Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases最新文献

{"title":"[Evaluation of Measures and Achievements of Malaria Control in Tengchong City, Yunnan Province during 2010-2015].","authors":"Jia-zhi Wang,&nbsp;Shou-qin Yin,&nbsp;Sheng-guo Li,&nbsp;Xi-shang Li,&nbsp;Wen-bin Cai,&nbsp;Jun Feng","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the measures and achievements of malaria control in Tengchong City during 2010-2015.</p><p><strong>Methods: </strong>The malaria control information on epidemiology, foci disposal, blood detection of febrile patients, and medical treatment during 2010-2015 in Tengchong City was collected and analyzed using Microsoft Excel 2010.</p><p><strong>Results: </strong>In 2010-2015, 1 654 malaria cases were reported in Tengchong City, including 18 indigenous cases, 22 domestically mobile cases, and 1 614 imported cases from abroad, of whom 1 584 cases （98.1%） were imported from Myanmar. Most of the cases were vivax malaria（76.2%, 1 261/1 654）. No indigenous malaria cases were reported from 2013 to 2015. Blood test was conducted for 80 655 febrile patients, with a positive detection rate of 2.1%（1 654/80 655）. The positive detection rate was highest in 2010 （2.8%, 700/24 861）, lowest in 2011（1.4%, 341/23 623）, and decreased from 2012 to 2015. In addition, 1 654 cases were directly reported through online system. The 24-h case report rate during 2013-2015 was 100%. A total of 1 191 cases were investigated. The 3-day case investigation rate during 2013-2015 was 100%. A total of 1 351 endemic foci were investigated. The 7-day foci disposal rate during 2014-2015 was 100%.</p><p><strong>Conclusion: </strong>No indigenous transmission has been reported for three years in Tengchong City. However, the imported malaria remains an important problem.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 5","pages":"435-8"},"PeriodicalIF":0.0,"publicationDate":"2016-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36412504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Digital Description and Identification of 11 Kinds of Principal Parasite Eggs].","authors":"Hai-mo Shen,&nbsp;Lin Ai,&nbsp;Yu-chun Cai,&nbsp;Yan Lu,&nbsp;Shao-hong Chen","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To facilitate the identification of parasite eggs using computer technology, establish the automation-based applications, and propose an algorithm for egg classification.</p><p><strong>Methods: </strong>Eggs of 11 parasites, Clonorchis sinensis, Taenia solium, Enterobius vermicularis, Ascaris lumbricoides, Trichuris trichiura, Spirometra mansoni, Diphyllobothrium latum, Ancylostoma duodenale, Schistosoma japonicum, Paragonimus westermani and Fasciolopsis buski, were selected and divided into two groups, the training group and the testing group, and were microphotographed. The eigenvalue was extracted using the VC++-based method. The eigenvalue database was constructed, and the training data set was tested with a variety of classification algorithms. The classifier was constructed using algorithm with the highest efficiency and an identification method was established by multi-feature fusion.</p><p><strong>Results: </strong>After removal of images with invalid values, the training group received 19 844 egg images, and the testing group, 3 721 images. Based on the 14 eigenvalues, there were significant differences in the size and color among the eggs of 11 parasite species. For example, the length, width, area and brightness of the smallest parasite egg of Clonorchis sinensis were 292.24 μm, 192.64 μm, 43 416.61 μm2 and 53.84, respectively, while those of the largest parasite egg of Fasciolopsis buski were 945.31 μm, 610.88 μm, 536 002.60 μm2 and 100.54, respectively. When using dynamic weights to construct the classifier, the discrimination rate on the training data set was 88.89%（17 641/19 844）, and that on the verification data set was 91.83%（3 004/3 271）, with an average modeling time of 0.01 s.</p><p><strong>Conclusion: </strong>The algorithm for egg classification has been established, which pravides a basis for further study on its feasibility.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 5","pages":"424-9"},"PeriodicalIF":0.0,"publicationDate":"2016-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36415149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Exploration and Application of Discussion-based Teaching Method in Teaching of Medical Parasitology].","authors":"Ju-xiang Su,&nbsp;Lian-shun Cai,&nbsp;Guang Chen,&nbsp;Yue Dai,&nbsp;Hui-ming Zhang,&nbsp;Zi-lin Gong,&nbsp;Shi-wei Che","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In this study, students majoring in Clinical Medicine were enrolled to explore the effect of discussion-based teaching method in the teaching of medical parasitology. One hundred and fifty-six students (with an entry year of 2011) in classes 1-3 received the discussion-based teaching while 153 students in classes 4-6 received traditional teaching. The effect of teaching was evaluated in terms of final examination score and questionnaire, and compared between the groups. The final examination score of students receiving the discussion-based teaching （86.1±6.6） was significantly higher than those receiving the traditional teaching（74.2±8.3）（P<0.05）. The discussion-based teaching method was graded as “excellent” by 89.1%（136/156）of the students, and was considered to be superior to the traditional teaching by 96.8%（151/156）of the students. The results indicate that the discussion-based teaching method can enhance interactions between participants, change the ways of thinking, and provide inspirations for learning and exploration.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 5","pages":"473-5"},"PeriodicalIF":0.0,"publicationDate":"2016-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36416033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Serological Analysis of Antibodies against Cysticercus cellulosae and Toxoplasma gondii in 122 Patients with Meningitis Encephalitis Syndrome].","authors":"Lei Sun,&nbsp;Yan-juan Wang,&nbsp;Yuan Hu,&nbsp;Sheng-kui Cao,&nbsp;Yu-juan Shen,&nbsp;Jian-ping Cao","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To examine the IgG and IgM antibodies for parasites Cysticercus cellulosae and Toxoplasma gondii in 122 patients with meningitis encephalitis syndrome, and provide basis for clinical diagnosis of the meningitis encephalitis syndrome.</p><p><strong>Methods: </strong>The sera were collected from patients with meningitis encephalitis syndrome in Shanghai Jiaotong University Affiliated Sixth People’s Hospital, People’s Hospital of Danyang City, and Jiangsu University Affiliated Hospital from August, 2014 to December, 2015. Serum IgG and IgM antibodies for cysticercus and T. gondii were examined using antibody test kits. The antibody positive rate was calculated and its distribution was analyzed by gender, season, age and occupation.</p><p><strong>Results: </strong>A total of 122 patients with meningitis encephalitis syndrome were included. Seventeen and 22 patients of them were positive for IgG （13.9%, 17/122） and IgM（18.0%, 22/122） against cysticercus, respectively, while 29 and 8 cases were positive for IgG （23.8%, 29/122） and IgM （6.6%, 8/122） against T. gondii. The positive rate of cysticercus and T. gondii in males was 30.6%（22/72） and 31.9%（23/72） respectively, while that in females was 26.0%（13/50） and 24.0% （12/50）. The positive rate of IgM against cysticercus was 12.0%（3/25）, 27.0%（17/63）, 6.9% （2/29）, and 0（0/5） from spring to winter, highest within 13-25 years（45.0%, 9/20） among age groups, and highest in workers（7/14） among various occupations. The positive rate of IgM against T. gondii was 4.0%（1/25）, 11.1% （7/63）, 0（0/29）, and 0（0/5） from spring to winter, highest in ages >65 years（44.0%, 11/25）, and highest in patients with other occupations（4/10）. There was no statistically significant difference in the positive rate between males and females, and among different seasons, ages and occupations.</p><p><strong>Conclusion: </strong>The positive rate of antibodies against cysticercus and T. gondii is high in the patients included, suggesting that a serological test for parasite infection might be performed during clinical diagnosis of meningitis encephalitis syndrome.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 5","pages":"414-7"},"PeriodicalIF":0.0,"publicationDate":"2016-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36416269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Antigenic Characterization of Immunodominant Epitopes of Alpha-8 giardin in Giardia lamblia].","authors":"Chao-jun Wei,&nbsp;Yan-juan Jia,&nbsp;Hui Xu,&nbsp;Ling Wu,&nbsp;Si-qi Lu,&nbsp;Yong-hong Li","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To identify the dominant epitopes of α-8 giardin in Giardia lamblia, and analyze their immunoreactivity.</p><p><strong>Methods: </strong>Three dominant epitopes G1（7-17 aa）, G2（30-40 aa） and G3（296-306 aa） were predicted for α-8 giardin by bioinformatic analysis using DNAstar, Biosun and CLUSTAL W softwares. Each epitope was conjugated to keyhole limpethemocyanin (KLH) and then used to immunize female Chinchilla rabbits via multi-point（8-10 points） subcutaneous injections on the back（0.5 mg conjugate/rabbit, 100 μl at each point）. Then the rabbits were boosted（0.2 mg conjugate/rabbit） on days 14, 21 and 28 after the first immunization. Blood was collected prior to each immunization, and carotid artery blood（50 ml） was collected on day 7 after the last immunization. The antibody titer for anti-KLH-G1, anti-KLH-G2 and anti-KLH-G3 sera was determined with indirect ELISA. The reactivity of the three anti-sera with recominant giardin (rGiardin) was analyzed with Western blotting.</p><p><strong>Results: </strong>The concentration of the purified conjugates KLH-G1, KLH-G2, and KLH-G3 was 0.66, 0.95 and 0.25 mg/ml， respectively. ELISA showed that the antibody titer for anti-KLH-G1, anti-KLH-G2 and anti-KLH-G3 sera was 1∶12800, 1∶51200, 1∶51200, respectively. SDS-PAGE revealed a specific band at M(r) 36 000 for the three anti-sera. Western blotting showed that the three anti-sera all specifically recognized rGiardin.</p><p><strong>Conclusion: </strong>Immunization with the KLH-G1, KLH-G2, and KLH-G3 conjugates induced production of specific IgG antibody in rabbits, which can recognize rGiardin.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 5","pages":"389-93"},"PeriodicalIF":0.0,"publicationDate":"2016-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36417302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Establishment of LAMP Detection for Toxoplasma gondii Based on the 529 bp Repeat Sequence].","authors":"Qian-ming Xu,&nbsp;Heng-qing Yuan,&nbsp;Chang-cheng Zhao,&nbsp;Fen Fang,&nbsp;Zhe Liu,&nbsp;Hong-rui Ning,&nbsp;Da-li Shi,&nbsp;Yu Li,&nbsp;Hai-yang Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To establish the loop mediated isothermal amplification（LAMP） technique for determining Toxoplama gondii.</p><p><strong>Methods: </strong>The primers for LAMP of the conserved 529 bp sequence was designed by the Primer Explorer 4.0 software. The LAMP reaction was made on the constructed pMD-19T-529 bp recombinant plasmid as a template, and was optimized in loop primer, concentrations of betaine and MgSO4, and reaction temperature. The optimized LAMP and PCR were performed in ultra-pure water, on pig genome, Cryptosporidium parvum genome, Isospora suis genome, and pMD-19T-529 bp, respectively, to test the sensitivity of LAMP. The pMD-19T-529 bp was serially diluted to 109-100 copies/ml to test the specificity of LAMP.</p><p><strong>Results: </strong>LAMP using the designed primers amplified the 529 bp fragment of T. gondii. The optimized LAMP system had a total reaction volume of 25 μl, with the optimal concentrations of betaine and MgSO4 being 0.4 mol/L and 6 mmol/L, respectively. The amplification efficiency of 30 min reaction in the presence of loop primer was comparable to that of 60 min reaction without loop primer, which indicated that addition of loop primer shortened the reaction time by 30 min. The optimal reaction condition was 63 ℃ 30 min, 80 ℃ 3 min. The established LAMP method specifically amplifed the 529 bp fragment of T. gondii, and its efficiency was 10 times of PCR（detection threshold 103 copies/ml vs. 104 copies/ml）.</p><p><strong>Conclusion: </strong>The established LAMP for detecting Toxoplama gondii 529 bp repeat sequence shows high specificity and sensitivity.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 5","pages":"444-7"},"PeriodicalIF":0.0,"publicationDate":"2016-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36416527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Screening and Applications of qPCR Primers for apomucin Gene of Echinococcus multilocularis].","authors":"Meng Su,&nbsp;Xiao-la Guo,&nbsp;Jing Yang,&nbsp;Zhong-wei Shao,&nbsp;Jun-tao Ding,&nbsp;Hai-tao Xiang,&nbsp;Xue-nong Luo,&nbsp;Ya-dong Zheng,&nbsp;Xiao-lin Sun","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To screen for the optimal qPCR primers for Echinococcus multilocularis apomucin gene (Em-apo) and analyze Em-apo expression.</p><p><strong>Methods: </strong>Primers were designed based on 4 Em-apo sequences from GeneDB. Primer specificity and PCR efficiency were determined, based on which the optimal primer pairs were selected. Alterations of Em-apo expression in 1 000 E. multilocularis protoscoleces treated with albendazole（5 μg/ml） and insulin（100 ng/ml） were separately assessed using the selected primers. DMSO used in albendazole dilution and in PBS insulin dilution were used as the control.</p><p><strong>Results: </strong>Specific primers for Em-apo-1, Em-apo-2/3, Em-apo-4 and actin were selected. qPCR melting curves revealed a single peak for each primer pair and an amplification efficiency from 95% to 101%. The qPCR showed increased expression of Em-apo-1（1.51±0.27）, Em-apo-2/3 （1.39±0.30） and Em-apo-4（1.14±0.18） after albendazole treatment in comparison to the DMSO control（1.00）（P>0.05 among the three genes）; and an unaltered Em-apo-1 expression, slightly decreased Em-apo-4 expression, and significantly decreased Em-apo-2/3 expression（0.73±0.09） after insulin treatment in comparison to the PBS control (P>0.05 among the three genes）.</p><p><strong>Conclusion: </strong>The selected specific primers for Em-apo genes can be used to analyze the gene expression by qPCR. Treatment with albendazole and insulin show certain effects on the expression of Em-apo genes in E. multilocularis protoscoleces.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 5","pages":"394-8"},"PeriodicalIF":0.0,"publicationDate":"2016-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36417298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Polymorphism Analysis of Plasmodium falciparum K13 Gene Kelch Domain Associated with Resistance to Artemisinin in Yunnan Province].","authors":"Ai-ming Sun,&nbsp;Ying Dong,&nbsp;Meng-ni Chen,&nbsp;Yan-chun Xu,&nbsp;Yan Deng,&nbsp;Xiang-hua Mao,&nbsp;Jian Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the polymorphism of Plasmodium falciparum K13 gene kelch domain region and provide basis for understanding the artemisinin resistance of falciparum malaria in Yunnan Province.</p><p><strong>Methods: </strong>The filter blood samples and relative information of falciparum malaria cases were collected in 16 prefectures of Yunnan Province from January 2013 to December 2015. The source of infection was determined by epidemiological investigation and the place of case discovery was confirmed according to the China Information System for Disease Control and Prevention Epidemic Registration. The K13 gene kelch domain region was amplified by nested PCR, sequenced, and blasted against the reference strain 3D7（PF3D7_1343700）. The K13 gene kelch domain region polymorphism was analyzed with Mega 5.04. The variable sites and genetic distance between sequences were analyzed. The constituent ratio of amino acid mutation sites was calculated and analyzed with χ2 test.</p><p><strong>Results: </strong>A total of 202 blood samples were collected from 2013 to 2015, comprising 190 from imported cases, 12 from local cases in Yunnan Province. The constitutent ratio of infection cases were 30.7% （62/202）, 34.2% （69/202） and 35.1% （71/202） respectively, increased year by year. The K13 gene kelch domain was successfully amplified from 192 samples and 190 were successfully sequenced, detecting missense mutation of K13 gene in 66 samples, the mutation rate was 34.7% （66/190）. The detection rate of K13 gene mutation was 40.9% （27/66）, 37.9% （25/66） and 21.2% （14/66） respectively, decreased year by year. In this study, ten types of mutations were detected, which were F446I, A578S, N458Y, P574L, A676D, G449A, C469Y, V494I, E556D and S16L. The highest mutation rate occurred in F446I which was 72.7% （48/66）. The proportion of F446I mutation type was 58.3% （28/48） in an age-range of 18-56 years, 70.8% （34/48） in farmers, and 91.7% （44/48） in patients with infection source in Southeast Asia, all significantly higher than that of other groups with the same characteristics （41.7%, 20/48; 29.2%, 14/48; and 8.3%, 4/48, respectively）（χ2=4.633, 5.556 and 5.152, both P＜0.05）. There was a 248 bp homologous sequence in the 190 sequences, composed of 235 conservative sites （94.8%）, 13 variable sites （5.2%）, 5 parsim-info sites （2.0%）, and 8 singleton sites （3.2%）. The genetic distance among the 190 sequences ranged 0.000-0.036, with an average of 0.001±0.001.</p><p><strong>Conclusion: </strong>There are 10 types of mutations in the K13 kelch domain in Yunnan Province, the predominant mutation type was F446I.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 4","pages":"339-45"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36432245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The Impact of the Malaria National Strategy Application Program of the Global Fund on Malaria Elimination in Henan Province].","authors":"Yan Deng,&nbsp;Wei-qi Chen,&nbsp;Ya-lan Zhang,&nbsp;Dan Qian,&nbsp;Ying Liu,&nbsp;Rui-min Zhou,&nbsp;Hong-wei Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To analyze the impact of the national strategy application （NSA） program of the global fund on malaria elimination in Henan Province.</p><p><strong>Methods: </strong>The internal control system on project, fund and supplies was developed to ensure the normalized implementation of NSA program in Henan Province. The multi-channel communication platform including telephone, e-mail and so on was established for data’s progressively reporting, review and summary. The training, supervision and assessment were implemented to ensure that the data was real and correct. The data derived from the NSA program and other project involved with malaria control in the Province from 2009 to 2012 was collected and compared. Changes on human resources, financial input, supplies purchasing and the main malaria control measures before and after the implementation of the program were analyzed.</p><p><strong>Results: </strong>A total of 29.72 million yuan financial support which accounted for more than half of the total financial input on malaria control at the same time in the Province was provided by the NSA program. A total of 3 966 microscopists and 61 415 health workers from primary health care received skill training on malaria dectection and control. After implementation of the program, there was an increase in the diagnosis rate by blood test from 2009 （45.67%, 738/1 616） to 2012 （99.36%, 155/156） （χ2=161.91. P＜0.01）. The malaria incidence showing a step-wise decline from 2009 to 2012 which were 1.71/100 000, 0.94/100 000, 0.34/100 000 and 0.17/100 000 respectively, with an increase in imported cases（χ2=1 310.70, P＜0.01） and no report of indigenous malaria cases in 2012, for the first time. The reported cases in counties of the NSA program during 2009-2012 constituted 98.08%（1 585/1 616）, 92.75% （831/896）, 80.25% （252/314） and 67.31% （105/156） of all malaria cases reported in that year in the Province （χ2=300.38, P＜0.01）. The awareness rates on malaria prevention and treatment among residents and students were 94.65% （25 246/26 673） and 93.73% （16 328/17 421） in 2012, both significantly higher than the baseline in 2009 （63.88%, 5 755/9 009; 53.39%, 3 170/5 937） （χ2=5 594.01, P＜0.01; χ2=5 221.53, P＜0.01）.</p><p><strong>Conclusion: </strong>The implementation of the NSA program has motivated the elimination of malaria in Henan Province.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 4","pages":"346-9"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36432246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Survey on Toxoplasma gondii Infection in Depressed Patients and Genotype Identification in Guizhou Province].","authors":"Cai-yan Zou,&nbsp;Jun He,&nbsp;Ji Yin,&nbsp;Bin Bi,&nbsp;Ying Qin,&nbsp;Feng-tao Peng,&nbsp;Yu Xia,&nbsp;Meng-yuan Tian,&nbsp;Yong-nian Li","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To survey on Toxoplasma gondii infection in depressed patients in Guizhou Province and identify the genotype of T. gondii.</p><p><strong>Methods: </strong>Enzyme-linked immunosorbent assay (ELISA) was applied to detect the T. gondii-specific antibodies IgG, IgM and circulating antigens (CAg) of T. gondii in 141 patients and 150 healthy subjects. The specific repeated DNA fragment (529 bp) of T. gondii was amplified by PCR. The genotype of T. gondii was determined by multiplex multilocus nested polymerase chain reaction-restriction fragment length polymorphism (Mn-PCR-RFLP).</p><p><strong>Results: </strong>ELISA showed that the positive rate of anti-T. gondii antibody in depressed patients and healthy subjects was 21.3%（30/141） and 7.3%（11/150）, respectively. The positive rate of IgG in depressed patients was 18.4% （26/141）, significantly higher than that in healthy subjects （7.3%, 11/150）（P<0.05）. The positive rate of IgM and CAg in depressed patients was both 1.4% （2/141）, while these were not found in healthy subjects. PCR revealed one patient positive for T. gondii, whose genotype was further identified to be Toxo DB #9（Chinese 1 type） by Mn-PCR-RFLP.</p><p><strong>Conclusion: </strong>The positive rate of T. gondii is higher in depressed patients than in the healthy subjects in Guizhou Province. The genotype of T. gondii detected in one depressed patient is the Chinese 1 type.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 4","pages":"355-60"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36432995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}