Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology最新文献

{"title":"[Effects of hypoxic training on gene expressions of p53 and its regulated mitochondrial aerobic metabolic signaling pathway in skeletal muscle].","authors":"Jie Li, Li-Li Zhao","doi":"10.12047/j.cjap.6314.2022.105","DOIUrl":"https://doi.org/10.12047/j.cjap.6314.2022.105","url":null,"abstract":"目的: 探讨低氧训练对大鼠骨骼肌p53及其调控的线粒体有氧代谢信号通路基因表达的影响,以及对线粒体有氧氧化供能能力的影响。方法: 30只雄性Wistar大鼠随机分为3组(n=10),低住低练组(LoLo)、高住高练组(HiHi)和高住高练低训组(HiHiLo)。以当地海拔1 500 m为常氧环境,模拟海拔3 500 m为低氧环境,各组大鼠按训练方案训练5周后,取股四头肌行匀浆及提取线粒体。Real-time PCR检测p53、细胞色素c氧化酶合成2(SCO2),细胞色素c氧化酶亚基Ⅰ(COXⅠ)和谷氨酰胺酶2(GLS2) mRNA表达,Westem blot检测 p53、SCO2、COXⅠ和GLS2蛋白表达;ELISA测定α-酮戊二酸脱氢酶(α-KGDHC),细胞色素c氧化酶(COX)及ATP合酶(ATP synthase)活性。结果: ①与LoLo 组比较,HiHi和HiHiLo组p53 mRNA水平显著升高(P＜ 0.01),HiHiLo组p53蛋白表达水平显著下降(P＜0.01);HiHi和HiHiLo组SCO2 mRNA水平和蛋白表达水平均显著升高(P＜ 0.01);HiHiLo组COXⅠmRNA水平显著升高(P＜0.01),HiHi组显著降低(P＜0.01),HiHi组COXⅠ蛋白表达水平显著升高(P＜ 0.05);HiHi和HiHiLo组GLS2 mRNA水平均显著升高(P＜0.05,P＜0.01),HiHiLo组GLS2蛋白表达水平显著下降(P＜0.01)。②与LoLo组比较,HiHi组ɑ-KGDHC和COX活性均显著降低(P＜0.01,P＜0.05),HiHiLo组均显著升高(P＜0.01);HiHi和HiHiLo组ATP synthase总活性和特异性活性均显著升高(P＜0.01)。结论: 高住高练低训上调大鼠骨骼肌p53及其调控的线粒体有氧代谢信号通路相关因子基因转录水平,提高骨骼肌线粒体有氧氧化供能能力。.","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 5","pages":"564-568"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9416161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of GLAST gene knockout on phenotype and hearing in mice].","authors":"Fang-Shan Wu,&nbsp;Ke-Feng Ma,&nbsp;Peng-Fang Zheng,&nbsp;Xiao-Jun She,&nbsp;Hong-Tao Liu,&nbsp;Qing-Feng Zhai,&nbsp;Bo Cui","doi":"10.12047/j.cjap.6363.2022.092","DOIUrl":"https://doi.org/10.12047/j.cjap.6363.2022.092","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of glutamate aspartate transporter (GLAST)deletion on the normal auditory function of mice.</p><p><strong>Methods: </strong>We hybridized GLAST^+/- mice with C57BL/6J background and identified the genotypes of their offspring by agarose gel electrophoresis. 9-10-week-old mice were selected to detect the expression of GLAST protein in the cochlea by immunofluorescence staining and to verify the knockout results(<i>n</i>=3). The changes in weight from 7 days to 30 days after birth and the 30-day body length of male and female mice were compared(<i>n</i>=8). The auditory brainstem response(ABR) was used to detect the auditory threshold and the amplitude of wave I in 9-10-week-old male and female mice(<i>n</i>=5).</p><p><strong>Results: </strong>Male GLAST^-/- mice had shown significantly lower weight and body length compared to male GLAST^+/+ and GLAST^+/- mice(<i>P</i>＜0.01), and male GLAST^-/- mice showed significant differences compared to GLAST^+/+ from P7 to P30 statistical time. Male GLAST^-/- mice exhibited a significant reduction in weight after P15 compared to male GLAST^+/- mice. In contrast, no significant differences in weight and body length were observed in female GLAST^-/- mice compared with female GLAST^+/+ and GLAST^+/- mice. There was no difference in the hearing threshold detected by ABR between the three genotypes in both male and female mice, but the amplitude of wave I in GLAST^-/- mice was significantly lower than that in male GLAST^+/+ mice(<i>P</i>＜0.01). In contrast, the amplitude of wave I in females was reduced throughout the stimulus intensity but was most significant only at high-intensity stimulation (e.g.80 dB, 90 dB) (<i>P</i>＜0.05).</p><p><strong>Conclusion: </strong>GLAST knockout affects the normal growth and development of male mice, and decreases the amplitude of wave I, but do not change the threshold, suggesting that GLAST knockout may lead to synaptic pathological changes, and there are gender differences in this effect.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 5","pages":"491-496"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9423082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of heavy-load exercise on skeletal muscle cells apoptosis and mechanisms of mitochondrial apoptosis in rats].","authors":"Xiao-Qin Zhao,&nbsp;Jia-Qi You,&nbsp;Xiao-Ran Liu,&nbsp;Jun-Zhi Sun,&nbsp;Jun-Ping Li,&nbsp;Rui-Yuan Wang","doi":"10.12047/j.cjap.6319.2022.106","DOIUrl":"https://doi.org/10.12047/j.cjap.6319.2022.106","url":null,"abstract":"<p><strong>Objective: </strong>To analyze the molecular mechanisms of skeletal muscle cells apoptosis induced by heavy-load exercise with Omi as the entry point.</p><p><strong>Methods: </strong>One hundred and twenty-six adult SD rats were randomly divided into five groups: control group(C), eccentric exercise group (E), simple blocking group (U), DMSO group (D) and exercise block group (EU). In addition to the C group, the other four groups were randomly divided into 0 h after experiment, 12 h after experiment, 24 h after experiment, 48 h after experiment and 72 h after experiment with 6 rats in each group. E and EU group were submitted to a heavy-load exercise on a treadmill down a 16° decline, 16 m/min for 90 minutes. U, D and EU group were one-time intervened with drugs. U and EU groups were intraperitoneally injected with 1.5 μmol/kg ucf-101, D group were intraperitoneally injected with 1.5 μmoL/kg 0.5% DMSO. The rats were sacrificed in batches at different time points after experiment, then the soleus were saved to detect the Caspase-3,-8,-9,-12 activities and protein expressions of Omi and XIAP.</p><p><strong>Results: </strong>Compared with group C, the mitochondrial distribution and morphology appeared the typical ultrastructure pathological changes, the opening degree of MPTP was increased significantly (<i>P</i>＜0.01) or (<i>P</i>＜0.05), protein expressions of Omi and XIAP were increased significantly (<i>P</i>＜0.01 or <i>P</i>＜0.05), the activities of Caspase-9 and Caspase-3 were increased significantly (<i>P</i>＜0.01 or <i>P</i>＜0.05) in group E. Compared with group C, there was no significant difference in XIAP protein and caspase-9, - 3 activities in group U and Group D. The change trend of XIAP protein and Caspase-9, - 3 activities was the same as those between EU group and E group, but the change range of XIAP protein in EU group was significantly higher than that in E group (<i>P</i>＜0.01), and the change ranges of caspase-9, - 3 activities in EU group were significantly lower than those in E group (<i>P</i>＜0.01).</p><p><strong>Conclusion: </strong>A single heavy-load exercise can induce changes in the mitochondria morphology and structure in rats, open the high permeability of MPTP, and improve the expression of Omi protein, then through its downstream XIAP-Caspase pathway, start the mitochondrial apoptosis pathway mediated by caspase-9, and finally lead to myocyte apoptosis. The inhibition of Omi can reduce the cell apoptosis level of motor induced skeletal muscle cells.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 5","pages":"569-576"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9416158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Down-regulation of MDR1 gene expression by CRISPRi to enhance the sensitivity of lung adenocarcinoma A549/DDP cells to cisplatin].","authors":"Kai Liu,&nbsp;Xin-di Sun,&nbsp;Wei-Wei Zhang,&nbsp;Qing-Zhu Yang,&nbsp;Xin Huang,&nbsp;Shu-Li Shao","doi":"10.12047/j.cjap.6342.2022.109","DOIUrl":"https://doi.org/10.12047/j.cjap.6342.2022.109","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of down-regulating MDR1 gene expression by CRISPRi on enhancing the sensitivity of lung adenocarcinoma A549/DDP cells to cisplatin.</p><p><strong>Methods: </strong>The potential CRISPRi interference sites on the MDR1 gene promoter were predicted by bioinformatics software, and the interference fragments were designed and constructed. The mRNA and protein expression levels of MDR1 gene in each group of cells were detected by qRT-PCR and Western blot methods, and the recombinant vectors with high interference efficiency were screened. Human lung cancer A549/DDP cells were divided into three groups: A549/DDP, Scrambed and sgRNA-MDR1-1, with three multiple holes in each group. After each vector was transfected into the cells for 48 h, the efflux of cells in each group was detected by flow cytometry, the IC₅₀ value of cells in each group was detected by MTT method, and the cell morphology of cells treated with cisplatin was observed under laser confocal microscope.</p><p><strong>Results: </strong>After sequencing and comparison, two kinds of CRISPRi recombinant vectors interfering with MDR1 gene transcription were constructed successfully. After transfection of A549/DDP cells, the mRNA and protein levels of MDR1 gene in all transfection groups were decreased significantly (<i>P</i>＜ 0.01). Among them, the interference efficiency of sgRNA-MDR1-1 was the highest, and the interference efficiency of mRNA and protein was 60% and 51%, respectively. After transfection of sgRNA-MDR1-1 vector, compared with the control group, the efflux ability of cells was decreased (<i>P</i>＜0.01), the IC₅₀ value of cells to cisplatin was decreased significantly (<i>P</i>＜0.01), and the intracellular chromatin gathered and marginalized, and apoptotic bodies appeared.</p><p><strong>Conclusion: </strong>CRISPRi interference with MDR1 gene in drug-resistant A549/DDP cells can significantly enhance the sensitivity to cisplatin.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 5","pages":"590-594"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9416160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of rhein on gastric cancer cells HGC-27 apoptosis and its mechanisms].","authors":"Yi-Ting Chen,&nbsp;Zhi-Heng Chu,&nbsp;Jin-Wen Fu,&nbsp;Jia-Ying Tao,&nbsp;Jia-Yu Chen","doi":"10.12047/j.cjap.6320.2022.108","DOIUrl":"https://doi.org/10.12047/j.cjap.6320.2022.108","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of rhein on proliferation and apoptosis of gastric cancer cell line HGC-27 and its related mechanisms.</p><p><strong>Methods: </strong>Human gastric cancer cells HGC-27 were treated with 0, 5, 10 or 20 mg/L rhein respectively for 24, 48 and 72 h in vitro, three duplicate wells were set in each group. The proliferation activity of HGC-27 cells was detected with CCK-8 method, the growth status of HGC-27 cells was observed by small high-content microscope, hoechst staining was used to analyze the karyotype of HGC-27 cells. Mitochondrial membrane potential was detected by JC-1 staining and flow cytometry, cell cycle was analyzed with flow cytometry, the levels of mRNA transcribing of <i>bcl-2,</i> bax, caspase-3, <i>jak1,</i> <i>jak2,</i> <i>stat3</i> and <i>notch</i> genes were investigated with RT-qPCR method. Protein expressions were determined by Western blot.</p><p><strong>Results: </strong>Compared with HGC-27 cells treated with 0 mg/L rhein, HGC-27 cells treated with 5, 10 and 20 mg/L rhein for 24 h showed decreased mitochondrial membrane potential ( <i>P</i>＜0.01), the cell proliferation activity was inhibited and apoptosis was induced. The effects were enhanced with the increase of rhein concentration and the extension of treatment time, but the cell cycle did not change significantly, and the expressions of bcl-2, jak1, jak2, stat3 and notch genes were down-regulated. The expression levels of bax and caspase-3 genes were increased significantly ( <i>P</i>＜0.01).</p><p><strong>Conclusion: </strong>Rhein can induce apoptosis of HGC-27 cells by influencing NOTCH/JAK/STAT signaling pathway, and has anti-gastric cancer effect.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 5","pages":"584-589"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9416162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of ferulic acid on inflammation and autophagy levels in glomerular mesangial cells induced by high glucose].","authors":"Qing Fang,&nbsp;Ru-Yu Ma,&nbsp;Ying-Hao He,&nbsp;Min-You Qi","doi":"10.12047/j.cjap.6305.2022.085","DOIUrl":"https://doi.org/10.12047/j.cjap.6305.2022.085","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the protective effects and possible mechanisms of ferulic acid on diabetic nephropathy by observing the effects of ferulic acid on the level of inflammation and autophagy in glomerular mesangial cells induced by high glucose.</p><p><strong>Methods: </strong>SV40 MES 13 cells were cultured and randomly divided into the following groups: normal group (Control, 5.6 mmol/L glucose), mannitol group (Man, 30 mmol/L mannitol), high glucose group (HG, 30 mmol/L glucose), ferulic acid group (FA, 30 mmol/L glucose + 12.5, 25, 50, 100, 200 μmol/L ferulic acid), and the proliferation of SV40 MES 13 cells in each group was observed by MTT method. The levels of tumour necrosis factor-α (TNF-α), monocyte chemotactic protein-1 (MCP-1) and interleukin 1β(IL-1β)in cell supernatant were determined by enzyme-linked immunosorbent assay (ELISA). The expressions of NLRP3, IL-1β, LC3-II/I and p62 proteins in SV40 MES 13 cells were detected by Western blot.</p><p><strong>Results: </strong>①The proliferative activity of SV40 MES 13 cells was significantly higher in the HG group compared to the control group (<i>P</i>＜0.01), while the proliferative activity of SV40 MES 13 cells was decreased to different degrees in the FA group compared to the HG group (<i>P</i>＜0.05～0.01). ②Compared to the control group, the levels of TNF-α, MCP-1 and IL-1β were increased significantly in the cell supernatant of HG group (<i>P</i>＜0.01). Compared with the HG group, the levels of TNF-α, MCP-1 and IL-1β were decreased significantly in the FA group (<i>P</i>＜0.01). ③Compared with the control group, LC3-II/Ⅰ protein expression was decreased in the HG group, while the levels of p62, NLRP3 and IL-1β protein were increased significantly (<i>P</i>＜0.01). Compared with the HG group, the expression of LC3-II/Ⅰ protein was elevated significantly (<i>P</i>＜0.05) in the FA group, while the levels of p62, NLRP3 and IL-1β protein in the FA group were decreased significantly (<i>P</i>＜ 0.01).</p><p><strong>Conclusion: </strong>FA can inhibit the abnormal proliferation of SV40 MES 13 cells induced by high glucose. FA can protect glomerular mesangial cells by inhibiting inflammation and increasing the level of autophagy.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 5","pages":"453-457"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9423077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Protective effects of Sphingosine-1-phosphate (S1P) on hypertrophic response in H9c2 cardiomyocytes].","authors":"Hui Yan,&nbsp;Hu Zhao,&nbsp;Lun Li","doi":"10.12047/j.cjap.6281.2022.095","DOIUrl":"https://doi.org/10.12047/j.cjap.6281.2022.095","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of sphingosine-1-phosphate (S1P) on cardiac hypertrophic response in H9c2 cells.</p><p><strong>Methods: </strong>H9c2 cells were randomly divided into four groups: normal control group, S1P (1 μmol/L) treated group, Phenylephrine (PE) (100 μmol/L) treated group, PE (100 μmol/L) treated group combined with S1P (1 μmol/L) treatment. Each group has 3 duplicated wells. After 24 hours, the size of H9c2 cells in each group was detected by Actin-Trakcer Green immunofluorescence staining. Transcriptional levels of hypertrophic markers ( ANP, BNP and β-MHC) in H9c2 cells were determined by real-time PCR. Western blot was performed to examine the expression level of ANP in each group. Then H9c2 cells were randomly divided into five groups: normal control group, PE (100 μmol/L) treated group, PE (100 μmol/L) with S1P low-dose (0.1 μmol/L) treated group, PE (100 μmol/L) with S1P middle-dose (1 μmol/L) treated group and PE (100 μmol/L) with S1P high-dose (10 μmol/L) treated group. Each group has 3 duplicated wells. After 24 hours, Western blot was performed to examine the expressions of phosphorylated Janus kinase 2 (JAK2) and signal transducers and activators of transcription 3 (STAT3) under low, medium and high concentrations of S1P. Each experiment was repeated three times.</p><p><strong>Results: </strong>Compared with normal control group, the surface area of H9c2 cells in PE group was increased significantly (<i>P</i>＜0.05), meanwhile, the transcription levels of ANP, BNP and β-MHC were increased significantly (all <i>P</i>＜0.05), and the expression of ANP was also increased significantly (<i>P</i>＜0.05) in PE group. While compared with PE group, the surface area of H9c2 cells in PE + S1P group was decreased significantly (<i>P</i>＜0.05), the transcription levels of ANP, BNP and β-MHC and the expression of ANP were also decreased significantly (all <i>P</i>＜0.05) in PE + S1P group. After treated with PE and different concentrations of S1P, the expressions of p-JAK2 and p-STAT3 were increased significantly compared with the normal control group and PE group (<i>P</i>＜0.05), in a dose-dependent manner.</p><p><strong>Conclusion: </strong>S1P could protect H9c2 cells against hypertrophic response induced by PE, which may be achieved by activating JAK2/STAT3 signal pathway.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 5","pages":"510-514"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9428311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Protective effects of hydroxysafflower yellow A on pulmonary fibrosis in mice].","authors":"Zhi-Hua Luan,&nbsp;Yan-Ming Wei,&nbsp;Yin-Xia Chang","doi":"10.12047/j.cjap.6335.2022.103","DOIUrl":"https://doi.org/10.12047/j.cjap.6335.2022.103","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effect of hydroxysafflower yellow A (HSYA) on pulmonary fibrosis induced by bleomycin in mice and transforming growth factor β 1(TGF-β1) /Smad signal transduction pathway regulation.</p><p><strong>Methods: </strong>The pulmonary fibrosis model was prepared by intranasal injection of bleomycin 50 μl (15 mg/kg). ICR mice were randomly divided into control group, model group, HSYA group(6 mg/kg) and dexamethasone (Dex) group(3 mg/kg), with 15 mice in each group. From the next day of modeling, HSYA and Dex groups were intraperitoneally injected with corresponding drugs, while the control group and model group were intraperitoneally injected with the same volume of normal saline, once a day, for 28 consecutive days. After 4 weeks, the mice were sacrificed and the lungs were collected. HE and Masson staining were used to observe the pathological damage of lung tissue; Immunohistochemistry, RT-qPCR and Western blot were used to detect the expressions of TGF-β1/Smad signaling pathway in lung tissues.</p><p><strong>Results: </strong>Compared with the control group, the model group showed severe alveolitis and pulmonary fibrosis. The mRNA and protein expressions of TGF-β1 and Smad3 in lung tissues were increased significantly (<i>P</i>＜0.01), while the mRNA and protein expressions of Smad7 were decreased significantly (<i>P</i>＜0.01). Compared with the model group, the degree of alveolitis and pulmonary fibrosis in the HSYA and Dex groups was reduced significantly. The mRNA and protein expressions of TGF-β1 and Smad3 in lung tissues of HSYA and Dex groups were decreased significantly (<i>P</i>＜0.01), while the mRNA and protein expressions of Smad7 were increased significantly(<i>P</i>＜0.01).</p><p><strong>Conclusion: </strong>HSYA can alleviate the pathogenesis of pulmonary fibrosis, and its mechanism may be related to the regulation of TGF-β1/Smad signaling pathway.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 5","pages":"555-558"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9428315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of SIRT1 in amygdala on chronic restraint stress-induced depression-like behaviors in rats].","authors":"Cai-Yun Huang,&nbsp;Na-Na Chen,&nbsp;Fei Zhou,&nbsp;Hong-Mei Zhang,&nbsp;Xiao-Rong Yang","doi":"10.12047/j.cjap.6306.2022.086","DOIUrl":"https://doi.org/10.12047/j.cjap.6306.2022.086","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of silent information regulator 1 (SIRT1) in amygdala on depression-like behaviors in rats using chronic restraint stress (CRS) as a model of depression.</p><p><strong>Methods: </strong>Sixty male SD rats were randomly divided into six groups (<i>n</i>=10 per group): control group (Control), chronic restraint stress group (CRS), CRS + fluoxetine-treated group (CRS + FLU), CRS + saline-treated group (CRS + NaCl), CRS + SIRT1-overexpression group (CRS + AAV-SIRT1), and CRS + empty vector group (CRS + AAV-EGFP). Except for the control group, rats from the other groups were exposed to chronic restraint stress for 21 days. After the modeling, rats in fluoxetine-treated group and saline-treated group were, respectively, treated with fluoxetine (10 mg/kg) or saline (10 mg/kg) by gavage every day for 3 weeks; AAV-SIRT1 or AAV-EGFP was, respectively, stereotaxically injected into the amygdala of rats in SIRT1-overexpression group and empty vector group, and the virus was expressed for 3 weeks. Rats in normal control group and CRS model group were not given any drug treatment. The depression-like behaviors of rats in each group were evaluated by sugar preference test (SPT), open field test (OFT) and forced swimming test (FST). SIRT1 expression in amygdala of rats was assessed by using immunoblot blotting. The number of SIRT1-positive cells in amygdala of rats was detected by immunofluorescence technique.</p><p><strong>Results: </strong>Compared with the normal control group, the level of SIRT1 protein and the number of SIRT1⁺ cells in amygdala of the CRS-exposed rats were decreased significantly (<i>P</i>＜0.01), and CRS-exposed rats showed a significant decrease in sucrose preference (<i>P</i>＜0.01), less total horizontal distance (<i>P</i>＜0.01) and less time entered the center field (<i>P</i>＜0.01) in the OFT, a significant increase in the immobility time of the FST (<i>P</i>＜0.01). Fluoxetine treatment (<i>P</i>＜0.05, <i>P</i>＜0.01) or SIRT1 overexpression (<i>P</i>＜0.01) partially reversed the down-regulation of SIRT1 protein and SIRT1⁺ cells in amygdala of CRS-exposed rats and significantly improved the depression-like behaviors of CRS rats.</p><p><strong>Conclusion: </strong>Fluoxetine treatment partially reversed the down-regulation of SIRT1 level and the number of SIRT1⁺ in CRS rats, and significantly improved the depression-like behaviors. The antidepressant effect of fluoxetine treatment may be related to the up-regulation of SIRT1 in the amygdala of CRS-exposed rats.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 5","pages":"458-463"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10014984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Protective effects of <i>Polygonatum odoratum</i> polysaccharides on alcohol-induced injury of HepG2 cells and its mechanisms].","authors":"Qi Zhu,&nbsp;Ya-Wen Wu,&nbsp;Xiao-Hui Wang,&nbsp;Geng-Xi Li","doi":"10.12047/j.cjap.6287.2022.047","DOIUrl":"https://doi.org/10.12047/j.cjap.6287.2022.047","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the protective effects of <i>Polygonatum odoratum</i> polysaccharides (POP) on alcohol-induced injury of HepG2 cells and its potential molecular mechanisms. <b>Methods:</b> After screening the appropriate concentration of alcohol-treated HepG2 cells and the intervention concentration of POP by MTT method, HepG2 cells were divided into three groups according to different intervention concentrations (200 μg/L, 400 μg/L and 600 μg/L) of POP, and the blank group without POP. After pretreated for 1 h, HepG2 cells were treated with 4% alcohol for 24 h. The activities of intracellular alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured, and the levels of intracellular reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF- α) were measured. The protein expressions of Kelch-like epichlorohydrin-associated protein-1 (Keap1), phosphorylated nuclear factor E2-related factor 2 (p-Nrf2), phosphoamide adenine dinucleotide quinone oxidoreductase -1 (NQO1), B lymphocyte tumor-2 (Bcl-2), Bcl-2-associated X protein (Bax) and caspase 3 were detected. <b>Results:</b> Compared with the HepG2 cells treated with 4% alcohol, POP at the various concentrations could effectively down-regulate the activities of ALT and AST in HepG2 cells induced by alcohol (<i>P</i>＜0.05). The levels of IL-1β and TNF-α in the 200 μg/L POP treated group were decreased significantly (<i>P</i>＜0.05), while the level of GSH was increased significantly (<i>P</i>＜0.01). The levels of ROS, MDA, IL-1β and TNF-α in the 400 μg/L and 600 μg/L POP treated groups were decreased significantly (<i>P</i>＜0.05 or <i>P</i>＜0.01), while the GSH level was increased significantly (<i>P</i>＜0.01). POP effectively up-regulated the expressions of p-Nrf2 and NQO1 protein in HepG2 cells induced by alcohol, and also down-regulated the Bax/Bcl-2 index (<i>P</i>＜0.05), and inhibited the protein expressions of Keap1 and cleaved-caspase-3 (<i>P</i>＜0.05). <b>Conclusion:</b> POP can improve alcohol-induced oxidative stress injury in HepG2 cells by regulating the Nrf2/Keap1 pathway, thereby reducing the inflammatory index and apoptosis level of HepG2 cells. Among them, 400 μg/L and 600 μg/L POP have better intervention effects.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 3","pages":"227-232"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40351656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}