Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology最新文献

{"title":"[Effects of <i>Rosa roxburghii</i> on insulin resistance in obese rats and its mechanisms].","authors":"Shuai-Jun Zhang,&nbsp;Jin Zhang,&nbsp;Jin-Ping Guo,&nbsp;Ying-Peng Niu","doi":"10.12047/j.cjap.6328.2022.122","DOIUrl":"https://doi.org/10.12047/j.cjap.6328.2022.122","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the effects of Rosa roxburghii on insulin resistance in obese rats and the regulation of phosphatidylinositol 3-kinase (PI3K)/ protein kinase Bβ(PKB_β/Akt2)/ glucose transporter 4(GLUT4) signaling pathway. <b>Methods:</b> Five-week-old male SD rats were randomly divided into normal control group (NC), model group (M), positive control group (PC), low-dose rosa roxburghii group (LD) and high-dose rosa roxburghii group (HD), with 10 rats in each group. The rats in the NC group were fed with normal diet, while those in the M, PC, LD and HD groups were fed with high-fat diet. From the 13th week, according to the dose standard of 6 ml/kg, rats in the LD group were intragastrically administered with 100 mg/kg Rosa roxburghii Tratt, the HD group were treated with 300 mg/kg Rosa roxburghii Tratt, the PC group were treated with 0.11 g/kg Chiglitazar sodium, and the NC and M groups were intragastrically administered with the same volume of normal saline. The body weight was measured every week until 20 weeks. The rats were sacrificed 24 h after the last experiment. Blood and skeletal muscle were collected. Serum total cholesterol (TC) and triglyceride (TG) contents were detected by colorimetric method, serum superoxide dismutase (SOD) activity was detected by xanthine oxidase method, serum malondialdehyde (MDA) content was detected by thiobarbituric acid method, blood glucose (FBG) value was detected by glucose oxidase method, insulin (FINS) content was detected by ELISA, and PI3K, Akt2, and GLUT4 protein and gene expressions were detected by Western blot and reverse transcription-polymerase chain reaction (RT-PCR). <b>Results:</b> Compared with the NC group, the body weight, serum MDA, TG, TC, FBG, FINS, HOMA-IR levels in the M group were significantly increased (<i>P</i>＜0.01), while SOD activity, PI3K、Akt2、GLUT4 protein and mRNA expression levels were significantly increased(<i>P</i>＜ 0.01). Compared with group M, the body weight, serum MDA, TG, TC, FBG, FINS, and HOMA-IR were decreased significantly in LD group, HD group and PC group (<i>P</i>＜0.05 or <i>P</i>＜0.01), while SOD activity, PI3K, Akt2, GLUT4 protein and mRNA expression levels were increased significantly (<i>P</i>＜0.05 or <i>P</i>＜0.01). <b>Conclusion:</b> <i>Rosa roxburghii</i> can improve insulin resistance in obese rats by antioxidant stress and up-regulating the expressions of PI3K, Akt2, and GLUT4 proteins and genes, which may be related to the PI3K/Akt2/GLUT4 signaling pathway.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"670-675"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9626847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of Mijiandao suppository on intestinal laxation in rats and its mechanisms].","authors":"Xiao-Feng DU,&nbsp;Sheng-Fu Chen,&nbsp;Ting Shang,&nbsp;Zhi-Tao Ni,&nbsp;Yu-Pan Shi,&nbsp;Jin-Hui Wang","doi":"10.12047/j.cjap.6337.2022.141","DOIUrl":"https://doi.org/10.12047/j.cjap.6337.2022.141","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the effects of Mijian Daotong Bowel Suppository (MJDs) on the compound diphenoxylate induced constipation model of male rats and its mechanisms. <b>Methods:</b> Sixty SD male rats were randomly divided into blank group, model group, positive group and MJDs group. The constipation model was established by using compound diphenoxylate gavage. The rats in blank group and model group were treated with saline by enema, the rats in positive group and MJDs group were given Kaisailu and honey decoction laxative suppository by enema, respectively, once a day for 10 days. The body weight, fecal water content, gastric emptying rate (GER) and carbon ink propulsion rate (CIPR) of rats were observed during modeling and administration. The effects of MJDs on the pathological changes of colon tissue in constipation rats were investigated by hematoxylin-eosin (HE) staining. The effect of MJDs on 5-hydroxytryptamine (5-HT) in the colon of constipation rats was investigated by ELISA kit. The effects of MJDs on the expressions of aquaporins 3 (AQP3) and aquaporins 4 (AQP4) in the colon of constipation rats were detected by immunohistochemistry. <b>Results:</b> After 10 days of administration, compared with the blank group, the body weight, fecal water content, carbon ink propulsion rate and colon 5-HT content in the model group were decreased significantly, while the expression levels of AQP3 and AQP4 in the colon were increased significantly (<i>P</i>＜0.05, <i>P</i>＜0.01). Compared with the model group, the fecal water content and colon 5-HT content in the positive group were increased significantly, and the expressions of AQP3 and AQP4 in the colon were decreased significantly. The body weight, fecal water content and colon 5-HT content in the MJDs group were increased significantly, and the expressions of AQP3 and AQP4 was decreased significantly (<i>P</i>＜0.05, <i>P</i>＜0.01). Compared with the positive group, the fecal water content of the MJDs group was decreased significantly, and the expressions of AQP3 and AQP4 in the colon of the MJDs group was decreased significantly (<i>P</i>＜0.05, <i>P</i>＜0.01). Gastric emptying rate was not statistically significant difference between the groups. <b>Conclusion:</b> MJDs has good therapeutic effects on constipation, and its mechanisms may be related to up-regulating the content of 5-HT in the colon and down-regulating the expressions of AQP3 and AQP4 in the colon.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"776-781"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9629799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[An improved method about culture and identification of primary rat bone marrow derived mesenchymal stem cells].","authors":"Hua-Gen Ma,&nbsp;Hai-Qin Liu,&nbsp;Yuan-Yu Tang","doi":"10.12047/j.cjap.6285.2022.134","DOIUrl":"https://doi.org/10.12047/j.cjap.6285.2022.134","url":null,"abstract":"目的: 改良原代大鼠骨髓间充质干细胞(BMSCs)的分离培养方法。方法: 基于“干细胞生态位”原理,采用全骨髓贴壁法结合骨片消化法分离培养原代大鼠BMSCs。通过细胞形态学观察、细胞表面标志物CD分子检测以及定向诱导分化实验对目的细胞进行鉴定。结果: 所培养的细胞形态为细长梭形,其融合后呈典型的“涡旋状”紧密排列,且细胞增殖迅速,原代培养5～6 d即可达到融合状态,按1∶3比例连续传代到第8代时,细胞形态仍基本保持不变;第4代细胞的间充质干细胞表面标志物CD90、CD29、CD73以及造血干细胞表面标志物CD45、CD34、CD11b/c的阳性表达率分别为89.0％、98.3％、64.2％、3.5％、5.6％、 24.7％;定向诱导分化实验表明目的细胞具有良好的成脂、成骨分化潜能。结论: 本研究基于“干细胞生态位”原理,成功建立了一种高效稳定的原代大鼠BMSCs分离培养方法。.","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"735-738"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9624126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of MICT/HIIT on the ultrastructure of myocardium and soleus in rats with high-fat diet and its mechanisms].","authors":"Wei-Dong Wu,&nbsp;Yu Wang,&nbsp;Jian-Xiang Wei","doi":"10.12047/j.cjap.6191.2022.129","DOIUrl":"https://doi.org/10.12047/j.cjap.6191.2022.129","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the effects of moderate intensity continuous training (MICT) and high intensity intermittent training (HIIT) on the ultrastructure of myocardium and soleus in rats with high fat diet, and to explore the mechanisms. <b>Methods:</b> 5-week-old male SD rats were randomly divided into normal diet quiet group (C), high-fat diet quiet group (F), high-fat MICT group (M) and high-fat HIIT group (H), with 8 rats in each group, and the fat content of the high-fat dietary feed was 45%. The M and H groups were given 12 weeks of treadmill running with an incline of 25°. The M group was given continuous exercise with 70%VO₂max intensity, and the H group was given intermittent exercise with 5 min 40%~45%VO₂max and 4 min 95%~99%VO₂max intensity successively. After the intervention, the contents of free fatty acid (FFA), triglyceride (TG), high density lipoprotein cholesterol (HDL) and low density lipoprotein cholesterol (LDL) in serum were detected. Transmission electron microscopy was used to observe the ultrastructure of myocardium and soleus in rats. Western blot was used to detect the protein expressions of AMPK, malonyl-CoA decarboxylase (MCD) and carnitine palmitoyl transterase 1 (CPT-1) in myocardium and soleus. <b>Results:</b> Compared with C group, the body weight, Lee's index, the contents of LDL, TG and FFA in serum were increased, the content of HDL was decreased (<i>P</i>＜0.05), the protein expressions of AMPK and CPT-1 in myocardium and soleus were increased, the protein expression of MCD was decreased (<i>P</i>＜0.05), and the ultrastructure was damaged in group F. Compared with F group, the body weight and Lee's index were decreased, the contents of LDL and FFA in serum were decreased (<i>P</i>＜0.01), the protein expressions of AMPK, MCD and CPT-1 in myocardium were increased, and the protein expressions of AMPK and MCD in soleus were increased (<i>P</i>＜0.05), and the ultrastructural damage was attenuated in M and H groups. Compared with M group, the content of HDL in serum was increased (<i>P</i>＜0.01), the protein expressions of AMPK and MCD in myocardium were increased, and the ultrastructural damage was mild, the protein expression of AMPK in soleus was decreased, the protein expression of MCD in soleus was increased (<i>P</i>＜0.05), and the ultrastructural damage was severe in group H. <b>Conclusion:</b> MICT and HIIT have different effects on the ultrastructure of myocardium and soleus in high-fat diet rats by intervening the protein expression of AMPK, MCD and CPT-1.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"708-713"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9624130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Protective effects of salidroside on vascular endothelial cells in rats with frostbite after chronic hypoxia].","authors":"Hong-Jin Wang,&nbsp;Yi Li,&nbsp;Yan-Ping Feng,&nbsp;Ke-Wei Zhang,&nbsp;Mao-Dong-Zhi Peng","doi":"10.12047/j.cjap.6364.2022.121","DOIUrl":"https://doi.org/10.12047/j.cjap.6364.2022.121","url":null,"abstract":"<p><p><b>Objective:</b> To investigate the protective effects of salidroside on endothelial cells in rats with frostbite after chronic hypoxia. <b>Methods:</b> Healthy male SD rats, randomly divided into 3 groups with 10 rats in each group, which included the sham injury group, the model group, and the model +salidroside group. The rats in each group were placed in a composite low-pressure chamber to simulate a environment with a pressure of 54.1 kpa and a temperature of 23~25°C. The rats were exposed to hypoxia under these conditions for 14 days, during the experimental time the rats in the model+salidroside group were treated with 50 mg/kg salidroside daily. After the rats were removed from the low-pressure chamber, except for the sham injury group, frozen iron sheets were applied tightly to the back of the rats for 30 s, supplemented with low temperature for frostbite modeling. Blood and skin tissues were collected at 12 hours after modeling for testing. The structural changes in tissue and vascular endothelial cells were observed in the frostbite region. Vascular endothelial cell particulate EMP levels were detected. The levels of ICAM-1, sEPCR, vWF, ET-1 and NO secretion were determined. The expression levels of HIF-1α, p-PI3K, p-Akt and VEGF were detected by Western blot. <b>Results:</b> Salidroside could effectively reduce skin collapse in frostbitten areas. It could reduce the injury of frostbitten tissues, and improve the subcutaneous tissue necrosis and inflammatory cell infiltration. The autophagy of vascular endothelial cells was reduced. Compared with the model group (0.250±0.165)%, the expression of EMPs in the model+salidroside group (2.453±0.196)% was increased significantly (<i>P</i>＜0.01). In addition, the contents of NO (2.622±0.219)pg/ml was also significantly higher than that of the model group (1.616±0.152)pg/ml (<i>P</i>＜0.01), and the content of vWF (233.50±13.43)pg/ml was lower than that of the model group (315.60±8.78)pg/ml (<i>P</i>＜0.05). There was no significant difference in the levels of ICAM-1, sEPCR and ET-1. Salidroside significantly decreased the expressions of p-PI3K, p-Akt, VEGF and HIF-1α protein in vascular endothelial cells of rats with frostbite (<i>P</i>＜0.01). <b>Conclusion:</b> Salidroside can reduce endothelial cell damage, reduce endothelial cell autophagy and promote endothelial cell regeneration. Based on the PI3K/Akt pathway, salidroside has a good protective effect on endothelial cells of rats with frostbite after chronic hypoxia.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"664-669"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9626849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Relationship between exhaustion time and blood oxygen saturation of brain and muscle tissues at the moment of exhaustion under high temperature environment].","authors":"Da-Lei Wang,&nbsp;Jian Lang","doi":"10.12047/j.cjap.6365.2022.128","DOIUrl":"https://doi.org/10.12047/j.cjap.6365.2022.128","url":null,"abstract":"目的: 探讨高温环境运动中脑和肌肉组织血氧饱和度的动态变化,以及力竭时间与其力竭即刻时血氧饱和度的关系。方法: 24名男性大学生,在常温、高温环境条件下各进行一次力竭运动,采用近红外光谱技术,对运动及恢复阶段脑组织、肌肉组织血氧饱和度变化情况进行同步观察。监测运动前、后血乳酸水平。计算不同环境条件下力竭时间与力竭即刻脑组织、肌肉组织血氧饱和度的相关性。结果: 高温环境力竭时间极显著低于常温环境(P＜0.01),而力竭即刻血乳酸水平显著高于常温环境(P＜0.05);常温、高温环境条件下,脑组织血氧饱和度随运动持续下降,肌肉组织血氧饱和度呈先上升后下降趋势。力竭即刻,脑组织、肌肉组织血氧饱和度均达最低水平,且高温环境脑组织、肌肉组织血氧饱和度均显著低于常温环境(P＜0.05);常温环境条件下,力竭时间与力竭即刻脑组织、肌肉组织血氧饱和度水平相关系数分别为r= 0.575(P＜0.05)和r= 0.825(P＜0.01)。高温环境条件下,力竭时间与力竭即刻脑组织、肌肉组织血氧饱和度水平相关系数分别为r= 0.632(P＜0.01)和r= 0.539(P＜0.05)。结论: 在高温环境运动中,力竭时间与力竭即刻脑及肌肉组织血氧饱和度呈正相关。.","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"704-707"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9629798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of CUMS on excitatory/inhibitory balance of hippocampal and prefrontal cortex pyramidal neurons in anxiety-like mice<sup />].","authors":"Chuan-An Zhu,&nbsp;Jin-Dong Chen,&nbsp;Hai-Yong Yang,&nbsp;Yu-Ping Xia,&nbsp;Zhi-Yuan Huang","doi":"10.12047/j.cjap.6334.2022.148","DOIUrl":"https://doi.org/10.12047/j.cjap.6334.2022.148","url":null,"abstract":"<p><p><b>Objective:</b> To explore the changes in the excitatory/inhibitory (E/I) balance of pyramidal neurons in prefrontal cortex and hippocampus in mice with anxiety disorder induced by chronic unpredictable mild stress (CUMS). <b>Methods:</b> Twenty-four C57/BL6 male mice were randomly divided into control group (CTRL) and model group (CUMS), with 12 mice in each group. The mice in CUMS group were subjected to 21 days of stress, including restraint for 1 h, reversed day/night cycle for 24 h, forced warm water bath for 5 min, water/food deprivation for 24 h, housing in wet sawdust for 18 h, shaking the cage for 30 min, noise for 1 h, and social stress for 10 min. CTRL group mice were fed normally. Anxiety-related behavioral tests and whole-cell recording tests were performed after modeling. <b>Results:</b> Compared with CTRL group, the time of spent in the central arena of CUMS group was reduced significantly in open field test (<i>P</i>＜0.01), the time and number of entering the open arms were decreased significantly in elevated plus maze test (<i>P</i>＜0.01), and the time of staying in the closed arms was increased significantly in CUMS group (<i>P</i>＜0.01). The sEPSC frequency, capacitance and E/I ratio of dlPFC, mPFC and vCA1 pyramidal neurons of mice in CUMS group were increased significantly (<i>P</i>＜0.01), while sEPSC amplitude, sIPSC frequency, amplitude and capacitance were not significantly changed (<i>P</i>＞0.05). The frequency, amplitude, capacitance and E/I ratio of sEPSC and sIPSC of dCA1 pyramidal neurons were not significantly changed (<i>P</i>＞0.05). <b>Conclusion:</b> The anxiety-like behavior of CUMS-induced mice may be the result of the participation of multiple brain regions, which is mainly related to the increase of the excitability of pyramidal neurons in dlPFC, mPFC and vCA1 brain regions, but seems to have little relationship with dCA1 brain regions.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"814-819"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9624123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of endurance exercise on glucose uptake of skeletal muscle in mice and regulation mechanism of interleukin 15].","authors":"Zhe Wang,&nbsp;Qian Wang,&nbsp;Hua-Duo Wu,&nbsp;Yong-Qiang Hu,&nbsp;Zi-Fu Peng,&nbsp;Xiang-Ying Guo,&nbsp;Ning Jiang","doi":"10.12047/j.cjap.6360.2022.126","DOIUrl":"https://doi.org/10.12047/j.cjap.6360.2022.126","url":null,"abstract":"目的: 探讨耐力运动对小鼠骨骼肌葡萄糖摄取能力的影响及白细胞介素(IL-15)的作用机制。方法: 将32只C57BL/6J小鼠随机分为安静对照组(Control, C)、耐力训练组(Exercise, E)、PBS注射组(PBS injected, PI)、IL-15注射组(IL-15 injected, II)。每组8只,C组小鼠正常饲养8周,E组小鼠进行8周中等强度耐力训练,II组小鼠一次性腹腔注射300 μl IL-15复合剂溶液(1.5 μg IL-15和7 μg IL-15Rα),PI组一次性腹腔注射与II组等量的PBS。其中C组和E组在饲养第7周时,PI组和II组在一次性注射后,各组小鼠禁食12 h,随后进行葡萄糖耐量试验(IPGTT)检测并记录血糖水平。采用Western blot检测小鼠骨骼肌GLUT4、IL-15、AKT、pAKT、AMPK、pAMPK、AS160、pAS160的蛋白表达水平。结果: 与C组相比,E组小鼠骨骼肌IL-15、GLUT4、pAMPK、pAKT、pAS160蛋白表达明显升高(P＜0.05)。与PI组相比,II组小鼠骨骼肌IL-15、GLUT4、pAMPK、pAS160蛋白表达明显升高(P＜0.05)。结论: 8周耐力运动和腹腔注射IL-15复合剂均可提升小鼠骨骼肌葡萄糖的摄取能力,但单独增加IL-15可能是通过激活AMPK/AS160/GLUT4通路提升对于葡萄糖的转运能力,这一过程不依赖AKT/AS160/GLUT4通路的激活。.","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"696-699"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9624125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Intervention effects of estradiol on myocardial ischemia- reperfusion injury of rat and its mechanisms].","authors":"Jing-Ru Feng,&nbsp;Hai-Yang Zhang,&nbsp;He Shi,&nbsp;Teng-Fei Wang,&nbsp;Zi-Jian Wang,&nbsp;Guang-Hui Cheng,&nbsp;Sheng-Li Bi","doi":"10.12047/j.cjap.6362.2022.116","DOIUrl":"https://doi.org/10.12047/j.cjap.6362.2022.116","url":null,"abstract":"<p><p><b>Objective:</b> To study the effects of estradiol (E2) on alleviating myocardial ischemia/reperfusion(I/R) injury through estrogen receptorβ(ERβ) mediated extracellular regulated protein kinases(ERK) pathway activation. <b>Methods:</b> Eighty-four adult female SD rats were ovariectomized and randomly divided into control group, NC siRNA adeno-associated virus (AAV) group received sham operation, the myocardial I/R injury model was prepared by ligation of the left anterior descending coronary artery in I/R group, E2+I/R group, NC siRNA AAV+I/R group, NC siRNA AAV+E2+I/R group and ERβ-siRNA AAV+E2+I/R group. E2+I/R group, NC siRNA AAV+E2+I/R group and ERβ-siRNA AAV+E2+I/R group were treated with E2 0.8 mg/kg by gavage for 60 days before modeling. NC siRNA AAV+I/R group, NC siRNA AAV+E2+I/R group, and ERβ-siRNA AAV+E2+I/R group were treated with AAV by caudal vein injection 24 h before modeling. After 120 min of reperfusion, the contents of serum lactate dehydrogenase (LDH), phosphocreatine kinase (CK), phosphocreatine kinase isoenzyme (CK-MB), myocardial infarction area and the expressions of ERβ, p-ERK, the contents of tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1 β), malondialdehyde (MDA) and total antioxidant capacity (T-AOC) in myocardium were measured. <b>Results:</b> The contents of serum LDH, CK, CK-MB, myocardial infarction area and the contents of TNF-α, IL-1 β, MDA in myocardium of I/R group were higher than those of the control group, the expression levels of ERβ and p-ERK and the content of T-AOC were lower than those in the control group (<i>P</i>＜0.05). The contents of serum LDH, CK and CK-MB, myocardial infarction area and the contents of TNF-α, IL-1 β and MDA in myocardium of E2+I/R group were lower than those of the I/R group, the expression levels of ERβ and p-ERK and the content of T-AOC were higher than those of the I/R group(<i>P</i>＜0.05). After knockdown ERβ by caudal vein injection of ERβ-siRNA AAV, the contents of serum LDH, CK and CK-MB, myocardial infarction area and the contents of TNF-α, IL-1 β and MDA in myocardium of ERβ-siRNA AAV+E2+I/R group were higher than those of NC-siRNA AAV+E2+I/R, the expression levels of ERβ and p-ERK and the content of T-AOC were lower than those of NC-siRNA AAV+E2+I/R(<i>P</i>＜0.05). <b>Conclusion:</b> E2 has protective effects on myocardial I / R injury in ovariectomized rats, which are related to the promotion of ERβ mediating the activation of ERK pathway, reducing inflammatory and oxidative stress responses.</p>","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 6","pages":"638-643"},"PeriodicalIF":0.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9680486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of hypoxic training on gene expressions of p53 and its regulated mitochondrial aerobic metabolic signaling pathway in skeletal muscle].","authors":"Jie Li,&nbsp;Li-Li Zhao","doi":"10.12047/j.cjap.6314.2022.105","DOIUrl":"https://doi.org/10.12047/j.cjap.6314.2022.105","url":null,"abstract":"目的: 探讨低氧训练对大鼠骨骼肌p53及其调控的线粒体有氧代谢信号通路基因表达的影响,以及对线粒体有氧氧化供能能力的影响。方法: 30只雄性Wistar大鼠随机分为3组(n=10),低住低练组(LoLo)、高住高练组(HiHi)和高住高练低训组(HiHiLo)。以当地海拔1 500 m为常氧环境,模拟海拔3 500 m为低氧环境,各组大鼠按训练方案训练5周后,取股四头肌行匀浆及提取线粒体。Real-time PCR检测p53、细胞色素c氧化酶合成2(SCO2),细胞色素c氧化酶亚基Ⅰ(COXⅠ)和谷氨酰胺酶2(GLS2) mRNA表达,Westem blot检测 p53、SCO2、COXⅠ和GLS2蛋白表达;ELISA测定α-酮戊二酸脱氢酶(α-KGDHC),细胞色素c氧化酶(COX)及ATP合酶(ATP synthase)活性。结果: ①与LoLo 组比较,HiHi和HiHiLo组p53 mRNA水平显著升高(P＜ 0.01),HiHiLo组p53蛋白表达水平显著下降(P＜0.01);HiHi和HiHiLo组SCO2 mRNA水平和蛋白表达水平均显著升高(P＜ 0.01);HiHiLo组COXⅠmRNA水平显著升高(P＜0.01),HiHi组显著降低(P＜0.01),HiHi组COXⅠ蛋白表达水平显著升高(P＜ 0.05);HiHi和HiHiLo组GLS2 mRNA水平均显著升高(P＜0.05,P＜0.01),HiHiLo组GLS2蛋白表达水平显著下降(P＜0.01)。②与LoLo组比较,HiHi组ɑ-KGDHC和COX活性均显著降低(P＜0.01,P＜0.05),HiHiLo组均显著升高(P＜0.01);HiHi和HiHiLo组ATP synthase总活性和特异性活性均显著升高(P＜0.01)。结论: 高住高练低训上调大鼠骨骼肌p53及其调控的线粒体有氧代谢信号通路相关因子基因转录水平,提高骨骼肌线粒体有氧氧化供能能力。.","PeriodicalId":23985,"journal":{"name":"Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology","volume":"38 5","pages":"564-568"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9416161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}