Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology最新文献

{"title":"[Effects of propranolol on biological function of human esophageal squamous cell carcinoma cells].","authors":"Qing-Ya Zhuo, He Qian, Bao-Sheng Zhao, Bo Qi, Yu-Zhen Liu","doi":"10.12047/j.cjap.6374.2022.137","DOIUrl":"https://doi.org/10.12047/j.cjap.6374.2022.137","url":null,"abstract":"Objective: To investigate the effects of propranolol on the subcutaneous tumorigenesis of esophageal squamous cell carcinoma (ESCC) cells and the proliferation, migration, cell cycle, apoptosis and autophagy of ESCC cells and its possible molecular mechanisms. Methods: The cell proliferation was detected by MTT (methyl thiazol tetrazolium) assay: ESCC Eca109, KYSE-450 and TE-1 cells were routinely cultured. PBS (Phosphate buffer saline) group (without propranolol) and treated groups (40, 60, 80, 100 μmol/L propranolol) were set up with 5 wells in each group. After treatment for 0, 24, 48, 72 h, 10 μl (5 mg/ml) of MTT was added to each well, and the absorbance was measured at 490 nm. The cell migration was tested by Transwell assay: ESCC Eca109, KYSE-450 and TE-1 cells were routinely cultured, and PBS group (without propranolol) and treated groups (40, 60 μmol/L) were set up with 2 wells in each group. Photos were taken 40 h later, and the experiment was repeated for three times before statistical analysis. The cell cycle and apoptosis were detected by flow cytometry assay: ESCC Eca109, KYSE-450 and TE-1 cells were routinely cultured. PBS group (without propranolol) and treated group (80 μmol/L) were set up, fixed, stained, and fluorescence at 488 nm was detected. The protein levels were detected by Western blot: ESCC Eca109 and KYSE-450 cells were routinely cultured. PBS group (without propranolol) and treated groups (60, 80 μmol/L) were set up followed by gel electrophoresis, wet membrane transfer, and ECL imaging. The experiment was repeated for three times and then analyzed statistically. Subcutaneous tumor formation experiment in nude mice: 10 nude mice were assigned PBS group (without propranolol) and treated group (with propranolol). Five mice in each group were inoculated with 5×106 cells/100 μl (Eca109) into the right underarm. The treated group was given a gavage of 0.4 ml/kg (6 mg/kg) every other day, and the tumor size was measured every other day for 3 weeks. After 20 days, the nude mice were dislocated and sacrificed to take tumor tissue. Result: The results showed that propranolol inhibited the proliferation of Eca109, KYSE-450 and TE-1 cells with IC50 of around 70 μmol/L for 48 h. Eca109, KYSE-450 and TE-1 cell migration was inhibited by propranolol in a dose-dependent manner (P＜0.05); Propranolol blocked the cell cycle of Eca109 in G2/M phase, blocked the cell cycle of KYSE-450 and TE-1 in G0/G1 phase, and promoted apoptosis of three kinds of cells (P＜0.05). The results of cell fluorescence showed that LC3 fluorescence intensity of TE-1 was increased after 12 h, 24 h and 36 h treatment with propranolol (P＜0.05). Western blot results showed that compared with PBS group, the protein expressions of p-mTOR, p-Akt and cyclin D1 were down-regulated, while cleaved caspase 9 level was up-regulated (P＜0.05). The results of subcutaneous tumor formation in nude mice showed that the tumor weight of PBS group was (0.91±0.05)g, and that of the ","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"754-759"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9618062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of Danzhen headache capsule on the expression of CREB and ERK in CM rats by mediated central sensitization mechanism].","authors":"Rui-Qiong Wang, Yan-Mei Ning, Guo-Tai Wu, Li-Dong DU, Zhi-Wang Wang","doi":"10.12047/j.cjap.6329.2022.140","DOIUrl":"https://doi.org/10.12047/j.cjap.6329.2022.140","url":null,"abstract":"目的: 探讨丹珍头痛胶囊对中枢敏感介导的慢性偏头痛大鼠的干预作用。方法: 将60只SD大鼠随机分为正常组、模型组、盐酸氟桂利嗪组(FNZ)、丹珍头痛胶囊(DZTT)高、中、低剂量组(1.0、0.5、0.25 g/kg)(n=10)。采用连续皮下注射硝酸甘油法制作慢性偏头痛大鼠模型,丹珍头痛胶囊给予大鼠灌胃治疗,每天1次,连续35 d。观测各组大鼠大体状态、耳红出现及消失时间、搔头次数、爬笼次数等疼痛行为学指标,测定眶周及足底的机械痛阈值,RT-PCR及Western blot技术分别检测三叉神经脊束核尾侧(TNC)组织中CREB、ERK的mRNA表达及CREB、pCREB、ERK、pERK蛋白表达水平。结果: 与正常组比较,模型组大鼠行为学评分明显升高,眶周及足底机械痛阈值逐日下降,TNC组织中CREB、ERK基因表达及CREB、pCREB、ERK、pERK蛋白表达水平均显著升高(P＜0.01);与模型组比较,DZTT、FNZ组大鼠疼痛症状明显缓解,疼痛行为学评分显著降低而眶周及足底机械痛阈值显著升高,TNC组织中CREB、ERK基因表达及CREB、pCREB、ERK、pERK蛋白表达水平均显著下降(P＜0.05,P＜0.01);与FNZ组比较,在给药第35日,DZTT高剂量组大鼠疼痛行为学评分、眶周及足底机械痛阈、TNC组织中CREB mRNA表达及ERK、pERK蛋白表达水平差异无显著性(P＞0.05)。结论: DZTT对中枢敏感介导的慢性偏头痛具有一定的治疗作用,抑制CREB、ERK的激活和磷酸化是其可能机制之一。.","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"771-775"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9629800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The antifatigue effects of Ginseng compound on mice in acute hypoxic environment].","authors":"Li Zhao, Meng Li, Bo-Hua Ma, Wen-Hui Shi, Rui Wang, Dong-Feng Yin","doi":"10.12047/j.cjap.6369.2022.120","DOIUrl":"https://doi.org/10.12047/j.cjap.6369.2022.120","url":null,"abstract":"目的: 在模拟海拔6 000 m高原低氧环境中观察人参复方对急性低氧小鼠抗疲劳的作用。方法: 将SPF级昆明小鼠分为正常组、缺氧模型组、阳性对照组(红景天)、人参复方低剂量组(1.0 g/kg)、中剂量组(2.0 g/kg)、高剂量组(3.0 g/kg),正常组和缺氧模型组灌等量生理盐水,按10 ml/kg体质量每天灌胃一次,连续灌胃给药14 d,利用特殊环境人工低压氧舱模拟海拔6 000 m建立小鼠急性低氧模型,除正常组外,其余组小鼠均置于人工低压氧舱中,历时24 h,然后进行负重游泳,记录小鼠负重游泳力竭时间,检测超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、三磷酸腺苷(ATP)、尿素氮(BUN)、肌糖原(MG)、肝糖原(Gly)、乳酸(LA)、乳酸脱氢酶(LDH)含量。结果: 与缺氧模型组相比,人参复方对小鼠负重游泳力竭时间显著延长(P＜0.05);与正常组相比,人参复方低、中、高剂量组血清中LA、Gly含量均显著升高(P＜0.05);与阳性对照组相比,人参复方高剂量组血清中LA含量显著升高(P＜0.05);与缺氧模型组相比,人参复方低、中、高剂量组血清中BUN、LA含量显著降低(P＜ 0.05),肝糖原中Gly含量显著升高(P＜0.05),人参复方中、高剂量组血清中ATP、LDH含量、肌糖原中MG含量显著升高(P＜ 0.05),MDA含量显著降低(P＜0.05),人参复方高剂量组SOD含量显著升高(P＜0.05)。结论: 人参复方可显著提高急性低氧环境中小鼠抗疲劳作用。.","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"660-663"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9626842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Interventional effects of activating SUR2B/Kir6.1-type K_ATP channels on renal cells injury and its mechanisms].","authors":"Ying Zhao,&nbsp;Hai Wang","doi":"10.12047/j.cjap.6356.2022.110","DOIUrl":"https://doi.org/10.12047/j.cjap.6356.2022.110","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the interventional effects of a new SUR2B/Kir6.1-type K_ATP Channel opener iptakalim on injury renal cells (the renal glomerular endothelial, mesangial and tubular epithelial cells) and its mechanisms. <b>Methods:</b> ①Experimental protocol: control: the cells were treated with with 0 mg/L uric acid for 24 h; model: the cells were treated with with 1 200 mg/L uric acid for 24 h; pretreatment with iptakalim: the cells were pretreated with 0.01,0.1,1,10,100 μmol/L iptakalim for 24 h prior to treatment with 1 200 mg/L uric acid for 24 h; pretreatment with glibenclamide: the cells were preincubated with/without 10 μmol/L glibenclamide for 1 h and then treated with 10 μmol/L iptakalim for 24 h followed by incubation with 1 200 mg/L uric acid for another 24 h. ②The cell viability was measured by MTT assay and flow cytometry; the protein expressions of Kir6.1 and SUR2B and nuclear translocation were detected by immunostaining; the protein expressions of Kir6.1 and SUR2B were determined by Western blot analysis; adhesion of mononuclear cells to endothelial cells were tested by fluorimetric assay; the content of MCP-1 was measured by enzyme linked-immunosorbent assay (ELISA). <b>Results:</b> The renal glomerular endothelial, mesangial and tubular epithelial cells were exposed to 1 200 mg/L uric acid for 24 h. Compared with the control group, 1 200 mg/L uric acid decreased the cell survival rates significantly (<i>P</i>＜0.01, <i>P</i>＜0.01, <i>P</i>＜0.01). Compared with the model group, pretreatment with 0.1, 1, 10, 100 μmol/L iptakalim could remarkably alleviate cellular damages of glomerular endothelium, mesangium cells induced by uric acid (<i>P</i>＜0.05, <i>P</i>＜0.01, <i>P</i>＜0.01, <i>P</i>＜0.01). The K_ATP channel blocker could clearly reduce survival rates of the renal glomerular endothelial, mesangial cells(<i>P</i>＜0.01) and markedly reverse the inhibitory effects of iptakalim on cell death (<i>P</i>＜0.05, <i>P</i>＜0.01), no obvious difference in comparison with the model group (<i>P</i>＞0.05). Compared with the model group, pretreatment with 10, 100 μmol/L iptakalim could notably attenuate cellular damages of tubular epithelial cells induced by uric acid (<i>P</i>＜0.05, <i>P</i>＜0.05). The K_ATP channel blocker could obviously damage the tubular epithelial cells (<i>P</i>＜0.01), no obvious difference in comparison with the model group (<i>P</i>＞0.05). Compared with control group, exposure of renal tubular epithelial, mesangial and glomerular endothelial cells to 1 200 mg/L uric acid for 24 h caused a significant increase in the protein expressions of Kir6.1 and SUR2B(<i>P</i>＜0.05). Compared with the model group, the overexpressions of Kir6.1 and SUR2B were suppressed in presence of iptakalim at a concentration of 10 μmol/L (<i>P</i>＜0.05). These decreases in the expressions of Kir6.1 and SUR2B were prevented by the K_ATP channel blocker, no obvious difference in comp","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"604-610"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9680481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of epithelial-mesenchymal transition on cardiac fibrosis induced by oil mist particulate matter].","authors":"Xuan Liu,&nbsp;Hui-Peng Nie,&nbsp;Huan-Liang Liu,&nbsp;Yue Shi,&nbsp;Wen-Qing Lai,&nbsp;Zhu-Ge Xi,&nbsp;Ben-Cheng Lin","doi":"10.12047/j.cjap.6351.2022.115","DOIUrl":"https://doi.org/10.12047/j.cjap.6351.2022.115","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the effects of oil-mist particulate matter (OMPM) on cardiac tissue structure fibrosis in rats and the role of epithelial-mesenchymal transition (EMT). <b>Methods:</b> Six-week-old Wistar rats (half male and half female) were randomly divided into 3 groups: control group (without OMPM exposure), low-dose exposure group (50 mg/m³) and high-dose exposure group (100 mg/m³), 18 rats in each group, with 6.5 hours per day of dynamic inhalation exposure. After 42 days of continuous exposure, cardiac tissues were collected for morphological observation; Western blot was used to detect fibrosis markers collagen I and collagen III levels, epithelial marker E-cadherin levels, interstitial markers N-cadherin, fibronectin, vimentin, alpha-smooth muscle actin (α-SMA) levels, and EMT transcription factor Twist protein levels; Real-time polymerase chain reaction (RT-qPCR) was used to detect collagen I and collagen III mRNA levels. <b>Results:</b> After OMPM exposure, myocardial cell edema and collagen fiber deposition were increased gradually with increasing exposure dose. Western blot results showed that compared with the control group, the expression levels of collagen I, collagen III, N-Cadherin, fibronectin, vimentin, α-SMA, and Twist protein were increased significantly in the low-dose exposure group and the high-dose exposure group (<i>P</i>＜0.01), and protein expression levels were higher in the high-dose exposure group than those in the low-dose exposure group (<i>P</i>＜0.01). In contrast, E-Cadherin protein expression levels were decreased significantly, and lower in the high-dose exposure group (<i>P</i>＜0.01). RT-qPCR results showed that compared with the control group, collagen I and collagen III mRNA levels were increased significantly in the low-dose exposure group and the high-dose exposure group (<i>P</i>＜0.01), and were increased with increasing exposure dose. (<i>P</i>＜0.01). <b>Conclusion:</b> OMPM may induce cardiac fibrosis in rats by promoting EMT process.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"633-637"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9680485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of silence information regulator 7 on proliferation and apoptosis of mouse renal podocytes under high glucose environment].","authors":"Min Feng,&nbsp;Ting Lin,&nbsp;Xia-Xia Chen,&nbsp;Xiao-Ling Yang,&nbsp;Qi Lyu,&nbsp;Jun-Ping Wen","doi":"10.12047/j.cjap.6366.2022.111","DOIUrl":"https://doi.org/10.12047/j.cjap.6366.2022.111","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the effects and its mechanisms of silence information regulator 7(SIRT7)on mouse renal podocytes proliferation and apoptosis under high glucose environment. <b>Methods:</b> Mouse renal podocytes cultured with high glucose and treated with different methods were divided into the following groups:control group(Control),high glucose group(HG),high glucose+transfecting with SIRT7 overexpression vetor(pcDNA3.1-SIRT7) group(SIRT7 OE+HG),high glucose+transfecting with the negative control vetor(pcDNA3.1)group(SIRT7 OE-NC+HG),high glucose+transfecting with small interfering RNA-SIRT7 (siRNA-SIRT7) group (siRNA-SIRT7+HG), high glucose+ transfecting with siRNA-SIRT7 control group (siRNA-SIRT7-NC+ HG). Viability of proliferation was examined by CCK-8 method.Rate of apoptosis was detected by flow cytometry. The level of SIRT7 mRNA expression was measured by qRT-PCR. Western blot was performed to detect the protein expression of Nephrin and key factors of Wnt/β-catenin signaling pathway. <b>Results:</b> The CCK-8 result showed that,compared with control group, the proliferative activity of mouse renal podocytes in HG group was decreased (<i>P</i>＜0.05). After transfected with SIRT7 overexpression vetor or small interfering RNA-SIRT7,compared to HG group,the cell proliferation activity was further decreased in siRNA-SIRT7 group(<i>P</i>＜0.05),but it was enhanced in SIRT7 OE+HG group (<i>P</i>＜0.05). The results of flow cytometry showed that compared with the control group, the apoptosis rate of cells in the HG group was increased (<i>P</i>＜0.05). Compared with the HG group, the apoptosis rate of cells in the siRNA SIRT7+HG group was increased significantly(<i>P</i>＜0.05), while that in the SIRT7 OE+HG group was decreased (P＜0.05). Compared with control group,the expressions of Nephrin, Wnt5a and β-catenin were inhibited in HG group (<i>P</i>＜0.05). compared to HG group,siRNA-SIRT7 could down-regulate the expression levels of Nephrin, Wnt5a and β-catenin in siRNA-SIRT7 group (<i>P</i>＜0.05), SIRT7 overexpression could up-regulate the expression levels of Nephrin, Wnt5a and β-catenin in SIRT7 OE+HG group (<i>P</i>＜0.05). <b>Conclusion:</b> The findings suggest that high glucose environment is an important factor to inhibit the proliferation and induce apoptosis of mouse renal podocytes.Overexpression of SIRT7 can reverse the effects by activating Wnt/β-catenin signaling pathway and up-regulating β-catenin expression.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"611-616"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10519353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of cigarette smoke extract on mitochondrial function of macrophages].","authors":"Jin-Shu Wei,&nbsp;Yuan Tian,&nbsp;Xiao-Ya Yu,&nbsp;Mei-Qi Guan,&nbsp;Jing-Jing Wei","doi":"10.12047/j.cjap.6370.2022.114","DOIUrl":"https://doi.org/10.12047/j.cjap.6370.2022.114","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the effects of cigarette smoke extract (CSE) on the mitochondrial function of macrophages. <b>Methods:</b> RAW264.7 macrophages were used for the experiment in this study. When the cell density was about 70%, the old culture medium was abandoned, and the 100% CSE stock solution was diluted with serum-free DMEM and FBS into 1%, 5%, 15%, 25% and 90% CSE and added to the well plate. The cell activity of RAW264.7 cells treated with CSE at different concentrations for 24 h was detected by CCK-8 method. Then the optimal CSE concentration was selected to treat cells for 0 h, 24 h, 48 h or 72 h respectively, and CCK-8 assay was used to detect the cell activity of CSE treated cells at different time groups. After the cells were treated with 0%, 5% and 25% CSE for 24 hours, cell necrosis and apoptosis was detected by Annexin V-FITC /PI staining; Mitochondrial membrane damage of RAW 264.7 was detected by mitochondrial membrane potential assay kit with JC-1; Macrophages were stained with ROS-specific dye DCFH-DA, and then Flow cytometer was used to determine the fluorescence and the proportion of ROS-positive macrophages; the enhanced ATP assay kit was used to detect the intracellular ATP concentration. <b>Results:</b> ①Compared with 0% CSE, cell viability was increased significantly in 1% CSE group (<i>P</i>＜0.01), cell viability was decreased significantly when CSE concentration was above 5% (<i>P</i>＜0.05); Macrophages were treated with 5% CSE, and cell viability was decreased significantly with the increase of treatment time (<i>P</i>＜0.01). ②Compared with 0% CSE, 5% CSE and 25% CSE mainly caused macrophage necrosis, decreased mitochondrial membrane potential, increased ROS production and decreased ATP significantly (<i>P</i>＜0.05 or <i>P</i>＜0.01), and the changes were more significant in 25% CSE treatment group(<i>P</i>＜0.05 or <i>P</i>＜0.01). <b>Conclusion:</b> CSE may affect mitochondrial function of macrophages, leading to decreased cell viability and necrosis.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"628-632"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10519359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Using changes of left cardiac functional parameters and CPET evaluated the clinical effectiveness of individualized precise exercise overall program management of chronic disease I --Analysis between groups].","authors":"Yan-Fang Zhang,&nbsp;Xing-Guo Sun,&nbsp;Ji-Nan Wang,&nbsp;Wen-Qi Tai,&nbsp;Qing-Qing Zhou,&nbsp;Ya Song,&nbsp;Jia-Hao Chen,&nbsp;Jiang Huang,&nbsp;Beng Jie,&nbsp;Fan Xu,&nbsp;Chao Shi,&nbsp;Fang Liu,&nbsp;Ye Zhang,&nbsp;Hao Li,&nbsp;You-Hong Xie","doi":"10.12047/j.cjap.0106.2022.001","DOIUrl":"https://doi.org/10.12047/j.cjap.0106.2022.001","url":null,"abstract":"<p><p><b>Objective:</b> To explore and study the clinical usefulness of continuous dynamic recording of left cardiac function changes forevaluation the improvement in patients with chronic disease after 3 months of intensive control of individualized precision exercise overall manage program. <b>Methods:</b> From 2018 to 2021, 21 patients with chronic cardiovascular and cerebrovascular metabolic diseases mainly controlled by our team were selected to complete the cardiopulmonary exercise test (CPET) and Non-invasive synchronous cardiac function detector (N-ISCFD), electrocardiogram, radial pulse wave, jugular pulse wave and cardiogram data were continuously recorded for 50s.According to the titration results under CPET and continuous functional parameters monitoring, a holistic plan with individualized moderate exercise intensity as the core was developed for 3 months of intensive management, and then N-ISCFD data collection was repeatedafter signing the informed consent. All N-ISCFD data were analyzed in the 50s according to the optimal report mode of Fuwai Hospital and 52 cardiac functional indexes were calculated. The data before and after the enhanced control were compared and the paired T-test was used to statistically analyze the changes of groups. <b>Results:</b> Twenty-one patients with chronic diseases (16 male and 5 female) were (54.05±12.77,29~75) years, BMI (25.53±4.04,16.62~31.7) kg/m².Comparison with baseline,the whole group analysis: ①The body weight, BMI, systolic blood pressure and diastolic blood pressure of patients were significantly decreased(<i>P</i>＜0.01).②CPET Peak VO₂ was (64.93±24.22, 26.96~103.48) %Pred before enhanced control, and (85.22±30.31, 43.95~140.48) %Pred after enhanced control, and increased (35.09±27.87, 0.12~129.35) % after enhanced control compared with before enhanced control. The AT, Peak VO₂/HR, Peak Work Rate, OUEP, FVC, FEV₁, FEV3/FVC% and MVV were significantly increased (<i>P</i>＜0.01) and the Lowest VE/VCO₂ and VE/VCO₂ Slope were significantly decreased(<i>P</i>＜0.01).③Core indicators of left heart function:Ejection fraction was significantly increased from (0.60±0.12,0.40~0.88) to(0.66±0.09, 0.53~0.87)(<i>P</i>＜ 0.01), by (12.39±14.90,-12.32~41.11)%. The total peripheral resistance was significantly decreased from (1579.52±425.45,779.46~2409.61) G/(cm⁴·s),to(1340.44±261.49,756.05~1827.01) G/(cm⁴·s)(<i>P</i>＜0.01), by (12.00±17.27,37.79~28.61) %.The left stroke index, cardiac total power, ejective pressure and left ventricular end diastolic volumewere significantly improved (<i>P</i>＜0.05).The change analysis of each indicator for each patient is shown in the individualized analysis section of this study. <b>Conclusion:</b> Use CPET and continuous functional monitoring we can safely and effectively develop the overall program of individualized exercise in patients with chronic diseases. Long-term intensive manag","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"595-603"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9680484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of total saponins from Trillium tschonoskii Maxim on vascular cognitive impairment and its mechanisms].","authors":"Li-Jun Yang,&nbsp;Gang Wang,&nbsp;Dan Yang,&nbsp;Ren-Ze Duan,&nbsp;Fang-Yu Zhao,&nbsp;Xian-Bing Chen","doi":"10.12047/j.cjap.6355.2022.145","DOIUrl":"https://doi.org/10.12047/j.cjap.6355.2022.145","url":null,"abstract":"<p><p><b>Objective:</b> To investigate neuroprotective effects of total saponins from Trillium tschonoskii Maxim (TST) on vascular cognitive impairment (VCI) rats through inflammatory body of the NOD-like body protein 3 (NLRP3) regulated by endoplasmic reticulum stress (ERS). <b>Methods:</b> SD rats were divided into sham-operated group (SHAM), model group (VCI, bilateral neck arterial ligation (BCCAO) method), TST intervention group (TST, 100 mg/kg), and positive group (donepezil hydrochloride, 0.45 mg/kg ), continuous administration for 4 weeks. The ability of learning and memory was evaluated by the morris water labor. The tissue pathological changes were observed by HE and NISSL staining. Western blot was used to detectendoplasmic reticulum-related proteins GRP78, IRE1, XBP1. Inflammasome-related proteins NLRP3, ASC, Caspase-1, IL-18, IL-1β. <b>Results:</b> Compared with the SHAM group, the escape latency of VCI group rats was prolonged significantly, and the number of times of crossing the platform and the percentage of target quadrant residence time were shortened (<i>P</i>＜0.01); The cells in the hippocampus of VCI rats were damaged, with obvious pyknosis, decreased number of neurons and damage of cell body structure; The endoplasmic reticulum and inflammatory corpuscle-associated proteins were increased in VCI group (<i>P</i>＜0.05 or <i>P</i>＜0.01). Compared with the VCI group, the TST group and the positive group had less time to search for the platform, and the ratio of the times of crossing the platform to the time in the target quadrant was longer (<i>P</i>＜0.05 or <i>P</i>＜0.01). There was no significant difference in the times of crossing the platform between the positive group and VCI group (<i>P</i>＞0.05); The cell damage, nuclear pyknosis and the number of neurons in TST and positive groups were significantly reduced; The endoplasmic reticulum associated proteins and inflammatory body associated proteins in TST group and positive group were decreased to different degrees (<i>P</i>＜0.05 or <i>P</i>＜0.01). <b>Conclusion:</b> TST has neuroprotective effects on VCI rats, and its mechanism may be related to the involvement of ERS in the regulation of NLRP3 inflammatory small bodies.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"797-802"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9624128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of PBMC mitochondrial autophagy on exercise energy consumption and mitochondrial respiratory chain complex enzyme activity in high altitude migrants].","authors":"Hai-Jun Kong,&nbsp;Feng-Hua Wang,&nbsp;Xin-Long Li,&nbsp;Sheng-Qian Gan,&nbsp;Xiao'an Chen","doi":"10.12047/j.cjap.6345.2022.119","DOIUrl":"https://doi.org/10.12047/j.cjap.6345.2022.119","url":null,"abstract":"目的: 探究高原移居中青年男性走、跑状态运动能耗及外周血单个核细胞(PBMC)线粒体自噬标志物变化特征。方法: 招募152名受试者,根据移居高原年限分为高原移居Ⅰ组(HM1)、高原移居Ⅱ组(HM2)、高原移居Ⅲ组(HM3)、世居高原组(Con)。检测受试者3.0 km/h 、4.5 km/h、6.0 km/h及7.5 km/h条件下运动能耗;ELISA检测受试者PBMC线粒体NADH-Q氧化还原酶、琥珀酸-Q氧化还原酶、UQ-细胞色素C氧化还原酶、细胞色素C氧化酶水平;RT-PCR和Western blot检测受试者PBMC线粒体Parkin、PINK3、LC3A、LC3B mRNA及蛋白表达水平。结果: 与Con组比较,HM1、HM2组3 km/h步行能耗显著上升(P＜ 0.05);与HM1组比较,HM2组显著降低(P＜0.05) 。与Con组比较,HM1组4.5 km/h、6 km/h及7.5 km/h走、跑能耗均显著上升(P＜0.05) 。与Con组比较,HM1、HM2和HM3组复合物Ⅰ、复合物Ⅱ及复合物Ⅲ水平均显著降低(P＜0.05) ;与HM1组和HM2组比较,HM3组显著上升(P＜0.05) ;与HM1组比较,HM2组显著上升(P＜0.05) 。与Con组比较,HM1、HM2组复合物Ⅳ水平显著降低(P＜0.05) ;与HM1组和HM2组比较,HM3组显著上升(P＜0.05) 。与Con组比较,HM1、HM2和HM3组Parkin mRNA表达显著上升(P＜0.05) ,HM1、HM2组高于HM3组(P＜0.05) ;与Con组比较,HM1、HM2和HM3组PINK3 mRNA表达显著降低(P＜0.05) ,HM1组显著低于HM2、HM3组(P＜0.05) ;与Con组比较,HM1、HM2和HM3组LC3A mRNA表达显著降低(P＜0.05) ,HM1组和HM2组显著低于HM3组,HM1组显著低于HM2组(P＜0.05) ;与Con组比较,HM1、HM2和HM3组LC3B mRNA和LC3B蛋白表达显著上升(P＜0.05) ,HM1组和HM2组显著高于HM3组,HM1组显著低于HM2组(P＜0.05) 。与Con组比较,HM1、HM2组Parkin蛋白表达显著上升(P＜0.05) ,HM1组、HM2组显著高于HM3组(P＜0.05) ;与Con组比较,HM1、HM2组PINK3和LC3A蛋白表达显著降低(P＜0.05) ,HM1组、HM2组显著低于HM3组(P＜0.05) 。结论: 高原移居者随高原移居时间延长,PBMC线粒体自噬水平逐步下降,PBMC线粒体呼吸酶活性逐渐增殖;高原定量负荷运动能量消耗逐渐降低。.","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"655-659"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9626845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}