Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases最新文献

{"title":"[A Retrospective Analysis on 47 Children with Paragonimiasis in Ningbo City].","authors":"Hui-qing Xu,&nbsp;Xin-gang Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A retrospective analysis was performed on 47 children diagnosed with paragonimiasis in Ningbo Women and Children’s Hospital from January 2004 to December 2014. The 47 children comprised 32 boys （68.1%） and 15 girls （48.9%）, and 24 cases（51.1%） in urban areas and 23 （48.9%） in rural areas （P>0.05）. There was a trend of increase in paragonimiasis occurrence in preschoolers since 2010. Forty-three cases had a history of eating raw or wine-preserved crabs and 4 cases had a history of drinking raw stream water． There were 2 cases of paragonimus encephalopathy and one case accompanied by subcutaneous nodules. Thirty-nine cases showed increases in eosinophil number and proportion in peripheral blood, and 29 cases showed increased serum IgE level. Forty-seven cases had negative results for detection of paragonimus eggs in sputum and stool. The dot immuno-gold filtration-assay and ELISA showed a 100% positive rate for paragonimus serum antibody. All the 47 cases were administered with praziquantel after diagnosis, and no adverse effect was reported during the treatment.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 3","pages":"293-4"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36412010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Risk Assessment of Malaria in Libo County, Guizhou Province].","authors":"Xue-mei Zhou,&nbsp;Hai-liang Mo,&nbsp;Qi-min Liang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Data concerning malaria endemic situation during 2006-2012 and data concerning malaria transmission risk and malaria elimination capacity during 2010-2012 were collected. The results showed that during 2006-2012, malaria in Libo County was predominated by vivax malaria with the transmission vector of Anopheles sinensis, and malaria incidence declined year by year（3.72/10 000, 3.56/10 000, 5.76/10 000, 4.34/10 000, 2.54/10 000, 1.14/10 000, and 0, respectively）. In the residents surveyed during 2010-2012, >2% received blood test, 82.9%（29/35） received standard therapy, 93.3%（651/698）had usage of insect-resistant facilities, and 440 received medical training, with an awareness rate of 92.3%（738/800）in the residents. The malaria transmission risk index of Anopheles mosquito was 2, the area risk value was 10, and the malaria transmission risk index was 20, indicating a moderately-low level of risk.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 3","pages":"195-7"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36413807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Genetic Variation of Oncomelania hupensis in Weishan Lake of Shandong Province Using Microsatellite DNA Markers].","authors":"Jian He,&nbsp;Feng Miao,&nbsp;Kun Yang,&nbsp;Chang-lei Zhao,&nbsp;Xin Liu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To identify the genetic variation of Oncomelania hupensis between Weishan Lake population and Yangtze River population.</p><p><strong>Methods: </strong>O. hupensis snails were collected from Jiangdu District of Yangzhou City, Yizheng City, Danyang City, and Liuhe District of Nanjing City in Jiangsu Province, as well as Weishan Lake of Jining City in Shandong Province. Four polymorphic microsatellite DNA loci（A18, C22, T4-33 and T6-17） were amplified by using microsatellite PCR to analyze the number of alleles （Na）, the expected heterozygosity （He）, the observed heterozygosity （Ho） and the average number of pairwise differences between populations. Analyses of molecular variance (AMOVA) and cluster analysis were performed to find the genetic variation between O. hupensis snail populations. Unweighted pair group method with arithmetic mean （UPGMA） and neighbour joining (NJ) method were used to construct the phylogenetic tree to describe genetic variation and clustering among populations. Mantel test was carried out to detect the correlation between the genetic distance and geographical distance.</p><p><strong>Results: </strong>A total of 103 snails were collected. The snail populations of Jiangdu, Yizheng, Danyang, Liuhe, and Weishan Lake had Na of 7.50, 12.50, 10.00, 11.50; Ho of 0.16, 0.27, 0.17, 0.30, 0.22; and He of 0.81, 0.91, 0.84, 0.90, 0.92, respectively. The Weishan Lake population showed relatively higher levels of the three indicators, indicating a higher genetic diversity, but with no significant difference with the other four populations. The average number of pairwise differences was lowest between the Weishan Lake and Jiangdu populations（0.79）, and it was higher between Danyang population and the others（0.87-0.97）. AMOVA result showed the inter-individual genetic variation accounted for 92.50% of the total variations. NJ and UPGMA analysis showed Jiangdu and Weishan Lake populations gathered together in one branch, Yizheng and Liuhe populations formed another relatively independent branch, and then was Danyang population. Genetic distance and geographical distance showed no correlation by Mantel test.</p><p><strong>Conclusion: </strong>There is a high genetic diversity of O. hupensis in Weishan Lake and Yangtze River populations, and no significant genetic divergence between Weishan Lake snail population and its original Yangtze River population.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 3","pages":"261-5"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36414635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Diapause of Aedes albopictus and the Related Molecular Mechanisms].","authors":"Dan Xia,&nbsp;Ping-ying Teng,&nbsp;Xiao-guang Chen,&nbsp;Xiao-hong Zhou","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Aedes albopictus is an important transmitting vector of a wide range of arboviruses such as Dengue virus and Chikungunya virus. The rapid and aggressive global spread of Aedes albopictus contributes to the rapid expansion of Dengue virus. The diapause of Aedes albopictus provides a crucial ecological basis for its adaptation to a wide range of climatic and geographical conditions. This review gives an update on research progress on diapause of Aedes albopictus and the related molecular mechanisms.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 3","pages":"282-9"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36414639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Species Identification and Sequence Analysis of Plasmodium spp. in Border Areas of Yunnan Province by 18S rRNA-based Nested PCR]","authors":"Cang-lin Zhang,&nbsp;Hong-ning Zhou,&nbsp;Ren-hua Nie,&nbsp;Hui Liu,&nbsp;Jian Wang,&nbsp;Chun-fu Li,&nbsp;Ya-ming Yang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To analyze blood samples from patients with falciparum malaria and vivax malaria in border areas of Yunnan Province, using 18S rRNA-based nested PCR, and compare 18S rRNA sequences.</p><p><strong>Methods: </strong>Blood or filter blood samples with positive microscopic results for Plasmodium falciparum or P. vivax infection were collected from Laza, Nankajiang, Mangdong and Nawei of Myanmar, and from Mengla, Tengchong and Yingjiang of Yunnan Province between 2004 and 2011. 18S rRNA-based nested PCR was conducted on the samples, and PCR products were sequenced and blasted. Phylogenetic tree was constructed using the neighbor-joining method with MEGA software (version 6.06).</p><p><strong>Results: </strong>Microscopic examination revealed P. falciparum infection in 256 samples and P. vivax infection in 219 samples. The 18S rRNA-based PCR further confirmed P. falciparum infection in 242 samples, P. vivax infection in 176 samples, and mixed infection in 57 samples. The consistency rate was 81.7% （388/475） between microscopic and PCR results. The inconsistency rate significantly correlated with parasite density （Spearman’s r=-0.408， P<0.05）. Sequence alignment revealed 11 and 10 homologous sequences for P. falciparum and P. vivax 18S rRNA gene, comprising 2.9%（6/205） and 22.5%（27/120） variable sites, respectively. The 18S rRNA of P. falciparum clustered with that from Cameroon（GenBank accession number KC428742）, but was distantly related with the S-type 18S rRNA from the Netherlands （U36465） and Brazil （U36466 and U36467）. The 18S rRNA of P. vivax clustered with A-type 18S rRNA from Thailand （U07367）, but was distantly related with the C-type 18S rRNA from Thailand（U07368）.</p><p><strong>Conclusion: </strong>Nested PCR revealed mixed infection in 57 samples among those identified with single infection by microscopy. There is no significant difference in 18S rRNA sequence in seven counties/cities in Yunnan Province.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 3","pages":"220-6"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36416853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Pathogen Identification in an Imported Case of Cutaneous Leishmaniasis].","authors":"De-hua Lai,&nbsp;Na Wu,&nbsp;Yi-ting Xie,&nbsp;Xiao-kun Hong,&nbsp;Yun-fu Chen,&nbsp;Li-fu Liao,&nbsp;Zhao-rong Lun","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In this study, immune and molecular biological methods were used to identify the pathogen in a blood sample from a patient with dermatosis. Venous blood was collected and tested with Leish rK39 dipsticks. The lesion sample was collected and fixed in 75% ethanol, and DNA was extracted. The internal transcribed spacer 1 of rDNA and N-acetylglucosamine-1-phosphate transferase of Leishmania were amplified with PCR using primers LITSR-L5.8S and NAGTL1s-NAGTL4, respectively. The amplified products were sequenced and analyzed by BLAST. Weakly positive results were obtained for the gold-labeled Leish rK39 dipstick serological test. PCR resulted in products of 404 bp and 1 405 bp with primers LITSR-L5.8S and NAGTL1-NAGTL4, respectively. Both were 99.7% homologous to the corresponding sequence of Leishmania major. The accession number of the two sequences were KU975160 and KX150476. The case of dermatosis is diagnosed as imported cutaneous leishmaniasis and the pathogen is L. major.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 3","pages":"295-7"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36412011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[An Imported Case of Vivax Malaria with Co-occurrence of Different Stages of Plasmodium vivax in Erythrocyte].","authors":"Jia-zhi Wang,&nbsp;Xue-mei Yin,&nbsp;Xi-shang Li,&nbsp;Shou-qin Yin,&nbsp;Jun Feng","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The blood sample from a case of vivax malaria in Tengchong City, Yunnan Province, was tested with CareStartTM malaria rapid diagnostic test, Giemsa staining and nested PCR in 2012. The case was determined to be infected with Plasmodium vivax, P. malariae or P. ovale other than P. falciparum by CareStartTM malaria rapid diagnostic test. Microscopic results revealed multinuclear P. vivax ring form and multi-infections with P. vivax in blood slides, with occurrence of two or more nuclei in one ring form accounting for 14.68%（188/1 280）, and parasitism of two or more P. vivax rings in one erythrocyte accounting for 22.50%（288/1 280）. In addition, co-occurrence of ring form and trophozoite, and ring form and gametophyte was found in erythrocytes. Nested PCR revealed P. vivax-specific amplification products. Combining the results with epidemiological information and clinical symptoms, this case was finally diagnosed as imported vivax malaria, and the erythrocytes of the case harbored different stages of P. vivax parasites.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 3","pages":"290-2"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36414642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Polymorphism of 72-76 Coding Sequence within Exon 2 Region of Pfcrt in Yunnan Province].","authors":"Yao-ji Zhu,&nbsp;Meng-ni Chen,&nbsp;Yan-chun Xu,&nbsp;Xiang-hua Mao,&nbsp;Yan Deng,&nbsp;Ying Dong","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To understand the endemic situation of chloroquine-resistant falciparum malaria in Yunnan Province by analyzing the polymorphism of the 72-76 amino-acid coding sequence within exon 2 region of Plasmodium falciparum chloroquine resistant transporter (Pfcrt) gene （referred to as the 72-76 coding region） in malaria patients.</p><p><strong>Methods: </strong>The filter paper blood samples and relative information of falciparum malaria cases were collected in 13 prefectures of Yunnan Province （excluding Diqing, Wenshan, Zhaotong prefectures） from August 2012 to September 2015. The source of infection was determined by epidemiological investigation and the place of case discovery was confirmed according to the endemic registration in the Infectious Diseases Reporting Manage System, Chinese Center for Disease Control and Prevention. The exon2 region of Pfcrt gene was amplified by nested PCR and sequenced. The polymorphism of the 72-76 coding region was analyzed with MEGA 5.04. The variable sites and genetic distance between sequences were calculated. The constituent ratio of the polymorphism in sub-populations was analyzed with IBM SPSS Statistics 21 software.</p><p><strong>Results: </strong>Two hundred and thirty-two blood samples were collected in the period and source of infection included Yunnan of China, Africa and Myanma. Nested-PCR resulted in positive products in 210 samples. Sequence analysis showed the presence of chloroquine-sensitive genotype（CVMNK）（15.2%, 32/210） and mutated chloroquine-resistant genotype（CVIET, SVMNT and CVMNT）（76.2%, 160/210; 6.7%, 14/210; 1.9%, 4/210） 72-76 coding regions. The proportion of the CVMNK type was 100%（32/32） in cases with the range of 19-55 years, 46.9% （15/32） in farmers, and 59.4% （19/32） in patients with infection source in Southeast Asia, all significantly higher than those of other cases in the same groups（0; 31.3%, 10/32; and 37.5%, 12/32 respectively, χ2=13.674, 8.478, 6.292, P<0.05）. The proportion of the CVIET and SVMNT genotypes in patients with infection source in Myanma and Cambodia was 81.3%（130/160） and 78.6%（11/14） respectively, significantly higher than those in patients with infection source in Yunnan Province（6.3%, 10/160; 21.4%, 3/14）（χ2=6.519 and 6.620, P<0.05）. In samples with Africa infection source, the proportion of CVIET was 12.5%（20/160）, with no detection of SVMNT. There was a 145 bp homologous locus among the 210 exon2 regions, of which the conservative sites accounted for 95.2%（138/145） and variable sites for 4.8%（7/145）. The genetic distance between the 210 sequences ranged 0.000-0.036（0.012±0.005）. The genetic distances from genotypes CVIET, SVMNT and CVMNT to the chloroquine-sensitive genotype CVMNK were（0.029±0.015）, （0.021±0.013） and （0.014±0.001） respectively. 178 cases with chloroquine-resistant P. falciparum distributed in all the 13 prefectures. Among them, the regions with top detection rate of chloroquine-resistant genotypes were Dehong（51","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 3","pages":"227-32"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36416244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Interferon-γ Induced by Toxoplasma gondii Excreted/Secreted Antigens Promotes Apoptosis of CD4+CD25+ Regulatory T Cells].","authors":"Ke Ge,&nbsp;Xiao-yan Qiu,&nbsp;Li-juan Wang,&nbsp;Hui Chen,&nbsp;Lu-yan Zhang,&nbsp;Jing-fan Qiu,&nbsp;Yong Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effect of excreted/secreted antigens (ESAs) from Toxoplasma gondii RH strain and TgCtwh3 strain on apoptosis of CD4+CD25+ regulatory T cells and interferon-γ (IFN-γ) secretion.</p><p><strong>Methods: </strong>ESAs of Toxoplasma gondii RH strain and TgCtwh3 strain were prepared. Splenic mononuclear cells were isolated from C57BL/6 mice and randomly divided into RH ESA group（2×106 cells/well with addition of 10 μg/ml RH ESA）, TgCtwh3 ESA group （2×106 cells/well with addition of 10 μg/ml TgCtwh3 ESA） and control group（2×106 cells/well with addition of 10 μg/ml ovalbumin）. Flow cytometry was performed to examine the early apoptosis of CD4+CD25+ regulatory T cells after treatment for 48 h and 72 h. ELISA was conducted to determine the level of IFN-γ in the supernatant after treatment for 72 h. In another experiment, the 3 groups of splenic mononuclear cells were added with 10 μg/ml anti-IFN-γ antibody for 72 h and flow cytometry was performed to examine the early apoptosis of CD4+CD25+ regulatory T cells. Meanwhile, splenic mononuclear cells from IFN-γ knockout and wild-type C57BL/6 mice were also divided into the above-described 3 groups, and flow cytometry was performed to examine the early apoptosis of CD4+CD25+ regulatory T cells after treatment for 72 h.</p><p><strong>Results: </strong>The concentrations of RH ESA and TgCtwh3 ESA were 0.54 mg/ml and 2.14 mg/ml, respectively. Flow cytometry showed that the early apoptosis rate of CD4+CD25+ regulatory T cells in the RH ESA group and the TgCtwh3 ESA group after 48 h treatment was （12.90±1.26）% and （9.71±1.04）%, respectively （P＜0.05）, both significantly higher than that in control group （4.48±0.48）% （P<0.01） . The early apoptosis rate of CD4+CD25+ regulatory T cells after 72 h in the RH ESA group was（15.21±1.11）%, significantly higher than that in the TgCtwh3 ESA group[（11.02±0.92）%] （P<0.05） and the control group[（10.10±1.49）%]（P<0.01）. ELISA showed that the level of interferon-γ in the RH ESA group and the TgCtwh3 ESA group after 72 h was（4 764.0±118.7） pg/ml and （3 629.0±33.6） pg/ml, respectively （P＜0.01）, both significantly higher than that in the control［（679.4±30.6） pg/ml］（P<0.01）. Flow cytometry revealed lower early apoptosis rate of CD4+CD25+ regulatory T cells in the RH ESA group added with anti-IFN-γ antibody［（10.44±1.44）%］ compared with that without the addition of the antibody［（14.96±0.83）］（P<0.05）. But this difference was not observed for the TgCtwh3 ESA group. Moreover, the RH ESA-induced apoptosis rate of regulatory T cells from IFN-γ knockout mice［（10.64±0.55）%］ was significantly lower than that from the wild-type mice ［（15.21±1.11）%］（P<0.01）. But this difference was not found for the TgCtwh3 ESA treatment.</p><p><strong>Conclusion: </strong>T. gondii RH ESA induces apoptosis of CD4+CD25+ regulatory T cells and IFN-γ secretion, and these effects are stronger than those of TgCtwh3 ESA. The T. gondii ESA-induced IFN-γ stimulates generati","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 3","pages":"183-8"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36411555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Secretion and Distribution of Rhoptry Protein 16 during Toxoplasma gondii Invasion into Host Cells].","authors":"Shi-chen Jiang,&nbsp;Hai-xia Wei,&nbsp;Cheng He,&nbsp;Sheng-qun Deng,&nbsp;Jing Xia,&nbsp;Hong-juan Peng","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To examine the secretion and localization of Toxoplasma gondii Rhoptry protein 16 （ROP16） during invasion of different strains of T. gondii into host cells.</p><p><strong>Methods: </strong>The Tgrop16 gene was amplified by PCR on the cDNA of T. gondii RH strain, subcloned into the plasmid pET-32a（+）, and expressed in Escherichia coli BL21（DE3） under the induction of isopropyl β-D-1-thiogalactopyranoside. New Zealand rabbit was immuned with the expressed recombinant protein TgROP16 to produce polyclonal anti-TgROP16 antibody. The specificity and sensitivity of the polyclonal antibody were examined by Western blotting and indirect ELISA, respectively. The transcriptional and protein levels of Tgrop16 in T. gondii RH strain and Pru strain were determined by real-time PCR and Western blotting, respectively. The secretion and distribution of TgROP16 in human foreskin fibroblasts (HFFs) during the invasion by T. gondii RH strain and Pru strain were examined by indirect immunofluorescence assay (IFA).</p><p><strong>Results: </strong>Western blotting showed a specific band at M(r) of ～100 000, indicating that the specific rabbit-derived anti-TgROP16 polyclonal antibody was capable of recognizing TgROP16. Indirect ELISA revealed a titer of 1:25 600 for the antibody. The relative expression level of Tgrop16 in Pru strain［（7.786±0.206）］ was 7 times than that in RH strain［（1.000±0.110）］（P<0.05） as detected by real-time PCR, and TgROP16 protein level was higher in RH strain than in Pru strain. IFA showed that TgROP16 was localized on the apical complex of the unrecruited tachyzoite of T. gondii before invasion and was secreted out of the recruited tachyzoite after invasion.</p><p><strong>Conclusion: </strong>The anti-TgROP16 polyclonal antibody has high specificity and sensitivity. The TgROP16 protein level is higher in the RH strain than in the Pru strain. For both strains, TgROP16 is localized on the apical complex of the unrecruited tachyzoite before invasion and secreted out of the recruited tachyzoite during invasion.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 3","pages":"189-95"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36411561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}