Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases最新文献

{"title":"[Exploration of Using One-step Reverse Transcription PCR in Detection of Four Species of Human Malaria Parasites].","authors":"Mei Li,&nbsp;Zhen-yu Wang,&nbsp;Tao Zhang,&nbsp;Zhi-gui Xia","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To explore the application and specificity of one-step reverse transcription PCR (RT-PCR) in detecting four species of human Plasmodium parasites.</p><p><strong>Methods: </strong>Blood samples were collected from a falciparum malaria case, a vivax malaria case, an ovale malaria case, and five quartan malaria cases. RNA and DNA were isolated. One-step RT-PCR and one-step real-time RT-PCR were performed on the RNA digested with DNase to amplify the Plasmodium 18S rRNA. Traditional PCR and one-step RT-PCR were used to amplify 18S rRNAs and 18S rDNAs in differentially diluted RNAs (with or without DNase digestion) and DNAs. The lowest detectable dilution concentrations for the two amplification systems were compared.</p><p><strong>Results: </strong>One-step RT-PCR produced specific bands of 310, 394 and 323 bp, which were sequenced to be 18S rRNA of P. falciparum, P. ovale, and P. vivax. No specific band for P. malariare was found. The one-step real-time RT-PCR results showed fluorescence for all the four species, and all had a melting curve with a single peak except for P. malariare. The lowest detectable dilution concentration by one-step RT-PCR varied from 1 to 10-4 based on the DNA or RNA template amount. Specifically, the lowest detectable dilution concentration of DNA was similar to or lower than that of original RNA by an order of magnititude, and both were lower than that of DNase-digested RNA. Further, the sensitivity of one-step RT-PCR evaluated in terms of lowest detectable dilution concentration was 10-1 000 times higher than that of the traditional PCR.</p><p><strong>Conclusions: </strong>The one-step RT-PCR technique can be applied in the detection of P. falciparum, P. ovale, and P. vivax in fresh blood samples. But its use in detecting P. malariae parasites needs further evaluation.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 6","pages":"500-5"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36426281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Malaria Situation in the People’s Republic of China in 2015].","authors":"Li Zhang,&nbsp;Jun Feng,&nbsp;Shao-sen Zhang,&nbsp;Zhi-gui Xia,&nbsp;Shui-sen Zhou","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The 2015 malaria epidemiological data reported through the annual malaria statistics reporting system were collected and analyzed. Totally 3 288 malaria cases were reported in 664 counties of 31 Provinces/Municipalities/Autonomous Regions （P/M/A） in 2015, which increased by 6.8% in comparison to that of 2014 （3 078 cases）, and the incidence in 2015 was 0.024 0/10 000. The cases were reported primarily from Provinces of Yunnan （18.4%, 606/3 288）, Jiangsu （12.3%, 405/3 288）, Sichuan （8.8%, 290/3 288）, Guangxi （7.2%, 236/3 288） and Shandong（6.4%, 212/3 288）. Of all the cases, 40（1.2%, 40/3 288） were indigenous cases, mainly distributed in the border area of Yunnan （six counties）, Tibet （one county）, Liaoning （one county） and Hainan （one county）. There was one case of whom the source of infection was unknown. The locally-infected falciparum malaria was only found in Cangyuan County of Yunnan（1 case）. The prevalence of indigenous malaria in Motuo County of the Tibet Autonomous Region was over 1/10 000. Meanwhile, there were 3 248（98.8%, 3 248/3 288） abroad-imported cases which widely distributed in the 31 P/M/As. In addition, 3 265（99.3%, 3 265/3 288） of the reported cases were confirmed in reference laboratories, comprising 878 cases of Plasmodium vivax（26.9%, 878/3 265） 1 992 cases of P. falciparum（61.0%, 1 992/3 265）, 76 cases of P. malariae（2.3%, 76/3 265）, 272 cases of P. ovale（8.3%, 272/3 265） and 47 cases of mixed infection（1.4%, 47/3 265）. Furthermore, 163 cases（5.0%, 163/3 288） with severe clinical symptoms were reported in 14 P/M/As, with 20 deaths（0.6%, 20/3 288） in 10 P/M/As. Totally 3 116 malaria cases were reported through the China Information System for Disease Control and Prevention, including 39 indigenous cases. These data reflect achievements in malaria elimination, despite that challenges remain in boarder areas of Yunnan Province and in Motuo County of the Tibet Autonomous Region. Efforts are still needed in risk assesment for malaria re-transmission.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 6","pages":"477-81"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36426346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Diagnosis and Treatment of the First Imported Case of Plasmodium knowlesi Infection in China].","authors":"Bo Pan,&nbsp;Fu-quan Pei,&nbsp;Cai-wen Ruan,&nbsp;Rong-xing Lin,&nbsp;Yong-zhen Cen,&nbsp;Meng-ran Liu,&nbsp;Zhuo-hui Deng,&nbsp;Wen-feng Ren,&nbsp;Yin-bin Liao,&nbsp;Xiao-heng Li","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To diagnose and treat the first imported active case of Plasmodium knowlesi infection in China.</p><p><strong>Methods: </strong>The clinical information of the patient was collected. Microscopy of blood smear was conducted after Giemsa staining. Genomic DNA was extracted from blood, and PCR was conducted to amplify rDNA. The PCR products were sequenced and analyzed with BLAST</p><p><strong>Results: </strong>The patient returned from a one-week tour in a tropical rain forest in Malaysia. The first disease attack occurred in Guangzhou on Oct. 16, 2014, with fever, shivering and sweating. The patient was initially diagnosed as malaria and hospitalized on Oct. 26, 2014. Microscopic observation revealed typical forms of P. knowlesi in blood smear. The red blood cells became enlarged, with big trophozoites appearing as a ring with dual cores and dark brown malaria pigment. The trophozoites were slightly bigger and thicker than P. falciparum. The schizont had 6-8 merozoites, with obvious brown malaria pigment. PCR resulted in a specific band of 1 099 bp. BLAST analysis showed that the sequence of the PCR product was 99% homologous to P. knowlesi (acession No. AM910985.1, L07560.1 and AY580317.1). The patient was diagnosed as P. knowlesi infection, and was then given an 8-day treatment with chloroquine and primaquine, together with dihydroartemisinin piperaquine phosphate tablet. The patient was discharged after recovery on Oct. 28, 2014.</p><p><strong>Conclusion: </strong>According to the clinical symptoms, epidemiological history and laboratory test, the patient has been confirmed as P. knowlesi infection. It may also be the first active case of knowlesi malaria reported in China.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 6","pages":"513-6"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36427083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Epidemiological Analysis of Imported Malaria in Tai’an City] of Shandong Province During 2011-2015].","authors":"Wei-ping Du,&nbsp;Lin-lin Zhang,&nbsp;Xin-feng Zhang,&nbsp;Yong Chen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>One hundred and sixty-three imported malaria cases were reported in Tai’an City of Shandong Province during 2011-2015, consisting of 120 cases of falciparum malaria（73.6%）, 20 vivax malaria（12.3%）, 14 ovale malaria（8.6%）, 7 quartan malaria（4.3%）, and 2 unclassified cases（1.2%）. The disease onset showed no significant seasonal variation. The reported cases were more at the age of 40～49 years（70/163, 42.9%）, and were imported mainly from Africa（156/163, 95.7%）. The median interval from symptom onset to diagnosis was 4 days, and only 15 patients（9.2%） received definite diagnosis within 24 h. After medication, 161 cases recovered and 2 cases died.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 6","pages":"505-7"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36424174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Application of Structural Biology in Research on Toxoplasma gondii].","authors":"Yan-ping Chen,&nbsp;Dong-dong Wei,&nbsp;Hong-wei Li,&nbsp;Yan-bin Wei,&nbsp;Kun Yin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Structural biology is a rapidly-developing comprehensive discipline that interprets the atom-level assembling, interaction and movement between molecules. This paper summarizes basic methods in structural biology, with a focus on structure research progress of Toxoplasma gondii rhoptry and calmodulin-like domain protein kinase, to illustrate the application of structural biology in research on important virulence factors and drug targets of T. gondii.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 6","pages":"558-64"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36424653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Infection of Giardia lamblia in HIV-Infected Individuals and in Kindergarden Children in Rural Area of Anhui and Genotype Analysis].","authors":"Ying-fang Yu,&nbsp;Xiu-ping Wu,&nbsp;Yan-hong Chu,&nbsp;Jia-xu Chen,&nbsp;Li-guang Tian","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To understand the situation of Giardia lamblia infection in HIV-infected individuals and in kindergarden children in rural area of Anhui Province and analyze the genotype of the parasite.</p><p><strong>Methods: </strong>HIV-infected individuals registered in an AIDS treatment facility and children in a local kindergarden were included in this study during April 24 and May 9, 2015. The feces were collected, stained by iodine solution, and examined by microscopy. DNA was extracted from the positive feces, and nested PCR was performed to amplify the triosephosphate isomerase（tpi） gene of G. lamblia. The products were sequenced. The phylogenetic tree was constructed with BLAST, ClustalX 1.83 and MEGA6.0 softwares for analysis of homology and phylogeny.</p><p><strong>Results: </strong>One hundred and twenty-seven HIV-infected individuals and 125 kindergarden children were included. G. lamblia infection was found in three children and one HIV-infected individual. The infection detection rate in children and HIV patients was 2.40% （3/125） and 0.79% （1/127）, respectively （P>0.05）. Feces of the three infected children was soft, and no symptoms of diarrhea and stomachache were complained. Feces of the HIV-infected individual was washy, and symptoms like diarrhea, stomachache, weakness and weight loss were reported. PCR produced a specific band at 500 bp for the four persons. The sequencing results further confirmed infection in these four persons. The duplicate samples of the infected HIV patient had a 79% sequence similarity, and were 79% and 98% homologous to the Shanghai human strain of G. lamblia (GenBank accession No: KF271445), respectively. The samples of the 3 children had a 99% similarity, and all were 79% homologous to the Shanghai human strain of G. lamblia. The phylogenetic tree showed that the isolate from the HIV patient was mixed genotype of A+B, while those from the 3 children were all assemblage A. There was a high similarity between the isolates.</p><p><strong>Conclusions: </strong>There is Giardia infections in HIV patients and kindergarden children in the area. The genotype of the isolate from the HIV individual is mixed assemblage A+B while those from the children are assemblage A.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 6","pages":"537-41"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36424949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Mutations of Plasmodium falciparum Multidrug Resistance 1 Gene in Imported Plasmodium falciparum in Wuhan].","authors":"Xi-shuai Jia,&nbsp;Shui-mao Zhou,&nbsp;Ming-xing Xu,&nbsp;Yan Yang,&nbsp;Kai Wu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To identify Plasmodium falciparum multidrug resistance 1（Pfmdr1） point mutations in imported Plasmodium falciparum in Wuhan.</p><p><strong>Methods: </strong>Blood samples were collected from returnees infected with P. falciparum in endemic areas of Africa and Myanmar during 2010-2015 in Wuhan City. Nested PCR primers were specifically designed for Pfmdr1 gene loci 86, 1042 and 1246 of P. falciparum. The Pfmdr1 gene was then amplified by nested PCR, and the products were digested by restriction enzyme ApoⅠ, AseⅠ and EcoRⅤ, respectively. The mutation rate for loci 86, 1042 and 1246 was analyzed. Results A total of 187 patients with falciparum malaria were involved in the study. Pfmdr1 was amplified from all the blood samples. Restriction enzyme digestion revealed mutation rate of 19.3%（36/187）, 4.3% （8/187） and 9.6%（18/187） for loci 86, 1042 and 1246, respectively. In detail, the mutation rate for loci 86, 1042 and 1246 was 20.6%（36/175）, 2.9%（5/175） and 10.3%（18/175） respectively in the 175 samples from Africa, and only 3 cases with locus 1042 mutation were found in the 12 samples from Myanmar.</p><p><strong>Results: </strong>A total of 187 patients with falciparum malaria were involved in the study. Pfmdr1 was amplified from all the blood samples. Restriction enzyme digestion revealed mutation rate of 19.3%（36/187）, 4.3% （8/187） and 9.6%（18/187） for loci 86, 1042 and 1246, respectively. In detail, the mutation rate for loci 86, 1042 and 1246 was 20.6%（36/175）, 2.9%（5/175） and 10.3%（18/175） respectively in the 175 samples from Africa, and only 3 cases with locus 1042 mutation were found in the 12 samples from Myanmar.</p><p><strong>Conclusion: </strong>The loci 86, 1042 and 1246 mutations of Pfmdr1 have all been found in the samples from Africa, with only one point mutation （locus 1042） found in samples from Myanmar.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 6","pages":"489-92"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36426275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Results of Soil-transmitted Nematode Monitoring in Henan Province in 2011-2015].","authors":"Yan Deng,&nbsp;Wei-qi Chen,&nbsp;Ya-lan Zhang,&nbsp;Xi-meng Lin,&nbsp;Hong-wei Zhang,&nbsp;Bian-li Xu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>To understand the endemic situation of soil-transmitted nematodiasis (Ascaris lumbricoides, Trichuris trichiura and Ancylostoma sp.) in Huaiyang County, Henan Province. Over 1 000 fecal samples from inhabitants in Huaiyang County of Henan Province were collected each year during 2011-2015, in which the soil-transmitted nematodes eggs and other intestinal helminth eggs were examined by Kato-Katz technique. The helminth-positive samples were examined by filter paper culture method to identify the species of hookworm. The cellophane swab method was used to detect Enterobius vermicularis eggs in children aged 3～12 years. Soil samples were collected from vegetable field, lavatory, courtyard and kitchen of 10 families randomly selected in each year to examine Ascaris eggs by a modified saturated sodium nitrate floatation method. In 2011-2015, 5 229 people were examined and 54 person infected with intestinal helminths were found. Five intestinal helminthes, A. lumbricoides, T. trichiura, Ancylostoma duodenale, Enterobius vermicularis and Trichostrongylus orientalis were found with 13, 2, 9, 29 and 1 infection person respectively. All showed mild infection and no multiple infections were found. There was no significant difference between the year 2015 which had the highest soil-transmitted nematode infection rate 0.6%（7/1 134） and the year 2013 which had the lowest infection rate 0.3%（3/1 037）（P>0.05）. The infection rate of intestinal helminths was highest in group of <10 years（2.8%, 25/905）, followed by the groups of >70 years（1.6%, 4/256） and 30～40 years（1.2%, 8/671）（P>0.05）. The average infection rate of E. vermicularis was 1.8%（18/993） The infection rate of E. vermicularis was relatively higher in kindergarten kids（1.6%, 6/366） and students（1.3%, 13/1 005） than that in farmers（0.3%, 10/3 782）（P<0.01）. No Ascaris eggs were found in the 200 randomly collected soil samples. The intestinal helminth infection status maintaines at low level in Henan Province during 2011-2015.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 6","pages":"533-6"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36424947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Expression and Detection of Lentivirus-mediated Green Fluorescent Protein in Schistosoma japonicum].","authors":"Yue Xin,&nbsp;Nan Zhao,&nbsp;Qing Li,&nbsp;Wei Hu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To test if the green fluorescent protein gene can be expressed in Schistosoma japonicum and produce green fluorescence signals there.</p><p><strong>Methods: </strong>The spontaneous fluorescence in Schistosoma japonicum at different developmental stages was observed by fluorescence microscopy. The cultured 14-day schistosomula were infected with the lentiviral vector containing cyto megalo virus (CMV)-driven zsGreen gene, or were incubated with RPMI 1640 medium containing 10% fetal bovine serum as a negative control. At 48 h after infection, genomic DNA and total RNA were extracted, and cDNA was synthesized. PCR was performed to detect the genomic integration of zsGreen gene and the expression of zsGreen mRNA. Green fluorescence was observed under a fluorescence microscope at 24 h and 48 h after infection, and at various time points after replacement with fresh culture medium.</p><p><strong>Results: </strong>There was no obvious spontaneous fluorescence in schistosomula, but the adult worms showed clear spontaneous fluorescence. PCR results showed a specific band of 173 bp from the schistosomula genomic DNA, which corresponded to the zsGreen gene. Gene sequencing also confirmed this result. At 24 h after infection, green fluorescence was seen in the intestine of schistosomula, which was, however, suspected to be food residues. At 48 h after infection, evenly-distributed green fluorescence emerged across the intestine, and the fluorescence was brighter than the background fluorescence of the lentiviral culture medium. The fluorescence weakened on day 3 after replacement with fresh 1640 medium, and disappeared a week later. The green fluorescence re-emerged 2 weeks after replacement with the lentivirus-containing 1640 medium. No fluorescence was seen in the negative control group.</p><p><strong>Conclusion: </strong>The exogenous green fluorescent protein gene can be expressed in Schistosoma japonicum. However, the green fluorescence seen under the fluorescence microscope needs further verification.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 6","pages":"517-21"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36427085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Analysis of Pfcrt Gene Polymorphism in Imported Falciparum Malaria Cases in Henan Province in 2015].","authors":"Rui-min Zhou,&nbsp;Yi-nan Wang,&nbsp;Dan Qian,&nbsp;Su-hua Li,&nbsp;Ying Liu,&nbsp;Cheng-yun Yang,&nbsp;Yu-ling Zhao,&nbsp;Bian-li Xu,&nbsp;Hong-wei Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To analyze Plasmodium falciparum chloroquine resistant transporter (Pfcrt) gene polymorphism in imported falciparum malaria cases in Henan Province in 2015.</p><p><strong>Methods: </strong>Blood samples were collected from 132 cases of imported falciparum malaria in Henan Province in 2015. DNA was extracted from the samples, and the Pfcrt was amplified by nested PCR using specific primers. The PCR products were digested by restriction endonuclease enzyme Apol I and sequenced. Pfcrt gene polymorphism and distribution were analyzed.</p><p><strong>Results: </strong>Most of the 132 cases of imported malaria were young male adults returning from the Africa, with the highest percentage in those from West Africa（38.6%, 51/132）, then Central Africa（26.5%, 35/132）, South Africa（25.0%, 33/132）, East Africa（8.3%, 11/132）, and North Africa（1.5%, 2/132）. The nested PCR yielded a 145-bp product for each sample, and 66.7%（88/132） of the products were completely digested by Apol I, resulting in two fragments of 114 bp and 31 bp; 32.6%（43/132） could not been digested and only a single fragment of 145 bp was shown; and 0.8%（1/132） were incompletely digested, yielding three fragments of 145 bp, 114 bp and 31 bp. By blasting against chloroquine sensitive strain 3D7, we found mutations of Pfcrt at sites correspondig to residues 74, 75 and 76 from ATG, AAT and AAA to ATT, GAA and ACA （i.e. M74I, N75E and K76T） in 43 of the 132 blood samples, and mixed type mutations into ATG/T, A/GAA/T and AA/CA at sites correspondig to residues 74, 75 and 76（CVM/I, N/E/D/K, T/K） in one blood sample. The other 88 blood samples showed a wild type with no mutation (CVMNK). Mutations occurred mainly in cases from West Africa（41.2%, 21/51）, then East Africa（36.4%, 4/11）, South Africa（30.3%, 10/33）, and Central Africa（22.9%, 8/27）（χ2=4.07, P＞0.05）. The 2 cases from the North Africa both had wild type Pfcrt; the one with mixed type mutation was from West Africa.</p><p><strong>Conclusion: </strong>Three haplotypes of Pfcrt have been found, including wild type (CVMNK), mutation type (CVIET) and mixed type (CVM/I, N/E/D/K, K/T) in the imported malaria cases. The wild type occupies the highest proportion （66.7%）, while the mutation type possesses a high proportion of 41.2% in cases from West Africa.</p>","PeriodicalId":23981,"journal":{"name":"Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases","volume":"34 5","pages":"399-404"},"PeriodicalIF":0.0,"publicationDate":"2016-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36417301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}