intragastric administration of lincomycin hydrochloride(3 g/kg) for 7 days, and then treated by intragastric administration of INU(5 g/kg), RCR(5 g/kg), RCRDT(200 mg/kg) and ECH (60 mg/kg),0.2 ml once a day for 7 days, Con group and AAD group were given the same volume of normal saline. By observing general signs of mice, colon HE staining, 16S rDNA high-throughput sequencing analysis, the effects of Cistanche deserticola, Cistanche deserticola polysaccharide and Echinacea glycoside on the imbalance of intestinal flora induced by antibiotics in mice were evaluated. <b>Results:</b> Compared with Con group, AAD group mice lost weight, presented obvious diarrhea symptoms, inflammatory changes in colon tissue and decreased intestinal flora diversity (<i>P</i>＜0.05) indicating the success of the model. Compared with AAD group, the weight and diarrhea of INU, RCR, RCRDT and ECH groups were significantly improved, and the colon pathology of ECH group was restored to normal level. Compared with AAD group, RCR group, RCRDT group and ECH group had significantly decreased intestinal <i>Firmicutes,</i> increased <i>Blautia</i> and <i>Lachnoclostridium,</i> and decreased <i>Clostridium_sensu_stricto_1</i> (<i>P</i>＜0.05) . In ECH group, the abundance and diversity of intestinal microflora were returned to normal level, and the structure of intestinal microflora was well adjusted, the contents of <i>Bacteroides,</i> <i>Flavonifractor,</i> <i>Agathobacter,</i> <i>Lachnoclostridium</i> and <i>Prevotella-9</i> were increased (<i>P</i>＜0.01). <b>Conclusion:</b> Both Cistanche deserticola and its active components cistanche deserticola polysaccharide and echinacoside can regulate the intestinal flora imbalance caused by antibiotics and improve the symptoms of AAD, especially echinacoside.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"766-770"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9618059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of exhaustive exercise on coagulation state in rats].","authors":"Yan-Shi Xia,&nbsp;Hong Gao,&nbsp;Dan-Wei Zhao","doi":"10.12047/j.cjap.6372.2022.130","DOIUrl":"https://doi.org/10.12047/j.cjap.6372.2022.130","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the effects of one-time exhaustive exercise on coagulation state in rats and its mechanism. <b>Methods:</b> Forty-eight SD rats were randomly divided into control group and exhaustive exercise group, 24 rats in each group. Rats in exhaustive exercise group were trained with treadmill training for 25～50 min at a time on non-slope treadmill and the initial speed of 5 m/min was uniformly accelerated to 25 m/min until the rats exhausted. Thrombelastography (TEG) was used to monitor the coagulation function of rats after training. The ligation model of inferior vena cava (IVC) was established to evaluate thrombosis. The phosphatidylserine (PS) exposure and Ca²⁺ concentration were detected by flow cytometry. The production of FXa and thrombin was detected by microplate reader. The clotting time was measured by using coagulometer. <b>Results:</b> Compared with the control group, the blood of rats in the exhaustive exercise group exhibited hypercoagulable state. The probability of thrombus formation, weight, length and ratio in the exhaustive exercise group were significantly higher than those in the control group (<i>P</i>＜0.01). The levels of PS exposure and intracellular Ca²⁺ concentration of red blood cells (RBCs) and platelets in the exhaustive exercise group were increased significantly (<i>P</i>＜0.01). The blood clotting time of RBCs and platelets was shortened (<i>P</i>＜0.01), and the production of FXa and thrombin was increased significantly (<i>P</i>＜0.01) in the exhausted exercise group, and both were inhibited by lactadherin (Lact, <i>P</i>＜0.01). <b>Conclusion:</b> The blood of exhaustive exercise rats is in a hypercoagulable state and the risk of thrombosis is increased. The increased PS exposure of RBCs and platelets caused by exhaustive exercise may be an important mechanism of thrombosis.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"714-718"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9618056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of knockdown ACC1 on glioma U251 cell migration and its mechanisms].","authors":"Lin Zhang,&nbsp;He Qian,&nbsp;Bao-Sheng Zhao,&nbsp;Man-Qi Gao,&nbsp;Yu-Zhen Liu","doi":"10.12047/j.cjap.6357.2022.136","DOIUrl":"https://doi.org/10.12047/j.cjap.6357.2022.136","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the effects of ACC1 knockdown on human glioma U251 cell migration and its molecular mechanisms. <b>Methods:</b> Human glioma U251 cell line was used. The experiment was carried out in three steps. Experiment 1: knockdown of ACC1 in U251 cells (shACC1) and its control (NC) U251 cells were established by transfection of shACC1 lentivirus and negative control virus. The cell migration was detected by Transwell migration assay and scratch test. Western blot (WB) was performed to detect the levels of ACC1, Vimentin, Fibronectin, N-cadherin, E-cadherin and Slug proteins. Experiment 2: RT-qPCR and WB were performed to verify the RNA-seq result, upregulation effect of ACC1 knockdown on PAI-1 in U251 cells. The cells then were treated with PAI-1 inhibitor PAI-039, and the cell migration was detected by Transwell migration assay and scratch assay. The protein levels of ACC1, PAI-1, Vimentin, Fibronectin, N-cadherin, E-cadherin and Slug were examined by WB. Experiment 3: the molecular mechanisms of knocking down ACC1 to increase PAI-1 were explored. The cells were treated with acetyltransferase inhibitor C646, and cell migration was examined by Transwell migration assay and scratch assay. WB was conducted to test the levels of ACC1, H3K9ac, PAI-1, Vimentin, Fibronectin, N-cadherin, E-cadherin and Slug proteins. Each experiment was repeated three times. <b>Results:</b> Experiment 1: lentivirus transfection was performed on glioma U251 cells. Compared with NC group, the expression level of ACC1 in shACC1 group was decreased significantly, indicating that lentivirus transfection was successful (<i>P</i>＜0.01), and the number of migrated cells in shACC1 group was increased significantly (<i>P</i>＜0.01). Migration-related proteins Vimentin, Fibronectin, N-cadherin and Slug were up-regulated, while E-cadherin was down-regulated (<i>P</i>＜0.01). Experiment 2: Compared with NC group, PAI-1 mRNA level in shACC1 group was up-regulated. Compared with control group, cell migration in shACC1+PAI-039 group was decreased (<i>P</i>＜0.01), and migration-related proteins Vimentin, Fibronectin, N-cadherin, and Slug were up-regulated. E-cadherin expression was down-regulated (<i>P</i>＜0.01). Experiment 3: Compared with NC group, the concentration of acetyl-coA and the expression level of H3K9ac in shACC1 group were increased significantly (<i>P</i>＜0.01); After further treatment with histone acetyl transferase inhibitor C646, PAI-1 mRNA level was decreased, cell migration number and H3K9ac expression level were decreased in shACC1+C646 group compared with control group (<i>P</i>＜0.01). Migration-related proteins Vimentin, Fibronectin, N-cadherin and Slug were up-regulated, while E-cadherin was down-regulated (<i>P</i>＜0.01). <b>Conclusion:</b> Knockdown of ACC1 promotes the migration of human glioma U251 cells by increasing histone acetylation which elevates the level of PAI-1.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"745-753"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9618058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of nanopolystyrene nanoplastic exposure on the development and neurotoxicity of fetal rats during gestation].","authors":"Ya-Ping Zhang,&nbsp;Lei Tian,&nbsp;Xiao-Qian Xie,&nbsp;Ya-Ting Wang,&nbsp;Peng Lyu,&nbsp;Zhu-Ge Xi","doi":"10.12047/j.cjap.6379.2022.138","DOIUrl":"https://doi.org/10.12047/j.cjap.6379.2022.138","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the effects of polystyrene nanoplastics (PS-NPs) exposure during gestation on the growth and neurotoxicity of fetal rats. <b>Methods:</b> Twenty-seven SD pregnant rats were randomly divided into 9 groups with three rats in each group. The experimental group of PS-NPs was given 0.5, 2.5, 10 and 50 mg/kg of PS-NPs suspension with different particle sizes (25 and 50 nm) by gavage, wihe the control group was given ultrapure water by gavage. The time of gavage is from the 1st to the 18th day of pregnancy. The morphological changes of the placenta were observed; compare the number of male and female fetuses, live/dead/absorbed fetuses, body weight, body length, placental weight, and organ coefficients of kidney, liver, brain and intestine of fetal rats; the prefrontal cortex, hippocampus and striatum of the fetal rats were taken to measure related biochemical indicators. <b>Results:</b> Compared with the control group, the placenta of the PS-NPs exposed group was found to have structural damage, which increased in a dose-dependent manner. The area ratio of trophoblast was significantly increased (<i>P</i>＜0.05), and the area ratio of labyrinth was significantly decreased (<i>P</i>＜0.05); In the prefrontal cortex, hippocampus and striatum of fetal rats, the levels of IL-1β, IL -6 and TNF-α were significantly increased in the 10 and 50 mg/kg PS-NPs exposed group (<i>P</i>＜0.05), and more significantly elevated in the 25 nm group than those in the 50 nm group at 10 mg/kg exposure (<i>P</i>＜0.05) the CAT activity was significantly decreased in 2.5, 10 and 50 mg/kg PS-NPs exposure groups (<i>P</i>＜0.05), while the SOD and GSH-Px activities were significantly decreased in 25 nm exposure groups and 2.5, 10 and 50 mg/kg 50 nm PS-NPs exposure groups (<i>P</i>＜0.05), the MDA content was significantly increased in 10, 50 mg/kg 25 nm PS-NPs exposure groups and 50 mg/kg 50 nm PS-NPs exposure groups (<i>P</i>＜0.05). <b>Conclusion:</b> Maternal PS-NPs exposure during gestation may affect the growth and development of fetal rats by damaging the placental barrier and produce neurotoxicity in fetal rats, causing oxidative stress and inflammatory responses in various brain regions, and smaller particle sizes and higher doses of polystyrene nanoplastic exposure have more significant neurotoxic effects on the offspring.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"760-765"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9624129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of whole body vibration on bone strength and physical fitness in elderly COPD patients complicated with osteoporosis].","authors":"Shao-Wen Chen,&nbsp;Jun Yi","doi":"10.12047/j.cjap.6341.2022.125","DOIUrl":"https://doi.org/10.12047/j.cjap.6341.2022.125","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the effects of adding whole body vibration (WBV) to routine exercise regimen of pulmonary rehabilitation (PR) on bone strength, lung function and exercise ability of elderly patients with stable chronic obstructive pulmonary disease (COPD) complicated with osteoporosis (OP). <b>Methods:</b> Thirty seven elderly patients with stable COPD were randomly divided into control group (group C, <i>n</i>=12, age: 64.6±3.8 years), conventional PR group (PR group, <i>n</i>=12, age: 66.1±4.9 years), and whole body vibration combined PR group (WP group, <i>n</i>=13, age: 65.5±3.3 years). Before intervention, X-ray and computerized tomography bone scan, bone metabolic markers, pulmonary function, cardiopulmonary exercise, 6-minute walking and isokinetic muscle strength were performed, and then intervened for 36 weeks, three times/week, among which group C subjects were given routine treatment, PR group added aerobic running and static weight resistance on the basis of routine treatment, and WP group added WBV on the basis of PR group intervention. After the intervention, the same indicators were detected. <b>Results:</b> Compared with before the intervention, the pulmonary function indexes of each group were significantly improved after the intervention (<i>P</i>＜0.05), and the bone mineral density and bone microstructure indexes of the patients in the WP group were also significantly improved (<i>P</i>＜0.05). Compared with group C and group PR, the bone mineral density, bone microstructure, parathyroid hormone (PTH), insulin-like growth factor-1 (IGF-1), interleukin-6 (IL-6), osteocalcin (OCN) and other bone metabolism indexes, knee flexion, peak extension torque, fatigue index and muscle strength of patients in WP group were significantly improved (<i>P</i>＜0.05). <b>Conclusion:</b> Adding WBV to the conventional PR regimen can improve the bone strength, lung function and exercise capacity of elderly patients with COPD complicated with OP, and may be able to make up for the deficiency of the current conventional PR regimen for insufficient muscle and bone stimulation.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"690-695"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9626848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Influence of marine main engine operation on characteristics of VOCs in the ambient air of the ship main cabins].","authors":"Yue Shi,&nbsp;Tao Yu,&nbsp;Hong-Qi Shi,&nbsp;Wen-Jun Leng,&nbsp;Long-Fei Chen,&nbsp;Zhu-Ge Xi,&nbsp;Ben-Cheng Lin","doi":"10.12047/j.cjap.6380.2022.144","DOIUrl":"https://doi.org/10.12047/j.cjap.6380.2022.144","url":null,"abstract":"目的: 了解某舰主机运行工况对舱室整体环境空气污染物浓度的影响,为舱室空气改善和作业人员健康保障提供基础支撑。方法: 利用标准Tenax TA吸附管采样-热脱附/气相色谱-质谱法测定主机转速分别在(55±5)、(75±5)、(110±5)、(145±5)、(170±5)r/min下某蒸汽动力舰船舱室环境空气中挥发性有机化合物(VOCs)的特征。结果: 不同工况下主机舱环境空气VOCs的组成和浓度基本一致,主要由正十一烷、正癸烷等脂肪烃,二甲苯、乙苯、BHT、苯等芳香烃,以及四氯乙烯、二氯甲烷等卤代烃组成。结论: 主机运行工况变化对主机舱环境空气VOCs特征的影响不显著,因此主机工况变化不影响面向主机舱环境空气VOCs控制的通风、净化系统设计和运行策略制定。.","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"793-796"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9629797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of fucoidan inducing impairment of human osteosarcoma cell 143B and its mechanism].","authors":"Qi-Qi Wang,&nbsp;Qiao Lin,&nbsp;Wei-Yan Shan,&nbsp;Tao Zhang,&nbsp;Yu-Rong Li,&nbsp;Yun Zhang","doi":"10.12047/j.cjap.6331.2022.135","DOIUrl":"https://doi.org/10.12047/j.cjap.6331.2022.135","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the effects of fucoidan inducing impairment of human osteosarcoma cell 143B, as well its mechanisms. <b>Methods:</b> After 143B cells were treated with different concentrations of FUC (0, 0.5, 1, 10, 100, 400, 800 μg/ml) for 48 h, the cell viability and dehydrogenase (LDH) level were detected by MTT assay and chemical colorimetry with six multiple wells for each concentration. Based on MTT results, we determined the value of IC₅₀ was 244.5 μg/ml. The follow-up experiments were divided into control group (without FUC), FUC (10 μg/ml)-treated group, FUC (100 μg/ml)-treated group, FUC (400 μg/ml)-treated group and positive group (resveratrol, 40 μmol/L). There were four multiple wells for each concentration, and each experiment was repeated at least three times. Flow cytometry was performed to detect cell apoptosis and intracellular reactive oxygen species (ROS) level; acridine orange (AO) staining and lyso-tracker red staining were used to observe the autophagolysosome formation; chemical colorimetric analysis was performed to determine malondialdehyde (MDA) content and the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px); Western blot was used to detect protein expressions of nuclear factor E2-associated factor 2 (Nrf2), heme oxygenase 1 (HO-1) and autophagy-associated proteins including microtubule-associated light chain protein 3 (LC-3), Atg7, Beclin-1 and p62. <b>Results:</b> Compared with control group, the cell viability was decreased significantly in FUC (100～400 μg/ml)-treated groups (<i>P</i>＜0.01); LDH levels in the supernatant (<i>P</i>＜0.05 or <i>P</i>＜0.01), the percentage of cell apoptosis (<i>P</i>＜0.01), intracellular ROS level and MDA content (<i>P</i>＜0.01) were increased remarkably; protein expressions of Atg7 and Beclin-1 were upregulated (<i>P</i>＜0.05 or <i>P</i>＜0.01); the conversion from LC-3I to LC-3II was significant (<i>P</i>＜0.01) together with elevation of autophagolysosome formation (<i>P</i>＜0.05 or <i>P</i>＜0.01); while the activities of SOD and GSH-Px and protein expressions of Nrf2, HO-1 and p62 were decreased remarkably (<i>P</i>＜0.05 or <i>P</i>＜0.01). <b>Conclusion:</b> FUC (100～400 μg/ml) treatment induces oxidative damage and autophagic death in osteosarcoma 143B cells.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"739-744"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9618060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of Bosutinib on cerebral ischemia/reperfusion injury in rats].","authors":"Yi Zhang,&nbsp;Chao Wu,&nbsp;Qi Zhang,&nbsp;Yyu Kong,&nbsp;Xiao-Qian Bian,&nbsp;Ying Wang,&nbsp;Shu Li","doi":"10.12047/j.cjap.6327.2022.146","DOIUrl":"https://doi.org/10.12047/j.cjap.6327.2022.146","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the effects of bosutinib on the early stage of cerebral ischemia-reperfusion injury in rats. <b>Methods:</b> Forty Sprague-Dawley rats were randomly divided into four groups (random number method), 10 rats in each group; sham group (control group): only neck vessels were isolated without other treatments; MCAO (model group): the rat brain ischemia/reperfusion injury model was made by a modified wire bolus method,ischemia for 2 h followed by reperfusion for 24 h; DMSO group (solvent group): DMSO ( 0.752 ml/kg) was injected into the tail vein one day before the experiment, brain ischemia 2 h reperfusion for 24 h; Bosutinib group (intervention group): one day before the experiment, the tail vein was injected with Bosutinib (4 mg/kg), brain ischemia 2 h reperfusion for 24 h. After 24 h of ischemia reperfusion, neurological function score was performed; brain infarct area was calculated after staining with TTC; SIK2 was detected by Western blot; the contents of TNF-α and IL-6 in brain tissue were detected by ELISA. <b>Results:</b> Compared with the sham group, the neurological function scores, the infarct volume percentages and the levels of inflammatory factors IL-6 and TNF-α of the MCAO and DMSO groups were increased significantly (<i>P</i>＜0.05 or <i>P</i>＜0.01). Compared with the MCAO and DMSO groups, the above mentioned indexes of the bosutinib group were all decreased significantly (<i>P</i>＜0.05 or <i>P</i>＜ 0.01). Compared with sham group, the expression levels of SIK2 protein in MCAO and DMSO groups had no significant changes(<i>P</i>＞ 0.05); compared with the MCAO and DMSO group, the expression level of SIK2 protein in the bosutinib group was decreased significantly (<i>P</i>＜0.05). <b>Conclusion:</b> Bosutinib reduces cerebral ischemia-reperfusion-induced injury, and its possible mechanism is related to the decreased expression of SIK2 protein and inflammatory factors.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"803-806"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9618061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Establishment of primary liver cancer model in mice].","authors":"Jin-Jin Wang,&nbsp;Xue-Ying Li,&nbsp;Jin-Ke Yi,&nbsp;Bei-Ling Zhao,&nbsp;Hui-Min Huang,&nbsp;Ying Wei","doi":"10.12047/j.cjap.6367.2022.149","DOIUrl":"https://doi.org/10.12047/j.cjap.6367.2022.149","url":null,"abstract":"<p><p><b>Objective:</b> Three modeling methods were used to establish a mouse primary liver cancer model, and compared them to find a more optimal modeling method. <b>Methods:</b> Forty 15-day-old C3H/HeN male mice were randomly divided into groups I-IV, 10 mice in each group. Group Ⅰ were not treated; Group Ⅱ were intraperitoneally injected with 25 mg/kg diethylnitrosamine (DEN) once; Group Ⅲ were intraperitoneally injected with 100 mg/kg DEN once; Group Ⅳ were intraperitoneally injected with 25 mg/kg DEN once and followed by another intraperitoneal injection of 100 mg/kg DEN at 42 days of age. The mortality of mice in each group was analyzed. At the 18th week of modeling, blood was collected from eyeballs after anesthesia, and liver was taken from abdominal cavity after neck was broken. The appearance of liver, the number of cancer nodules and the incidence of liver tumor were observed. The histopathological changes of liver were observed by HE staining. The serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were detected. <b>Results:</b> At the 18th week of modeling, compared with the group I, serum levels of ALT and AST in groups II-IV were increased significantly (<i>P</i>＜0.05); The number of cancer nodules and the incidence of tumors in the surviving mice of groups III and IV were also increased significantly (<i>P</i>＜0.05). At the 18th week of modeling, no mice died in both groups I and II, and the incidence of liver cancer was 0%; The incidence of liver cancer in surviving mice in both groups III and IV was 100%, but the mortality rate of mice in group III was as high as 50%, and that in group IV was only 20%. <b>Conclusion:</b> C3H/HeN male mice can successfully establish a mouse liver cancer model by intraperitoneal injection of 25 mg/kg of DEN once at the age of 15 days and another intraperitoneal injection of 100 mg/kg of DEN once at the age of 42 days with short cycle and low mortality, which is an ideal method to establish a primary liver cancer model.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"820-823"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9624122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of adipokine chemerin on the improvement of islet function in diabetic mice by aerobic exercise and its mechanisms].","authors":"Qi-Long Zhang,&nbsp;Jing Qu,&nbsp;Xiao-Hui Wang","doi":"10.12047/j.cjap.6359.2022.124","DOIUrl":"https://doi.org/10.12047/j.cjap.6359.2022.124","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the effects of adipokines chemerin on the improvement of islet function caused by exercise in mice with diabetes, and the possible mechanism of glucagon-like peptide 1 (GLP-1). <b>Methods:</b> Male ICR mice were randomly divided into a control group fed with normal diet (Con, <i>n</i>=6) and a diabetic modeling group fed with 60% kcal high-fat diet (<i>n</i>=44). After 6 weeks, the diabetic modeling group was once given a fasting intraperitoneal injection of streptozotocin (100 mg/kg). The successfully modeled mice were divided into diabetes group (DM), diabetes plus exercise group (EDM), and diabetes plus exercise and exogenous chemerin group (EDMC), 6 in each group. Mice in exercise groups participated in a six-week modest intensity treadmill running exercise with a gradually increased load. Mice in the EDMC group were intraperitoneally injected with exogenous chemerin (8 μg/kg) from the 4th week of the exercise period, six days per week, and one time per day. And the other groups were untreated. Adipose chemerin knockout mice were constructed. Then they and the control mice were divided into 6 groups (<i>n</i>=4): Normal diet control group (Con-ND), Normal diet chemerin knockout heterozygote mice group (Chemerin(+/-)-ND), Normal diet chemerin knockout homozygotes mice group(Chemerin(-/-)-ND), High-fat diet control group (Con-HFD), High-fat diet chemerin knockout heterozygote mice group (Chemerin(+/-)-HFD), High-fat diet chemerin knockout homozygotes mice group (Chemerin(-/-)-HFD). They were fed with normal or high-fat diet for 11 weeks and oral glucose tolerance test (OGTT) was conducted. After the mice of each group were executed under anesthesia, the samples such as pancreas and colon were collected. Fasting blood glucose (FBG) and fasting insulin (FINS) levels in mice were measured, and the insulin resistance index (HOMA-IR) was calculated. HE staining was used to observe the structure of islets. ELISA was used to detect the GLP-1 level in serum. The mRNA levels of proglucagon (GCG) and chemerin in the colon were measured by real-time PCR. And the protein levels of GCG and chemerin in the colon were detected by Western blot. <b>Results:</b> Compared with the DM group, the vacuolar degeneration and shrinkage of islet cells in the EDM group were reduced, the islet structure was improved, while the levels of FINS, HOMA-IR and FBG were decreased significantly (<i>P</i>＜0.05 or <i>P</i>＜0.01). The colon and serum chemerin levels were decreased significantly(<i>P</i>＜0.05), while the colonic GCG mRNA and protein levels were increased significantly (<i>P</i>＜0.05 or <i>P</i>＜0.01). Compared with the EDM group, the islet cells in the EDMC group were shrunken, with unclear borders. The structure of the islets was damaged, and the levels of FINS, HOMA-IR and FBG were increased significantly (<i>P</i>＜0.01), while the mRNA and protein levels of GCG were decreased significantly (<i>P</i>＜0.05 or <i>P</i>＜0.01). Co","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"682-689"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9629794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}