Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases最新文献

{"title":"[Prokaryotic Expression, Purification and Immunological Characterization of Micronemal Protein 16 of Toxoplama gondii].","authors":"Jin Li,&nbsp;Yong Cui,&nbsp;Kun Yin,&nbsp;Gong-zhen Liu,&nbsp;Ting Xiao,&nbsp;Chao Xu,&nbsp;Qing-kuan Wei,&nbsp;Bing-cheng Huang,&nbsp;Hui Sun","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To prokaryotically express three gene fragments of micronemal protein 16 （TgMIC16） of Toxoplasma gondii, and analyze the immunoreactivity of the three recombinant protein products.</p><p><strong>Methods: </strong>Primers were designed for three fragments of TgMIC16 gene which encode proteins within the functional domain. Reverse-transcription PCR was used to generate cDNA from RNA, and the three fragments were amplified on the cDNA by PCR using the designed primers. The PCR products were double-digested, inserted into the pET-32a（+） plasmid, and transformed into Escherichia coli TOP10 cells. Plasmids extracted from positive clones were confirmed by BamHⅠ/HindⅢ double digestion and sequencing, and further transformed into E. coli Rosetta cells. Protein expression was induced by IPTG, and confirmed by SDS-PAGE. The expressed recombinant proteins were purified with Ni-NTA affinity chromatography and their immunoreactivity analyzed with Western blotting.</p><p><strong>Results: </strong>The amplified three fragments were 1 806, 1 290 and 855 bp in size. Double digestion and sequencing results confirmed the successful construction of the three recombinant plasmids. SDS-PAGE analysis showed successful expression of the three recombinant proteins (M(r) 88 000, 68 000 and 52 000, respectively）, in the form of inclusion bodies. Western blotting showed that the three purified recombinant proteins reacted with His monoclonal antibody and rabbit anti-T. gondii antibody.</p><p><strong>Conclusion: </strong>The three fragments within the functional domain of TgMIC16 are successfully expressed in prokaryotic expression system and show immunoreactivity.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 3","pages":"198-202"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36416402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Immunotherapeutic Effect of Dermatophagoides pteronyssinus Group 1 Allergen T Cell Epitope Peptide Against Allergic Asthma in Mice].","authors":"Chao-pin Li,&nbsp;Bei-bei Zhao,&nbsp;Xiao-dong Zhan","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To study the specific immunotherapeutic effect of Dermatophagoides pteronyssinus group 1 major allergen T-cell fusion epitope peptide vaccine TAT-IhC-DPTCE against allergic asthma.</p><p><strong>Methods: </strong>One hundred and twenty SPF-grade BALB/c mice were randomized into PBS group (group A), asthma group （group B）, and immune treatment groups respectively receiving intraperitoneal (i.p.) injections of ProDer p 1 allergen (group C), DPTCE （group D）, TAT-DPTCE (group E) or TAT-IhC-DPTCE (group F) （n=20 in each group）. In detail, PBS （group A） or allergen extract derived from Dermatophagoides pteronyssinus （groups B-F, 10 μg） was intraperitoneally injected on days 0, 7 and 14, and was continued by aerosol inhalation from day 21 for 7 consecutive days （0.5 μg/ml, once/day, 30 min each time）. The mice in groups C-F received i.p. injections of 100 μg/ml ProDer p 1, DPTCE, TAT-DPTCE and TAT-IhC-DPTCE respectively 30 min prior to inhalation challenge on days 25-27 as a specific immunotherapy, while those in groups A and B received 200 μl PBS. Twenty-four hours after the last inhalation challenge, all the mice were sacrificed. The lung histopathological changes were examined by HE staining. The levels of IFN-γ, IL-13, IL-10 and TGF-β in the bronchoalveolar lavage fluid (BALF) was determined with ELISA, and eosinophils in the BALF were counted (n=20 mice in each group). The serum level of IgE, IgG1 and IgG2a in orbital blood was determined by ELISA（n=5 mice in each group）.</p><p><strong>Results: </strong>HE staining revealed increased BALF eosinophils and decreased pulmonary inflammation in group F compared with group B. The IFN-γ level in group F ［（298.75±26.09） pg/ml］ was significantly higher than those in groups B［（158.71±20.89） pg/ml］, C［（210.38±18.92） pg/ml］, D ［（229.44±13.00） pg/ml］ and E［（233.24±20.39） pg/ml］ （all P<0.01）. Similar results were also found for IL-10 and TGF-β, while the IL-13 levels in groups C ［（47.35±4.71） pg/ml］, D ［（41.90±4.28） pg/ml］, E［（41.05±6.50） pg/ml］ and F［（18.53±5.67） pg/ml］ were all significantly lower than that in group B ［（66.68±6.63） pg/ml］（all P<0.01）. The number of BALF eosinophils in group B ［5.65±0.91］×105/ml］ was significantly higher than that in group A ［（0.45±0.39）×105/ml］ （P<0.01）, while the BALF eosinophils in groups C ［（4.00±0.59）×105/ml］, D ［（3.39±0.63）×105/ml］, E ［（3.24±0.69）×105/ml］ and F ［（1.42±0.49）×105/ml］ decreased after immune treatment (all P<0.01). ELISA results showed that the serum IgE level in group F ［（5.26±1.72） ng/ml］ was significantly lower than those in group B ［（32.81±2.98） ng/ml］ and the other 3 treatment groups [group C, （20.06±3.17） ng/ml; D, （17.06±3.18） ng/ml; E, （16.23±3.61） ng/ml］. Similar results were also obtained for IgG1. In contrast, the serum IgG2a level in group F［（43.10±1.34） ng/ml］ was significantly higher than those in group B［（12.61±1.87） ng/ml］ and the other 3 treatment groups ［group C, （23.37±2.67） ng/ml; D, （25.60±2.10） ng/ml; E, （2","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 3","pages":"214-9"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36416855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[In vitro Killing Effect of Eucalyptus robusta Leaves Extract on Echinococcus granulosus Protoscolices].","authors":"Yu-ning Zhao,&nbsp;Hao-bing Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effect of Eucalyptus robusta leaves extract against Echinococcus granulosus protoscolices in vitro.</p><p><strong>Methods: </strong>Mature leaves of Eucalyptus robusta were collected on 24th day in each month from January to December 2012, and air-dried in the room. Ultrasonic extraction of the leaves was done with 4 solvents with different polarity, petroleum ether, dichloromethane, ethyl acetate and anhydrous ethanol. Protoscolices were incubated with the extract at various concentrations for 72 h, and mortality and median lethal dose（LC50） was calculated.</p><p><strong>Results: </strong>The extracts were different in characteristics and yield. The petroleum ether extract was in the form of black oil, while dichloromethane, ethyl acetate and anhydrous ethanol extracts were in the form of dark green, pink and white powder respectively. The average yields by petroleum ether, dichloromethane, ethyl acetate, and anhydrous ethanol were 4.4%, 2.1%, 2.3% and 2.3%, respectively. The extract yield was highest for petroleum ether, with a yield of 5.4% in May. The mortality of protoscoleces in all monthly groups of petroleum ether and dichloromethane extracts reached 100% with the concentration of 100 μg/ml and the same mortality reached in most groups of petroleum ether extracts with the concentration of 50 μg/ml. The effects of dichloromethane extracts were less than petroleum ether extracts, but significantly stronger than those of ethyl acetate and ehanol extracts. Further studies conducted on petroleum ether and dichloromethane extracts showed, the lethal effect of petroleum ether extract ranked in month of preparation from strong to weak as June>March>November>April>February>May>October>August>December>July>January>September. In June, the LC50 was 2.577 μg/ml and 95% confidence interval was 0.85-6.22 μg/ml. The lethal effect of dichlorom ethane extract ranked in month of preparation from strong to weak as November>May>October>April>July>December>June>September>August>February>March>January. In November, the LC50 was 21.85 μg/ml, and 95% confidence interval was 12.38-36.28 μg/ml.</p><p><strong>Conclusion: </strong>The Eucalyptus robusta leaves contain potential compounds against Echinococcus granulosus. Further experiments of isolation, analysis and identification are needed.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 3","pages":"266-71"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36414636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Blastocystis Infection in Pet Dogs in Selected Areas of Anhui and Zhejiang Provinces].","authors":"Wen-chao Li,&nbsp;Kai Wang,&nbsp;Mian Qin,&nbsp;Zong-hua Liu,&nbsp;Gan Yuan,&nbsp;De-yi Liu,&nbsp;You-fang Gu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To determine the prevalence and subtypes of Blastocystis in pet dogs in Anhui and Zhejiang Provinces.</p><p><strong>Methods: </strong>Pet dog fecal samples were collected from veterinary clinics in Baohe District of Hefei City, Xuanzhou District of Xuancheng City, Fengyang County and Mingguang City of Chuzhou City, Longzihu District of Bengbu City, Si County of Suzhou City in Anhui Province, as well as in Yuhang District of Hangzhou City in Zhejiang Province from April to December, 2013. Blastocystis was detected by microscopic examination as well as nested PCR and single-step PCR based on small subunit ribosomal DNA sequences. The PCR products were sequenced, and Blastocystis subtypes were assigned.</p><p><strong>Results: </strong>A total of 315 pet dog fecal samples were collected. The incidence of Blastocystis infection determined by microscopic examination, nested PCR and single-step PCR was 1.3%（4/315）, 1.9%（6/315） and 1.9%（6/315）, respectively. In addition, the two PCR methods both revealed Blastocystis infection only in Chuzhou City（3.4%, 1/29） and Hefei City（5.6%, 4/72）. The pet dogs in Chuzhou City carried ST1 and ST2 subtypes while those in Hefei carried only ST1. There was no significant difference in infection rate between adult and young dogs and between male and female dogs.</p><p><strong>Conclusion: </strong>Blastocystis infection exists in pet dogs in Chuzhou City and Hefei City.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 3","pages":"272-6"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36414638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Imaging Analysis in Cases with Clonorchiasis-Associated Cholangiocarcinoma].","authors":"Shi-chang Xu,&nbsp;Zhi-bo Wen","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the magnetic resonance imaging （MRI） and computed tomography (CT) features of clonorchiasis-associated cholangiocarcinoma, and provide reference for its clinical diagnosis.</p><p><strong>Methods: </strong>The CT and MRI （including magnetic resonance cholangiopancreatography, MRCP） data of 60 patients diagnosed to have cholangiocarcinoma（26 cases with clonorchiasis-associated cholangiocarcinoma, group A; 34 cases with simple cholangiocarcinomas, group B） by surgery or biopsy in two hospitals in Guangdong Province during July 2005 and June 2015 were collected. The tumor location, pathological types, imaging features, mode of enhancement, and the bile duct expansion were analyzed.</p><p><strong>Results: </strong>Imaging results showed that the tumor tended to occur in the right liver in group A（46.2%, 12/26） and in the left hepatic duct and the liver explorer in group B （61.8%, 21/34）（P<0.05）. The pathological types of tumor in both groups included the nodule/mass type (group A, 73.1%, 19/26; group B, 52.9%, 18/34), the infiltration type （15.4%, 4/26; 23.5%, 8/34）, and the cavity growth type（11.5%, 3/26; 23.5%, 8/34）（P>0.05）. Plain and enhanced CT and MRI results revealed no significant difference in tumor density, signal characteristics or the mode of enhancement between the two groups. MRCP results showed that the intrahepatic distal bile duct cystic dilatation, the intrahepatic bile duct cane soft tubular ectasia, the bile duct dilatation in the tumor, and the bile duct dilatation surrounding the tumor accounted for 61.5%（16/26）, 19.2% （5/26）, 50% （13/26） and 7.7%（2/26） in group A, and 8.8% （3/34）, 64.8% （22/34）, 20.6% （7/34） and 38.2% （13/34） in group B （P<0.05 for each of the four）， respectively.</p><p><strong>Conclusion: </strong>The clonorchiasis-associated cholangiocarcinoma has certain imaging characteristics. It is different from the simple cholangiocarcinomas in tumor location and intrahepatic bile duct dilatation.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 3","pages":"239-44"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36416246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[In Vitro Effects of Aminoalcohol-carbazole Compound BTB3 against Echinococcus granulosus].","authors":"Cong-shan Liu,&nbsp;Hao-bing Zhang,&nbsp;Jian Xue,&nbsp;Yi Tao,&nbsp;Wei Hu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the effects of a aminoalcohol-carbazole compound BTB3 against Echinococcus granulosus in vitro.</p><p><strong>Methods: </strong>The protoscoleces from sheep and germinal cells from secondary-infected mice were cultured and treated with 1, 2, 4, 8, 10 and 20 μg/ml BTB3 for 3 days. The viability of protoscoleces and cells was determined by the methylene blue exclusion method and CCK-8 assay. Meanwhile, the effect of 10 μg/ml BTB3 on germinal cells was assessed by scanning electron microscopy（SEM）. The metacestodes were treated with 1, 5 and 10 μg/ml BTB3 for two weeks, and the integrity of the metacestodes was evaluated by SEM.</p><p><strong>Results: </strong>The 10 μg/ml and 20 μg/ml BTB3 caused a death rate of （100.0±0.0）% and （85.2±7.2）% respectively for protoscoleces. As the concentration decreased, the death rate remained below 10%. Moreover, the activity inhibition rate on germinal cells was about 100% for 8, 10 and 20 μg/ml BTB3, and was reduced with the decrease of BTB3 concentration other than the afore-mentioned three. SEM revealed detachment, shrinkage, and cavitation of germinal cells after BTB3 treatment. And the metacestodes all showed the loss of turgidity after BTB3 treatment for 14 days, which indicated cell detachment and uneven distribution of cells in internal cyst of metacestodes.</p><p><strong>Conclusion: </strong>BTB3 has strong effects against Echinococcu granulosus protoscoleces, germinal cells and metacestodes in vitro, which make it a potential drug against hydatid diseases.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 3","pages":"245-8"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36416247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[In vitro Effect of Hypericin against Toxoplasma gondii Tachyzoites].","authors":"Xiao-di Yang,&nbsp;Xi-meng Sun,&nbsp;Qi Wang,&nbsp;Jie Cui,&nbsp;Yong-sheng Ji,&nbsp;Hong-bao Xue,&nbsp;Shou-feng Hu,&nbsp;Pang-pang Su,&nbsp;Jiang-yan Li,&nbsp;Ling-wen Meng,&nbsp;Ji-chen Qiao,&nbsp;Yi-han Ding,&nbsp;Di Song,&nbsp;Qi Wu,&nbsp;Qiang Fang,&nbsp;Xing-zhi Chen","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the killing effect of hypericin on tachyzoites of Toxoplasma gondii RH strain in vitro.</p><p><strong>Methods: </strong>Normal saline （group A） and different concentrations of hypericin (5 μg/ml, group B; 50 μg/ml, group C; 500 μg/ml, group D） were added to T. gondii tachyzoites in 24-well plate（1×10(6)/well）. The tachyzoites were harvested after 2, 4 and 6 h, and underwent the following treatment: trypan blue staining to calculate the dyeing rate, Giemsa staining to observe the morphological and structural alterations of tachyzoites, and transmission electron microscopy to observe the ultrastructure of tachyzoites. In addition, flow cytometry was performed to calculate the survival rate of YFP-carrying Toxoplasma with the same treatment.</p><p><strong>Results: </strong>The trypan blue dyeing rate at 2 h after treatment in groups B, C and D was（11.0±3.6）%, （25.0±6.3）% and（40.0±2.7）% respectively, with a significant difference of group D versus B and C （P<0.01）, and groups C and D versus group A ［（6.0±3.0）%）］. The dyeing rate at 4 h and 6 h in group D was（97.0±2.0）% and （98.0±1.7）%, respectively, both significantly higher than that of groups C ［（30.0±7.2）%, （42.7±5.5）%］, B ［（20.0±3.0）%, （34.0±6.6）%］ and A ［（10.0±1.0）%, （19.3±4.9）%］（P<0.01）. Giemsa staining showed gradual end swelling and necrosis of tachyzoites with increased treatment duration and dosage. Transmission electron microscopy showed swelling of worm body, gap between cell membrane and matrix, increase and enlargement of vacuoles inside worm body, disruption of cell membrane, and dissolving of inner structures, with increased treatment duration. Flow cytometry showed significant difference of tachyzoite survival rate at 2, 4 and 6 h after hypericin treatment with that of the control group（P<0.01）. The survival rate of group C at 2 h after hypericin treatment was（7.9±1.9）%, significantly lower than that of groups B ［（38.1±5.5）%］ and A ［（81.8±6.0）%］ （P<0.01）. No tachyzoite was found to survive in group D at 2 h and in group C at 4 h. The survival rate of group B at 4 and 6 h after hypericin treatment was（14.3±7.9）% and （1.4±1.8）%, respectively, both significantly lower than that of group A［（73.8±11.3）% and（64.1±14.4）%, respectively］ （P<0.01）.</p><p><strong>Conclusion: </strong>Hypericin has a remarkable killing effect on T. gondii tachyzoites, and the efficacy positively correlates with the dose and treatment duration.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 3","pages":"203-7"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36416403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Preparation and Molluscicidal Effect of Chlorosalicylicamide Sustained-release Granules].","authors":"Jie Qin,&nbsp;Feng-hua Wei,&nbsp;Qian Tang,&nbsp;Yi Yuan,&nbsp;Gui-ling Li,&nbsp;Xing-jian Xu,&nbsp;Min Liu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To prepare the 5% and 10% chlorosalicylicamide（quinoid-2’, 5-dichloro-4’-nitrosalicylanilide from niclosamide） sustained-release granules （LDS-SRG） and evaluate their molluscicidal effect.</p><p><strong>Methods: </strong>The 5% and 10% LDS-SRG were prepared with screened carriers, surfactants, adhesives, defoamers and lubricants. Their bulk density, water content, repose angle, critical relative humidity, thermal stability and release rate were determined. Spraying method was used to test the molluscicidal effect of LDS-SRG at 1.6 g/m2. Meanwhile, 50% wettable powder of niclosamide ethanolamine salt (WPN) was applied as the positive control at 1.0 g/m2, and dechlorinated water was used as the blank control. The mortality of snails was calculated on days 3, 7 and 14 after administration.</p><p><strong>Results: </strong>The 5% and 10% LDS-SRG were red brown in color, showed good fluidity, and had bulk density of 0.655 g/ml and 0.594 g/ml, moisture content of 1.15% and 1.28%, repose angle of 39.8° and 39.7°, and critical relative humidity of 64.98% and 61.63%, respectively. Moreover, both showed good thermal stability. The release curve was stable for both 5% and 10% LDS-SRG during day 1 to day 9, and faster release for 5% LDS-SRG than for 10% LDS-SRG. The burst release occurred on days 10 and 15, and the steady release occurred from days 14 and 20 for 5% and 10% LDS-SRG respectively. The snail mortality on day 7 after 5% LDS-SRG 1.6 g/m2 administration and on day 14 after 10% LDS-SRG 1.6 g/m2 administration was both higher than 95%, and higher than that of the 50% WPN 1.0 g/m2 control (P<0.05).</p><p><strong>Conclusion: </strong>The 5% and 10% LDS-SRG show sustained-release potential and satisfactory molluscicidal effect by spraying, reaching the evaluation standard for molluscicidal agents.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 3","pages":"235-8"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36416856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Dynamic Changes of Dectin-2 Expression on Dendritic Cells in Mice Infected with Trichinella spiralis].","authors":"Bo-yu Liu,&nbsp;Cheng Wang,&nbsp;Xin Xing,&nbsp;Hong-liang Chen,&nbsp;Jing Jiang,&nbsp;Ya-nan Cai,&nbsp;Chun-feng Wang,&nbsp;Gui-lian Yang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effect of Trichinella spiralis infection on dectin-2 expression on dendritic cells (DC) in mice.</p><p><strong>Methods: </strong>Seventy-two female BABL/c mice were randomized into experimental and control groups (n=36 in each), and received intragastric administration of 200 muscle larvae of Trichinella spiralis and PBS respectively. Six animals were each sacrificed on days 7, 14, 21, 28, 35 and 42 after administration. The spleen and mesenteric lymph nodes (MLN) were dissected and single cell suspensions were prepared. The expression of dectin-2 on DC in the spleen and MLN was examined by flow cytometry. In another experiment, bone marrow was obtained from C57BL/6 mice, and was treated with granulocyte-macrophage colony-stimulating factor and interleukin 4 to induce bone marrow-derived dendritic cells (BMDC) in vitro. Then 100 μg/ml T. spiralis excretory/secretory (ES) antigen or the same volume of PBS was added. The expression of dectin-2 was determined by flow cytometry at 6, 12, 24, 36 and 48 h.</p><p><strong>Results: </strong>The flow cytometry results showed a significant decrease of dectin-2 expression［（7.0±0.7）%］ on spleen DC compared with that of the control group［（15.1±1.6）%］（P<0.01） on day 7, and significant increase of dectin-2 on MLNDC compared with that of the control on days 7, 21 and 28 ［（11.1±3.5）% vs. （6.6±0.4）%, （12.4±1.4）% vs. （4.9±0.4）%, （6.9±1.0）% vs. （4.0±1.4）%］ （P<0.05） after infection. In vitro results showed that dectin-2 expression was （9.4±2.2）%, （6.9±1.8）%, （6.9±0.7）%, （9.1±1.9）%, and（15.9±1.9）% respectively at 6, 12, 24, 36, and 48 h after ES stimulation on BMDC. The expression at 6, 12, 24 and 36 h decreased significantly in comparison to that of the PBS group［（21.3±6.3）%］（P<0.01).</p><p><strong>Conclusion: </strong>Trichinella infection can induce changes of dectin-2 expression on DC of mice.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 2","pages":"105-8"},"PeriodicalIF":0.0,"publicationDate":"2016-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36409938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research Progress on the Genomics of Taenia solium and Candidate Vaccines for Cysticercosis].","authors":"Cheng-xu Ma,&nbsp;Hong-wei Wang,&nbsp;Yi-xuan Yang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Research on cysticercosis has been stimulated by the rapid development of genomics, proteomics and bioinformatics and the emergence of molecular biological and immunological technologies. In this review, we attempt to discuss the research development on genomics of Taenia solium and candidate vaccines and antigens for cysticercosis. This will provide a new perspective for studying genomics of Taenia solium and for cysticercosis prevention and treatment, and provide a wealth of informative data for the development of novel and highly efficient vaccines against cysticercosis or diagnostic antigen molecules for cysticercosis.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 2","pages":"161-5"},"PeriodicalIF":0.0,"publicationDate":"2016-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36411537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}