Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases最新文献

{"title":"[Epidemiological Survey on Human Intestinal Protozoa in Xinjiang Uygur Autonomous Region in 2015].","authors":"Mamatjan Umar,&nbsp;Xiao-ying Chen,&nbsp;Yisilayin Osman,&nbsp;Adili Simayi,&nbsp;Yan-yan Hou,&nbsp;Yalikun Maimaitiyiming,&nbsp;Ning Xiao","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>An epidemiological survey was made on human intestinal protozoa in Xinjiang Uygur Autonomous Region (Xinjiang) to evaluate recent control achievements and provide basis for making specific control strategies.</p><p><strong>Methods: </strong>Regions in Xinjiang were categorized by types of ecological system and geographical characteristics into five ecological areas（types I-V） according to the National Ecological Function Stratification issued by the Ministry of Environment Protection and Chinese Academy of Science and Technology. Stratification sampling was made in each ecological area. Feces from human of all ages were collected for morphological identification of protozoan species using the Iodine Liquid Direct Smear Method.</p><p><strong>Results: </strong>In the five ecological areas, 26 886 people from 132 survey sites in 39 counties (cities) were examined, with an examination rate of 81.47% （26 886/33 000）. The infection rate was 0.32%（85/26 886）. Four species of intestinal protozoa were detected, i.e., Entamoeba histolytica, Giardia lamblia, Blastocystis hominis, and Entamoeba coli, with an infection rate of 0.22% （60/26 886）, 0.03% （9/26 886）, 0.01% （2/26 886） and 0.61% （17/26 886）, respectively. Of the five areas, the type IV area had the highest infection rate of 0.75%（28/3 758）（P<0.05）. Besides, the infection rate was higher in males （0.24%, 33/13 623） than that in females （0.39%, 52/13 263） （P<0.05）, higher in age ranges of 21-30（1.40%, 16/3 959） and 31-40 years（0.46%, 22/4 799） than other age groups （P>0.05）, higher in housewives （0.48%, 2/418） than those with other occupations, higher in the Hui group （0.61%, 15/2 445） than that in other ethnic groups, as well as highest in populations with a primary education level （0.37%, 35/9 375） and lowest in those with a college level or above（0.20%, 8/3 945）.</p><p><strong>Conclusion: </strong>The human intestinal protozoa infection was at a low level in Xinjiang in 2015.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 4","pages":"361-5"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36432997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Resistance of rAAV2-MfE77.43-Transferred Mice to Schistosoma japonicum Infection].","authors":"Hong-mei Wang,&nbsp;De-hui Xiong,&nbsp;Sai-qun Luo,&nbsp;Jie Yang,&nbsp;Yin-jun Qian,&nbsp;Zhi-qiang Qin","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the resistance of E77.43 gene of Microtus fortis（MfE77.43） to Schistosoma japonicum infection.</p><p><strong>Methods: </strong>MfE77.43 was constructed into the recombinant Adeno-associated virus AAV2. The AAV2-MfE77.43 was transfected into HEK293 cells by the calcium phosphate DNA coprecipitation method. The recombinant rAAV2-MfE77.43 was purified and total RNA was extracted from the transfected cells. The expression of E77.43 was examined by RT-PCR and the purity of rAAV2-MfE77.43 was analyzed by SDS-PAGE. Eighteen KM mice were divided into three groups （n=6 in each group）. Mice in the experiment group were intramuscularly injected on days 0, 3 and 7 with 400 μl recombinant AAV2-MfE77.43 virus which was 5-fold diluted in normal saline. Mice in negative control and blank control groups received same volume of pAAV or normal saline. Venous blood was collected through the tail before each injection, and E77.43 expression in plasma was detected by dot-ELISA method. After the last injection, each mouse was infected with 40 S. japonicum cercariae and sacrificed on day 41 after infection. Adult worms and liver eggs per gram（LEPG） were counted. Worm and egg reduction rate was calculated respectively. Egg granulomas were observed by HE staining.</p><p><strong>Results: </strong>RT-PCR resulted in a 330 bp specific band. SDS-PAGE of virus shell protein revealed three protein bands with M(r) of 87 000, 72 000 and 62 000, respectively. Dot-ELISA showed that E77.43 protein began to be expressed on day 3 after rAAV2-MfE77.43 injection, remaining stable till day 41. The adult worm number and LEPG were 20.16±3.93 and 19 800±2 715, respectively, with a worm and egg reduction rate of 27.3% and 26.2% in the experiment group. While the worm number and LEPG in the negative control group were 29.16±2.44 and 28 000±2 192（P<0.01）, respectively. HE staining and observation revealed fewer eosinophils and inflammatory cells around the liver eggs in the therapy group.</p><p><strong>Conclusion: </strong>The E77.43 gene shows protective effects against S. japonicum infection, indicating that E77.43 may participate in the natural resistance of Microtus fortis to S. japonicum infection.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 4","pages":"297-302"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36429336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Changes of Phenotype and Phagocytosis of Peritoneal Macrophages in Mice Infected with the Larval-stage of Echinococcus granulosus].","authors":"Wei Pan,&nbsp;Yu-mei Zhang,&nbsp;Fen-fen Sun,&nbsp;Jian-ping Cao,&nbsp;Yu-juan Shen","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the phenotype and phagocytosis changes of the peritoneal macrophages (Mφ) in mice infected with the larval-stage Echinococcus granulosus, and explore the role of Mφ in the responses to parasite infection.</p><p><strong>Methods: </strong>Twenty-four female BALB/c mice (age of 6-8 weeks) were randomly assigned into control group and infection group (n=12 in each group). The mice in the infection group were intraperitoneally injected with 2 000 protoscoleces, while the control mice were injected with equal volume of PBS. Five months after infection, the peritoneal mononuclear cells were collected, and the percentage of Mφ and the expression of surface markers CD40, CD80, CD86, and major histocompatibility complex Ⅱ (MHCⅡ) were determined by flow cytometry. The absorbance（A490 value） of Mφ at different concentrations（1×106, 5×105, 1×105） was determined by the neutral red assay to evaluate the phagocytic ability of Mφ.</p><p><strong>Results: </strong>The Mφ constituted（30.40±3.15）% and（20.75±5.91）% in mononuclear cells in the infection and the control groups, respectively. The percentages of Mφ expressing CD40, CD80, CD86, and MHC Ⅱ were（45.33±5.51）%, （61.00±10.61）%, （56.88±10.66）% and （27.00±3.82）% in the infection group, which were all significantly higher than those in the control ［（41.43±6.19）%, （59.23±8.65）%, （10.91±1.82）% and （13.67±3.01%）］ （P<0.05）. The A490 values of Mφ at 1×106, 5×105, 1×105 were 0.41±0.03， 0.24±0.05 and 0.16±0.01 in the infection group, which were significantly lower than those in the control （0.61±0.15, 0.47±0.07 and 0.18±0.01）（P<0.01）.</p><p><strong>Conclusion: </strong>The phagocytic ability of peritoneal Mφ is dramatically weakened after infection, but the expression of activation-associated surface markers is significantly up-regulated after infection.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 4","pages":"315-8"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36429338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Establishment of Magnetic Affinity Enzyme Linked Immunoassay Based on Schistosoma japonicum Recombinant Antigen Sj26 and Its Application in Detection of Serum Antibody with Low Intensity of Infection].","authors":"Qin Yu,&nbsp;Yan-hong Zhu,&nbsp;Fei Guan,&nbsp;Hai Yang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To develop the magnetic affinity enzyme-linked immunoassay (MEIA) using recombinant glutathione-S-transferase of Schistosoma japonicum with a relative molecular weight of 26 000（rSj26-MEIA） for antibody detection under low intensity infection.</p><p><strong>Methods: </strong>The recombinant plasmid pET28a-Sj26 was transformed into Escherichia coli strain BL21, and 0.6 mmol/L isopropyl β-D-1-thiogalactopyranoside （IPTG） was used to induce its expression. The expression products were purified by Ni2+ （nickel sulfate） affinity chromatography, SDS-PAGE and Western blotting were performed to examine the expression of rSj26. The purified rSj26 was coupled to magnetic beads as capture antigen and the reaction conditions were optimized to establish the rSj26-MEIA method. The method was then used to analyze 58 serum samples from patients with low-intensity S. japonicum infection, 30 serum samples from non-endemic areas as a negative control, and 6 serum samples from patients with paragonimus infection. Results were compared with those obtained with ELISA.</p><p><strong>Results: </strong>The concentration of purified rSj26 was 2.5 mg/ml. The rSj26 had a relative molecular weight of 27 000 and was expressed mainly in the soluble form as revealed by SDS-PAGE. It could be recognized by rabbit and murine sera infected with S. japonicum as shown by Western blotting. Optimization of rSj26-MEIA revealed that use of 0.2 mg magnetic beads loaded with 10 μg rSj26 and serum sample dilution at 1 ∶ 100 yielded the highest ratio of the mean A550 of positive serum to the mean A550 of negative sample (P/N)（3.97）. For serum samples from patients with low-intensity S. japonicum infection, rSj26-MEIA and rSj26-ELISA both resulted in a positive detection rate of 24.14%（14/58）, and P/N values of 3.61 and 2.56. In addition, Pearson′s correlation analysis revealed positive correlation between A550 values detected by rSj26-MEIA and by rSj26-ELISA（r=0.658, P<0.01）. Further, no positive reaction was found in the 6 serum samples from patients with paragonimus infection and in the 30 serum samples from non-endemic areas, either by rSj26-MEIA or by rSj26-ELISA.</p><p><strong>Conclusion: </strong>rSj26-MEIA may be used as a new technique for detection of serum antibody against S. japonicum infection with low intensity.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 4","pages":"303-7"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36429341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Interpretation of Criteria for Diagnosis of Toxoplasmosis].","authors":"Xiao-lan Yan,&nbsp;Li-yong Wen,&nbsp;Ya-yi Guan,&nbsp;Jian-feng Zhang,&nbsp;Dan-dan Lin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The criteria for Diagnosis of Toxoplasmosis (WS/T 486-2015)（referred to as the Criteria） was compiled following the Management Measures for Health Criteria and GBT 1.1-2009 Standardization Working Guidelines. The Criteria is composed of six chapters, including the range of application, terms and definitions, diagnostic principle, diagnostic standard, and differential diagnosis. Four informative appendices (etiology, epidemiology, clinical manifestation, and differential diagnosis) and one normative appendix (laboratory examination) are appended. The Criteria was issued by the National Health and Family Planning Commission of the People’s Republic of China through No.21 of Chinese Health Announcement in 2015. The Criteria provides for the first time technical reference for diagnosis of toxoplasmosis in medical institutions and disease control institutions.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 4","pages":"387-9"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36432009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Efficacy of Capture and Ligation Probe-PCR with#br# Pooling Strategy in Detection of Plasmodium spp.].","authors":"Yao-wu Zhou,&nbsp;Zu-rui Lin,&nbsp;Zhi-bin Cheng,&nbsp;Zhi Zheng,&nbsp;Xing-wu Zhou,&nbsp;Zi-you Zhou,&nbsp;Duo-quan Wang,&nbsp;Jian-xiong Li,&nbsp;Xu-can Zeng,&nbsp;Xiao-dong Sun","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The capture and ligation probe-PCR（CLIP-PCR） with pooling strategy method and microscopy were applied on 100 clinical samples（7 positive and 93 negative samples） from the malaria reference laboratory in Yunnan Province. By calculating the detection rate, sensitivity, specificity, detection time and detection cost, the efficacy of the CLIP-PCR with pooling strategy method in detecting Plasmodium spp. was evaluated. The CLIP-PCR with matrix pooling strategy successfully detected Plasmodium spp. in all the 7 positive samples. Its sensitivity and specificity relative to the microscopy as a gold standard were both 100%. The detection time for all the samples by CLIP-PCR was 5.0 h, 85.0% shorter than that by microscopy（33.3 h）, and the detection cost was 300 yuan, 75.0% less than that by microscopy （1 000 yuan）.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 4","pages":"331-3"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36432242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Pathogen Identification for An Imported Case with African Trypanosomiasis].","authors":"Yi Sun,&nbsp;Wei-hua Huang,&nbsp;Zi-guang Niu,&nbsp;Hui Wang,&nbsp;Jie Guo,&nbsp;Xiao-xia Hu,&nbsp;Jing-long Yu,&nbsp;Dao-yin Zhou,&nbsp;Ya-li Weng,&nbsp;An-mei Deng","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To perform laboratory diagnosis for an imported case of human African trypanosomiasis and identify the pathogen.</p><p><strong>Methods: </strong>Clinical and epidemiological information was collected. Blood and cerebrospinal fluid samples were collected, stained with Wright-Giemsa, and microscopically examined. Genomic DNA from the blood samples was amplified with primers specific for Trypanosoma sp. expression site-associated gene (ESAG), Trypanosoma brucei gambiense specific glycoprotein (TgsGP) and 18S rRNA（M18S-Ⅱ-Tb） gene, and Trypanosoma brucei rhodesiense specific serum resistance associated （SRA） gene. Complete blood count, blood chemistry, and CSF examination were also conducted.</p><p><strong>Results: </strong>The patient had a 4-month history of lower extremity weakness and swelling of surface lymph nodes. Physical examination showed somnolence, and occasional emotional abnormalities, accompanied by anemia (hemoglobin 85 g/L), electrolyte disturbance (sodium 124 mmol/L; chlorine 87 mmol/L) and significantly increased nonspecific immune globulin protein （globulin 63 g/L）. Epidemiological survey showed that the patient suffered insect bites and stings for several times during his work in the Republic of Gabon in Africa. Microscopic examination revealed flagella of trypanosome in peripheral blood. PCR amplification produced bands of 286, 308, and 150 bp with primers specific for ESAG, TgsGP and M18S-Ⅱ-Tb, respectively.</p><p><strong>Conclusion: </strong>The patient was diagnosed with Trypanosoma brucei gambiense infection from the clinical information, epidemiological history, etiology and PCR results.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 4","pages":"350-4"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36432635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Immunomodulatory Effect of Trichinella spiralis and its Derivative Products in Allergy and Autoimmune Diseases].","authors":"Yi-han Ding,&nbsp;Xiao-li Wang,&nbsp;Di Song,&nbsp;Qi Wu,&nbsp;Xiao-di Yang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Trichinella spiralis and its derivative antigens stimulate T cell activation by inducing dendritic cell semimaturation, thereby regulating the Th1/Th2 type immune response and alleviating or inhibiting allergy and autoimmune diseases. The regulatory T cell and anti-inflammatory factors play important roles in this immunomodulatory process. This article reviews the modulatory effects and their mechanisms of Trichinella spiralis and its derivatives in autoimmune disorders.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 4","pages":"382-6"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36432008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Hydatidosis Prevalence in 6- to 12-year-old Children in Southern Xinjiang].","authors":"Jiang-shan Zhao,&nbsp;Yan-yan Hou,&nbsp;Hai-ting Zhang,&nbsp;Ruziguli-Zhumahong","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To understand the hydatidosis prevalence in 6- to 12-year-old children in southern Xinjiang Uygur Autonomous Region and provide scientific basis for formulation of control measures.</p><p><strong>Methods: </strong>One primary school was selected from each of the pastoral area, pastoral-agricultural area, agricultural area, and township area in 42 counties （cities） of southern Xinjiang, using the stratified sampling method. With the consent of their parents, B ultrasonic abdominal scan and venous blood collection were performed on all the children （6-12 years） in the selected schools. Serum level of echinococcus antibody IgG was determined by ELISA. Hydatidosis prevalence and the serum level of IgG in these children were analyzed.</p><p><strong>Results: </strong>A total of 80 429 children were examined from 168 primary schools. B ultrasonic scan revealed hydatidosis in 9 children （0.01%）, comprising 3 cases in Kezhou, 2 in Aksu, 2 in Bazhou, and 2 in Kashi. ELISA results showed that 4 189 children were positive for serum IgG, with a positive rate of 5.21%. The positive rate was highest in Kashi （8.41%, 2 143/25 495）, followed by Aksu （5.69%, 913/16 051）, with significant difference between different areas （χ2=977.303， P<0.01）. The positive rate was lowest in 6-year children （2.13%, 44/2 065） and highest in 11-year children （5.68%, 822/14 462） （χ2=48.221, P<0.01）. In addition, the positive rate was highest in Uighur children（5.19%, 3 899/75 115）, followed by Mongolian children （4.27%, 68/1 592）, with no significant difference between ethnic groups （χ2=4.072, P>0.05）. Among the children of residents, children with a nomadic lifestyle, and children settled in winter and living in a nomadic lifestyle in summer, hydatidosis occurred in 7 residents and 2 children who settled in winter and living in a nomadic lifestyle in summer; and the positive rate of serum antibody was 5.45% （184/77 512）, 2.97% （3/101）, 0.07% （2/2 816）, respectively（χ2=148.609, P<0.01）.</p><p><strong>Conclusion: </strong>There is a low hydatidosis prevalence in 6- to 12-year-old children in southern Xinjiang, but the positive rate of serum echinococcus IgG in kashgar region, Uighur ethnicity, and children of the resident group are relatively high.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 4","pages":"370-2"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36433002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Application of Confocal Laser Scanning Microscopy in Detection of Demodex Mites].","authors":"Jia Xiao,&nbsp;Ai-yuan Guo,&nbsp;Jian Huang,&nbsp;Jing Chen","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To explore the application value of confocal laser scanning microscopy（CLSM） for detection of facial Demodex mites.</p><p><strong>Methods: </strong>One hundred and twenty patients without basic diseases visiting the Department of Dermatology of the Third Xiangya Hospital for papules, pustules, erythema, and scales and suspected to have facial demodicosis were selected. Basic information and types of facial lesion were recorded. CLSM was performed to examine Demodex infection. Twenty patients with positive infection as indicated by CLSM were randomly selected for further verification with microscopy.</p><p><strong>Results: </strong>Among the 120 patients, 59 were positive for facial demodicosis as indicated by CLSM, with a detection rate of 49.2%, 61.1% of males and 39.4% of females. The 20 patients with further microscopic examination were all diagnosed as Demodex folliculorum infection. Among the 120 patients, the Demodex detection rate showed a trend of increase with age, with the highest rate （72.7%, 16/22） in the group of 40-49 years［P<0.05 versus the 21.1% （4/19） in the group of 10-19 years and the 37.8% （14/37） in 20-29］. In addition, there was a significant difference in the percentage of patients with ＜5 mites and ≥5 mites in area of 4 mm×4 mm between type I （mainly erythema and scales）（80.6%, 29/36; 19.4%, 7/36, respectively; P<0.01） and type II lesions （mainly papules and pustules） （52.2%, 12/23; 47.8%, 11/23, respectively; P>0.05）（P<0.05）.</p><p><strong>Conclusion: </strong>CLSM is an efficient technique for detecting facial Demodex, and is advantageous as it offers in-situ, noninvasive, painless, real-time and dynamic detection.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 4","pages":"366-9"},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36432007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}