[Synergistic effects of bicuculline combined with early running on the recovery of brain injury in rats].

Q4 Medicine

Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology Pub Date : 2022-11-01 DOI:10.12047/j.cjap.6379.2022.127

Nai-Hong Liu, Dian-Qing Wang, Zhi-Feng Peng

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引用次数: 0

Abstract

目的: 评估γ-氨基丁酸(GABA)受体拮抗剂荷包牡丹碱联合早期跑步运动对大鼠脑损伤后运动功能恢复协同效应。方法: Wistar大鼠随机分为Sham组、缺血/再灌注(I/R)组、荷包牡丹碱+I/R组、运动+I/R组和荷包牡丹碱+运动+I/R组(联合处理组),每组10只大鼠。除Sham组外,其余大鼠均行大脑中动脉阻塞(MCAO)诱导I/R。I/R 2 d后,荷包牡丹碱+I/R组和联合处理组大鼠腹腔注射荷包牡丹碱,连续注射5 d;运动+I/R组和联合处理组大鼠在跑步机上连续跑5 d,每次30 min。I/R 7 d后,采用旋转杆试验和错步试验评估各组大鼠运动功能;采用TTC方法评估各组大鼠脑梗死体积;采用ELISA及Western blot方法检测各组大鼠大脑皮层和脊髓中脑源性神经营养因子(BDNF)、GAP-43、突触素和Nogo-A蛋白表达。结果: 与Sham组相比,I/R组大鼠运动功能受损,脑梗死体积增加(所有P＜0.05);与I/R组相比,荷包牡丹碱+I/R组、运动+I/R组和联合处理组大鼠运动功能改善,脑梗死体积减少(所有P＜0.05),而且联合处理组大鼠运动功能改善和脑梗死体积减少优于其他两组(P均＜0.05)。与I/R组相比,荷包牡丹碱+I/R组、运动+I/R组和联合处理组大鼠大脑皮层和脊髓BDNF蛋白表达增加(P均＜0.05);荷包牡丹碱+I/R组和联合处理组大鼠脊髓突触素和GAP-43蛋白表达增加(P均＜0.05)。此外,联合处理组大鼠脊髓Nogo-A蛋白表达高于其他所有组(P均＜0.05)。结论: 荷包牡丹碱联合早期跑步运动可更有效促进脑损伤后运动功能恢复,可能与脊髓中轴突生长和抑制性修饰有关。.

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[双卡兰联合早期跑步对大鼠脑损伤恢复的协同作用]。

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